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The map label for this gene is MetH2 [H]

Identifier: 20808220

GI number: 20808220

Start: 1746876

End: 1749287

Strand: Reverse

Name: MetH2 [H]

Synonym: TTE1803

Alternate gene names: 20808220

Gene position: 1749287-1746876 (Counterclockwise)

Preceding gene: 20808221

Following gene: 20808219

Centisome position: 65.04

GC content: 40.55

Gene sequence:

>2412_bases
GTGGTAGATATTTTTAAAGAATGTGAAAATAGGGTTGTCGTTTTTGACGGTGCTATGGGGACACAGCTTCAGGAAAGGGG
ACTTAAAGCCGGGGAATGCCCTGAGTATATAAACTTAAAAATGCCTGAGGTGGTTTTTGACATACACAAAGCCTATATTG
AGGCTGGAGCCGAAGTCATAGAGACTAATACTTTTGGGGCAAACAGAATAAAACTTGCAAAATACGGACTTGAGGACAAA
GTAGAAGAGATTGTAACGAAAGGGGTGGAAATAGCGAGAAAGGCGGCGGGAGATCGTCCAGTAGCCCTCTCTGTTGGCCC
AACAGGTGAACTTCTTGCTCCATTTGGAGATATGACTTTTGATGAGGCTTATGAGGTTTTCAAGGAAGTGGTAGTAGCCG
CTGAAAAAGCAGGAGCAGACATTGTGATCATAGAAACCATGTCAGATATGTTGGAGGCAAAAGCTGCAATTTTAGCAGCT
AAGGAAAACACCAATATGAAGGTCATATGTACCATGACTTTTCAGGAAGACGGACGAACACTTATGGGTTCGGACCCTGT
GACGGTAGTAGTAAGTCTACAGGGTTTGGGACTTGATGCAATAGGAGTTAACTGTTCTACAGGGCCGGATAAAATGGTAA
AAGTTGTTGAAAAGATGGCTCAAGTGGCGCGTATTCCCATAATTGCTCAGCCGAATGCCGGTATGCCTGTCATAAGGGAC
GGGAAAACAGTTTACGATTTAAAACCTGAGGAATTTGCCAGTTTCTTTCCACAACTTGTTGAAAAAGGCGCTTGTATAGT
AGGAGGTTGTTGCGGTACTACTCCATACTATATAAAACTGGTAAAAAAGGCTGTAGAAAATTTAAAGCCTAAAAAGCTGG
AAAATAAATTTACAGCTCTGTCTTCAAATACCCGCACAGTTTTTATAGGCAAAGAATACCCTTTAAGGGTGATAGGAGAG
AGGATTAATCCTACAGGAAAGAAAAAATTAAGCGGAGCTCTTTTGTCAGGAGATGTCAATTTAGCTGTTGAGGAAGCTTT
GAAGCAGCAAAAGTGCGGGGCCGAAATATTAGATGTCAATGTAGGGGTGCCTGGGATAGAGGAAGAGGAAATGTTGCCAA
AGGTAGCGTTTGAAGTTGAAAAGGCGGTGGATTTGCCTTTGCAGTTAGATAGTACCAATGTAAAAGCTATAGAGAAAGCG
ATAAGAATTTTAAGAGGAAGGCCTATAATAAATTCTGTTAATGCAAAGGAAGAGAGCTTAAAAGAAGTATTGCCAATAGT
TAAAAAGTACGGAGCATGTGTAGTTGGGCTTACAGTGGGGGATAAGGGATTGCCAAAGGACAGACATGAGAGGGTTGAAA
ATGCCAGAAAAATACTGAAGAAAGCAGAAGAGTACGGAATTCCTAAGGAAGATGTGATAATAGACCCCGTGGTGCTTACT
GTTTCCTCAGAACAAGGAGCAGCAATTGAAACATTAGAAGCGATGAAAATAATATCAGAGGAATTGGGTGTAAACACGGT
TGTAGGGCTAAGCAATATATCTTTTGGTCTGCCTGAAAGAAAGCTCATAAATTCGACTTTCTTGGCCATGGCAGCTTCAC
ACGGGCTTACTGCGGCTATTGTAAATCCCTGCGATGAATCCTTGATGGACACTTTGAGGGCTTCTATGGTTTTGCTTAAC
AAAGATAAAGGAAGTGAAAATTATTTAAAAGTGTATGGGCAAAGGGTAAAGCCGAAAGAAGAAGTCATAAAAAGACATGA
GGAAGATTTTAGCAAAAAGCTGTATGATCAGATTTTAGAAGGGAAAAAGTCGGGAGTTGAAGAAATAGTAATGGCACTCC
TTGAGGATAACCCTCCCCTTTCACTAGTGGATGAAATTATAATACCTGCGCTTAAAGAAGTAGGGGACAGGTATGAAAAA
GGTATATACTTTTTGCCACAGCTTTTGAGTTCTGCGGAGGTCGTGCAGAGTGCTTTTAAATTGATAAAGGAAAAGCTTCC
AAAAGGCACTGCTTCAAAAGGGAAAATAATTTTAGCAACAGTGGAGGGAGATGTACACGATATAGGGAAAAACATAGTTA
AGGTTTTGTTAGAGAATTACGGGTATGAGGTAATTGACCTTGGCAAAGACGTAAAAGGCGAGGTAATAGTGGAGGAAGTG
AAAAAGACAGGTGCTCCTCTGGTAGGGTTGAGTGCTCTTATGACTACTACTCTTTTTAATATGGAAAAAATTATTAAAAT
GCTTAAAGAGAATACTGAAGCGAAAGTGATGGTTGGTGGAGCAGTTTTAACAGAAGAGTACGCTTTTAAAATAGGAGCGG
ATTACTACGGTAAGACCGCACAGGATGCTGTAAAAATTGCTGATAAATTTTTCTTAAAGAACGCTTTGGTTTGTAAGACC
TGCAGTTTTTAA

Upstream 100 bases:

>100_bases
CCCATGGGAAGTGTAGACTTTGTTTTTGATATAATCTCTAAAGTGGAAGGAATAGCTATAAGTGCTAAAAAGGCTGAGAC
GAAGTAATGGGGGGATGAAA

Downstream 100 bases:

>100_bases
AATACGCATATTGAGGTGATGAAATTGTCAGTAGAGGCGGTTAGAGATTTTTTTAGGTCAAGAGGGATAAATTTGGAGGT
AAAAATTTTTGAAGATACCA

Product: methionine synthase I cobalamin-binding subunit

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 803; Mature: 803

Protein sequence:

>803_residues
MVDIFKECENRVVVFDGAMGTQLQERGLKAGECPEYINLKMPEVVFDIHKAYIEAGAEVIETNTFGANRIKLAKYGLEDK
VEEIVTKGVEIARKAAGDRPVALSVGPTGELLAPFGDMTFDEAYEVFKEVVVAAEKAGADIVIIETMSDMLEAKAAILAA
KENTNMKVICTMTFQEDGRTLMGSDPVTVVVSLQGLGLDAIGVNCSTGPDKMVKVVEKMAQVARIPIIAQPNAGMPVIRD
GKTVYDLKPEEFASFFPQLVEKGACIVGGCCGTTPYYIKLVKKAVENLKPKKLENKFTALSSNTRTVFIGKEYPLRVIGE
RINPTGKKKLSGALLSGDVNLAVEEALKQQKCGAEILDVNVGVPGIEEEEMLPKVAFEVEKAVDLPLQLDSTNVKAIEKA
IRILRGRPIINSVNAKEESLKEVLPIVKKYGACVVGLTVGDKGLPKDRHERVENARKILKKAEEYGIPKEDVIIDPVVLT
VSSEQGAAIETLEAMKIISEELGVNTVVGLSNISFGLPERKLINSTFLAMAASHGLTAAIVNPCDESLMDTLRASMVLLN
KDKGSENYLKVYGQRVKPKEEVIKRHEEDFSKKLYDQILEGKKSGVEEIVMALLEDNPPLSLVDEIIIPALKEVGDRYEK
GIYFLPQLLSSAEVVQSAFKLIKEKLPKGTASKGKIILATVEGDVHDIGKNIVKVLLENYGYEVIDLGKDVKGEVIVEEV
KKTGAPLVGLSALMTTTLFNMEKIIKMLKENTEAKVMVGGAVLTEEYAFKIGADYYGKTAQDAVKIADKFFLKNALVCKT
CSF

Sequences:

>Translated_803_residues
MVDIFKECENRVVVFDGAMGTQLQERGLKAGECPEYINLKMPEVVFDIHKAYIEAGAEVIETNTFGANRIKLAKYGLEDK
VEEIVTKGVEIARKAAGDRPVALSVGPTGELLAPFGDMTFDEAYEVFKEVVVAAEKAGADIVIIETMSDMLEAKAAILAA
KENTNMKVICTMTFQEDGRTLMGSDPVTVVVSLQGLGLDAIGVNCSTGPDKMVKVVEKMAQVARIPIIAQPNAGMPVIRD
GKTVYDLKPEEFASFFPQLVEKGACIVGGCCGTTPYYIKLVKKAVENLKPKKLENKFTALSSNTRTVFIGKEYPLRVIGE
RINPTGKKKLSGALLSGDVNLAVEEALKQQKCGAEILDVNVGVPGIEEEEMLPKVAFEVEKAVDLPLQLDSTNVKAIEKA
IRILRGRPIINSVNAKEESLKEVLPIVKKYGACVVGLTVGDKGLPKDRHERVENARKILKKAEEYGIPKEDVIIDPVVLT
VSSEQGAAIETLEAMKIISEELGVNTVVGLSNISFGLPERKLINSTFLAMAASHGLTAAIVNPCDESLMDTLRASMVLLN
KDKGSENYLKVYGQRVKPKEEVIKRHEEDFSKKLYDQILEGKKSGVEEIVMALLEDNPPLSLVDEIIIPALKEVGDRYEK
GIYFLPQLLSSAEVVQSAFKLIKEKLPKGTASKGKIILATVEGDVHDIGKNIVKVLLENYGYEVIDLGKDVKGEVIVEEV
KKTGAPLVGLSALMTTTLFNMEKIIKMLKENTEAKVMVGGAVLTEEYAFKIGADYYGKTAQDAVKIADKFFLKNALVCKT
CSF
>Mature_803_residues
MVDIFKECENRVVVFDGAMGTQLQERGLKAGECPEYINLKMPEVVFDIHKAYIEAGAEVIETNTFGANRIKLAKYGLEDK
VEEIVTKGVEIARKAAGDRPVALSVGPTGELLAPFGDMTFDEAYEVFKEVVVAAEKAGADIVIIETMSDMLEAKAAILAA
KENTNMKVICTMTFQEDGRTLMGSDPVTVVVSLQGLGLDAIGVNCSTGPDKMVKVVEKMAQVARIPIIAQPNAGMPVIRD
GKTVYDLKPEEFASFFPQLVEKGACIVGGCCGTTPYYIKLVKKAVENLKPKKLENKFTALSSNTRTVFIGKEYPLRVIGE
RINPTGKKKLSGALLSGDVNLAVEEALKQQKCGAEILDVNVGVPGIEEEEMLPKVAFEVEKAVDLPLQLDSTNVKAIEKA
IRILRGRPIINSVNAKEESLKEVLPIVKKYGACVVGLTVGDKGLPKDRHERVENARKILKKAEEYGIPKEDVIIDPVVLT
VSSEQGAAIETLEAMKIISEELGVNTVVGLSNISFGLPERKLINSTFLAMAASHGLTAAIVNPCDESLMDTLRASMVLLN
KDKGSENYLKVYGQRVKPKEEVIKRHEEDFSKKLYDQILEGKKSGVEEIVMALLEDNPPLSLVDEIIIPALKEVGDRYEK
GIYFLPQLLSSAEVVQSAFKLIKEKLPKGTASKGKIILATVEGDVHDIGKNIVKVLLENYGYEVIDLGKDVKGEVIVEEV
KKTGAPLVGLSALMTTTLFNMEKIIKMLKENTEAKVMVGGAVLTEEYAFKIGADYYGKTAQDAVKIADKFFLKNALVCKT
CSF

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=864, Percent_Identity=29.6296296296296, Blast_Score=288, Evalue=2e-77,
Organism=Homo sapiens, GI157266337, Length=308, Percent_Identity=29.2207792207792, Blast_Score=77, Evalue=7e-14,
Organism=Homo sapiens, GI13162290, Length=303, Percent_Identity=26.0726072607261, Blast_Score=73, Evalue=1e-12,
Organism=Escherichia coli, GI1790450, Length=843, Percent_Identity=30.8422301304864, Blast_Score=341, Evalue=1e-94,
Organism=Escherichia coli, GI1786456, Length=296, Percent_Identity=29.3918918918919, Blast_Score=101, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17535405, Length=855, Percent_Identity=29.7076023391813, Blast_Score=292, Evalue=6e-79,
Organism=Drosophila melanogaster, GI24585077, Length=293, Percent_Identity=26.2798634812287, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 87513; Mature: 87513

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS50970 HCY ; PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDIFKECENRVVVFDGAMGTQLQERGLKAGECPEYINLKMPEVVFDIHKAYIEAGAEVI
CCCCHHHCCCCEEEEECCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEE
ETNTFGANRIKLAKYGLEDKVEEIVTKGVEIARKAAGDRPVALSVGPTGELLAPFGDMTF
ECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHCCCCCCCH
DEAYEVFKEVVVAAEKAGADIVIIETMSDMLEAKAAILAAKENTNMKVICTMTFQEDGRT
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHEEEECCCCEEEEEEEEECCCCCE
LMGSDPVTVVVSLQGLGLDAIGVNCSTGPDKMVKVVEKMAQVARIPIIAQPNAGMPVIRD
EECCCCEEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCEEEC
GKTVYDLKPEEFASFFPQLVEKGACIVGGCCGTTPYYIKLVKKAVENLKPKKLENKFTAL
CCEEEECCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHC
SSNTRTVFIGKEYPLRVIGERINPTGKKKLSGALLSGDVNLAVEEALKQQKCGAEILDVN
CCCCEEEEEECCCCHHHHHCCCCCCCHHHHCCEEECCCCHHHHHHHHHHHHCCCEEEEEE
VGVPGIEEEEMLPKVAFEVEKAVDLPLQLDSTNVKAIEKAIRILRGRPIINSVNAKEESL
CCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCCHHHH
KEVLPIVKKYGACVVGLTVGDKGLPKDRHERVENARKILKKAEEYGIPKEDVIIDPVVLT
HHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCEEEE
VSSEQGAAIETLEAMKIISEELGVNTVVGLSNISFGLPERKLINSTFLAMAASHGLTAAI
EECCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEE
VNPCDESLMDTLRASMVLLNKDKGSENYLKVYGQRVKPKEEVIKRHEEDFSKKLYDQILE
ECCCHHHHHHHHHHHEEEEECCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHC
GKKSGVEEIVMALLEDNPPLSLVDEIIIPALKEVGDRYEKGIYFLPQLLSSAEVVQSAFK
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LIKEKLPKGTASKGKIILATVEGDVHDIGKNIVKVLLENYGYEVIDLGKDVKGEVIVEEV
HHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHH
KKTGAPLVGLSALMTTTLFNMEKIIKMLKENTEAKVMVGGAVLTEEYAFKIGADYYGKTA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHEECCCCCCCCH
QDAVKIADKFFLKNALVCKTCSF
HHHHHHHHHHHHHCCHHHHCCCC
>Mature Secondary Structure
MVDIFKECENRVVVFDGAMGTQLQERGLKAGECPEYINLKMPEVVFDIHKAYIEAGAEVI
CCCCHHHCCCCEEEEECCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEE
ETNTFGANRIKLAKYGLEDKVEEIVTKGVEIARKAAGDRPVALSVGPTGELLAPFGDMTF
ECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHCCCCCCCH
DEAYEVFKEVVVAAEKAGADIVIIETMSDMLEAKAAILAAKENTNMKVICTMTFQEDGRT
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHEEEECCCCEEEEEEEEECCCCCE
LMGSDPVTVVVSLQGLGLDAIGVNCSTGPDKMVKVVEKMAQVARIPIIAQPNAGMPVIRD
EECCCCEEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCEEEC
GKTVYDLKPEEFASFFPQLVEKGACIVGGCCGTTPYYIKLVKKAVENLKPKKLENKFTAL
CCEEEECCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHC
SSNTRTVFIGKEYPLRVIGERINPTGKKKLSGALLSGDVNLAVEEALKQQKCGAEILDVN
CCCCEEEEEECCCCHHHHHCCCCCCCHHHHCCEEECCCCHHHHHHHHHHHHCCCEEEEEE
VGVPGIEEEEMLPKVAFEVEKAVDLPLQLDSTNVKAIEKAIRILRGRPIINSVNAKEESL
CCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCCHHHH
KEVLPIVKKYGACVVGLTVGDKGLPKDRHERVENARKILKKAEEYGIPKEDVIIDPVVLT
HHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCEEEE
VSSEQGAAIETLEAMKIISEELGVNTVVGLSNISFGLPERKLINSTFLAMAASHGLTAAI
EECCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEE
VNPCDESLMDTLRASMVLLNKDKGSENYLKVYGQRVKPKEEVIKRHEEDFSKKLYDQILE
ECCCHHHHHHHHHHHEEEEECCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHC
GKKSGVEEIVMALLEDNPPLSLVDEIIIPALKEVGDRYEKGIYFLPQLLSSAEVVQSAFK
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LIKEKLPKGTASKGKIILATVEGDVHDIGKNIVKVLLENYGYEVIDLGKDVKGEVIVEEV
HHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHH
KKTGAPLVGLSALMTTTLFNMEKIIKMLKENTEAKVMVGGAVLTEEYAFKIGADYYGKTA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHEECCCCCCCCH
QDAVKIADKFFLKNALVCKTCSF
HHHHHHHHHHHHHCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]