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The map label for this gene is PepN [H]

Identifier: 20809038

GI number: 20809038

Start: 2589816

End: 2591255

Strand: Reverse

Name: PepN [H]

Synonym: TTE2694

Alternate gene names: 20809038

Gene position: 2591255-2589816 (Counterclockwise)

Preceding gene: 20809039

Following gene: 20809035

Centisome position: 96.35

GC content: 38.89

Gene sequence:

>1440_bases
ATGGATGTCAAGAAAAAATTGCTTTTGGGGCTTTTAGCAGTAGTTATAGCTGTTTTATTTGTGTATTTTGCGCTTCCTGA
TAAGGGAATGACTACATATCTCATTGAGGCAAAATATGACGATGGAAGCAAAACAGTAGAAGCTGTTCAAACAGTCAGAT
ATGTAAACACAACTGGTGCTTCCCTTAAAGAGATTTGCTTTCACCTCTATCCCAACGCTTTCAAGTCTGAAGAAAATCTC
CCATTTACAAAGGAAGAACTTCCTTTTGTTTATCCTGAAGGCTTTGCTCCCGGTTTCATTGAGATTGAAAAGGTCACGAT
AAACGGCCTTCCTGCTGTATACACTCTGGAAGAGCCCAATGATGAGATTTTGAGATTGACATTGCCTAGAGAGATTAAAA
AAGGAGAATCTGTCAATATAAAGATGCAGTATAAGGTGAAGCTTCCTCCCGCAAGGAGCAGATTTGGGTACGGCAAAAAT
ACTGTTCAATTGGGAAACTGGTATCCCATTCTCTCAGTTTACGACAAGAACGGATGGAATAACGACCCGTATTATATTTT
CGGCGACCCATTTTACAGTGACATAGCCGATTACAAGGTAGAGTTGACTGTTCCAGAAAAGTTTGTAGTTGCTTGTACAG
GGGAAGAGAAAGAGCAAAAAGTGGAAAAGGGTTCTAAAAAACTGGTGATTGAGGCTCATAAGGTGAGAGATTTCGCAATT
GTCATGAGCGATAAGTTCAAAGTGATAGAGCAAGTGGTGGACGGGATAAAGGTGAAGTCTTACTATTTTACAGAAGGGTA
TGGAGATAAGGTTTTGAAGTTCGCCTCTGATGCCATTAAGTTTTACAATGACTACATTGGCAAATATCCTTACAAAGAAT
ATTCTGTGGTAGAGACTGATTTTTACATGGGAGGAATGGAATATCCGCAGCTTGTGATGATATCTAAAGATTTGTACAAT
AAGGCTAATCTTTTCAACCTCGAATATGTTATCGCTCACGAAACAGCTCATCAGTGGTGGTATGCATTAGTTGGAAACAA
CCAGATAAGGGAATCGTGGCTGGATGAAGGGCTCACAGAATACACCACTCTCCTTTACATAGAGAGATACTACGGAAAGG
CTACAGCAGAGGAGATATACAGGCTTGTAATTGAGGGAGGGTTTAATAAGTTTGTAAATAACAGCGCGGATACTTCTTTT
GCAAAAACTCTGGCTGATTTTAAAGAATGGAAAGAATATACAAATATAGCTTACAACAAAGGAGCAATGGTTTTTTACAA
TTTAAGAAAATTAATAGGTGATGAAGACTTCAAGAAAGTGTTACAGACCTACTACGAAAAGTACAAATACAAAATTGCCA
CTACGCAAGATCTGATTGACGTAGTGGACAGCGTAACAGGAAAAGATACAGGGGGATTTTTCCAGTCATGGTTTTATTAG

Upstream 100 bases:

>100_bases
TGAGTGCTTTTTGGCACTTTATTTTTTTATTAAAGTTGTTCTAATGTGGGCTTCCCTTAACTAATATATAATGTGAGGAT
AATTTAGGGGAGCTGGTATT

Downstream 100 bases:

>100_bases
ATTATCATCTTTTTCTTTAACCTTATGTCCTCTCCGTAAAAGTTGAGGAGCTTGAAATGACCAAAGATGCGCGAATAAAT
TCTTTCCGGGTATATAGCCA

Product: aminopeptidase N

Products: NA

Alternate protein names: Alanine aminopeptidase; Lysyl aminopeptidase; Lys-AP [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MDVKKKLLLGLLAVVIAVLFVYFALPDKGMTTYLIEAKYDDGSKTVEAVQTVRYVNTTGASLKEICFHLYPNAFKSEENL
PFTKEELPFVYPEGFAPGFIEIEKVTINGLPAVYTLEEPNDEILRLTLPREIKKGESVNIKMQYKVKLPPARSRFGYGKN
TVQLGNWYPILSVYDKNGWNNDPYYIFGDPFYSDIADYKVELTVPEKFVVACTGEEKEQKVEKGSKKLVIEAHKVRDFAI
VMSDKFKVIEQVVDGIKVKSYYFTEGYGDKVLKFASDAIKFYNDYIGKYPYKEYSVVETDFYMGGMEYPQLVMISKDLYN
KANLFNLEYVIAHETAHQWWYALVGNNQIRESWLDEGLTEYTTLLYIERYYGKATAEEIYRLVIEGGFNKFVNNSADTSF
AKTLADFKEWKEYTNIAYNKGAMVFYNLRKLIGDEDFKKVLQTYYEKYKYKIATTQDLIDVVDSVTGKDTGGFFQSWFY

Sequences:

>Translated_479_residues
MDVKKKLLLGLLAVVIAVLFVYFALPDKGMTTYLIEAKYDDGSKTVEAVQTVRYVNTTGASLKEICFHLYPNAFKSEENL
PFTKEELPFVYPEGFAPGFIEIEKVTINGLPAVYTLEEPNDEILRLTLPREIKKGESVNIKMQYKVKLPPARSRFGYGKN
TVQLGNWYPILSVYDKNGWNNDPYYIFGDPFYSDIADYKVELTVPEKFVVACTGEEKEQKVEKGSKKLVIEAHKVRDFAI
VMSDKFKVIEQVVDGIKVKSYYFTEGYGDKVLKFASDAIKFYNDYIGKYPYKEYSVVETDFYMGGMEYPQLVMISKDLYN
KANLFNLEYVIAHETAHQWWYALVGNNQIRESWLDEGLTEYTTLLYIERYYGKATAEEIYRLVIEGGFNKFVNNSADTSF
AKTLADFKEWKEYTNIAYNKGAMVFYNLRKLIGDEDFKKVLQTYYEKYKYKIATTQDLIDVVDSVTGKDTGGFFQSWFY
>Mature_479_residues
MDVKKKLLLGLLAVVIAVLFVYFALPDKGMTTYLIEAKYDDGSKTVEAVQTVRYVNTTGASLKEICFHLYPNAFKSEENL
PFTKEELPFVYPEGFAPGFIEIEKVTINGLPAVYTLEEPNDEILRLTLPREIKKGESVNIKMQYKVKLPPARSRFGYGKN
TVQLGNWYPILSVYDKNGWNNDPYYIFGDPFYSDIADYKVELTVPEKFVVACTGEEKEQKVEKGSKKLVIEAHKVRDFAI
VMSDKFKVIEQVVDGIKVKSYYFTEGYGDKVLKFASDAIKFYNDYIGKYPYKEYSVVETDFYMGGMEYPQLVMISKDLYN
KANLFNLEYVIAHETAHQWWYALVGNNQIRESWLDEGLTEYTTLLYIERYYGKATAEEIYRLVIEGGFNKFVNNSADTSF
AKTLADFKEWKEYTNIAYNKGAMVFYNLRKLIGDEDFKKVLQTYYEKYKYKIATTQDLIDVVDSVTGKDTGGFFQSWFY

Specific function: Aminopeptidase with broad substrate specificity to several peptides. It has more affinity for oligopeptides than for dipeptides. It plays an essential role in the metabolism, it may be involved in nitrogen supply or protein turnover [H]

COG id: COG0308

COG function: function code E; Aminopeptidase N

Gene ontology:

Cell location: Cytoplasm. Note=It may be secreted through an unknown mechanism [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M1 family [H]

Homologues:

Organism=Homo sapiens, GI4505029, Length=397, Percent_Identity=26.448362720403, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI158937236, Length=239, Percent_Identity=26.7782426778243, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI94818901, Length=253, Percent_Identity=24.1106719367589, Blast_Score=66, Evalue=6e-11,
Organism=Homo sapiens, GI94818891, Length=253, Percent_Identity=24.1106719367589, Blast_Score=66, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI71984353, Length=192, Percent_Identity=28.125, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71984339, Length=192, Percent_Identity=28.125, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17544504, Length=190, Percent_Identity=30, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321837, Length=256, Percent_Identity=25, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001930
- InterPro:   IPR014782 [H]

Pfam domain/function: PF01433 Peptidase_M1 [H]

EC number: =3.4.11.2 [H]

Molecular weight: Translated: 55427; Mature: 55427

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVKKKLLLGLLAVVIAVLFVYFALPDKGMTTYLIEAKYDDGSKTVEAVQTVRYVNTTGA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCCC
SLKEICFHLYPNAFKSEENLPFTKEELPFVYPEGFAPGFIEIEKVTINGLPAVYTLEEPN
CHHHHHHHHCCCHHCCCCCCCCCHHHCCEECCCCCCCCEEEEEEEEECCCCEEEEECCCC
DEILRLTLPREIKKGESVNIKMQYKVKLPPARSRFGYGKNTVQLGNWYPILSVYDKNGWN
CCEEEEECCHHHCCCCCEEEEEEEEEECCCCCCCCCCCCCEEEECCEEEEEEEEECCCCC
NDPYYIFGDPFYSDIADYKVELTVPEKFVVACTGEEKEQKVEKGSKKLVIEAHKVRDFAI
CCCEEEECCCHHHCCCCCEEEEECCCEEEEEECCCHHHHHHHCCCCEEEEEEECCEEEEE
VMSDKFKVIEQVVDGIKVKSYYFTEGYGDKVLKFASDAIKFYNDYIGKYPYKEYSVVETD
EECHHHHHHHHHHCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEH
FYMGGMEYPQLVMISKDLYNKANLFNLEYVIAHETAHQWWYALVGNNQIRESWLDEGLTE
EEECCCCCCEEEEEEHHHCCCCCEEEEEEEEEECCHHHEEEEEECCCHHHHHHHHCCHHH
YTTLLYIERYYGKATAEEIYRLVIEGGFNKFVNNSADTSFAKTLADFKEWKEYTNIAYNK
HHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCEECC
GAMVFYNLRKLIGDEDFKKVLQTYYEKYKYKIATTQDLIDVVDSVTGKDTGGFFQSWFY
CCEEEEHHHHHHCCHHHHHHHHHHHHHHEEEEECHHHHHHHHHHCCCCCCCCHHHHCCC
>Mature Secondary Structure
MDVKKKLLLGLLAVVIAVLFVYFALPDKGMTTYLIEAKYDDGSKTVEAVQTVRYVNTTGA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCCC
SLKEICFHLYPNAFKSEENLPFTKEELPFVYPEGFAPGFIEIEKVTINGLPAVYTLEEPN
CHHHHHHHHCCCHHCCCCCCCCCHHHCCEECCCCCCCCEEEEEEEEECCCCEEEEECCCC
DEILRLTLPREIKKGESVNIKMQYKVKLPPARSRFGYGKNTVQLGNWYPILSVYDKNGWN
CCEEEEECCHHHCCCCCEEEEEEEEEECCCCCCCCCCCCCEEEECCEEEEEEEEECCCCC
NDPYYIFGDPFYSDIADYKVELTVPEKFVVACTGEEKEQKVEKGSKKLVIEAHKVRDFAI
CCCEEEECCCHHHCCCCCEEEEECCCEEEEEECCCHHHHHHHCCCCEEEEEEECCEEEEE
VMSDKFKVIEQVVDGIKVKSYYFTEGYGDKVLKFASDAIKFYNDYIGKYPYKEYSVVETD
EECHHHHHHHHHHCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEH
FYMGGMEYPQLVMISKDLYNKANLFNLEYVIAHETAHQWWYALVGNNQIRESWLDEGLTE
EEECCCCCCEEEEEEHHHCCCCCEEEEEEEEEECCHHHEEEEEECCCHHHHHHHHCCHHH
YTTLLYIERYYGKATAEEIYRLVIEGGFNKFVNNSADTSFAKTLADFKEWKEYTNIAYNK
HHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCEECC
GAMVFYNLRKLIGDEDFKKVLQTYYEKYKYKIATTQDLIDVVDSVTGKDTGGFFQSWFY
CCEEEEHHHHHHCCHHHHHHHHHHHHHHEEEEECHHHHHHHHHHCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA