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Identifier: 20807950

GI number: 20807950

Start: 1472547

End: 1473026

Strand: Reverse

Name: def

Synonym: TTE1507

Alternate gene names: 20807950

Gene position: 1473026-1472547 (Counterclockwise)

Preceding gene: 20807951

Following gene: 20807949

Centisome position: 54.77

GC content: 42.29

Gene sequence:

>480_bases
TTGGCAATAAGGTATATTAGAAAAATTGGGGACCCCGTGCTCAGGAAAAAAGCTAAACCCGTTACTGAAATAAATTCTCA
CATCATTACTATTTTAGAAGATATGGCTGAGACCATGTATTTAAACGATGGGGTGGGCTTGGCAGCCAACCAGGTGGGGA
TTTTGAGAAGACTAGTCGTGATAGATGTGGGAGAAGGGCTTTTGGAGCTTATAAATCCTGAAATTGTGTACGAAGAGGGA
GAGCAGATCGGCCCTGAAGGATGTCTTAGCATTCCCGGAGTATTTGGGGAGGTAAAAAGGCCTCAAAAGGTTAAAGTAAG
GTATTTAGATAGAGAAGGAAATGTAAAAGAGATAGTAGGAGAAGACCTTCTGGCAAGGGCTTTATGCCACGAAATAGACC
ACCTAGAAGGCGTGCTATTTGTTGACAAAGTGATAAGATTCCTAGACAAAGAAGAGTTAGAAGGGGTCAAGGAGGTATGA

Upstream 100 bases:

>100_bases
AGAGTATGTCCAGAATATGAAATATCCAAAAGACATAAGGGTTGCTGTAGACATTGACCCACTTAATATAATGTAATTTA
AATTTAAGGAGGTATTTGTT

Downstream 100 bases:

>100_bases
GGTATTGAGGATTGTGTTCATGGGGACGCCAGAATTTGCCGTCCCCTCTTTAAAAAGGCTTTTTGAAGAAGGTTATGATG
TTCTGGCTGTGGTGACTCAG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MAIRYIRKIGDPVLRKKAKPVTEINSHIITILEDMAETMYLNDGVGLAANQVGILRRLVVIDVGEGLLELINPEIVYEEG
EQIGPEGCLSIPGVFGEVKRPQKVKVRYLDREGNVKEIVGEDLLARALCHEIDHLEGVLFVDKVIRFLDKEELEGVKEV

Sequences:

>Translated_159_residues
MAIRYIRKIGDPVLRKKAKPVTEINSHIITILEDMAETMYLNDGVGLAANQVGILRRLVVIDVGEGLLELINPEIVYEEG
EQIGPEGCLSIPGVFGEVKRPQKVKVRYLDREGNVKEIVGEDLLARALCHEIDHLEGVLFVDKVIRFLDKEELEGVKEV
>Mature_158_residues
AIRYIRKIGDPVLRKKAKPVTEINSHIITILEDMAETMYLNDGVGLAANQVGILRRLVVIDVGEGLLELINPEIVYEEGE
QIGPEGCLSIPGVFGEVKRPQKVKVRYLDREGNVKEIVGEDLLARALCHEIDHLEGVLFVDKVIRFLDKEELEGVKEV

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=163, Percent_Identity=28.2208588957055, Blast_Score=70, Evalue=6e-13,
Organism=Escherichia coli, GI1789682, Length=145, Percent_Identity=49.6551724137931, Blast_Score=124, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24645728, Length=165, Percent_Identity=36.3636363636364, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24645726, Length=167, Percent_Identity=32.9341317365269, Blast_Score=77, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_THETN (Q8R9T0)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623121.1
- ProteinModelPortal:   Q8R9T0
- SMR:   Q8R9T0
- GeneID:   995457
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1507
- NMPDR:   fig|273068.3.peg.1468
- HOGENOM:   HBG665227
- OMA:   YGEVERS
- ProtClustDB:   PRK00150
- BioCyc:   TTEN273068:TTE1507-MONOMER
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 17813; Mature: 17682

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIRYIRKIGDPVLRKKAKPVTEINSHIITILEDMAETMYLNDGVGLAANQVGILRRLVV
CHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
IDVGEGLLELINPEIVYEEGEQIGPEGCLSIPGVFGEVKRPQKVKVRYLDREGNVKEIVG
HHCCHHHHHHHCCHHEECCCCCCCCCCCCCCCCCHHCCCCCCEEEEEEECCCCCHHHHHH
EDLLARALCHEIDHLEGVLFVDKVIRFLDKEELEGVKEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCC
>Mature Secondary Structure 
AIRYIRKIGDPVLRKKAKPVTEINSHIITILEDMAETMYLNDGVGLAANQVGILRRLVV
HHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
IDVGEGLLELINPEIVYEEGEQIGPEGCLSIPGVFGEVKRPQKVKVRYLDREGNVKEIVG
HHCCHHHHHHHCCHHEECCCCCCCCCCCCCCCCCHHCCCCCCEEEEEEECCCCCHHHHHH
EDLLARALCHEIDHLEGVLFVDKVIRFLDKEELEGVKEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336