Click here to switch to the map view.

The map label for this gene is RpoD3 [H]

Identifier: 20808064

GI number: 20808064

Start: 1587123

End: 1587848

Strand: Reverse

Name: RpoD3 [H]

Synonym: TTE1637

Alternate gene names: 20808064

Gene position: 1587848-1587123 (Counterclockwise)

Preceding gene: 20808065

Following gene: 20808063

Centisome position: 59.04

GC content: 35.67

Gene sequence:

>726_bases
GTGAAACTTAAAACAAAATTAAAAATAGAATTGCTATTGATATGGAGAAAACTTCTAGACTTTTTGGACCTTGCAGAAGA
GGTATACTACATAGGCGGAAGTGAAGCTTTGCCTCCTCCCCTTTCTGTGGAAGAGGAAAGTTATTTAATATCAAAAATGG
AAAAGGGAGATAACGAGGCAAAAAGCATTTTAATTGAGAGGAATTTGCGGCTGGTAGTTTATATAGCAAAAAAATTTGAG
AATACAGGAGTGGGAATTGAGGATTTAATATCAATCGGCACTATAGGACTCATAAAGGCTATAAATACTTTTAATCCCAG
GAAAAAGATAAAATTGGCCACATATGCTTCTCGCTGCATTGAAAATGAAATTTTGATGTATTTAAGGCGGAATAATAAGA
CAAGGCTGGAGGTTTCTTTTGATGAGCCTTTAAATGTTGACTGGGACGGAAATGAACTTTTGCTCTCAGATATTTTAGGC
ACTGACAACGAAACAGTGTATAAGTACATTGAGGACGAGGTTGAAAGAGAGCTTTTGACCTCTGCCTTAAAGAAGCTTCC
TGACAGAGAAAAGAAGATAATCAGGTTGAGATTTGGATTGGAGGGCGGTACGGAAAAAACTCAAAAAGAAGTAGCAGATA
TGCTAGGGATTTCTCAGTCTTACATTTCTAGGCTTGAAAAGAAAATTTTAAAAAGACTAAAGAAAGAAATGAACAGGTTA
GTATAA

Upstream 100 bases:

>100_bases
ACGGAAAAGAAATTAAAGACGTCATGCTTGGTATTTCTACAGACAAAATGGAGTTTGCATTGTTAAACCCCGAAATTATG
GGTTGATGGAGGTGTTTTTG

Downstream 100 bases:

>100_bases
ATTTTACAGTATATTTTCCAATCCTGCCGGCAATACTGTAAATGAGCTTAGCGGTTGGAGGCAGGATTGGCTATGAATAA
CAATAATAAAGTAGAAATTT

Product: sporulation sigma factor SigE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MKLKTKLKIELLLIWRKLLDFLDLAEEVYYIGGSEALPPPLSVEEESYLISKMEKGDNEAKSILIERNLRLVVYIAKKFE
NTGVGIEDLISIGTIGLIKAINTFNPRKKIKLATYASRCIENEILMYLRRNNKTRLEVSFDEPLNVDWDGNELLLSDILG
TDNETVYKYIEDEVERELLTSALKKLPDREKKIIRLRFGLEGGTEKTQKEVADMLGISQSYISRLEKKILKRLKKEMNRL
V

Sequences:

>Translated_241_residues
MKLKTKLKIELLLIWRKLLDFLDLAEEVYYIGGSEALPPPLSVEEESYLISKMEKGDNEAKSILIERNLRLVVYIAKKFE
NTGVGIEDLISIGTIGLIKAINTFNPRKKIKLATYASRCIENEILMYLRRNNKTRLEVSFDEPLNVDWDGNELLLSDILG
TDNETVYKYIEDEVERELLTSALKKLPDREKKIIRLRFGLEGGTEKTQKEVADMLGISQSYISRLEKKILKRLKKEMNRL
V
>Mature_241_residues
MKLKTKLKIELLLIWRKLLDFLDLAEEVYYIGGSEALPPPLSVEEESYLISKMEKGDNEAKSILIERNLRLVVYIAKKFE
NTGVGIEDLISIGTIGLIKAINTFNPRKKIKLATYASRCIENEILMYLRRNNKTRLEVSFDEPLNVDWDGNELLLSDILG
TDNETVYKYIEDEVERELLTSALKKLPDREKKIIRLRFGLEGGTEKTQKEVADMLGISQSYISRLEKKILKRLKKEMNRL
V

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor directs the transcription of crystal protein genes, a sporulation-regulated event [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=256, Percent_Identity=26.5625, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1789871, Length=103, Percent_Identity=41.747572815534, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1789448, Length=274, Percent_Identity=27.3722627737226, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014200
- InterPro:   IPR016263
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27868; Mature: 27868

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKTKLKIELLLIWRKLLDFLDLAEEVYYIGGSEALPPPLSVEEESYLISKMEKGDNEA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHCCCCHH
KSILIERNLRLVVYIAKKFENTGVGIEDLISIGTIGLIKAINTFNPRKKIKLATYASRCI
HHHHHHCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ENEILMYLRRNNKTRLEVSFDEPLNVDWDGNELLLSDILGTDNETVYKYIEDEVERELLT
HHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SALKKLPDREKKIIRLRFGLEGGTEKTQKEVADMLGISQSYISRLEKKILKRLKKEMNRL
HHHHHCCCHHHHEEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
V
C
>Mature Secondary Structure
MKLKTKLKIELLLIWRKLLDFLDLAEEVYYIGGSEALPPPLSVEEESYLISKMEKGDNEA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHCCCCHH
KSILIERNLRLVVYIAKKFENTGVGIEDLISIGTIGLIKAINTFNPRKKIKLATYASRCI
HHHHHHCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ENEILMYLRRNNKTRLEVSFDEPLNVDWDGNELLLSDILGTDNETVYKYIEDEVERELLT
HHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SALKKLPDREKKIIRLRFGLEGGTEKTQKEVADMLGISQSYISRLEKKILKRLKKEMNRL
HHHHHCCCHHHHEEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
V
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]