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The map label for this gene is SpoU3 [H]

Identifier: 20808975

GI number: 20808975

Start: 2522709

End: 2523341

Strand: Reverse

Name: SpoU3 [H]

Synonym: TTE2630

Alternate gene names: 20808975

Gene position: 2523341-2522709 (Counterclockwise)

Preceding gene: 20808976

Following gene: 20808974

Centisome position: 93.82

GC content: 36.49

Gene sequence:

>633_bases
ATGCAATCTAACAGAATAATAGTTTTAGAAGATAGGCTAAAAAGATTTTATGAAGTCTTACAGAAAAGGCAAAAAGACCT
TGTCGTTTTTGTGGATGATGTGACGAACGAGCACAATTTTTCGGCGATATTAAGGACATGCGATGCTGTTGGAGTGATGA
GAGTATACTATTTTTCTGAAGCAAAAAAGGGCATAGAGATAAATGAAGCGGTGTCAATGGCAGCAAATAGGTGGCTCTTT
ATTGAAAGGGTTTATGACAGAGAAAAGGCGATAAAGGAGATAAAAGAAAGAGAAAATTTGCAGGTTGTTGTCACTTGGCT
AGATGAGTCATCAAAAGATTTCCGGGAAATAAATTATACAAAGCCTACTTTATTGGTGGTTGGGAATGAACTAAAAGGCG
TGTCGGAGGATATATTAAATCTTGCGGATGAAAGAATTGTTATACCTATGATGGGTATGGTGCAAAGCCTGAATGTATCT
GTGGCGACAGGCATAATACTTTATGAGGCTTTAAGGCAGAGGCTTGATAAAGGCATGTATTTAAAGCCAACCCTTTCAGA
AAAGGAAATTGAAGAAATTATAATGAAGTGGAATAATGACATAATCGCTAGGAGAAAAGAAAGGAGAAAATAA

Upstream 100 bases:

>100_bases
TTGTGTGAAAGGGCATACTAAGCTACTTTCAAACACACCTTTATACGCTGTAAATTATCTTTTAAAAACATAAAATTTCA
CACAAGGCAGGGAGATCTTT

Downstream 100 bases:

>100_bases
GAGTGCGAAGAGGGGAAGTGCTTGCTCTTTCCGCAGGTGCCCTGTGGGGAACGACAGGTCTTTTTGTCAGGATGATAGTT
AAATTGGGTTTTACTACCGC

Product: rRNA methylase

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MQSNRIIVLEDRLKRFYEVLQKRQKDLVVFVDDVTNEHNFSAILRTCDAVGVMRVYYFSEAKKGIEINEAVSMAANRWLF
IERVYDREKAIKEIKERENLQVVVTWLDESSKDFREINYTKPTLLVVGNELKGVSEDILNLADERIVIPMMGMVQSLNVS
VATGIILYEALRQRLDKGMYLKPTLSEKEIEEIIMKWNNDIIARRKERRK

Sequences:

>Translated_210_residues
MQSNRIIVLEDRLKRFYEVLQKRQKDLVVFVDDVTNEHNFSAILRTCDAVGVMRVYYFSEAKKGIEINEAVSMAANRWLF
IERVYDREKAIKEIKERENLQVVVTWLDESSKDFREINYTKPTLLVVGNELKGVSEDILNLADERIVIPMMGMVQSLNVS
VATGIILYEALRQRLDKGMYLKPTLSEKEIEEIIMKWNNDIIARRKERRK
>Mature_210_residues
MQSNRIIVLEDRLKRFYEVLQKRQKDLVVFVDDVTNEHNFSAILRTCDAVGVMRVYYFSEAKKGIEINEAVSMAANRWLF
IERVYDREKAIKEIKERENLQVVVTWLDESSKDFREINYTKPTLLVVGNELKGVSEDILNLADERIVIPMMGMVQSLNVS
VATGIILYEALRQRLDKGMYLKPTLSEKEIEEIIMKWNNDIIARRKERRK

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=200, Percent_Identity=39, Blast_Score=153, Evalue=9e-39,
Organism=Escherichia coli, GI1790623, Length=153, Percent_Identity=35.2941176470588, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 24675; Mature: 24675

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSNRIIVLEDRLKRFYEVLQKRQKDLVVFVDDVTNEHNFSAILRTCDAVGVMRVYYFSE
CCCCCEEEEHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
AKKGIEINEAVSMAANRWLFIERVYDREKAIKEIKERENLQVVVTWLDESSKDFREINYT
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHCCCC
KPTLLVVGNELKGVSEDILNLADERIVIPMMGMVQSLNVSVATGIILYEALRQRLDKGMY
CCEEEEECCCCCCHHHHHHHHHCCEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCE
LKPTLSEKEIEEIIMKWNNDIIARRKERRK
ECCCCCHHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MQSNRIIVLEDRLKRFYEVLQKRQKDLVVFVDDVTNEHNFSAILRTCDAVGVMRVYYFSE
CCCCCEEEEHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
AKKGIEINEAVSMAANRWLFIERVYDREKAIKEIKERENLQVVVTWLDESSKDFREINYT
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHCCCC
KPTLLVVGNELKGVSEDILNLADERIVIPMMGMVQSLNVSVATGIILYEALRQRLDKGMY
CCEEEEECCCCCCHHHHHHHHHCCEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCE
LKPTLSEKEIEEIIMKWNNDIIARRKERRK
ECCCCCHHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]