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The map label for this gene is SpeE2

Identifier: 20808167

GI number: 20808167

Start: 1695165

End: 1696058

Strand: Reverse

Name: SpeE2

Synonym: TTE1748

Alternate gene names: 20808167

Gene position: 1696058-1695165 (Counterclockwise)

Preceding gene: 20808168

Following gene: 20808166

Centisome position: 63.06

GC content: 38.37

Gene sequence:

>894_bases
GTGATATTAAAATTGGAGGTGGGAGTGGGGTTGAAAAATCTAAATGGAAAGTGGGGATTGGACTCATCTAAAGAGTTTTA
CTGGGAGCCAGATGTGGAAGGAGGCTATAGAGTTTACAAAGTAAAGTCTGTTATTATAGAACATCAGTCACTGTATCAGA
AGATAGACATTGTAGAACTTGAAACTTGGGGAAAGAGTTTATTTTTAGACGGCAGCCTTCAGTCAACAGAATCAGATGAA
TTTATATACCATGAACTTTTGGTGCATCCAGCTATGCGTGTTCACCCTTCTCCTAAGAGAGTTTTGATATGCGGGGTAGG
AGAGGGGAAGAGCGTAAGAGAAGTTTTAAAATACCCGACAGTAAAAGAGGTAATAGGTGTTGATATAGATAAAGAAGTAG
TTGCCCTCTGTCAGAAACACTTGGGTAAAACACCTATTGATGACAAGCGTGTGAGACTTGTATATCAAGATGTAGCAGAA
TTTATAAAAAATTATAGAGGCGAACCCTTTGATGTAGCTATAGTAGATGTAACAGATGATTTAGATGGGCCTGCTAGGAG
TGTTCACCAGTTAGACTTTTATAAAAGGCTTTATGAAATTTTAGGCGAAAAAGCTACTGTGGTAGTGCAGGGGACATCAG
CTTTTTCGAAAGTTAAAAATGTCGGCTTTTGCCACATCTATGAGATTCTCAAAGAAGTATTTCCTTTTGTAATTCCCTAT
GCCGATTACATCCCCTCTTTTAGCGATTTGTGGACTTTTTTTGTGGCTCTGAAAGGAATTAAAGACTTAACCCCAAGGCA
TGAAATCCCCCGTGACCTTAAGTATTACGATGAATATACAAATGAAAGGATTTTTACTCTAGCTAAGCCTTTGAGAGAAA
AACTAGGGGTATGA

Upstream 100 bases:

>100_bases
GTGACTTGGTTTTTTTCATGCTTAATTTATGGAATTATAAGGCAATAATTCCTGGAGCTGTTTGGCGAATACTTTTCAAT
ATGGTATAATATTTTAAGGC

Downstream 100 bases:

>100_bases
TAGTACCCCTTCAGACTGTTGACAAAATAAAGAAGTGGCATTTTGTGTGTAAATGCGACTTGTTGTCAAAGCGGTAACTA
AACTTGGCGAACTTGAGGAG

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase 2; PAPT 2; SPDSY 2

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MILKLEVGVGLKNLNGKWGLDSSKEFYWEPDVEGGYRVYKVKSVIIEHQSLYQKIDIVELETWGKSLFLDGSLQSTESDE
FIYHELLVHPAMRVHPSPKRVLICGVGEGKSVREVLKYPTVKEVIGVDIDKEVVALCQKHLGKTPIDDKRVRLVYQDVAE
FIKNYRGEPFDVAIVDVTDDLDGPARSVHQLDFYKRLYEILGEKATVVVQGTSAFSKVKNVGFCHIYEILKEVFPFVIPY
ADYIPSFSDLWTFFVALKGIKDLTPRHEIPRDLKYYDEYTNERIFTLAKPLREKLGV

Sequences:

>Translated_297_residues
MILKLEVGVGLKNLNGKWGLDSSKEFYWEPDVEGGYRVYKVKSVIIEHQSLYQKIDIVELETWGKSLFLDGSLQSTESDE
FIYHELLVHPAMRVHPSPKRVLICGVGEGKSVREVLKYPTVKEVIGVDIDKEVVALCQKHLGKTPIDDKRVRLVYQDVAE
FIKNYRGEPFDVAIVDVTDDLDGPARSVHQLDFYKRLYEILGEKATVVVQGTSAFSKVKNVGFCHIYEILKEVFPFVIPY
ADYIPSFSDLWTFFVALKGIKDLTPRHEIPRDLKYYDEYTNERIFTLAKPLREKLGV
>Mature_297_residues
MILKLEVGVGLKNLNGKWGLDSSKEFYWEPDVEGGYRVYKVKSVIIEHQSLYQKIDIVELETWGKSLFLDGSLQSTESDE
FIYHELLVHPAMRVHPSPKRVLICGVGEGKSVREVLKYPTVKEVIGVDIDKEVVALCQKHLGKTPIDDKRVRLVYQDVAE
FIKNYRGEPFDVAIVDVTDDLDGPARSVHQLDFYKRLYEILGEKATVVVQGTSAFSKVKNVGFCHIYEILKEVFPFVIPY
ADYIPSFSDLWTFFVALKGIKDLTPRHEIPRDLKYYDEYTNERIFTLAKPLREKLGV

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=268, Percent_Identity=29.4776119402985, Blast_Score=115, Evalue=5e-26,
Organism=Escherichia coli, GI1786312, Length=270, Percent_Identity=30.3703703703704, Blast_Score=109, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25154106, Length=268, Percent_Identity=32.4626865671642, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI212645616, Length=211, Percent_Identity=35.0710900473934, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6325326, Length=268, Percent_Identity=30.9701492537313, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323175, Length=262, Percent_Identity=30.9160305343511, Blast_Score=112, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24645443, Length=263, Percent_Identity=35.361216730038, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21355941, Length=211, Percent_Identity=32.2274881516588, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24663238, Length=259, Percent_Identity=27.7992277992278, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21358269, Length=228, Percent_Identity=28.0701754385965, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE2_THETN (Q8R977)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623338.1
- ProteinModelPortal:   Q8R977
- SMR:   Q8R977
- GeneID:   996767
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1748
- NMPDR:   fig|273068.3.peg.1280
- HOGENOM:   HBG635113
- OMA:   VEGGPCY
- ProtClustDB:   CLSK2519164
- BioCyc:   TTEN273068:TTE1748-MONOMER
- BRENDA:   2.5.1.16
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 34123; Mature: 34123

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: BINDING 53-53 BINDING 108-108 BINDING 128-128 BINDING 176-176 BINDING 179-179

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILKLEVGVGLKNLNGKWGLDSSKEFYWEPDVEGGYRVYKVKSVIIEHQSLYQKIDIVEL
CEEEEEECCCEECCCCCCCCCCCCCEEECCCCCCCEEEEEHHHHHHHHHHHHHHHCEEEE
ETWGKSLFLDGSLQSTESDEFIYHELLVHPAMRVHPSPKRVLICGVGEGKSVREVLKYPT
ECCCCEEEECCCCCCCCCCHHHHHHHHHCCHHHCCCCCCEEEEEECCCCHHHHHHHHCCC
VKEVIGVDIDKEVVALCQKHLGKTPIDDKRVRLVYQDVAEFIKNYRGEPFDVAIVDVTDD
HHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCC
LDGPARSVHQLDFYKRLYEILGEKATVVVQGTSAFSKVKNVGFCHIYEILKEVFPFVIPY
CCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHCH
ADYIPSFSDLWTFFVALKGIKDLTPRHEIPRDLKYYDEYTNERIFTLAKPLREKLGV
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MILKLEVGVGLKNLNGKWGLDSSKEFYWEPDVEGGYRVYKVKSVIIEHQSLYQKIDIVEL
CEEEEEECCCEECCCCCCCCCCCCCEEECCCCCCCEEEEEHHHHHHHHHHHHHHHCEEEE
ETWGKSLFLDGSLQSTESDEFIYHELLVHPAMRVHPSPKRVLICGVGEGKSVREVLKYPT
ECCCCEEEECCCCCCCCCCHHHHHHHHHCCHHHCCCCCCEEEEEECCCCHHHHHHHHCCC
VKEVIGVDIDKEVVALCQKHLGKTPIDDKRVRLVYQDVAEFIKNYRGEPFDVAIVDVTDD
HHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCC
LDGPARSVHQLDFYKRLYEILGEKATVVVQGTSAFSKVKNVGFCHIYEILKEVFPFVIPY
CCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHCH
ADYIPSFSDLWTFFVALKGIKDLTPRHEIPRDLKYYDEYTNERIFTLAKPLREKLGV
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336