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The map label for this gene is MelB2 [H]

Identifier: 20808815

GI number: 20808815

Start: 2345818

End: 2347122

Strand: Reverse

Name: MelB2 [H]

Synonym: TTE2458

Alternate gene names: 20808815

Gene position: 2347122-2345818 (Counterclockwise)

Preceding gene: 20808816

Following gene: 20808814

Centisome position: 87.27

GC content: 39.69

Gene sequence:

>1305_bases
ATGAAGGGAAGAAGGTTTTTCGTGCTTTTTATGTATTCTTTGGGGTACATGGGAATATCAATTTTCACTCAGACGGCGGT
GAAATGGTATCAATACTATTACACTCCTCCTGCTTTCAACACACAGGGGCTTAAAATTCTTGTGCCTATAGGTCTTATAG
GCTTTTCCATGATAGTTGCTCGAATTTTTGATGGGGTTTCTGATCCGATAGTCGCCTACTATTCTGACAAATTGAGGTCT
AGATGGGGAAGAAGGATTCCTTTTGTGCTCTTTGGAAGCCCTGTTTTGTTTTTGTCCTTTATAATGATCTGGTTTCCTCC
TGTAGAGCATGAATCTATATTAAATTTTGTTTATCTGACCTTTGTCCTTAGCTTGTTTTTTGTGGCTTTCACGGCAGTGG
TAGCTCCATATCTTGCTCTTATTCCCGAGATAACAAAAAGCGCAGGGGAAAGAATCACTCTTACGATGATGCAGGGGATA
ACCCAGATTTTGGGCGTCATGGTGGCAGAAGCTGGTTCAGGAATTTTAATTAGGATTTTTAACTTTAAGGTTATGGGAAT
TATACTAGGAAGTTTTGCCTTTGTAACGCTTATGCTGACTCCTTTGTTTGTGAAGGAAGAACCAGTAAAAGAAGAGGATA
TGCCGACAGTCTCGATGTTTGCCTCTATAAAGATGACTTTTTCAAATAAAGACTTCATCTATTACTTGACTGCTATTACG
GCATTGTGGTTTGGGCTTAATACCCTTACAATTGCTATGCCTTATATTACGGAAGTTCTTCTGAAGATTCCTGCTGAAAA
TTCTGGATTTATGATTGCGGGAGCCTTTGTGGTAGCTGTTTTCTTCAGTTTCTTTGTGCCAAAACTAAGCCTTGCATATG
GGAAAAAGAAGGTCTTTATGGTGTTTTCAGCATTTTTTGCTCTGATACTAGGGCTTACAGGCCTTTTTGGCACGGTTTTT
AGTAAAACTTTGTCTATTGTGATAATTTTGCTTGCAGGTATTCCTCTTTCTGTGTTTTTTGTGATACCCAATGCCATGAT
TGCAGACATAGCGGAGTTAGATGGCATTAAAGTTGGGCAGAGAAGAGAAGGTATGTTTTTTGGAGCGCAGGGATTTGTGG
TAAAGGTGGTCATGGGGATTTCGTCACTTGTTACACCTCTTCTCTTTAGAGCCTTTGGATATAGCGCAGAAAGTCCCTTA
GGGCTTATGCTTTGTGGGCCTTTGGCAGGGGTGTTGATACTTTTAAGCTTAGTAGTGTTTAGGAATTATTCCATATCAGA
AAAGGAATTAGAGAAGAAAGGTTAA

Upstream 100 bases:

>100_bases
AAGCGAGGAAAAAAGCAGAGTATTTTGGAGATAGAAATGATGCCTTTGAAAGGGACTATTATGAAAAAGTGTATAAACCT
TTATTGAAAGGAGAAAGAGG

Downstream 100 bases:

>100_bases
GCCTTCTTTGGTGATAACAGATTCTCAAGTTTTTAAAAAAGTGGGAGCCTGTGTTCCGGAAGATGTGCCTCTTACTTCTT
TTTCAATACTCTTTGCTCGG

Product: Na+/melibiose symporter and related transporters

Products: Li (I) [Cytoplasm]; melibiose [Cytoplasm]; Na (I) [Cytoplasm]; melibiose [Cytoplasm]; Proton [Cytoplasm]; melibiose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MKGRRFFVLFMYSLGYMGISIFTQTAVKWYQYYYTPPAFNTQGLKILVPIGLIGFSMIVARIFDGVSDPIVAYYSDKLRS
RWGRRIPFVLFGSPVLFLSFIMIWFPPVEHESILNFVYLTFVLSLFFVAFTAVVAPYLALIPEITKSAGERITLTMMQGI
TQILGVMVAEAGSGILIRIFNFKVMGIILGSFAFVTLMLTPLFVKEEPVKEEDMPTVSMFASIKMTFSNKDFIYYLTAIT
ALWFGLNTLTIAMPYITEVLLKIPAENSGFMIAGAFVVAVFFSFFVPKLSLAYGKKKVFMVFSAFFALILGLTGLFGTVF
SKTLSIVIILLAGIPLSVFFVIPNAMIADIAELDGIKVGQRREGMFFGAQGFVVKVVMGISSLVTPLLFRAFGYSAESPL
GLMLCGPLAGVLILLSLVVFRNYSISEKELEKKG

Sequences:

>Translated_434_residues
MKGRRFFVLFMYSLGYMGISIFTQTAVKWYQYYYTPPAFNTQGLKILVPIGLIGFSMIVARIFDGVSDPIVAYYSDKLRS
RWGRRIPFVLFGSPVLFLSFIMIWFPPVEHESILNFVYLTFVLSLFFVAFTAVVAPYLALIPEITKSAGERITLTMMQGI
TQILGVMVAEAGSGILIRIFNFKVMGIILGSFAFVTLMLTPLFVKEEPVKEEDMPTVSMFASIKMTFSNKDFIYYLTAIT
ALWFGLNTLTIAMPYITEVLLKIPAENSGFMIAGAFVVAVFFSFFVPKLSLAYGKKKVFMVFSAFFALILGLTGLFGTVF
SKTLSIVIILLAGIPLSVFFVIPNAMIADIAELDGIKVGQRREGMFFGAQGFVVKVVMGISSLVTPLLFRAFGYSAESPL
GLMLCGPLAGVLILLSLVVFRNYSISEKELEKKG
>Mature_434_residues
MKGRRFFVLFMYSLGYMGISIFTQTAVKWYQYYYTPPAFNTQGLKILVPIGLIGFSMIVARIFDGVSDPIVAYYSDKLRS
RWGRRIPFVLFGSPVLFLSFIMIWFPPVEHESILNFVYLTFVLSLFFVAFTAVVAPYLALIPEITKSAGERITLTMMQGI
TQILGVMVAEAGSGILIRIFNFKVMGIILGSFAFVTLMLTPLFVKEEPVKEEDMPTVSMFASIKMTFSNKDFIYYLTAIT
ALWFGLNTLTIAMPYITEVLLKIPAENSGFMIAGAFVVAVFFSFFVPKLSLAYGKKKVFMVFSAFFALILGLTGLFGTVF
SKTLSIVIILLAGIPLSVFFVIPNAMIADIAELDGIKVGQRREGMFFGAQGFVVKVVMGISSLVTPLLFRAFGYSAESPL
GLMLCGPLAGVLILLSLVVFRNYSISEKELEKKG

Specific function: Probably involved in glucitol uptake [H]

COG id: COG2211

COG function: function code G; Na+/melibiose symporter and related transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:galactoside symporter (TC 2.A.2) family [H]

Homologues:

Organism=Escherichia coli, GI1790561, Length=375, Percent_Identity=28, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001927
- InterPro:   IPR018043 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48194; Mature: 48194

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGRRFFVLFMYSLGYMGISIFTQTAVKWYQYYYTPPAFNTQGLKILVPIGLIGFSMIVA
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEEHHHHHHHHHHHH
RIFDGVSDPIVAYYSDKLRSRWGRRIPFVLFGSPVLFLSFIMIWFPPVEHESILNFVYLT
HHHCCCCCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHH
FVLSLFFVAFTAVVAPYLALIPEITKSAGERITLTMMQGITQILGVMVAEAGSGILIRIF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCEEEEEE
NFKVMGIILGSFAFVTLMLTPLFVKEEPVKEEDMPTVSMFASIKMTFSNKDFIYYLTAIT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEEECCCCHHHHHHHHH
ALWFGLNTLTIAMPYITEVLLKIPAENSGFMIAGAFVVAVFFSFFVPKLSLAYGKKKVFM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VFSAFFALILGLTGLFGTVFSKTLSIVIILLAGIPLSVFFVIPNAMIADIAELDGIKVGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHCCCCCCC
RREGMFFGAQGFVVKVVMGISSLVTPLLFRAFGYSAESPLGLMLCGPLAGVLILLSLVVF
CCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHH
RNYSISEKELEKKG
CCCCCCHHHHHCCC
>Mature Secondary Structure
MKGRRFFVLFMYSLGYMGISIFTQTAVKWYQYYYTPPAFNTQGLKILVPIGLIGFSMIVA
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEEHHHHHHHHHHHH
RIFDGVSDPIVAYYSDKLRSRWGRRIPFVLFGSPVLFLSFIMIWFPPVEHESILNFVYLT
HHHCCCCCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHH
FVLSLFFVAFTAVVAPYLALIPEITKSAGERITLTMMQGITQILGVMVAEAGSGILIRIF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCEEEEEE
NFKVMGIILGSFAFVTLMLTPLFVKEEPVKEEDMPTVSMFASIKMTFSNKDFIYYLTAIT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEEECCCCHHHHHHHHH
ALWFGLNTLTIAMPYITEVLLKIPAENSGFMIAGAFVVAVFFSFFVPKLSLAYGKKKVFM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VFSAFFALILGLTGLFGTVFSKTLSIVIILLAGIPLSVFFVIPNAMIADIAELDGIKVGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHCCCCCCC
RREGMFFGAQGFVVKVVMGISSLVTPLLFRAFGYSAESPLGLMLCGPLAGVLILLSLVVF
CCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHH
RNYSISEKELEKKG
CCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Li (I) [Periplasm]; melibiose [Periplasm]; Na (I) [Periplasm]; melibiose [Periplasm]; Proton [Periplasm]; melibiose [Periplasm] [C]

Specific reaction: Li (I) [Periplasm] + melibiose [Periplasm] = Li (I) [Cytoplasm] + melibiose [Cytoplasm] Na (I) [Periplasm] + melibiose [Periplasm] = Na (I) [Cytoplasm] + melibiose [Cytoplasm] Proton [Periplasm] + melibiose [Periplasm] = Proton [Cytoplasm] + melibiose [Cy

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9455482; 9384377 [H]