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The map label for this gene is TrmA2 [C]

Identifier: 20808229

GI number: 20808229

Start: 1758775

End: 1760133

Strand: Reverse

Name: TrmA2 [C]

Synonym: TTE1812

Alternate gene names: 20808229

Gene position: 1760133-1758775 (Counterclockwise)

Preceding gene: 20808230

Following gene: 20808227

Centisome position: 65.45

GC content: 37.6

Gene sequence:

>1359_bases
TTGAATAAAGTAAAAATAGGTGAAAAATACGAAGTAGATATCACTAGCATGGGACACGAGGGGGAGGGAGTTGGAAGAAT
CGATGGAATAGCTGTTTTTGTGAAAGGAGCCTTAAAGGGAGAAAGAGTTATTGTAGAGATAGAAGAGGTCCATAAAAATT
ATCTCAAAGGTTATACAGTAAAAATACTAGAAAAATCCCAGCATAGAGTAAATCCTCTTTGTCAGTATGCTGATAGATGT
GGTGGTTGTTCTTTGCAACACTTAAGTTATAAAGGCCAACTTGAATACAAGACTCAGAAGGTAAAGGATAATCTAGAGAG
GATAGGAAAGATTTATACAAAAGTTCACTATGCGATAGGAATGGAGAATCCATTGAATTACAGAAACAAAGGGCAATTTG
TAGTAGGTGAAATAGGAAGAGAAAAGATAACGGGGTTTTACTCTTTCCATTCCCACGAGATTGTACCTGTAGATAATTGC
TTGATACAGCATCCTTTGAGCAACAAGGTTGTAAGTGTAGTAAAAGAGTGGTTAAATGAATGTGACATAAGCGTTTATGA
CAGGAAAAAAGGCAAAGGACTAATAAGGCACGTGGTAGCTAAGGTCGCTTTTAAGACAGGGGAAGTTATGGCAATAATCG
TCATAAATGGAGAAGATATACCTTGTAAGGAAGAACTGATAGAGGCCTTGAAAGAAAAAGTGCCAGGGCTTAAAAGTGTT
ATTTTGAATATTAATACCAGGCGAACTAAGATGATTTTAGGAAATAAGAATATTGTAATTTATGGGAAAAATACCATTGA
GGATTTCATAAAAGATTTAAGATTTGAGATATCTCCACTTTCTTTTTTTCAGGTAAATCCTATTCAAACTGAAATTTTGT
ACGATAAAGCCATTGAATACGCTGGTTTGACGGGAAAAGAAGTGGTAATAGATGTTTATTCAGGAATAGGGACTATATCT
TTGTTTGCAGCCAAGAAAGCCTCTTTTGTATATGGAATTGAGTCAGTAAAAGAGGCGGTAGAGGATGCAGAGAAAAATGC
TCAAGTGAACGGAGTGAAAAATGTGAAGTTTATATGGGGGAAAGCGGAAAGAGAGATAGCCAAACTTTACAAAGAAGGAG
TAAAGGCGGAAGTGGTCATAGTTGACCCGCCAAGAGATGGATGCGATAAGGAGGTTATAAGGGCTATTGTGGGTATAAAC
CCGAAAAGGATCGTGTACGTATCCTGCAATCCTTCTACTCTTGCCAGAGATTTAAGGTATTTAGAAGATGCGGGCTACAG
GACAGTAGAGGTGCAGCCTGTTGACATGTTTCCCTATACTTATCATGTGGAAAGCGTGGCCTTAGTAGAAAGAATGTAG

Upstream 100 bases:

>100_bases
ATTGTATACAAAAGGTATGTATTAGGGGAGAGATAAAACTCCCCCTATATTTTTTCTTTTTGCGATAGGTTATAATACTC
TTTGGAGGAGGGAAGAAACC

Downstream 100 bases:

>100_bases
AAAATATATCAAACTTTAAAAGAAGTCGAAAAAAGTTTAAAAGAGCGGTAAAGGGGAGTTACCCTTTTACTGCTCTTTTT
AGTTTGTTTATCTCTCTCTT

Product: SAM-dependent methyltransferase related to tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MNKVKIGEKYEVDITSMGHEGEGVGRIDGIAVFVKGALKGERVIVEIEEVHKNYLKGYTVKILEKSQHRVNPLCQYADRC
GGCSLQHLSYKGQLEYKTQKVKDNLERIGKIYTKVHYAIGMENPLNYRNKGQFVVGEIGREKITGFYSFHSHEIVPVDNC
LIQHPLSNKVVSVVKEWLNECDISVYDRKKGKGLIRHVVAKVAFKTGEVMAIIVINGEDIPCKEELIEALKEKVPGLKSV
ILNINTRRTKMILGNKNIVIYGKNTIEDFIKDLRFEISPLSFFQVNPIQTEILYDKAIEYAGLTGKEVVIDVYSGIGTIS
LFAAKKASFVYGIESVKEAVEDAEKNAQVNGVKNVKFIWGKAEREIAKLYKEGVKAEVVIVDPPRDGCDKEVIRAIVGIN
PKRIVYVSCNPSTLARDLRYLEDAGYRTVEVQPVDMFPYTYHVESVALVERM

Sequences:

>Translated_452_residues
MNKVKIGEKYEVDITSMGHEGEGVGRIDGIAVFVKGALKGERVIVEIEEVHKNYLKGYTVKILEKSQHRVNPLCQYADRC
GGCSLQHLSYKGQLEYKTQKVKDNLERIGKIYTKVHYAIGMENPLNYRNKGQFVVGEIGREKITGFYSFHSHEIVPVDNC
LIQHPLSNKVVSVVKEWLNECDISVYDRKKGKGLIRHVVAKVAFKTGEVMAIIVINGEDIPCKEELIEALKEKVPGLKSV
ILNINTRRTKMILGNKNIVIYGKNTIEDFIKDLRFEISPLSFFQVNPIQTEILYDKAIEYAGLTGKEVVIDVYSGIGTIS
LFAAKKASFVYGIESVKEAVEDAEKNAQVNGVKNVKFIWGKAEREIAKLYKEGVKAEVVIVDPPRDGCDKEVIRAIVGIN
PKRIVYVSCNPSTLARDLRYLEDAGYRTVEVQPVDMFPYTYHVESVALVERM
>Mature_452_residues
MNKVKIGEKYEVDITSMGHEGEGVGRIDGIAVFVKGALKGERVIVEIEEVHKNYLKGYTVKILEKSQHRVNPLCQYADRC
GGCSLQHLSYKGQLEYKTQKVKDNLERIGKIYTKVHYAIGMENPLNYRNKGQFVVGEIGREKITGFYSFHSHEIVPVDNC
LIQHPLSNKVVSVVKEWLNECDISVYDRKKGKGLIRHVVAKVAFKTGEVMAIIVINGEDIPCKEELIEALKEKVPGLKSV
ILNINTRRTKMILGNKNIVIYGKNTIEDFIKDLRFEISPLSFFQVNPIQTEILYDKAIEYAGLTGKEVVIDVYSGIGTIS
LFAAKKASFVYGIESVKEAVEDAEKNAQVNGVKNVKFIWGKAEREIAKLYKEGVKAEVVIVDPPRDGCDKEVIRAIVGIN
PKRIVYVSCNPSTLARDLRYLEDAGYRTVEVQPVDMFPYTYHVESVALVERM

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI34222389, Length=353, Percent_Identity=32.2946175637394, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI51173878, Length=353, Percent_Identity=32.2946175637394, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI269784678, Length=417, Percent_Identity=27.3381294964029, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI269784680, Length=357, Percent_Identity=28.2913165266106, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI269784676, Length=357, Percent_Identity=28.2913165266106, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI20149688, Length=357, Percent_Identity=28.2913165266106, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1789148, Length=446, Percent_Identity=28.47533632287, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI1787083, Length=377, Percent_Identity=25.7294429708223, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1790403, Length=153, Percent_Identity=32.6797385620915, Blast_Score=79, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71997438, Length=205, Percent_Identity=32.6829268292683, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6322909, Length=158, Percent_Identity=31.6455696202532, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24666579, Length=417, Percent_Identity=30.9352517985612, Blast_Score=153, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1812_THETN (Q8R918)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623400.1
- ProteinModelPortal:   Q8R918
- SMR:   Q8R918
- GeneID:   996993
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1812
- NMPDR:   fig|273068.3.peg.1550
- HOGENOM:   HBG690163
- OMA:   RKGCDEA
- ProtClustDB:   CLSK901425
- BioCyc:   TTEN273068:TTE1812-MONOMER
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: NA

Molecular weight: Translated: 50937; Mature: 50937

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS50926 TRAM; PS01230 TRMA_1; PS01231 TRMA_2; PS00059 ADH_ZINC

Important sites: ACT_SITE 409-409 BINDING 284-284 BINDING 313-313 BINDING 334-334 BINDING 382-382

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVKIGEKYEVDITSMGHEGEGVGRIDGIAVFVKGALKGERVIVEIEEVHKNYLKGYTV
CCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCEEEEEHHHHHHHHHCCCEE
KILEKSQHRVNPLCQYADRCGGCSLQHLSYKGQLEYKTQKVKDNLERIGKIYTKVHYAIG
EEEECCCHHCCHHHHHHHHCCCCCEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHEEEC
MENPLNYRNKGQFVVGEIGREKITGFYSFHSHEIVPVDNCLIQHPLSNKVVSVVKEWLNE
CCCCCCCCCCCCEEEEECCCHHHEEEEEECCCCEEEHHHHHCCCCCCHHHHHHHHHHHHH
CDISVYDRKKGKGLIRHVVAKVAFKTGEVMAIIVINGEDIPCKEELIEALKEKVPGLKSV
CCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEE
ILNINTRRTKMILGNKNIVIYGKNTIEDFIKDLRFEISPLSFFQVNPIQTEILYDKAIEY
EEECCCCEEEEEECCCEEEEEECHHHHHHHHHHHEECCCEEEEEECCCHHHHHHHHHHHH
AGLTGKEVVIDVYSGIGTISLFAAKKASFVYGIESVKEAVEDAEKNAQVNGVKNVKFIWG
CCCCCCCEEEEECCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCEEEEEC
KAEREIAKLYKEGVKAEVVIVDPPRDGCDKEVIRAIVGINPKRIVYVSCNPSTLARDLRY
CHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHH
LEDAGYRTVEVQPVDMFPYTYHVESVALVERM
HHHCCCEEEEEEEEEECCEEEEHHHHHHHHCC
>Mature Secondary Structure
MNKVKIGEKYEVDITSMGHEGEGVGRIDGIAVFVKGALKGERVIVEIEEVHKNYLKGYTV
CCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCEEEEEHHHHHHHHHCCCEE
KILEKSQHRVNPLCQYADRCGGCSLQHLSYKGQLEYKTQKVKDNLERIGKIYTKVHYAIG
EEEECCCHHCCHHHHHHHHCCCCCEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHEEEC
MENPLNYRNKGQFVVGEIGREKITGFYSFHSHEIVPVDNCLIQHPLSNKVVSVVKEWLNE
CCCCCCCCCCCCEEEEECCCHHHEEEEEECCCCEEEHHHHHCCCCCCHHHHHHHHHHHHH
CDISVYDRKKGKGLIRHVVAKVAFKTGEVMAIIVINGEDIPCKEELIEALKEKVPGLKSV
CCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEE
ILNINTRRTKMILGNKNIVIYGKNTIEDFIKDLRFEISPLSFFQVNPIQTEILYDKAIEY
EEECCCCEEEEEECCCEEEEEECHHHHHHHHHHHEECCCEEEEEECCCHHHHHHHHHHHH
AGLTGKEVVIDVYSGIGTISLFAAKKASFVYGIESVKEAVEDAEKNAQVNGVKNVKFIWG
CCCCCCCEEEEECCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCEEEEEC
KAEREIAKLYKEGVKAEVVIVDPPRDGCDKEVIRAIVGINPKRIVYVSCNPSTLARDLRY
CHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHH
LEDAGYRTVEVQPVDMFPYTYHVESVALVERM
HHHCCCEEEEEEEEEECCEEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336