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The map label for this gene is rlmI [H]

Identifier: 20807963

GI number: 20807963

Start: 1491126

End: 1492301

Strand: Direct

Name: rlmI [H]

Synonym: TTE1526

Alternate gene names: 20807963

Gene position: 1491126-1492301 (Clockwise)

Preceding gene: 20807961

Following gene: 20807964

Centisome position: 55.44

GC content: 36.9

Gene sequence:

>1176_bases
ATGGCAGACGTAATCTTAAGAAACATGAATATAAAGCGAATATTAGATGGACATCCTTGGGTATACAGAACTGAGATAGA
CAGGATTGAGGGAGATTACATCCCTGGAGATATTGTAAATGTGCTCACTCATAAAAAAGAATTTATTGGAAAAGGATACA
TAAACTTAAAATCGATGATATCCGTGCGAATTCTCACAAGAGACCCTGAAGAGGAAATAGGCGAAGAATTTTTTAGAAGG
CGAATTAAAAGAGCATGGGAATACAGAAAAAAAGTGATGGAAGATCTTAATTCGTGTCGAGTTGTATTTGGAGAAGGGGA
TTTTCTACCAGCATTGATTGTAGACAAATTTGGAGATTATCTTGTCATACAAACTCTTTCACTGGGTATAGACAAATACA
AAGAAACGATAGTGAAACTCCTCGTAGAAATATTAAATCCCAAAGGCATATTTGAAAGAAATGATGTCAACGTGAGGGAA
ATTGAAGGCCTACCTCAGCAAAAAGGATTTTTATACGGAAAGTTTGACCCTGTACAGCAGTTCAAAGAAAATGGCATTAA
ATTTTGGGTTGATATGGAAAATGGACAGAAGACAGGTTATTTTTTGGACCAAAAAGAAAATAGAAGAGCAATTCAAAATT
ATGTAAAAGGGGCCGAAGTACTGGATTGTTTTAGCCATACGGGTTCTTTTGCCGTTCACGCTCTTCACTATGGAGCAAAA
AGGGTAGAAATAGTGGATATATCTGAAGAAGCACTTGAAATGGCAAAAAAGAACGTAGAACTAAACGGCTATCAACAAAG
GGCTGAGTTTATAAGAGAGAATGCCTTTGACCTTTTGAGAAGGTACGACAGAGAAAAGAAAAAATTTGACACAGTAATAC
TGGACCCTCCTGCCTTTACAAAGAGCAAAGAAACAGTTAAAGACGCTTTAAGAGGATACAAAGAGATAAACTTAAGAGCT
TTAAAAATCATAAGAGAAGGGGGATTTCTCATCACCTGTTCCTGTTCTCAGCACATAAGACCCGATATGTTTTTAAATAC
AGTAAAAGAAGCAGCTTCTGACGCTAAAAGGACCATAAGACTTGTGGAACAAAGGACACAGGCAAAAGACCACCCAATAC
TGCTGGCTTCCGAAGAAACCCAATATCTTAAGTGCCTTATACTTCAGGTATTTTAA

Upstream 100 bases:

>100_bases
TAAATATTAATCCTGCTTTCTTTGCCATGTCAATATACAATTGCAAAATCAAGACATCTGTGGTATAAAAAACTTATCAA
AAACCGTAAGGAGAGGTGCT

Downstream 100 bases:

>100_bases
AGGAACACTCCCCTCATCTTATGCATACCCTGTAAAGAGGGAAAGTGCATAGATGAGGTGAAAAAATGGAAAGACAAGAA
TTGGGTTCTCGCCTTCTGTA

Product: SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MADVILRNMNIKRILDGHPWVYRTEIDRIEGDYIPGDIVNVLTHKKEFIGKGYINLKSMISVRILTRDPEEEIGEEFFRR
RIKRAWEYRKKVMEDLNSCRVVFGEGDFLPALIVDKFGDYLVIQTLSLGIDKYKETIVKLLVEILNPKGIFERNDVNVRE
IEGLPQQKGFLYGKFDPVQQFKENGIKFWVDMENGQKTGYFLDQKENRRAIQNYVKGAEVLDCFSHTGSFAVHALHYGAK
RVEIVDISEEALEMAKKNVELNGYQQRAEFIRENAFDLLRRYDREKKKFDTVILDPPAFTKSKETVKDALRGYKEINLRA
LKIIREGGFLITCSCSQHIRPDMFLNTVKEAASDAKRTIRLVEQRTQAKDHPILLASEETQYLKCLILQVF

Sequences:

>Translated_391_residues
MADVILRNMNIKRILDGHPWVYRTEIDRIEGDYIPGDIVNVLTHKKEFIGKGYINLKSMISVRILTRDPEEEIGEEFFRR
RIKRAWEYRKKVMEDLNSCRVVFGEGDFLPALIVDKFGDYLVIQTLSLGIDKYKETIVKLLVEILNPKGIFERNDVNVRE
IEGLPQQKGFLYGKFDPVQQFKENGIKFWVDMENGQKTGYFLDQKENRRAIQNYVKGAEVLDCFSHTGSFAVHALHYGAK
RVEIVDISEEALEMAKKNVELNGYQQRAEFIRENAFDLLRRYDREKKKFDTVILDPPAFTKSKETVKDALRGYKEINLRA
LKIIREGGFLITCSCSQHIRPDMFLNTVKEAASDAKRTIRLVEQRTQAKDHPILLASEETQYLKCLILQVF
>Mature_390_residues
ADVILRNMNIKRILDGHPWVYRTEIDRIEGDYIPGDIVNVLTHKKEFIGKGYINLKSMISVRILTRDPEEEIGEEFFRRR
IKRAWEYRKKVMEDLNSCRVVFGEGDFLPALIVDKFGDYLVIQTLSLGIDKYKETIVKLLVEILNPKGIFERNDVNVREI
EGLPQQKGFLYGKFDPVQQFKENGIKFWVDMENGQKTGYFLDQKENRRAIQNYVKGAEVLDCFSHTGSFAVHALHYGAKR
VEIVDISEEALEMAKKNVELNGYQQRAEFIRENAFDLLRRYDREKKKFDTVILDPPAFTKSKETVKDALRGYKEINLRAL
KIIREGGFLITCSCSQHIRPDMFLNTVKEAASDAKRTIRLVEQRTQAKDHPILLASEETQYLKCLILQVF

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=384, Percent_Identity=39.0625, Blast_Score=304, Evalue=6e-84,
Organism=Escherichia coli, GI1787181, Length=244, Percent_Identity=29.0983606557377, Blast_Score=92, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 45525; Mature: 45394

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADVILRNMNIKRILDGHPWVYRTEIDRIEGDYIPGDIVNVLTHKKEFIGKGYINLKSMI
CCCCEEECCCHHHHCCCCCEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEHHHHE
SVRILTRDPEEEIGEEFFRRRIKRAWEYRKKVMEDLNSCRVVFGEGDFLPALIVDKFGDY
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCE
LVIQTLSLGIDKYKETIVKLLVEILNPKGIFERNDVNVREIEGLPQQKGFLYGKFDPVQQ
EEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCEEECCCCHHHH
FKENGIKFWVDMENGQKTGYFLDQKENRRAIQNYVKGAEVLDCFSHTGSFAVHALHYGAK
HHHCCCEEEEECCCCCCCCEECCCCCHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHCCC
RVEIVDISEEALEMAKKNVELNGYQQRAEFIRENAFDLLRRYDREKKKFDTVILDPPAFT
EEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCC
KSKETVKDALRGYKEINLRALKIIREGGFLITCSCSQHIRPDMFLNTVKEAASDAKRTIR
CCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
LVEQRTQAKDHPILLASEETQYLKCLILQVF
HHHHHHCCCCCCEEEECCCHHHHHHHHHHCC
>Mature Secondary Structure 
ADVILRNMNIKRILDGHPWVYRTEIDRIEGDYIPGDIVNVLTHKKEFIGKGYINLKSMI
CCCEEECCCHHHHCCCCCEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEHHHHE
SVRILTRDPEEEIGEEFFRRRIKRAWEYRKKVMEDLNSCRVVFGEGDFLPALIVDKFGDY
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCE
LVIQTLSLGIDKYKETIVKLLVEILNPKGIFERNDVNVREIEGLPQQKGFLYGKFDPVQQ
EEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCEEECCCCHHHH
FKENGIKFWVDMENGQKTGYFLDQKENRRAIQNYVKGAEVLDCFSHTGSFAVHALHYGAK
HHHCCCEEEEECCCCCCCCEECCCCCHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHCCC
RVEIVDISEEALEMAKKNVELNGYQQRAEFIRENAFDLLRRYDREKKKFDTVILDPPAFT
EEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCC
KSKETVKDALRGYKEINLRALKIIREGGFLITCSCSQHIRPDMFLNTVKEAASDAKRTIR
CCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
LVEQRTQAKDHPILLASEETQYLKCLILQVF
HHHHHHCCCCCCEEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA