Click here to switch to the map view.

The map label for this gene is PurA

Identifier: 20809040

GI number: 20809040

Start: 2592956

End: 2594242

Strand: Reverse

Name: PurA

Synonym: TTE2696

Alternate gene names: 20809040

Gene position: 2594242-2592956 (Counterclockwise)

Preceding gene: 20809041

Following gene: 20809039

Centisome position: 96.46

GC content: 39.78

Gene sequence:

>1287_bases
ATGTCTGTTACTGTGATTGTGGGCGCTCAATGGGGCGATGAAGGAAAAGGGAAGATAACGGATTATCTTGCAGAGAGGTC
AGAAGTGGTGGTGAGGTACCAAGGAGGCAATAATGCAGGGCATACTGTAGAAAAAGATGGTGTTCAGTATAAGCTTCATC
TCATTCCTTCTGGAATACTATACCCTGACAAAATATGTGTAATTGGCAATGGGGTAGTAGTTGATCCTTCGGCTCTTTTG
AAGGAGATTGAGGATTTAAAAGCTAAAGGAGTGGAAGTGAAAGAGGATAATTTAAAAATCAGCGATAGAGCCCATGTAGT
TTTTCCCTATCACATAAAAGAAGATGAACTAGAAGAAAAAGGAAAGGGCGATGAGGACTTAGGGACTACAAAAAAAGGAA
TAGGGCCTTGCTATAGGGATAAGACAGAGAGAATAGGCATAAGAATGTGTGACCTTATGAATGCCGAGGTTTTCAGGGAG
AAGCTTAAAAAGAATCTAGAGAGAAAAAATAAAATTTTTAAAGATATTTACGGTGAAGATGGATTTGATTTCGAGCAAAT
CTTTGAAACTTACAGGGAGTATGCAGAAAGGCTGAGACCTTATGTGACTGATACTTCTGTTCTGCTTTACAATTTAGCAA
AGGAAGGTAAAAAAATCTTGTTTGAAGGGGCTCAAGGAACTCTTCTAGACTTGGATTTTGGTACTTATCCCTATGTTACG
GCTTCTCATCCTGTGGCTGGAGGAGCTACTATAGGAGCGGGTATAGGGCCTACGATGATTGACAATGTGATAGGTGTTGT
CAAAGCGTATACTACGAGGGTAGGAAAGGGACCTTTTCCTACAGAACTTAAAGATGAAATTGGAGATTTTTTGAGAGAAA
AAGGCTATGAATATGGGACAACTACTGGAAGGCCTAGAAGGTGCGGATGGATAGATATTGTAATGCTAAGATATGCTGTC
AGAGTCTCTGGCATAACGAGCCTTGCTCTCACAAAGCTAGATACTCTGACAGGACTTGAAAAAATAAAAATATGCACAGG
GTACAAAATTAACGGAAAGATTATTGAGGATTTCCCGGCAAGCCTCGAAGAATTGAAAATGTGTGAACCCATATACGAAG
AAATGGAGGGCTGGAGTGAGAATATACAGGATGTGAGAAGCTTTGAAGACTTACCTTTGAATGCGAAAAAATATGTAAAA
AGGTTGGAAGAACTCGTAGGAGTGGAATTTAGCATAATTTCTGTAGGTCCCGAAAGAGAAGAGACTATCGTTTTGAGGAA
TTTTTGA

Upstream 100 bases:

>100_bases
GAGCTGGCATAACGAGAGGCTTAGCTCAGGCTTCAGTTTCAGGGGTTGTTGTGGCAAGGGAAATCTTGGAGAGAGTCAAA
TAAAGGAAGGTGAAATAAAT

Downstream 100 bases:

>100_bases
TAAAAAGCTTGACATTATACCCTCGGGTAAGTATAATATATAACTGTCAGGAGAATTTAATAGGTAAAAAGGTGCTGGCG
TAGCTCAATAGGTAGAGCAG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSVTVIVGAQWGDEGKGKITDYLAERSEVVVRYQGGNNAGHTVEKDGVQYKLHLIPSGILYPDKICVIGNGVVVDPSALL
KEIEDLKAKGVEVKEDNLKISDRAHVVFPYHIKEDELEEKGKGDEDLGTTKKGIGPCYRDKTERIGIRMCDLMNAEVFRE
KLKKNLERKNKIFKDIYGEDGFDFEQIFETYREYAERLRPYVTDTSVLLYNLAKEGKKILFEGAQGTLLDLDFGTYPYVT
ASHPVAGGATIGAGIGPTMIDNVIGVVKAYTTRVGKGPFPTELKDEIGDFLREKGYEYGTTTGRPRRCGWIDIVMLRYAV
RVSGITSLALTKLDTLTGLEKIKICTGYKINGKIIEDFPASLEELKMCEPIYEEMEGWSENIQDVRSFEDLPLNAKKYVK
RLEELVGVEFSIISVGPEREETIVLRNF

Sequences:

>Translated_428_residues
MSVTVIVGAQWGDEGKGKITDYLAERSEVVVRYQGGNNAGHTVEKDGVQYKLHLIPSGILYPDKICVIGNGVVVDPSALL
KEIEDLKAKGVEVKEDNLKISDRAHVVFPYHIKEDELEEKGKGDEDLGTTKKGIGPCYRDKTERIGIRMCDLMNAEVFRE
KLKKNLERKNKIFKDIYGEDGFDFEQIFETYREYAERLRPYVTDTSVLLYNLAKEGKKILFEGAQGTLLDLDFGTYPYVT
ASHPVAGGATIGAGIGPTMIDNVIGVVKAYTTRVGKGPFPTELKDEIGDFLREKGYEYGTTTGRPRRCGWIDIVMLRYAV
RVSGITSLALTKLDTLTGLEKIKICTGYKINGKIIEDFPASLEELKMCEPIYEEMEGWSENIQDVRSFEDLPLNAKKYVK
RLEELVGVEFSIISVGPEREETIVLRNF
>Mature_427_residues
SVTVIVGAQWGDEGKGKITDYLAERSEVVVRYQGGNNAGHTVEKDGVQYKLHLIPSGILYPDKICVIGNGVVVDPSALLK
EIEDLKAKGVEVKEDNLKISDRAHVVFPYHIKEDELEEKGKGDEDLGTTKKGIGPCYRDKTERIGIRMCDLMNAEVFREK
LKKNLERKNKIFKDIYGEDGFDFEQIFETYREYAERLRPYVTDTSVLLYNLAKEGKKILFEGAQGTLLDLDFGTYPYVTA
SHPVAGGATIGAGIGPTMIDNVIGVVKAYTTRVGKGPFPTELKDEIGDFLREKGYEYGTTTGRPRRCGWIDIVMLRYAVR
VSGITSLALTKLDTLTGLEKIKICTGYKINGKIIEDFPASLEELKMCEPIYEEMEGWSENIQDVRSFEDLPLNAKKYVKR
LEELVGVEFSIISVGPEREETIVLRNF

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI34577063, Length=430, Percent_Identity=47.4418604651163, Blast_Score=386, Evalue=1e-107,
Organism=Homo sapiens, GI22748717, Length=428, Percent_Identity=47.4299065420561, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI40316944, Length=397, Percent_Identity=46.3476070528967, Blast_Score=341, Evalue=7e-94,
Organism=Escherichia coli, GI1790620, Length=424, Percent_Identity=48.1132075471698, Blast_Score=414, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI25146555, Length=435, Percent_Identity=47.816091954023, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI25146553, Length=435, Percent_Identity=47.816091954023, Blast_Score=361, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6324109, Length=431, Percent_Identity=45.0116009280742, Blast_Score=379, Evalue=1e-106,
Organism=Drosophila melanogaster, GI21356033, Length=423, Percent_Identity=47.2813238770686, Blast_Score=387, Evalue=1e-108,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_THETN (Q8R6T8)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_624211.1
- ProteinModelPortal:   Q8R6T8
- SMR:   Q8R6T8
- GeneID:   996768
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE2696
- NMPDR:   fig|273068.3.peg.2263
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   PRK01117
- BioCyc:   TTEN273068:TTE2696-MONOMER
- BRENDA:   6.3.4.4
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 48026; Mature: 47894

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 130-130 BINDING 144-144 BINDING 225-225 BINDING 240-240 BINDING 304-304 BINDING 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTVIVGAQWGDEGKGKITDYLAERSEVVVRYQGGNNAGHTVEKDGVQYKLHLIPSGIL
CEEEEEEECCCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCC
YPDKICVIGNGVVVDPSALLKEIEDLKAKGVEVKEDNLKISDRAHVVFPYHIKEDELEEK
CCCCEEEECCCEEECHHHHHHHHHHHHHCCCEEECCCEEEECCEEEEEEEECCHHHHHHC
GKGDEDLGTTKKGIGPCYRDKTERIGIRMCDLMNAEVFREKLKKNLERKNKIFKDIYGED
CCCCCCCCCCHHCCCCHHCCHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCC
GFDFEQIFETYREYAERLRPYVTDTSVLLYNLAKEGKKILFEGAQGTLLDLDFGTYPYVT
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCEEE
ASHPVAGGATIGAGIGPTMIDNVIGVVKAYTTRVGKGPFPTELKDEIGDFLREKGYEYGT
CCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCC
TTGRPRRCGWIDIVMLRYAVRVSGITSLALTKLDTLTGLEKIKICTGYKINGKIIEDFPA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEECEEECCEECCCHHC
SLEELKMCEPIYEEMEGWSENIQDVRSFEDLPLNAKKYVKRLEELVGVEFSIISVGPERE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEEECCCCCC
ETIVLRNF
CEEEEECC
>Mature Secondary Structure 
SVTVIVGAQWGDEGKGKITDYLAERSEVVVRYQGGNNAGHTVEKDGVQYKLHLIPSGIL
EEEEEEECCCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCC
YPDKICVIGNGVVVDPSALLKEIEDLKAKGVEVKEDNLKISDRAHVVFPYHIKEDELEEK
CCCCEEEECCCEEECHHHHHHHHHHHHHCCCEEECCCEEEECCEEEEEEEECCHHHHHHC
GKGDEDLGTTKKGIGPCYRDKTERIGIRMCDLMNAEVFREKLKKNLERKNKIFKDIYGED
CCCCCCCCCCHHCCCCHHCCHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCC
GFDFEQIFETYREYAERLRPYVTDTSVLLYNLAKEGKKILFEGAQGTLLDLDFGTYPYVT
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCEEE
ASHPVAGGATIGAGIGPTMIDNVIGVVKAYTTRVGKGPFPTELKDEIGDFLREKGYEYGT
CCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCC
TTGRPRRCGWIDIVMLRYAVRVSGITSLALTKLDTLTGLEKIKICTGYKINGKIIEDFPA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEECEEECCEECCCHHC
SLEELKMCEPIYEEMEGWSENIQDVRSFEDLPLNAKKYVKRLEELVGVEFSIISVGPERE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEEECCCCCC
ETIVLRNF
CEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336