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The map label for this gene is RpoD2 [H]

Identifier: 20808063

GI number: 20808063

Start: 1586274

End: 1587068

Strand: Reverse

Name: RpoD2 [H]

Synonym: TTE1636

Alternate gene names: 20808063

Gene position: 1587068-1586274 (Counterclockwise)

Preceding gene: 20808064

Following gene: 20808062

Centisome position: 59.01

GC content: 37.86

Gene sequence:

>795_bases
TTGGAGGCAGGATTGGCTATGAATAACAATAATAAAGTAGAAATTTCAGGAGTAAACACCTCTAAATTGCCTGTTTTAAA
GCCCTCTAAACAGAGGGAACTTCTCATCAGGATGAAAAATGGCGATAAAAAGGCAAGGGAAGAGTTTATAAACAGCAACT
TAAGGCTTGTTTTAAGCGTTATACAGAGATTTAACAATAGGGGAGAGTCTGTGGATGACCTTTTTCAAGTGGGATGCATA
GGCCTTATTAAAGCCATTGACAATTTCGATTTAGATCAAAATGTGAAGTTTTCTACTTACGCTGTTCCGATGATAATAGG
AGAGATAAGGAGATATTTAAGAGATAATACTCCTATAAGGGTAAGTCGTTCTTTGAGAGACATAGCTTATAAAGCACTTC
AAGTGAGGGATAAACTCGTTTCTGAGAATTCCAAAGAACCGACAGTTGGAGAAATTGCTAAAGAGTTAGACCTTCCAAGC
GAAGAAGTAGTGATGGCACTGGACGCTATACAGGAGCCGGTTTCTTTGTTTGAGCCTATATATCACGATGGTGGAGATGC
CATATATGTGATGGATCAGGTAAGTGATGATAAAAATGTAGACGAGGTATGGCTTGAGAAAATTGCTTTAAAAGAAGCCT
TAGAGAAGTTAAATGAGAGAGAAAAGATGATACTTACAATGAGATTTTTTGAAGGAAAAACGCAGATGGAAGTCGCAAAA
GAGATAGGGATTTCACAAGCGCAGGTTTCTCGCCTGGAAAAAGCTGCATTAAATCATATGAGAAAGTACATCTAG

Upstream 100 bases:

>100_bases
GAAAATTTTAAAAAGACTAAAGAAAGAAATGAACAGGTTAGTATAAATTTTACAGTATATTTTCCAATCCTGCCGGCAAT
ACTGTAAATGAGCTTAGCGG

Downstream 100 bases:

>100_bases
CTTATCAGGAATGATAAGCTAGATTTTTTCATATAATGAGAGAAAAGAAAGTGAAAAGGGGAGAGGAAAATGATAAAAGC
GTCTGATTTGAGGGATAAAG

Product: sporulation sigma factor SigG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MEAGLAMNNNNKVEISGVNTSKLPVLKPSKQRELLIRMKNGDKKAREEFINSNLRLVLSVIQRFNNRGESVDDLFQVGCI
GLIKAIDNFDLDQNVKFSTYAVPMIIGEIRRYLRDNTPIRVSRSLRDIAYKALQVRDKLVSENSKEPTVGEIAKELDLPS
EEVVMALDAIQEPVSLFEPIYHDGGDAIYVMDQVSDDKNVDEVWLEKIALKEALEKLNEREKMILTMRFFEGKTQMEVAK
EIGISQAQVSRLEKAALNHMRKYI

Sequences:

>Translated_264_residues
MEAGLAMNNNNKVEISGVNTSKLPVLKPSKQRELLIRMKNGDKKAREEFINSNLRLVLSVIQRFNNRGESVDDLFQVGCI
GLIKAIDNFDLDQNVKFSTYAVPMIIGEIRRYLRDNTPIRVSRSLRDIAYKALQVRDKLVSENSKEPTVGEIAKELDLPS
EEVVMALDAIQEPVSLFEPIYHDGGDAIYVMDQVSDDKNVDEVWLEKIALKEALEKLNEREKMILTMRFFEGKTQMEVAK
EIGISQAQVSRLEKAALNHMRKYI
>Mature_264_residues
MEAGLAMNNNNKVEISGVNTSKLPVLKPSKQRELLIRMKNGDKKAREEFINSNLRLVLSVIQRFNNRGESVDDLFQVGCI
GLIKAIDNFDLDQNVKFSTYAVPMIIGEIRRYLRDNTPIRVSRSLRDIAYKALQVRDKLVSENSKEPTVGEIAKELDLPS
EEVVMALDAIQEPVSLFEPIYHDGGDAIYVMDQVSDDKNVDEVWLEKIALKEALEKLNEREKMILTMRFFEGKTQMEVAK
EIGISQAQVSRLEKAALNHMRKYI

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=239, Percent_Identity=31.7991631799163, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789098, Length=256, Percent_Identity=30.078125, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1789871, Length=263, Percent_Identity=27.3764258555133, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788231, Length=224, Percent_Identity=29.0178571428571, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014212
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 30100; Mature: 30100

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAGLAMNNNNKVEISGVNTSKLPVLKPSKQRELLIRMKNGDKKAREEFINSNLRLVLSV
CCCCCEECCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCHHHHHHH
IQRFNNRGESVDDLFQVGCIGLIKAIDNFDLDQNVKFSTYAVPMIIGEIRRYLRDNTPIR
HHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEE
VSRSLRDIAYKALQVRDKLVSENSKEPTVGEIAKELDLPSEEVVMALDAIQEPVSLFEPI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YHDGGDAIYVMDQVSDDKNVDEVWLEKIALKEALEKLNEREKMILTMRFFEGKTQMEVAK
HHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHCCCHHHHHHH
EIGISQAQVSRLEKAALNHMRKYI
HHCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEAGLAMNNNNKVEISGVNTSKLPVLKPSKQRELLIRMKNGDKKAREEFINSNLRLVLSV
CCCCCEECCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCHHHHHHH
IQRFNNRGESVDDLFQVGCIGLIKAIDNFDLDQNVKFSTYAVPMIIGEIRRYLRDNTPIR
HHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEE
VSRSLRDIAYKALQVRDKLVSENSKEPTVGEIAKELDLPSEEVVMALDAIQEPVSLFEPI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YHDGGDAIYVMDQVSDDKNVDEVWLEKIALKEALEKLNEREKMILTMRFFEGKTQMEVAK
HHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHCCCHHHHHHH
EIGISQAQVSRLEKAALNHMRKYI
HHCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961408; 7883192; 11466286 [H]