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The map label for this gene is VacB2 [H]

Identifier: 20808801

GI number: 20808801

Start: 2334674

End: 2335087

Strand: Reverse

Name: VacB2 [H]

Synonym: TTE2443

Alternate gene names: 20808801

Gene position: 2335087-2334674 (Counterclockwise)

Preceding gene: 20808802

Following gene: 20808800

Centisome position: 86.82

GC content: 35.99

Gene sequence:

>414_bases
ATGCCGTTTGAAGTTGGGGATATTGTCGAAGGAACTGTATTGAACATCACAGACTTTGGAGCTTTCATTAAGCTACCGGA
GGGGAAAACGGGGCTGGTTCACATCTCTGAAGTTGCGAACACATACGTGAAAAACATCAGGGACTTCTTGAAAGAAAACG
ACAGGGTTAAAGTAAAAATTTTGTCCATGGAACCAAATGGAAAAATAAGCCTGTCTATAAAGAAGGCAATGCCGCAAAAA
ACTCCTAAAGAAAAAGATTTTGCATGGCAAAACAGCAGAAACAACAATAACAGTTCTTTTGAAGAGAAATTATTAAAATT
TTTAAAGGATTCTGATGAAAGACAGCAACAGCTTAGAAAGCATTTAGATTCAAAAAGAAGAAATACAAGTTACAGAAGAA
GCAATGGATATTAA

Upstream 100 bases:

>100_bases
TGAGGGGAAATAGAGGTCAAAAAATCCTGTTGAAATATTTTCTGTTTTAATATATAATAATTCCATTAGCAAAAACTTGA
GAGGGAGGATATTTACTTTT

Downstream 100 bases:

>100_bases
CTTCGGGGCCTCCCGAAGATTTTTCATAAGTTGGGGGTTGTTATGAGAGTCTCTGCGATAGATATTGGAACAAATTCTGT
CAGACAATTGATATGCGACG

Product: hypothetical protein

Products: RNAn; a nucleoside diphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 137; Mature: 136

Protein sequence:

>137_residues
MPFEVGDIVEGTVLNITDFGAFIKLPEGKTGLVHISEVANTYVKNIRDFLKENDRVKVKILSMEPNGKISLSIKKAMPQK
TPKEKDFAWQNSRNNNNSSFEEKLLKFLKDSDERQQQLRKHLDSKRRNTSYRRSNGY

Sequences:

>Translated_137_residues
MPFEVGDIVEGTVLNITDFGAFIKLPEGKTGLVHISEVANTYVKNIRDFLKENDRVKVKILSMEPNGKISLSIKKAMPQK
TPKEKDFAWQNSRNNNNSSFEEKLLKFLKDSDERQQQLRKHLDSKRRNTSYRRSNGY
>Mature_136_residues
PFEVGDIVEGTVLNITDFGAFIKLPEGKTGLVHISEVANTYVKNIRDFLKENDRVKVKILSMEPNGKISLSIKKAMPQKT
PKEKDFAWQNSRNNNNSSFEEKLLKFLKDSDERQQQLRKHLDSKRRNTSYRRSNGY

Specific function: Involved In Mrna Degradation. Hydrolyzes Single-Stranded Polyribonucleotides Processively In The 3' To 5' Direction. Involved In The RNA Degradosome, A Multi-Enzyme Complex Important In RNA Processing And Messenger RNA Degradation. [C]

COG id: COG1098

COG function: function code J; Predicted RNA binding protein (contains ribosomal protein S1 domain)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI145693187, Length=76, Percent_Identity=46.0526315789474, Blast_Score=72, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17535281, Length=76, Percent_Identity=50, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00575 S1 [H]

EC number: 2.7.7.8 [C]

Molecular weight: Translated: 15875; Mature: 15744

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFEVGDIVEGTVLNITDFGAFIKLPEGKTGLVHISEVANTYVKNIRDFLKENDRVKVKI
CCCCCCCCCCCCEEEEECCCCEEECCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCEEEEE
LSMEPNGKISLSIKKAMPQKTPKEKDFAWQNSRNNNNSSFEEKLLKFLKDSDERQQQLRK
EEECCCCEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHH
HLDSKRRNTSYRRSNGY
HHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PFEVGDIVEGTVLNITDFGAFIKLPEGKTGLVHISEVANTYVKNIRDFLKENDRVKVKI
CCCCCCCCCCCEEEEECCCCEEECCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCEEEEE
LSMEPNGKISLSIKKAMPQKTPKEKDFAWQNSRNNNNSSFEEKLLKFLKDSDERQQQLRK
EEECCCCEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHH
HLDSKRRNTSYRRSNGY
HHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 4500 [C]

Specific activity: NA

Km value (mM): NA

Substrates: RNAn+1; phosphate [C]

Specific reaction: RNAn+1 + phosphate = RNAn + a nucleoside diphosphate [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8113187; 7584024; 9384377 [H]