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The map label for this gene is pyrB

Identifier: 20807970

GI number: 20807970

Start: 1497072

End: 1497986

Strand: Reverse

Name: pyrB

Synonym: TTE1534

Alternate gene names: 20807970

Gene position: 1497986-1497072 (Counterclockwise)

Preceding gene: 20807971

Following gene: 20807969

Centisome position: 55.7

GC content: 42.62

Gene sequence:

>915_bases
ATGCTCAAAAAGGACCTTCTGGGGATTAAAGACCTTACAAGAGAAGAAATACTGGAAATTCTGGATTTGGCCTTTGAGAT
GAAAAAGCTGTTAAACGGTGAAAAGTATTCCCAAGCCTTAAGAGGAAAAACTGTGGTGACAATCTTCTTCGAGCCCAGCA
CAAGGACAAGGTTGTCTTTTGAAATGGCTGCCAAATACCTAGGAGCCCATTATGGAAATATAGAGGTGGCTACCAGCAGT
GTAGTAAAGGGAGAGTCCCTCATAGATACTATAAGGACAGTTGAAAGAATGAAAGCAGATGTGATAGTGACAAGGCACAA
TATGAGTGGAACTCCTCACCTTCTTGCGAAATACACAAATGCTTCCATCATAAACGGAGGGGATGGCATAAATGAACATC
CAACACAGGCGCTTTTGGATATGATGACTATAAAGGAGAAAAAAGGGGATTTTAAAGGATTAAAAGTTGCTATAATAGGT
GATATTATGCACAGCAGGGTGGCAAGGTCTAACATATGGGGGCTTAAGAAATTGGGAGCAGAAGTGAAAGTGGCGGGACC
TTCCACACTAATGCCTCCTCAGATAGAGAAAATGGGGGTCAAAGTCTGCTATAACGCTGAAGAGGCTATAAAGGATGCAG
ATGTTGTGATGGGATTGAGAATTCAATTGGAAAGGCAAAAAAGCGGGCTATTTCCTTCAATTGAGGAGTACAGGGAGTAT
TTCGGAATAAATGAGGAGAGAATGAGACTGGCAAAATCTGACGCTATTTTGATGCACCCAGGTCCTATCAACAGGAATGT
GGAGGTTACCTCAGGTGCTGCAAATGCTAGTTACTCCGTAATAGAGGAGCAGATAACAAACGGGGTGGCTGTGAGGATGG
CACTTTTGAAAATTCTGTGTGAGAGGAGGAATTAG

Upstream 100 bases:

>100_bases
TTGATTTTTACAAAAAAGTATATTTTAAGAAATAAGAGTAGTTCCATTTCACCTTTTTGTGAAATGGGGACTACTCTTTT
TTTAAAGGAGGGAAAAGGTC

Downstream 100 bases:

>100_bases
AAATGAGGATCGTGATAAAAAATGGAATTGTGATTGATGGTTTTGGCGGTGAAGAAAAAGCTGACATCCTTATAAATTAC
GGCATAATAAAAGGGATTGA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MLKKDLLGIKDLTREEILEILDLAFEMKKLLNGEKYSQALRGKTVVTIFFEPSTRTRLSFEMAAKYLGAHYGNIEVATSS
VVKGESLIDTIRTVERMKADVIVTRHNMSGTPHLLAKYTNASIINGGDGINEHPTQALLDMMTIKEKKGDFKGLKVAIIG
DIMHSRVARSNIWGLKKLGAEVKVAGPSTLMPPQIEKMGVKVCYNAEEAIKDADVVMGLRIQLERQKSGLFPSIEEYREY
FGINEERMRLAKSDAILMHPGPINRNVEVTSGAANASYSVIEEQITNGVAVRMALLKILCERRN

Sequences:

>Translated_304_residues
MLKKDLLGIKDLTREEILEILDLAFEMKKLLNGEKYSQALRGKTVVTIFFEPSTRTRLSFEMAAKYLGAHYGNIEVATSS
VVKGESLIDTIRTVERMKADVIVTRHNMSGTPHLLAKYTNASIINGGDGINEHPTQALLDMMTIKEKKGDFKGLKVAIIG
DIMHSRVARSNIWGLKKLGAEVKVAGPSTLMPPQIEKMGVKVCYNAEEAIKDADVVMGLRIQLERQKSGLFPSIEEYREY
FGINEERMRLAKSDAILMHPGPINRNVEVTSGAANASYSVIEEQITNGVAVRMALLKILCERRN
>Mature_304_residues
MLKKDLLGIKDLTREEILEILDLAFEMKKLLNGEKYSQALRGKTVVTIFFEPSTRTRLSFEMAAKYLGAHYGNIEVATSS
VVKGESLIDTIRTVERMKADVIVTRHNMSGTPHLLAKYTNASIINGGDGINEHPTQALLDMMTIKEKKGDFKGLKVAIIG
DIMHSRVARSNIWGLKKLGAEVKVAGPSTLMPPQIEKMGVKVCYNAEEAIKDADVVMGLRIQLERQKSGLFPSIEEYREY
FGINEERMRLAKSDAILMHPGPINRNVEVTSGAANASYSVIEEQITNGVAVRMALLKILCERRN

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=314, Percent_Identity=35.9872611464968, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI38788445, Length=196, Percent_Identity=35.7142857142857, Blast_Score=85, Evalue=7e-17,
Organism=Escherichia coli, GI2367364, Length=313, Percent_Identity=30.9904153354633, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1786469, Length=288, Percent_Identity=28.125, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790703, Length=192, Percent_Identity=31.25, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=334, Percent_Identity=34.1317365269461, Blast_Score=130, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6322331, Length=308, Percent_Identity=34.7402597402597, Blast_Score=152, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6322373, Length=334, Percent_Identity=26.0479041916168, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24642586, Length=321, Percent_Identity=33.3333333333333, Blast_Score=149, Evalue=2e-36,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_THETN (Q8R9R5)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623141.1
- ProteinModelPortal:   Q8R9R5
- SMR:   Q8R9R5
- GeneID:   997968
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1534
- NMPDR:   fig|273068.3.peg.1488
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   TTEN273068:TTE1534-MONOMER
- BRENDA:   2.1.3.2
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 33822; Mature: 33822

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKDLLGIKDLTREEILEILDLAFEMKKLLNGEKYSQALRGKTVVTIFFEPSTRTRLSF
CCCHHHCCHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEEECCCCCCHHHH
EMAAKYLGAHYGNIEVATSSVVKGESLIDTIRTVERMKADVIVTRHNMSGTPHLLAKYTN
HHHHHHHCCCCCCEEEEHHHHHCCHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHEEECC
ASIINGGDGINEHPTQALLDMMTIKEKKGDFKGLKVAIIGDIMHSRVARSNIWGLKKLGA
CEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHCCCHHHCCC
EVKVAGPSTLMPPQIEKMGVKVCYNAEEAIKDADVVMGLRIQLERQKSGLFPSIEEYREY
EEEECCCCCCCCCCHHHCCEEEEECHHHHHHHHHHEEEEEEEEEHHHCCCCCCHHHHHHH
FGINEERMRLAKSDAILMHPGPINRNVEVTSGAANASYSVIEEQITNGVAVRMALLKILC
HCCCHHHHHHHHCCEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHCHHHHHHHHHHHHH
ERRN
HCCC
>Mature Secondary Structure
MLKKDLLGIKDLTREEILEILDLAFEMKKLLNGEKYSQALRGKTVVTIFFEPSTRTRLSF
CCCHHHCCHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEEECCCCCCHHHH
EMAAKYLGAHYGNIEVATSSVVKGESLIDTIRTVERMKADVIVTRHNMSGTPHLLAKYTN
HHHHHHHCCCCCCEEEEHHHHHCCHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHEEECC
ASIINGGDGINEHPTQALLDMMTIKEKKGDFKGLKVAIIGDIMHSRVARSNIWGLKKLGA
CEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHCCCHHHCCC
EVKVAGPSTLMPPQIEKMGVKVCYNAEEAIKDADVVMGLRIQLERQKSGLFPSIEEYREY
EEEECCCCCCCCCCHHHCCEEEEECHHHHHHHHHHEEEEEEEEEHHHCCCCCCHHHHHHH
FGINEERMRLAKSDAILMHPGPINRNVEVTSGAANASYSVIEEQITNGVAVRMALLKILC
HCCCHHHHHHHHCCEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHCHHHHHHHHHHHHH
ERRN
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336