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The map label for this gene is AmiC [H]

Identifier: 20808594

GI number: 20808594

Start: 2125271

End: 2125948

Strand: Reverse

Name: AmiC [H]

Synonym: TTE2217

Alternate gene names: 20808594

Gene position: 2125948-2125271 (Counterclockwise)

Preceding gene: 20808595

Following gene: 20808593

Centisome position: 79.05

GC content: 37.32

Gene sequence:

>678_bases
GTGAGATTAAAAAGATGGCTTTTGTTTGGCTTGTTGTTGCTTGTTTCAGTTTTTTCATATAATTGGCAGACAAAAAATCT
TTCTGTTTTTACTACATTGCCAATAATGAATAAAATAATTGTAATTGACGCTGGACACGGAGGTTATGACCCGGGAAAAC
CGGGGAAATACGGAAAGGATGAAGATGAACTGAATTTAGAGATTGCACAAAAATTGAAAGAACTGATTGAACACACAGGG
GGCATTGCAGTTATGATAAGAGAAGACGATTCTCTGTCGGATTCTTCCCTCTCAAAAGACCTGAAAAACAGGGTTTTAAA
GGCCAATCAGGTGGAGGGGGATGTTTTAATTAGCATACATCAAAATAGCTTTTCCCAGTCGAAATATAAAGGAGCTCAGG
TGTTTTACCAGCAAAATTCTGAAAAGGGGAAGTTGCTAGCGGAATTAATTCAAGAAGAACTCAGGAAAACCCTGGATCCA
AATAATGACAGGATGGCAAAGAGCTCCAACAGTTATTATATTTTAAGAAATGCTAAGATGCCTGCAGTGATTGTAGAATG
CGGATTTATGTCAAATCCTGGAGAAGAAAGGTTGTTAAACGACCCTAATTATCAGTACAAAATAGCGTGGGCTATTTACA
AAGGGATCATAGCTTATTTTCAAAAAACTTCCGAATGA

Upstream 100 bases:

>100_bases
CTACTTGACGCTACCCTAGATTCATAATATGTGAATTTTTTTATTAAGTGACAATAGACTGTTTATAGTGAAACTTTTAT
GAGAGGAAAGGAGGGGCCAT

Downstream 100 bases:

>100_bases
GTGTGTTATCCTCTTGTTACTCTTGTCTTTTTTTTGTATACTAAAGAAAAAGGAATATTTAAAAGGAAGGATGTTATGAA
GATAACTGCAGTGCTTTTTG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MRLKRWLLFGLLLLVSVFSYNWQTKNLSVFTTLPIMNKIIVIDAGHGGYDPGKPGKYGKDEDELNLEIAQKLKELIEHTG
GIAVMIREDDSLSDSSLSKDLKNRVLKANQVEGDVLISIHQNSFSQSKYKGAQVFYQQNSEKGKLLAELIQEELRKTLDP
NNDRMAKSSNSYYILRNAKMPAVIVECGFMSNPGEERLLNDPNYQYKIAWAIYKGIIAYFQKTSE

Sequences:

>Translated_225_residues
MRLKRWLLFGLLLLVSVFSYNWQTKNLSVFTTLPIMNKIIVIDAGHGGYDPGKPGKYGKDEDELNLEIAQKLKELIEHTG
GIAVMIREDDSLSDSSLSKDLKNRVLKANQVEGDVLISIHQNSFSQSKYKGAQVFYQQNSEKGKLLAELIQEELRKTLDP
NNDRMAKSSNSYYILRNAKMPAVIVECGFMSNPGEERLLNDPNYQYKIAWAIYKGIIAYFQKTSE
>Mature_225_residues
MRLKRWLLFGLLLLVSVFSYNWQTKNLSVFTTLPIMNKIIVIDAGHGGYDPGKPGKYGKDEDELNLEIAQKLKELIEHTG
GIAVMIREDDSLSDSSLSKDLKNRVLKANQVEGDVLISIHQNSFSQSKYKGAQVFYQQNSEKGKLLAELIQEELRKTLDP
NNDRMAKSSNSYYILRNAKMPAVIVECGFMSNPGEERLLNDPNYQYKIAWAIYKGIIAYFQKTSE

Specific function: Unknown

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=225, Percent_Identity=28.8888888888889, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR014234 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 25603; Mature: 25603

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLKRWLLFGLLLLVSVFSYNWQTKNLSVFTTLPIMNKIIVIDAGHGGYDPGKPGKYGKD
CCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCC
EDELNLEIAQKLKELIEHTGGIAVMIREDDSLSDSSLSKDLKNRVLKANQVEGDVLISIH
HHHCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEE
QNSFSQSKYKGAQVFYQQNSEKGKLLAELIQEELRKTLDPNNDRMAKSSNSYYILRNAKM
CCCCCHHHHCCHHEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCC
PAVIVECGFMSNPGEERLLNDPNYQYKIAWAIYKGIIAYFQKTSE
CEEEEEECCCCCCCHHHCCCCCCCEEEEHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRLKRWLLFGLLLLVSVFSYNWQTKNLSVFTTLPIMNKIIVIDAGHGGYDPGKPGKYGKD
CCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCC
EDELNLEIAQKLKELIEHTGGIAVMIREDDSLSDSSLSKDLKNRVLKANQVEGDVLISIH
HHHCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEE
QNSFSQSKYKGAQVFYQQNSEKGKLLAELIQEELRKTLDPNNDRMAKSSNSYYILRNAKM
CCCCCHHHHCCHHEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCC
PAVIVECGFMSNPGEERLLNDPNYQYKIAWAIYKGIIAYFQKTSE
CEEEEEECCCCCCCHHHCCCCCCCEEEEHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7559346; 8969501; 9384377 [H]