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The map label for this gene is TrmA [C]

Identifier: 20808214

GI number: 20808214

Start: 1742497

End: 1743849

Strand: Reverse

Name: TrmA [C]

Synonym: TTE1797

Alternate gene names: 20808214

Gene position: 1743849-1742497 (Counterclockwise)

Preceding gene: 20808219

Following gene: 20808213

Centisome position: 64.84

GC content: 38.14

Gene sequence:

>1353_bases
ATGGCGAAAGGTGAAATTGTCACAGTAAAAATTGAAGAGATGGATTTTAAAGGCTATGGGGTAGGGTATTGCGAAGGAAA
ACCTCTAAAAGTAAGGGGAGGAATTTTAGGTCAAAGAGTTGCTGTTAGGGTCAAAAAAGGCAAGAAAGGAAGAGCAGAAG
GAGAAATTGTTGAGGTCATTGAAAGGTCCCCTTTTGAAGTGGAAAGCCCTTGCCCTCATTTTGGGGAGTGTGGGGGGTGT
ACTTATCAAAATCTCTCTTACGAGAATCAGTTGAAAATAAAAAAAGATGTGGTTTTAAAATTGCTTGAAAAAGAAGGGAT
AAAGGATTTTGAATTTTTGGGAATAGAAAGAAGTCCAAAAGTATACGGCTACAGAAACAAAATGGAGTATACTTTTGGCA
CTGACAGGGAAGGGAAGAAAGCTTTAGGCCTTCACAGAAAAGGAAAATTTTATGATGTAGTTATGACTGATAAGTGCAAT
ATAGTCGACGAGGATTTTACTAAAGCCCTTTCACTCACTTTCAATTACGCTTTAGAAAAGAACCTGCCTTTTTATGATAA
AAAGACCCATGAAGGATATCTCAGGTATCTTGTGGTGAGAAAGGCATCAAAAAACGGGGAACTTTTGATAAATATAGTTA
CCACGACTCAGCTTAAACACAAATTTGATGACCTCGTAGAACTTTATAGAAGTGCAAATTTTTCTAGTACCTTGGTAGGG
GTTCTTCACACTTACAATGATTCCTGGTCTGATGCTGTTATATGCGAAAAACTGGAAGTCCTTTATGGAAGGGACTATTT
GATAGAGGAATTGCTGGGGCTTAAGTTTAAAATAAGCGCTTTTTCGTTTTTCCAGACGAATTCCTATGGTGCTGAGAGGA
TATATTCTACAGTTAGAGAATTTGCGGGAGATGTGTCTAATAAGACTATATTTGACCTTTACTGTGGCACAGGAACTATA
GGTATAGTAATGGCTCCTTTGGCGAGAAAAGTTATAGGAATAGAGCTGGTTGAAGAAGCAGTTTTTTCGGCAAAGGAGAA
TGCTGCCTTAAACGGCCTTGAAAATGCAGTATTTATAGCTGGGGACGTGTCAAAAAAATTGAGAGAAATAAAAGAGAAGC
CAGATATAGTGGTAGTAGATCCTCCGAGGTCAGGGGTTAATCCAAAAGCGCTAGAAGACATTGTAAATTTTGAACCTGAG
AAGATAATTTATGTTTCCTGCAATCCAGAGTCTTTTGCAAGAGATTTAAGGTTGTTTGTCGACAAAGGATATGCTGTTAA
AAAGGTAAAGGCGATAGACATGTTTCCTCACACCTATCATGTGGAGTTAGTAGGGCTTCTTGAAAAGAGTTAA

Upstream 100 bases:

>100_bases
TACAATTTTATTTAGTTTATGTAAAGGTTTTTTGAAAATTCCCATTAGAAATATCGGAGGAATTGGGGTATAATGTTAGT
GTAAGAAGGAGGATAGGAGA

Downstream 100 bases:

>100_bases
GGAGAGAAGACAGTCATGTTAGTTAGTTTGGAAATGGAGGTAGTTAGATGTCAATAAGAGAGATTGATATAAAGGAAATT
GAAGGGATAAAAGTAGGCAA

Product: SAM-dependent methyltransferase related to tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MAKGEIVTVKIEEMDFKGYGVGYCEGKPLKVRGGILGQRVAVRVKKGKKGRAEGEIVEVIERSPFEVESPCPHFGECGGC
TYQNLSYENQLKIKKDVVLKLLEKEGIKDFEFLGIERSPKVYGYRNKMEYTFGTDREGKKALGLHRKGKFYDVVMTDKCN
IVDEDFTKALSLTFNYALEKNLPFYDKKTHEGYLRYLVVRKASKNGELLINIVTTTQLKHKFDDLVELYRSANFSSTLVG
VLHTYNDSWSDAVICEKLEVLYGRDYLIEELLGLKFKISAFSFFQTNSYGAERIYSTVREFAGDVSNKTIFDLYCGTGTI
GIVMAPLARKVIGIELVEEAVFSAKENAALNGLENAVFIAGDVSKKLREIKEKPDIVVVDPPRSGVNPKALEDIVNFEPE
KIIYVSCNPESFARDLRLFVDKGYAVKKVKAIDMFPHTYHVELVGLLEKS

Sequences:

>Translated_450_residues
MAKGEIVTVKIEEMDFKGYGVGYCEGKPLKVRGGILGQRVAVRVKKGKKGRAEGEIVEVIERSPFEVESPCPHFGECGGC
TYQNLSYENQLKIKKDVVLKLLEKEGIKDFEFLGIERSPKVYGYRNKMEYTFGTDREGKKALGLHRKGKFYDVVMTDKCN
IVDEDFTKALSLTFNYALEKNLPFYDKKTHEGYLRYLVVRKASKNGELLINIVTTTQLKHKFDDLVELYRSANFSSTLVG
VLHTYNDSWSDAVICEKLEVLYGRDYLIEELLGLKFKISAFSFFQTNSYGAERIYSTVREFAGDVSNKTIFDLYCGTGTI
GIVMAPLARKVIGIELVEEAVFSAKENAALNGLENAVFIAGDVSKKLREIKEKPDIVVVDPPRSGVNPKALEDIVNFEPE
KIIYVSCNPESFARDLRLFVDKGYAVKKVKAIDMFPHTYHVELVGLLEKS
>Mature_449_residues
AKGEIVTVKIEEMDFKGYGVGYCEGKPLKVRGGILGQRVAVRVKKGKKGRAEGEIVEVIERSPFEVESPCPHFGECGGCT
YQNLSYENQLKIKKDVVLKLLEKEGIKDFEFLGIERSPKVYGYRNKMEYTFGTDREGKKALGLHRKGKFYDVVMTDKCNI
VDEDFTKALSLTFNYALEKNLPFYDKKTHEGYLRYLVVRKASKNGELLINIVTTTQLKHKFDDLVELYRSANFSSTLVGV
LHTYNDSWSDAVICEKLEVLYGRDYLIEELLGLKFKISAFSFFQTNSYGAERIYSTVREFAGDVSNKTIFDLYCGTGTIG
IVMAPLARKVIGIELVEEAVFSAKENAALNGLENAVFIAGDVSKKLREIKEKPDIVVVDPPRSGVNPKALEDIVNFEPEK
IIYVSCNPESFARDLRLFVDKGYAVKKVKAIDMFPHTYHVELVGLLEKS

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI269784680, Length=418, Percent_Identity=29.6650717703349, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI269784676, Length=418, Percent_Identity=29.6650717703349, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI20149688, Length=418, Percent_Identity=29.6650717703349, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI34222389, Length=368, Percent_Identity=29.0760869565217, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI51173878, Length=368, Percent_Identity=29.0760869565217, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI269784678, Length=365, Percent_Identity=30.1369863013699, Blast_Score=139, Evalue=5e-33,
Organism=Escherichia coli, GI1789148, Length=463, Percent_Identity=27.645788336933, Blast_Score=161, Evalue=6e-41,
Organism=Escherichia coli, GI1787083, Length=383, Percent_Identity=27.4151436031332, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1790403, Length=166, Percent_Identity=28.9156626506024, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71997438, Length=165, Percent_Identity=31.5151515151515, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6322909, Length=450, Percent_Identity=25.3333333333333, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24666579, Length=472, Percent_Identity=29.2372881355932, Blast_Score=163, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1797_THETN (Q8R933)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623385.1
- ProteinModelPortal:   Q8R933
- SMR:   Q8R933
- GeneID:   996976
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1797
- NMPDR:   fig|273068.3.peg.1327
- HOGENOM:   HBG690163
- OMA:   LNGLNNC
- ProtClustDB:   CLSK2754785
- BioCyc:   TTEN273068:TTE1797-MONOMER
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: NA

Molecular weight: Translated: 50782; Mature: 50651

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS50926 TRAM; PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 407-407 BINDING 285-285 BINDING 314-314 BINDING 335-335 BINDING 380-380

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGEIVTVKIEEMDFKGYGVGYCEGKPLKVRGGILGQRVAVRVKKGKKGRAEGEIVEVI
CCCCCEEEEEEEEECCCCCCEEEECCCCEEEECCCCCCEEEEEEECCCCCCCCCHHHHHH
ERSPFEVESPCPHFGECGGCTYQNLSYENQLKIKKDVVLKLLEKEGIKDFEFLGIERSPK
HCCCCCCCCCCCCCCCCCCCEECCCCCCCCEEHHHHHHHHHHHHCCCCCEEEECCCCCCC
VYGYRNKMEYTFGTDREGKKALGLHRKGKFYDVVMTDKCNIVDEDFTKALSLTFNYALEK
EEEECCCEEEECCCCCCCCHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHEEEEEEEC
NLPFYDKKTHEGYLRYLVVRKASKNGELLINIVTTTQLKHKFDDLVELYRSANFSSTLVG
CCCCCCCCCCCCCEEEEEEEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCHHHHH
VLHTYNDSWSDAVICEKLEVLYGRDYLIEELLGLKFKISAFSFFQTNSYGAERIYSTVRE
HHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCEEEEEEEEEEECCCCCHHHHHHHHHH
FAGDVSNKTIFDLYCGTGTIGIVMAPLARKVIGIELVEEAVFSAKENAALNGLENAVFIA
HHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEEE
GDVSKKLREIKEKPDIVVVDPPRSGVNPKALEDIVNFEPEKIIYVSCNPESFARDLRLFV
CCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHH
DKGYAVKKVKAIDMFPHTYHVELVGLLEKS
CCCCEEEEEEEEECCCCEEEEEEEEEECCC
>Mature Secondary Structure 
AKGEIVTVKIEEMDFKGYGVGYCEGKPLKVRGGILGQRVAVRVKKGKKGRAEGEIVEVI
CCCCEEEEEEEEECCCCCCEEEECCCCEEEECCCCCCEEEEEEECCCCCCCCCHHHHHH
ERSPFEVESPCPHFGECGGCTYQNLSYENQLKIKKDVVLKLLEKEGIKDFEFLGIERSPK
HCCCCCCCCCCCCCCCCCCCEECCCCCCCCEEHHHHHHHHHHHHCCCCCEEEECCCCCCC
VYGYRNKMEYTFGTDREGKKALGLHRKGKFYDVVMTDKCNIVDEDFTKALSLTFNYALEK
EEEECCCEEEECCCCCCCCHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHEEEEEEEC
NLPFYDKKTHEGYLRYLVVRKASKNGELLINIVTTTQLKHKFDDLVELYRSANFSSTLVG
CCCCCCCCCCCCCEEEEEEEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCHHHHH
VLHTYNDSWSDAVICEKLEVLYGRDYLIEELLGLKFKISAFSFFQTNSYGAERIYSTVRE
HHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCEEEEEEEEEEECCCCCHHHHHHHHHH
FAGDVSNKTIFDLYCGTGTIGIVMAPLARKVIGIELVEEAVFSAKENAALNGLENAVFIA
HHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEEE
GDVSKKLREIKEKPDIVVVDPPRSGVNPKALEDIVNFEPEKIIYVSCNPESFARDLRLFV
CCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHH
DKGYAVKKVKAIDMFPHTYHVELVGLLEKS
CCCCEEEEEEEEECCCCEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336