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The map label for this gene is PckA

Identifier: 20808202

GI number: 20808202

Start: 1729676

End: 1731241

Strand: Direct

Name: PckA

Synonym: TTE1783

Alternate gene names: 20808202

Gene position: 1729676-1731241 (Clockwise)

Preceding gene: 20808200

Following gene: 20808215

Centisome position: 64.31

GC content: 38.7

Gene sequence:

>1566_bases
ATGCTTAACCTTGAAAGATTGGGTTTAATCAATTTTAAAAACGTTTACCGAAATTTGCCCGTAGCAAAACTGATAGAAGA
AGCTGTAAAAAGAGAGGAAGGAGTACTTGTAAATAACGGCGCCTTTAATGTGTACACTGGAAAATACACAGGTCGATCTC
CAAATGACAAGTTCATTGTAGACGAACCAGAAGTTCATGAGGATATATGGTGGGAAAATAACAAGCCTATTTCCGTAGAA
AAGTTTGAAAAGCTGTACAATAGGCTTATTGCCTATTTACAAAACAGAGACCTTTTTATTTTCGACGGATTTGTAGGTGC
AGACCCTCAATATAGAGTCTCCATAAGAGTGATAACAGAATACGCTTATCAAAGTTTAATGGCAAGACAGCTTTTCATAC
GCCCCACTGAAAAAGAATTACAAAATTTTGTCCCGGAATATACTTTAATCGCAGCACCTCGCTTTAAAGCAATCCCCGAA
ATTGACGGTGTTAACTCTGAGGCTTTTATCATCTTAAGTTTTACAAAAAAGCTCATAATTATTGGAGGCACCCAGTACGG
TGGAGAAATCAAAAAATCCATATTCACCTTAATGAACTACCTTATGCCTAAAAAAGGCGTGCTTTCCATGCATTGTTCTG
CTAATATAGGAAAAGACGGTGACACAGCCCTTTTCTTCGGCCTCTCCGGTACAGGAAAGACCACTCTCTCTGCAGACCCT
GAAAGATTTTTAATTGGCGATGATGAACACGGTTGGTCTGAAAGAGGGATTTTTAATTTTGAAGGTGGCTGTTATGCTAA
GTGCATCAATCTTTCAAGAGAAAAAGAGCCTCAAATATGGGATTCAATAAGGTTTGGCGCAATTCTTGAAAACGTTGTAT
ACGATGAAGCCACAAGGGAACTTGACTACACAAGTGACAAAATCACTGAAAACACGCGAGCAGCATATCCAATTGACTTT
ATACCTGGGGCAGTTCAAAGCGGGATAGGAGGACATCCTAAGACTATAATATTTTTGACTGCAGATGCGTTTGGAGTACT
GCCTCCTATAGCAAAACTCACAAAAGAGCAGGCAATGTACTACTTTCTGTCCGGATATACCAGTAAACTCGCTGGAACAG
AAAGGGGAATTACAGAGCCACAAGCCACTTTTTCCACTTGCTTTGGTGCTCCCTTCTTACCTTTACCTCCAATGGTATAT
GCTAAAATGCTTGGAGAAAAAATTGAAAAATACGACACAAAAGTCTTTTTGGTGAATACTGGATGGTCAGGAGGCCCTTA
TGGAATTGGAAAGCGAATAAACTTAGAATACACCAGAAAAATGGTATCTGCTGCCTTAAAAGGAGAATTAGATAAAGTAG
AATTTACCAAAGACCCTATATTTAATCTTAACATCCCTGCTTCTTGTCCCGGAGTACCTTCTGAAATCTTAAACCCCAGA
AATACATGGAAAGATAAAGAAGAGTATGACAAAACGGCAAAAAGACTAGCCCAAAGGTTTATAGAAAATTTTCAAAAATA
CAAAGAGGTAAGTGAAGAAATAAAAAATGCTGGCCCGAAAGGGTAA

Upstream 100 bases:

>100_bases
ATTAGAAATTACAATTTTATACTTAATAAGATATTTTTTGCAATTCAATCACCCTAAAATTGCAAAAATTACTTCTAAAT
CTAAAAAGGAGGCCTCATAA

Downstream 100 bases:

>100_bases
AAACTAGTTCTCTCTGCGCATCGCAGAGAGAACTTTTTATTTGCCTTTTACATATTATGAAATTGAACCTGCATAAAGGA
GGTCGTAAATATGCCTGGCA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MLNLERLGLINFKNVYRNLPVAKLIEEAVKREEGVLVNNGAFNVYTGKYTGRSPNDKFIVDEPEVHEDIWWENNKPISVE
KFEKLYNRLIAYLQNRDLFIFDGFVGADPQYRVSIRVITEYAYQSLMARQLFIRPTEKELQNFVPEYTLIAAPRFKAIPE
IDGVNSEAFIILSFTKKLIIIGGTQYGGEIKKSIFTLMNYLMPKKGVLSMHCSANIGKDGDTALFFGLSGTGKTTLSADP
ERFLIGDDEHGWSERGIFNFEGGCYAKCINLSREKEPQIWDSIRFGAILENVVYDEATRELDYTSDKITENTRAAYPIDF
IPGAVQSGIGGHPKTIIFLTADAFGVLPPIAKLTKEQAMYYFLSGYTSKLAGTERGITEPQATFSTCFGAPFLPLPPMVY
AKMLGEKIEKYDTKVFLVNTGWSGGPYGIGKRINLEYTRKMVSAALKGELDKVEFTKDPIFNLNIPASCPGVPSEILNPR
NTWKDKEEYDKTAKRLAQRFIENFQKYKEVSEEIKNAGPKG

Sequences:

>Translated_521_residues
MLNLERLGLINFKNVYRNLPVAKLIEEAVKREEGVLVNNGAFNVYTGKYTGRSPNDKFIVDEPEVHEDIWWENNKPISVE
KFEKLYNRLIAYLQNRDLFIFDGFVGADPQYRVSIRVITEYAYQSLMARQLFIRPTEKELQNFVPEYTLIAAPRFKAIPE
IDGVNSEAFIILSFTKKLIIIGGTQYGGEIKKSIFTLMNYLMPKKGVLSMHCSANIGKDGDTALFFGLSGTGKTTLSADP
ERFLIGDDEHGWSERGIFNFEGGCYAKCINLSREKEPQIWDSIRFGAILENVVYDEATRELDYTSDKITENTRAAYPIDF
IPGAVQSGIGGHPKTIIFLTADAFGVLPPIAKLTKEQAMYYFLSGYTSKLAGTERGITEPQATFSTCFGAPFLPLPPMVY
AKMLGEKIEKYDTKVFLVNTGWSGGPYGIGKRINLEYTRKMVSAALKGELDKVEFTKDPIFNLNIPASCPGVPSEILNPR
NTWKDKEEYDKTAKRLAQRFIENFQKYKEVSEEIKNAGPKG
>Mature_521_residues
MLNLERLGLINFKNVYRNLPVAKLIEEAVKREEGVLVNNGAFNVYTGKYTGRSPNDKFIVDEPEVHEDIWWENNKPISVE
KFEKLYNRLIAYLQNRDLFIFDGFVGADPQYRVSIRVITEYAYQSLMARQLFIRPTEKELQNFVPEYTLIAAPRFKAIPE
IDGVNSEAFIILSFTKKLIIIGGTQYGGEIKKSIFTLMNYLMPKKGVLSMHCSANIGKDGDTALFFGLSGTGKTTLSADP
ERFLIGDDEHGWSERGIFNFEGGCYAKCINLSREKEPQIWDSIRFGAILENVVYDEATRELDYTSDKITENTRAAYPIDF
IPGAVQSGIGGHPKTIIFLTADAFGVLPPIAKLTKEQAMYYFLSGYTSKLAGTERGITEPQATFSTCFGAPFLPLPPMVY
AKMLGEKIEKYDTKVFLVNTGWSGGPYGIGKRINLEYTRKMVSAALKGELDKVEFTKDPIFNLNIPASCPGVPSEILNPR
NTWKDKEEYDKTAKRLAQRFIENFQKYKEVSEEIKNAGPKG

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=537, Percent_Identity=47.2998137802607, Blast_Score=495, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6322950, Length=515, Percent_Identity=49.9029126213592, Blast_Score=513, Evalue=1e-146,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_THETN (Q8R943)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623373.1
- ProteinModelPortal:   Q8R943
- SMR:   Q8R943
- GeneID:   996865
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1783
- NMPDR:   fig|273068.3.peg.1315
- HOGENOM:   HBG293387
- OMA:   MFIRPTE
- ProtClustDB:   PRK09344
- BioCyc:   TTEN273068:TTE1783-MONOMER
- BRENDA:   4.1.1.49
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 58772; Mature: 58772

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLERLGLINFKNVYRNLPVAKLIEEAVKREEGVLVNNGAFNVYTGKYTGRSPNDKFIV
CCCHHHCCCCCHHHHHHCCCHHHHHHHHHHHHCCEEEECCEEEEEECCCCCCCCCCCEEE
DEPEVHEDIWWENNKPISVEKFEKLYNRLIAYLQNRDLFIFDGFVGADPQYRVSIRVITE
CCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEHHHH
YAYQSLMARQLFIRPTEKELQNFVPEYTLIAAPRFKAIPEIDGVNSEAFIILSFTKKLII
HHHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEE
IGGTQYGGEIKKSIFTLMNYLMPKKGVLSMHCSANIGKDGDTALFFGLSGTGKTTLSADP
EECCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCC
ERFLIGDDEHGWSERGIFNFEGGCYAKCINLSREKEPQIWDSIRFGAILENVVYDEATRE
CEEEECCCCCCCCCCCEEECCCCCEEEHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LDYTSDKITENTRAAYPIDFIPGAVQSGIGGHPKTIIFLTADAFGVLPPIAKLTKEQAMY
CCCCCHHHCCCCCCCCCHHHCCHHHHCCCCCCCCEEEEEECCCHHCCCHHHHHHHHHHHH
YFLSGYTSKLAGTERGITEPQATFSTCFGAPFLPLPPMVYAKMLGEKIEKYDTKVFLVNT
HHHHCHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEC
GWSGGPYGIGKRINLEYTRKMVSAALKGELDKVEFTKDPIFNLNIPASCPGVPSEILNPR
CCCCCCCCCCCEECHHHHHHHHHHHHHCCCCCEEECCCCEEEECCCCCCCCCCHHHCCCC
NTWKDKEEYDKTAKRLAQRFIENFQKYKEVSEEIKNAGPKG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLNLERLGLINFKNVYRNLPVAKLIEEAVKREEGVLVNNGAFNVYTGKYTGRSPNDKFIV
CCCHHHCCCCCHHHHHHCCCHHHHHHHHHHHHCCEEEECCEEEEEECCCCCCCCCCCEEE
DEPEVHEDIWWENNKPISVEKFEKLYNRLIAYLQNRDLFIFDGFVGADPQYRVSIRVITE
CCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEHHHH
YAYQSLMARQLFIRPTEKELQNFVPEYTLIAAPRFKAIPEIDGVNSEAFIILSFTKKLII
HHHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEE
IGGTQYGGEIKKSIFTLMNYLMPKKGVLSMHCSANIGKDGDTALFFGLSGTGKTTLSADP
EECCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCC
ERFLIGDDEHGWSERGIFNFEGGCYAKCINLSREKEPQIWDSIRFGAILENVVYDEATRE
CEEEECCCCCCCCCCCEEECCCCCEEEHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LDYTSDKITENTRAAYPIDFIPGAVQSGIGGHPKTIIFLTADAFGVLPPIAKLTKEQAMY
CCCCCHHHCCCCCCCCCHHHCCHHHHCCCCCCCCEEEEEECCCHHCCCHHHHHHHHHHHH
YFLSGYTSKLAGTERGITEPQATFSTCFGAPFLPLPPMVYAKMLGEKIEKYDTKVFLVNT
HHHHCHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEC
GWSGGPYGIGKRINLEYTRKMVSAALKGELDKVEFTKDPIFNLNIPASCPGVPSEILNPR
CCCCCCCCCCCEECHHHHHHHHHHHHHCCCCCEEECCCCEEEECCCCCCCCCCHHHCCCC
NTWKDKEEYDKTAKRLAQRFIENFQKYKEVSEEIKNAGPKG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336