Click here to switch to the map view.

The map label for this gene is ApbE [H]

Identifier: 20808837

GI number: 20808837

Start: 2368859

End: 2369884

Strand: Reverse

Name: ApbE [H]

Synonym: TTE2482

Alternate gene names: 20808837

Gene position: 2369884-2368859 (Counterclockwise)

Preceding gene: 20808838

Following gene: 20808836

Centisome position: 88.12

GC content: 32.26

Gene sequence:

>1026_bases
ATGAAAAAATTTATAGTACTTTTTAGTATTTTTCTACTAATATTTATATTTTCTGGGTGTTCAAATAAAAATTCTCAACC
TATATCTGATACTAAATTTGCACTGGATACTTATTGTACTTTGACTGTATACGACAAGGTTCCCAAAAAAGTATTGGATG
ATGGATTTAAAGCCATTGAAGATATAGAAAAGAAAATGAGCGTCACTATAGAGGATAGTGAAGTAAGTGAAATTAATAAA
AACGCAGGGATTAGACCCGTAAAAGTGTCTCCTGATACTTTTTACGTTATAAAAAAAGCAATACATTTTTCTGAAATTGA
AAAAGGTTATTTTGATATCACGGTAGGTCCTATAGTAAATCTTTGGAATATTGGTACCGATAAAGCCAGAGTGCCTTCAG
CGGAAGAAATAAAAGCTAAACTTCCTCTTGTAAACTATAAAAATATAGTTTTAGATGAAAAGAATATGACCGTTATGTTA
AAAGATAAAGGTATGAGCATAGACTTGGGAGGTATTGCGAAAGGCTATGCTGCAGATAAAGTGGCAGAAGTTTTAAAAAA
AGAAGGTGTAAAGCATGCTATTATAAATTTGGGCGGTGATGTCCTTACAATTGGTACAAAACCTGATGGAACTAATTGGC
GCGTAGGGATTCAAACTCCTTTTAAACCCAGAGGTGAATATTTTGGGATAGTAGAGGTTGGTGAAAAAGCTGTCGTCACT
TCAGGGATATATGAAAGATATTTTGAAAAAGACGGGAAATTATATCATCATATATTAAATCCTTTTACCGGTTATCCTGC
AGATAATCACTTATATAGCGTTACTATAATAACAGATACTTCTATAGATGGTGATGCACTAGCAAAAATTTTTGTTATGG
GTTTAGATGAGGGTCTGAAGGTTGCAGAAAATTTACCTGGAGTAGAAGCAATATTTGTGACAGATGATAAAAAAGTGTAT
ATAACTTCTGGGTTAAAAGGAAATTTTAGGATTACCGATCCAGAATATACTTTAATGACTAAGTAA

Upstream 100 bases:

>100_bases
GATTCTCCACCAAAACAAATTTTAATTGATGTGACGACTAAGCTTTTATATAGAAATTACTAAAATTGGTTAAAGGCCTT
ATTTTTGGAGGAGTTATTAT

Downstream 100 bases:

>100_bases
TTCCATTTTTCTGGAGGAAGTTTTATGGATAAAAAGGAATTTTTAAGGGTATGGAAGGGTTTTTGGCAGTTGGCAGACCC
TAAGATATGGATAGCATCTA

Product: thiamine biosynthesis lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKKFIVLFSIFLLIFIFSGCSNKNSQPISDTKFALDTYCTLTVYDKVPKKVLDDGFKAIEDIEKKMSVTIEDSEVSEINK
NAGIRPVKVSPDTFYVIKKAIHFSEIEKGYFDITVGPIVNLWNIGTDKARVPSAEEIKAKLPLVNYKNIVLDEKNMTVML
KDKGMSIDLGGIAKGYAADKVAEVLKKEGVKHAIINLGGDVLTIGTKPDGTNWRVGIQTPFKPRGEYFGIVEVGEKAVVT
SGIYERYFEKDGKLYHHILNPFTGYPADNHLYSVTIITDTSIDGDALAKIFVMGLDEGLKVAENLPGVEAIFVTDDKKVY
ITSGLKGNFRITDPEYTLMTK

Sequences:

>Translated_341_residues
MKKFIVLFSIFLLIFIFSGCSNKNSQPISDTKFALDTYCTLTVYDKVPKKVLDDGFKAIEDIEKKMSVTIEDSEVSEINK
NAGIRPVKVSPDTFYVIKKAIHFSEIEKGYFDITVGPIVNLWNIGTDKARVPSAEEIKAKLPLVNYKNIVLDEKNMTVML
KDKGMSIDLGGIAKGYAADKVAEVLKKEGVKHAIINLGGDVLTIGTKPDGTNWRVGIQTPFKPRGEYFGIVEVGEKAVVT
SGIYERYFEKDGKLYHHILNPFTGYPADNHLYSVTIITDTSIDGDALAKIFVMGLDEGLKVAENLPGVEAIFVTDDKKVY
ITSGLKGNFRITDPEYTLMTK
>Mature_341_residues
MKKFIVLFSIFLLIFIFSGCSNKNSQPISDTKFALDTYCTLTVYDKVPKKVLDDGFKAIEDIEKKMSVTIEDSEVSEINK
NAGIRPVKVSPDTFYVIKKAIHFSEIEKGYFDITVGPIVNLWNIGTDKARVPSAEEIKAKLPLVNYKNIVLDEKNMTVML
KDKGMSIDLGGIAKGYAADKVAEVLKKEGVKHAIINLGGDVLTIGTKPDGTNWRVGIQTPFKPRGEYFGIVEVGEKAVVT
SGIYERYFEKDGKLYHHILNPFTGYPADNHLYSVTIITDTSIDGDALAKIFVMGLDEGLKVAENLPGVEAIFVTDDKKVY
ITSGLKGNFRITDPEYTLMTK

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=244, Percent_Identity=29.9180327868852, Blast_Score=135, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 37844; Mature: 37844

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFIVLFSIFLLIFIFSGCSNKNSQPISDTKFALDTYCTLTVYDKVPKKVLDDGFKAIE
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEEEEHHHHHHHHHHHHHHHH
DIEKKMSVTIEDSEVSEINKNAGIRPVKVSPDTFYVIKKAIHFSEIEKGYFDITVGPIVN
HHHHHHEEEEECCHHHHHHHCCCCEEEEECCCCEEHHHHHHHHHHHCCCEEEEEECCEEE
LWNIGTDKARVPSAEEIKAKLPLVNYKNIVLDEKNMTVMLKDKGMSIDLGGIAKGYAADK
EECCCCCCCCCCCHHHHHHHCCEECCCEEEEECCCCEEEEECCCCEEECCCCCCCHHHHH
VAEVLKKEGVKHAIINLGGDVLTIGTKPDGTNWRVGIQTPFKPRGEYFGIVEVGEKAVVT
HHHHHHHCCCEEEEEECCCCEEEECCCCCCCCEEEEECCCCCCCCCEEEEEECCCCHHHH
SGIYERYFEKDGKLYHHILNPFTGYPADNHLYSVTIITDTSIDGDALAKIFVMGLDEGLK
HHHHHHHHHCCCHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCHHHHHEEHEEECCCCHH
VAENLPGVEAIFVTDDKKVYITSGLKGNFRITDPEYTLMTK
HHHCCCCCEEEEEECCCEEEEECCCCCCEEEECCCEEEEEC
>Mature Secondary Structure
MKKFIVLFSIFLLIFIFSGCSNKNSQPISDTKFALDTYCTLTVYDKVPKKVLDDGFKAIE
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEEEEHHHHHHHHHHHHHHHH
DIEKKMSVTIEDSEVSEINKNAGIRPVKVSPDTFYVIKKAIHFSEIEKGYFDITVGPIVN
HHHHHHEEEEECCHHHHHHHCCCCEEEEECCCCEEHHHHHHHHHHHCCCEEEEEECCEEE
LWNIGTDKARVPSAEEIKAKLPLVNYKNIVLDEKNMTVMLKDKGMSIDLGGIAKGYAADK
EECCCCCCCCCCCHHHHHHHCCEECCCEEEEECCCCEEEEECCCCEEECCCCCCCHHHHH
VAEVLKKEGVKHAIINLGGDVLTIGTKPDGTNWRVGIQTPFKPRGEYFGIVEVGEKAVVT
HHHHHHHCCCEEEEEECCCCEEEECCCCCCCCEEEEECCCCCCCCCEEEEEECCCCHHHH
SGIYERYFEKDGKLYHHILNPFTGYPADNHLYSVTIITDTSIDGDALAKIFVMGLDEGLK
HHHHHHHHHCCCHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCHHHHHEEHEEECCCCHH
VAENLPGVEAIFVTDDKKVYITSGLKGNFRITDPEYTLMTK
HHHCCCCCEEEEEECCCEEEEECCCCCCEEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]