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The map label for this gene is AceF3 [H]

Identifier: 20808094

GI number: 20808094

Start: 1616323

End: 1616982

Strand: Reverse

Name: AceF3 [H]

Synonym: TTE1668

Alternate gene names: 20808094

Gene position: 1616982-1616323 (Counterclockwise)

Preceding gene: 20808095

Following gene: 20808093

Centisome position: 60.12

GC content: 33.33

Gene sequence:

>660_bases
ATGGACACTATGAGAAGGACTATAAGCCAAAGAATGAAAAAAAGCTGGACAGAGATTCCTCATGTGACGGAAGACATCAA
AGTAGATGTGACAGAACTTGTTAATTTAAGAGAGAATTTAAACAAATCTGGAGAACATAAATTTACCTACACTGATTTGA
TTGCAAAAGCTTGTGTTATTGCATTAAAGAAAAATCCTGTTTTAAATTGGTCTATTGAGGGAGATGAAATAATTAAAAAT
CCTAACATCAACTTAGGAATTGCAGTGGCATTAGAGGATGGATTAATAGTTCCTGTGGTGAAGAATGCTGAAAATAAATC
TTTACTAGAGCTTTCAAAAGAGATTAAAGAACTAAGTGAAAAAGCAAGAGAGAATAAGCTAACTCCTGATGAAATAACAG
GTGGTACTTTTACTATCACTAACCTAGGAATGTATGAAATAGATAGCTTTACTCCTATTATAAATCCTCCAGAAAGTGCT
ATATTAGGAGTTAACAAAATATACAAAGAACCAGTGGTAATAGAAGACAATATTGTCATACGGCACACAATGAAACTTAG
TCTTTCTTTCGACCATCGACTGATTGATGGAGCTACTGCTGCTAAGTTTTTGCTCGACCTAAAGAAAATTTTGGAAAATC
CTGTATCCATGCTTATTTAA

Upstream 100 bases:

>100_bases
ATATATTTCTGAAAAGCAAATTGGTACTTTACCAGAGAAAACTGAAAAGGAAATAGTTTTAAGAGAAGAACAAATTCTCC
AAAAAGCAGAAAGAGTGCCA

Downstream 100 bases:

>100_bases
ATCTTTAAATGGCGGGCCATGTATTTTAGGCTCGCTTCTTTTATACTTTAAAAACTATGATATAAATTAGAGTTAACATA
TCGAAGGGAGGGTAACTCTT

Product: dihydrolipoamide acyltransferase

Products: NA

Alternate protein names: Acetoin dehydrogenase E2 component; Dihydrolipoamide acetyltransferase component of acetoin cleaving system [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MDTMRRTISQRMKKSWTEIPHVTEDIKVDVTELVNLRENLNKSGEHKFTYTDLIAKACVIALKKNPVLNWSIEGDEIIKN
PNINLGIAVALEDGLIVPVVKNAENKSLLELSKEIKELSEKARENKLTPDEITGGTFTITNLGMYEIDSFTPIINPPESA
ILGVNKIYKEPVVIEDNIVIRHTMKLSLSFDHRLIDGATAAKFLLDLKKILENPVSMLI

Sequences:

>Translated_219_residues
MDTMRRTISQRMKKSWTEIPHVTEDIKVDVTELVNLRENLNKSGEHKFTYTDLIAKACVIALKKNPVLNWSIEGDEIIKN
PNINLGIAVALEDGLIVPVVKNAENKSLLELSKEIKELSEKARENKLTPDEITGGTFTITNLGMYEIDSFTPIINPPESA
ILGVNKIYKEPVVIEDNIVIRHTMKLSLSFDHRLIDGATAAKFLLDLKKILENPVSMLI
>Mature_219_residues
MDTMRRTISQRMKKSWTEIPHVTEDIKVDVTELVNLRENLNKSGEHKFTYTDLIAKACVIALKKNPVLNWSIEGDEIIKN
PNINLGIAVALEDGLIVPVVKNAENKSLLELSKEIKELSEKARENKLTPDEITGGTFTITNLGMYEIDSFTPIINPPESA
ILGVNKIYKEPVVIEDNIVIRHTMKLSLSFDHRLIDGATAAKFLLDLKKILENPVSMLI

Specific function: The 2-Oxoglutarate Dehydrogenase Complex Catalyzes The Overall Conversion Of 2-Oxoglutarate To Succinyl-CoA And Co(2). It Contains Multiple Copies Of Three Enzymatic Components: 2- Oxoglutarate Dehydrogenase (E1), Dihydrolipoamide Succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=220, Percent_Identity=39.0909090909091, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI19923748, Length=225, Percent_Identity=36.8888888888889, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI203098816, Length=219, Percent_Identity=35.6164383561644, Blast_Score=150, Evalue=9e-37,
Organism=Homo sapiens, GI203098753, Length=219, Percent_Identity=35.6164383561644, Blast_Score=150, Evalue=9e-37,
Organism=Homo sapiens, GI260898739, Length=157, Percent_Identity=38.2165605095541, Blast_Score=119, Evalue=1e-27,
Organism=Homo sapiens, GI110671329, Length=223, Percent_Identity=35.4260089686099, Blast_Score=118, Evalue=4e-27,
Organism=Escherichia coli, GI1786946, Length=223, Percent_Identity=34.9775784753363, Blast_Score=153, Evalue=9e-39,
Organism=Escherichia coli, GI1786305, Length=211, Percent_Identity=39.8104265402844, Blast_Score=151, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17560088, Length=230, Percent_Identity=39.1304347826087, Blast_Score=152, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17538894, Length=217, Percent_Identity=37.3271889400922, Blast_Score=148, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI25146366, Length=220, Percent_Identity=35, Blast_Score=142, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI17537937, Length=222, Percent_Identity=31.981981981982, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320352, Length=224, Percent_Identity=41.5178571428571, Blast_Score=177, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6324258, Length=227, Percent_Identity=39.2070484581498, Blast_Score=160, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24645909, Length=218, Percent_Identity=36.697247706422, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI20129315, Length=222, Percent_Identity=36.4864864864865, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24582497, Length=222, Percent_Identity=36.4864864864865, Blast_Score=154, Evalue=3e-38,
Organism=Drosophila melanogaster, GI18859875, Length=224, Percent_Identity=32.1428571428571, Blast_Score=121, Evalue=4e-28,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 24617; Mature: 24617

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTMRRTISQRMKKSWTEIPHVTEDIKVDVTELVNLRENLNKSGEHKFTYTDLIAKACVI
CCHHHHHHHHHHHHHHHHCCCCCCHHEECHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
ALKKNPVLNWSIEGDEIIKNPNINLGIAVALEDGLIVPVVKNAENKSLLELSKEIKELSE
HCCCCCEEEEEECCCCEECCCCCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHH
KARENKLTPDEITGGTFTITNLGMYEIDSFTPIINPPESAILGVNKIYKEPVVIEDNIVI
HHHHCCCCCCCCCCCEEEEEECCEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEECCEEE
RHTMKLSLSFDHRLIDGATAAKFLLDLKKILENPVSMLI
EEEEEEEEECCCHHHCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDTMRRTISQRMKKSWTEIPHVTEDIKVDVTELVNLRENLNKSGEHKFTYTDLIAKACVI
CCHHHHHHHHHHHHHHHHCCCCCCHHEECHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
ALKKNPVLNWSIEGDEIIKNPNINLGIAVALEDGLIVPVVKNAENKSLLELSKEIKELSE
HCCCCCEEEEEECCCCEECCCCCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHH
KARENKLTPDEITGGTFTITNLGMYEIDSFTPIINPPESAILGVNKIYKEPVVIEDNIVI
HHHHCCCCCCCCCCCEEEEEECCEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEECCEEE
RHTMKLSLSFDHRLIDGATAAKFLLDLKKILENPVSMLI
EEEEEEEEECCCHHHCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]