Click here to switch to the map view.

The map label for this gene is Cof2 [H]

Identifier: 20808185

GI number: 20808185

Start: 1713299

End: 1714120

Strand: Reverse

Name: Cof2 [H]

Synonym: TTE1766

Alternate gene names: 20808185

Gene position: 1714120-1713299 (Counterclockwise)

Preceding gene: 20808187

Following gene: 20808184

Centisome position: 63.74

GC content: 37.47

Gene sequence:

>822_bases
GTGCAGTATAAGCTAATTGCTATTGATATGGATGATACGCTTTTAAGGCACGATAAGACAATTTCTGAGGAGAACATTAA
GGCGCTCCACAGGGCAAGAGAAAAGGGAGTGTATGTAGTGATTGCGACGGGAAGAGTGTTTGCCTCTGCTTACGCTTATG
CTGATATGATCGGTTTTAGAACTTATATTATTGCAAGCAATGGAGCTTTGATAAGGGACCCAGATAACAACACTATCTAT
GAAAGCATTTTAGATTACAACAACATGATAGAGGTGATAAGAACCTGTCAAAAATATGGAACCTATTTTCAGTTGATAAG
CGATACCACAGTGTACACTCCAGAGATTACAAATAAATTCCAGAGATATGCTGAATGGAATGCCCTTTTGAAAACAGAAA
TAAAAGTAGAAGTCGAAGAAATAGAAAATCCATTGGAAGAAGCGGAAAAACTTAAAGACAGGGTTTTGAAAGTGATAGTT
TTCAATGAGGATCCAGACCTATTAAAAAGAATTAGAAGTGAGCTTTCTGAGAGGTTAGATGTGCAGATTACCAGCTCTTA
CGTGGACAACATTGAAATCATGAACAAGGGGGTCAGCAAAGGAAGGGCTTTAGAAATTCTTGGAAGATATCTTGGAGTCT
CTAGAGAAGAGATGATAGCTATTGGAGACAGTGAGAATGACATAGAAATGATTAAATTTGCGGGATTAGGGGTGGCTATG
GAGAATGCTATTGATGAAGTGAAAAAAGTAGCAGACTTTATCACAAAGTCCAACATGGAAGATGGGGTGAAACACGTTAT
TGAAAAATTTATATTAACTTGA

Upstream 100 bases:

>100_bases
TTTAATAATGTCAATCGTTTTTATTAGAAAGGCGTATCGACTGAACTTTCGAAATAAGGTATAATATAACTGAACAGAAT
AGCAAAAGGAGTTGAAAAAA

Downstream 100 bases:

>100_bases
GAGAGGGGATTTTGTTATGAAAAAGACTGTTCATTTGAGGATAACTGGACATGTCCAGGGAGTGGGGCTCAGGTATTCTG
TGTATCAGAAGGCTACGTCT

Product: HAD superfamily hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MQYKLIAIDMDDTLLRHDKTISEENIKALHRAREKGVYVVIATGRVFASAYAYADMIGFRTYIIASNGALIRDPDNNTIY
ESILDYNNMIEVIRTCQKYGTYFQLISDTTVYTPEITNKFQRYAEWNALLKTEIKVEVEEIENPLEEAEKLKDRVLKVIV
FNEDPDLLKRIRSELSERLDVQITSSYVDNIEIMNKGVSKGRALEILGRYLGVSREEMIAIGDSENDIEMIKFAGLGVAM
ENAIDEVKKVADFITKSNMEDGVKHVIEKFILT

Sequences:

>Translated_273_residues
MQYKLIAIDMDDTLLRHDKTISEENIKALHRAREKGVYVVIATGRVFASAYAYADMIGFRTYIIASNGALIRDPDNNTIY
ESILDYNNMIEVIRTCQKYGTYFQLISDTTVYTPEITNKFQRYAEWNALLKTEIKVEVEEIENPLEEAEKLKDRVLKVIV
FNEDPDLLKRIRSELSERLDVQITSSYVDNIEIMNKGVSKGRALEILGRYLGVSREEMIAIGDSENDIEMIKFAGLGVAM
ENAIDEVKKVADFITKSNMEDGVKHVIEKFILT
>Mature_273_residues
MQYKLIAIDMDDTLLRHDKTISEENIKALHRAREKGVYVVIATGRVFASAYAYADMIGFRTYIIASNGALIRDPDNNTIY
ESILDYNNMIEVIRTCQKYGTYFQLISDTTVYTPEITNKFQRYAEWNALLKTEIKVEVEEIENPLEEAEKLKDRVLKVIV
FNEDPDLLKRIRSELSERLDVQITSSYVDNIEIMNKGVSKGRALEILGRYLGVSREEMIAIGDSENDIEMIKFAGLGVAM
ENAIDEVKKVADFITKSNMEDGVKHVIEKFILT

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates erythrose 4-phosphate and mannose 1-phosphate [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI2367265, Length=281, Percent_Identity=38.7900355871886, Blast_Score=165, Evalue=3e-42,
Organism=Escherichia coli, GI1786982, Length=276, Percent_Identity=28.9855072463768, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI48994981, Length=281, Percent_Identity=29.1814946619217, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87081741, Length=251, Percent_Identity=27.0916334661355, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87081790, Length=286, Percent_Identity=26.5734265734266, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787043, Length=271, Percent_Identity=22.8782287822878, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 31255; Mature: 31255

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYKLIAIDMDDTLLRHDKTISEENIKALHRAREKGVYVVIATGRVFASAYAYADMIGFR
CCEEEEEEECCHHHHHCCCCCCHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHE
TYIIASNGALIRDPDNNTIYESILDYNNMIEVIRTCQKYGTYFQLISDTTVYTPEITNKF
EEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHEECCCCEEECCHHHHHH
QRYAEWNALLKTEIKVEVEEIENPLEEAEKLKDRVLKVIVFNEDPDLLKRIRSELSERLD
HHHHHHHHHHHHHHEEEHHHHHCHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHC
VQITSSYVDNIEIMNKGVSKGRALEILGRYLGVSREEMIAIGDSENDIEMIKFAGLGVAM
EEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHEEEECCCCCCHHEEEECCCCHHH
ENAIDEVKKVADFITKSNMEDGVKHVIEKFILT
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQYKLIAIDMDDTLLRHDKTISEENIKALHRAREKGVYVVIATGRVFASAYAYADMIGFR
CCEEEEEEECCHHHHHCCCCCCHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHE
TYIIASNGALIRDPDNNTIYESILDYNNMIEVIRTCQKYGTYFQLISDTTVYTPEITNKF
EEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHEECCCCEEECCHHHHHH
QRYAEWNALLKTEIKVEVEEIENPLEEAEKLKDRVLKVIVFNEDPDLLKRIRSELSERLD
HHHHHHHHHHHHHHEEEHHHHHCHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHC
VQITSSYVDNIEIMNKGVSKGRALEILGRYLGVSREEMIAIGDSENDIEMIKFAGLGVAM
EEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHEEEECCCCCCHHEEEECCCCHHH
ENAIDEVKKVADFITKSNMEDGVKHVIEKFILT
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]