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The map label for this gene is ispD

Identifier: 20808688

GI number: 20808688

Start: 2203325

End: 2204008

Strand: Reverse

Name: ispD

Synonym: TTE2322

Alternate gene names: 20808688

Gene position: 2204008-2203325 (Counterclockwise)

Preceding gene: 20808689

Following gene: 20808687

Centisome position: 81.95

GC content: 37.13

Gene sequence:

>684_bases
ATGAATGTGAGCGCTGTAATAGTAGCCGCTGGAAGAAGCACTAGAATGAATAAAAGTTTGAATAAAGTTTATCTTTCAAT
TGCAGGTAAACCAGTGCTTTATTACTCTATTAAGGCATTTGATGAAATTGAATGGATTAAAGAAATAATTGTGGTCACTT
CTCCAGAAGAGACGGAGTATTGTCAGGAGAACGTCTTGGAGAAATTTTATTGGAATAAGCCTTTTAAAATTGTAAAGGGG
GGTGAAGAAAGGCAGTATTCTGTTTACAACGGAATTTCTGTGGTAGATAAAGATTGTGAAATTGTCGCTATACACGACGG
AGCAAGGCCTTTGGTGACTAAAGAGATTATAATGGAAGCTATAAAAGCAGCTTATCTTTACAAGGCTGCAGCTGTTGGAG
TTCCTGTGAAAGACACCATAAAAGTGGCAGATGAAGATAATTTCATTTTAGATACTCCTGACAGGAGATACCTGTGGGCT
ATTCAAACTCCCCAGGTATTTGAAAAAGAATTGATAGTGAAAGCTCACAGGAAAGCTCTTGAAGAGGGCTTTTTGGGTAC
AGATGACAGCGTTTTGGTGGAAAGGATGGGATTTAAAGTTAAACTAGTGGAAGGGGATTATAAAAATATCAAAATAACTA
CTCCAGAAGATTTGGTCGTGGCAGAACTTTTTTTGAGAAAATAA

Upstream 100 bases:

>100_bases
GCAGAACAGGAAAAAGCGATTTGACACAGGGTTTCCTGTGTTTTTTTGTTTAGAAAATGGTATAATTGGTTTGTAGAATA
TTTTATAAGAGGGATAGTAA

Downstream 100 bases:

>100_bases
CCTTTTTTGAGTTTCCTCATATAATTTAGTAGGACTGTTACCTACTAAATTGTATGAGGGTGATATCTATGAATAATCCT
GTGGTTTTTTTACACGGAAT

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MNVSAVIVAAGRSTRMNKSLNKVYLSIAGKPVLYYSIKAFDEIEWIKEIIVVTSPEETEYCQENVLEKFYWNKPFKIVKG
GEERQYSVYNGISVVDKDCEIVAIHDGARPLVTKEIIMEAIKAAYLYKAAAVGVPVKDTIKVADEDNFILDTPDRRYLWA
IQTPQVFEKELIVKAHRKALEEGFLGTDDSVLVERMGFKVKLVEGDYKNIKITTPEDLVVAELFLRK

Sequences:

>Translated_227_residues
MNVSAVIVAAGRSTRMNKSLNKVYLSIAGKPVLYYSIKAFDEIEWIKEIIVVTSPEETEYCQENVLEKFYWNKPFKIVKG
GEERQYSVYNGISVVDKDCEIVAIHDGARPLVTKEIIMEAIKAAYLYKAAAVGVPVKDTIKVADEDNFILDTPDRRYLWA
IQTPQVFEKELIVKAHRKALEEGFLGTDDSVLVERMGFKVKLVEGDYKNIKITTPEDLVVAELFLRK
>Mature_227_residues
MNVSAVIVAAGRSTRMNKSLNKVYLSIAGKPVLYYSIKAFDEIEWIKEIIVVTSPEETEYCQENVLEKFYWNKPFKIVKG
GEERQYSVYNGISVVDKDCEIVAIHDGARPLVTKEIIMEAIKAAYLYKAAAVGVPVKDTIKVADEDNFILDTPDRRYLWA
IQTPQVFEKELIVKAHRKALEEGFLGTDDSVLVERMGFKVKLVEGDYKNIKITTPEDLVVAELFLRK

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=235, Percent_Identity=28.0851063829787, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI157671913, Length=130, Percent_Identity=30, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1789104, Length=230, Percent_Identity=34.7826086956522, Blast_Score=135, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_THETN (Q8R7S6)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623859.1
- ProteinModelPortal:   Q8R7S6
- GeneID:   996496
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE2322
- NMPDR:   fig|273068.3.peg.2500
- HOGENOM:   HBG672839
- OMA:   PSNIKVT
- ProtClustDB:   PRK00155
- BioCyc:   TTEN273068:TTE2322-MONOMER
- BRENDA:   2.7.7.60
- HAMAP:   MF_00108
- InterPro:   IPR001228
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 25800; Mature: 25800

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVSAVIVAAGRSTRMNKSLNKVYLSIAGKPVLYYSIKAFDEIEWIKEIIVVTSPEETEY
CCCEEEEEECCCCCCHHHHHHEEEEEECCCEEEEEEEHHHHHHHHHHHEEEEECCCHHHH
CQENVLEKFYWNKPFKIVKGGEERQYSVYNGISVVDKDCEIVAIHDGARPLVTKEIIMEA
HHHHHHHHHHCCCCEEEEECCCCCEEEEECCCEEECCCCEEEEEECCCCCHHHHHHHHHH
IKAAYLYKAAAVGVPVKDTIKVADEDNFILDTPDRRYLWAIQTPQVFEKELIVKAHRKAL
HHHHHHHHHHHCCCCCHHEEEEECCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHH
EEGFLGTDDSVLVERMGFKVKLVEGDYKNIKITTPEDLVVAELFLRK
HHCCCCCCHHHHHHHCCCEEEEEECCCCEEEEECCCHHHHHHHHHCC
>Mature Secondary Structure
MNVSAVIVAAGRSTRMNKSLNKVYLSIAGKPVLYYSIKAFDEIEWIKEIIVVTSPEETEY
CCCEEEEEECCCCCCHHHHHHEEEEEECCCEEEEEEEHHHHHHHHHHHEEEEECCCHHHH
CQENVLEKFYWNKPFKIVKGGEERQYSVYNGISVVDKDCEIVAIHDGARPLVTKEIIMEA
HHHHHHHHHHCCCCEEEEECCCCCEEEEECCCEEECCCCEEEEEECCCCCHHHHHHHHHH
IKAAYLYKAAAVGVPVKDTIKVADEDNFILDTPDRRYLWAIQTPQVFEKELIVKAHRKAL
HHHHHHHHHHHCCCCCHHEEEEECCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHH
EEGFLGTDDSVLVERMGFKVKLVEGDYKNIKITTPEDLVVAELFLRK
HHCCCCCCHHHHHHHCCCEEEEEECCCCEEEEECCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336