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The map label for this gene is Dur1.2 [H]

Identifier: 20808036

GI number: 20808036

Start: 1562296

End: 1563045

Strand: Reverse

Name: Dur1.2 [H]

Synonym: TTE1607

Alternate gene names: 20808036

Gene position: 1563045-1562296 (Counterclockwise)

Preceding gene: 20808037

Following gene: 20808035

Centisome position: 58.12

GC content: 37.47

Gene sequence:

>750_bases
ATGTACGAAAAACCCCGAATATTGCCTTTTGGCGACAAAGGAATTGTAGTTGAGTTTGGAAATGAAATAACCGTCGAATG
CAATGAAAAAGTTGTAAATCTTTATCGAATATTAAAGAAAGAGAGTAGACAAGGAATAATTTCTATGATTCCCACATATA
GGTCTCTCCTTGTAAAGTACAATCCTTTACAGATTTCCTACAATGAGCTGATAAAATATGTTTCAGAAAAAATGGATAAA
GAAGTAGAAATAAAAGAGGAATTTAAACCGCAGGTGTACGAAATACCAGTGGTATACGGGGGAGAATTTGGGCCTGACCT
TGAATTTGTAGCTAAACACAATAATTTATCTATTGAAGAAGTAATAGCAATTCACAGCGCTCCACTTTACAGAATTTATA
TGGTAGGTTTTACTATGGGATTTGCATATTTAGGTGGAATGTCCGAAAAAATTGCTACACCGCGCCTTGAGACTCCTCGC
ACAGAAATAAAGGCAGGGTCCGTGGGGATTGCTGGGAATCAGACTGGGATTTATCCTCTTTCGAGTCCGGGAGGATGGCG
AATAATAGGGAGAACTCCTGTGAGATTGTACGATCAGGAGAGAGAAAAGCCAATTTTATTTGAGGCAGGAAACTATATAA
AGTTCATTCCCGTCTCTGAAGAGGAATATTACAAGATAGAAAAAGAAATACAAGAGGGTAAATATCAACTGCGCATTTAC
GAATATCTAAATCATAATCTGAGTGGGTGA

Upstream 100 bases:

>100_bases
GGCTGTGGAGTTTGCAAAAAAGATAAGGGAAACTTTAGAAGAAAACGGAGTAGACATACTTCCAATGGGCGAATTTGTAT
AAAGTGAGGAGTTGATATAG

Downstream 100 bases:

>100_bases
TAAAATGGAATGTTTTAAAGTATTAGAGCCGGGATTATTTACTACTATTCAAGATTTAGGTAGGTTTGGCTATGAGAGCC
AGGGAGTTCCTACTTCTGGA

Product: allophanate hydrolase subunit 1

Products: CO2; NH3

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MYEKPRILPFGDKGIVVEFGNEITVECNEKVVNLYRILKKESRQGIISMIPTYRSLLVKYNPLQISYNELIKYVSEKMDK
EVEIKEEFKPQVYEIPVVYGGEFGPDLEFVAKHNNLSIEEVIAIHSAPLYRIYMVGFTMGFAYLGGMSEKIATPRLETPR
TEIKAGSVGIAGNQTGIYPLSSPGGWRIIGRTPVRLYDQEREKPILFEAGNYIKFIPVSEEEYYKIEKEIQEGKYQLRIY
EYLNHNLSG

Sequences:

>Translated_249_residues
MYEKPRILPFGDKGIVVEFGNEITVECNEKVVNLYRILKKESRQGIISMIPTYRSLLVKYNPLQISYNELIKYVSEKMDK
EVEIKEEFKPQVYEIPVVYGGEFGPDLEFVAKHNNLSIEEVIAIHSAPLYRIYMVGFTMGFAYLGGMSEKIATPRLETPR
TEIKAGSVGIAGNQTGIYPLSSPGGWRIIGRTPVRLYDQEREKPILFEAGNYIKFIPVSEEEYYKIEKEIQEGKYQLRIY
EYLNHNLSG
>Mature_249_residues
MYEKPRILPFGDKGIVVEFGNEITVECNEKVVNLYRILKKESRQGIISMIPTYRSLLVKYNPLQISYNELIKYVSEKMDK
EVEIKEEFKPQVYEIPVVYGGEFGPDLEFVAKHNNLSIEEVIAIHSAPLYRIYMVGFTMGFAYLGGMSEKIATPRLETPR
TEIKAGSVGIAGNQTGIYPLSSPGGWRIIGRTPVRLYDQEREKPILFEAGNYIKFIPVSEEEYYKIEKEIQEGKYQLRIY
EYLNHNLSG

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=221, Percent_Identity=35.7466063348416, Blast_Score=151, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6319685, Length=263, Percent_Identity=25.8555133079848, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: 3.5.1.54

Molecular weight: Translated: 28668; Mature: 28668

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEKPRILPFGDKGIVVEFGNEITVECNEKVVNLYRILKKESRQGIISMIPTYRSLLVKY
CCCCCCEEEECCCEEEEEECCEEEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEC
NPLQISYNELIKYVSEKMDKEVEIKEEFKPQVYEIPVVYGGEFGPDLEFVAKHNNLSIEE
CCEEECHHHHHHHHHHHCCCCEEEHHHCCCEEEEEEEEECCCCCCCCEEEEECCCCCHHH
VIAIHSAPLYRIYMVGFTMGFAYLGGMSEKIATPRLETPRTEIKAGSVGIAGNQTGIYPL
HHHHCCCCCEEHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHCCCCCEEECCCCCCEEEC
SSPGGWRIIGRTPVRLYDQEREKPILFEAGNYIKFIPVSEEEYYKIEKEIQEGKYQLRIY
CCCCCEEEEECCCEEEECCCCCCCEEEECCCEEEEEECCCHHHHHHHHHHHCCCEEEEEE
EYLNHNLSG
EHHCCCCCC
>Mature Secondary Structure
MYEKPRILPFGDKGIVVEFGNEITVECNEKVVNLYRILKKESRQGIISMIPTYRSLLVKY
CCCCCCEEEECCCEEEEEECCEEEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEC
NPLQISYNELIKYVSEKMDKEVEIKEEFKPQVYEIPVVYGGEFGPDLEFVAKHNNLSIEE
CCEEECHHHHHHHHHHHCCCCEEEHHHCCCEEEEEEEEECCCCCCCCEEEEECCCCCHHH
VIAIHSAPLYRIYMVGFTMGFAYLGGMSEKIATPRLETPRTEIKAGSVGIAGNQTGIYPL
HHHHCCCCCEEHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHCCCCCEEECCCCCCEEEC
SSPGGWRIIGRTPVRLYDQEREKPILFEAGNYIKFIPVSEEEYYKIEKEIQEGKYQLRIY
CCCCCEEEEECCCEEEECCCCCCCEEEECCCEEEEEECCCHHHHHHHHHHHCCCEEEEEE
EYLNHNLSG
EHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: urea-1-carboxylate; H2O

Specific reaction: urea-1-carboxylate + H2O = 2 CO2 + 2 NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]