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The map label for this gene is Fmt

Identifier: 20807949

GI number: 20807949

Start: 1471613

End: 1472542

Strand: Reverse

Name: Fmt

Synonym: TTE1506

Alternate gene names: 20807949

Gene position: 1472542-1471613 (Counterclockwise)

Preceding gene: 20807950

Following gene: 20807948

Centisome position: 54.75

GC content: 41.18

Gene sequence:

>930_bases
TTGAGGATTGTGTTCATGGGGACGCCAGAATTTGCCGTCCCCTCTTTAAAAAGGCTTTTTGAAGAAGGTTATGATGTTCT
GGCTGTGGTGACTCAGCCGGACAAACAAAGAGGAAGAGGCATGAAAGTGAGTTTTTCTCCTGTAAAGGAATTAGCACTTC
AAAAAGGAGTGAAAGTGCTCCAACCTGAAAGCGTAAAAAATAATCCCGAGTTTTTGCAGGAATTAAAGGAATTAAATCCA
GAGGTTATTGTAGTAGCGGCTTATGGAAAAATTTTGCCTGAGGAGATTTTGACACTTCCTGAATACGGATGTATAAACGT
CCATGCATCGCTTTTGCCCAAGTACAGAGGAGCTGCTCCTATAAACTGGGCGATAATAAACGGAGAAAAAGAAACAGGAA
TTACCACTATGCTGATGGACAAAGGATTGGACACCGGAGACATGCTTTTAAAAAGGTCTATTGCCATTGAAGAGGATGAT
GATGCACAAACTTTACACGACAAGCTGGCCAATTTGGGGGCAGAAGTTTTGAGTGAGACCCTAAAGAAGCTAAAAGAAGG
GAAGTTGATTCCTGAAAAGCAAAAGGACGAAGAGGCAACTTACGCTCCGATTATAACTAAAGAGATGGGACACATTGACT
GGAAGAGCCCTGCTTGTAGGATTCGGAATTTGATAAGAGGGTTAAAACCTTGGCCTGGCTGCTATACTTTTTACGATGGC
AAGATGCTAAAAATCTGGAAGGCAGAAGTAGTAGAACATTTTGGGAATGAGCCTCCGGGAAGTGTGTTGAAGTCAAAAGA
TGAGCTTATTGTAAAGTGCGGGGAAAATGCTCTTAGAATCTTGGAAATCCAGCAAGAAGGTTCGAGAAAAATGGGTATTA
GAGAGTATTTAATAGGTCACAATATACCAGAAGGGACAATTTTAAAATAA

Upstream 100 bases:

>100_bases
TGCCACGAAATAGACCACCTAGAAGGCGTGCTATTTGTTGACAAAGTGATAAGATTCCTAGACAAAGAAGAGTTAGAAGG
GGTCAAGGAGGTATGAGGTA

Downstream 100 bases:

>100_bases
AAAAGAGGAGAATTTTTCTTGAAGGGGAAGAAGAGGGTTTTTCTGGGGATTTTAAGCTTTAGCTTTGTTTTTTTCGCGGT
GTTGCTAATAGCTTTTGTTT

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MRIVFMGTPEFAVPSLKRLFEEGYDVLAVVTQPDKQRGRGMKVSFSPVKELALQKGVKVLQPESVKNNPEFLQELKELNP
EVIVVAAYGKILPEEILTLPEYGCINVHASLLPKYRGAAPINWAIINGEKETGITTMLMDKGLDTGDMLLKRSIAIEEDD
DAQTLHDKLANLGAEVLSETLKKLKEGKLIPEKQKDEEATYAPIITKEMGHIDWKSPACRIRNLIRGLKPWPGCYTFYDG
KMLKIWKAEVVEHFGNEPPGSVLKSKDELIVKCGENALRILEIQQEGSRKMGIREYLIGHNIPEGTILK

Sequences:

>Translated_309_residues
MRIVFMGTPEFAVPSLKRLFEEGYDVLAVVTQPDKQRGRGMKVSFSPVKELALQKGVKVLQPESVKNNPEFLQELKELNP
EVIVVAAYGKILPEEILTLPEYGCINVHASLLPKYRGAAPINWAIINGEKETGITTMLMDKGLDTGDMLLKRSIAIEEDD
DAQTLHDKLANLGAEVLSETLKKLKEGKLIPEKQKDEEATYAPIITKEMGHIDWKSPACRIRNLIRGLKPWPGCYTFYDG
KMLKIWKAEVVEHFGNEPPGSVLKSKDELIVKCGENALRILEIQQEGSRKMGIREYLIGHNIPEGTILK
>Mature_309_residues
MRIVFMGTPEFAVPSLKRLFEEGYDVLAVVTQPDKQRGRGMKVSFSPVKELALQKGVKVLQPESVKNNPEFLQELKELNP
EVIVVAAYGKILPEEILTLPEYGCINVHASLLPKYRGAAPINWAIINGEKETGITTMLMDKGLDTGDMLLKRSIAIEEDD
DAQTLHDKLANLGAEVLSETLKKLKEGKLIPEKQKDEEATYAPIITKEMGHIDWKSPACRIRNLIRGLKPWPGCYTFYDG
KMLKIWKAEVVEHFGNEPPGSVLKSKDELIVKCGENALRILEIQQEGSRKMGIREYLIGHNIPEGTILK

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI21614513, Length=241, Percent_Identity=34.0248962655602, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI238814322, Length=313, Percent_Identity=28.7539936102236, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI164663775, Length=222, Percent_Identity=33.3333333333333, Blast_Score=101, Evalue=9e-22,
Organism=Escherichia coli, GI1789683, Length=298, Percent_Identity=44.9664429530201, Blast_Score=256, Evalue=1e-69,
Organism=Escherichia coli, GI1788589, Length=285, Percent_Identity=34.7368421052632, Blast_Score=188, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI133930964, Length=320, Percent_Identity=30.3125, Blast_Score=133, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6319458, Length=279, Percent_Identity=26.1648745519713, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45550868, Length=305, Percent_Identity=32.7868852459016, Blast_Score=134, Evalue=6e-32,
Organism=Drosophila melanogaster, GI28571984, Length=223, Percent_Identity=34.9775784753363, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24585660, Length=243, Percent_Identity=29.2181069958848, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_THETN (Q8R9T1)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623120.1
- ProteinModelPortal:   Q8R9T1
- SMR:   Q8R9T1
- GeneID:   997278
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1506
- NMPDR:   fig|273068.3.peg.1467
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- ProtClustDB:   CLSK901284
- BioCyc:   TTEN273068:TTE1506-MONOMER
- BRENDA:   2.1.2.9
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 34645; Mature: 34645

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIVFMGTPEFAVPSLKRLFEEGYDVLAVVTQPDKQRGRGMKVSFSPVKELALQKGVKVL
CEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCHHHHCCCCEEEHHHHHHHHHHCCCCEE
QPESVKNNPEFLQELKELNPEVIVVAAYGKILPEEILTLPEYGCINVHASLLPKYRGAAP
CCCCCCCCHHHHHHHHHCCCCEEEEEECCHHCHHHHHCCCCCCEEEEHHHHCCHHCCCCC
INWAIINGEKETGITTMLMDKGLDTGDMLLKRSIAIEEDDDAQTLHDKLANLGAEVLSET
EEEEEECCCCCCCCEEEHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
LKKLKEGKLIPEKQKDEEATYAPIITKEMGHIDWKSPACRIRNLIRGLKPWPGCYTFYDG
HHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEECC
KMLKIWKAEVVEHFGNEPPGSVLKSKDELIVKCGENALRILEIQQEGSRKMGIREYLIGH
CEEEHHHHHHHHHHCCCCCHHHHCCCCCEEEECCCCCEEEEEEHHCCCCCCCHHHHHHCC
NIPEGTILK
CCCCCCCCC
>Mature Secondary Structure
MRIVFMGTPEFAVPSLKRLFEEGYDVLAVVTQPDKQRGRGMKVSFSPVKELALQKGVKVL
CEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCHHHHCCCCEEEHHHHHHHHHHCCCCEE
QPESVKNNPEFLQELKELNPEVIVVAAYGKILPEEILTLPEYGCINVHASLLPKYRGAAP
CCCCCCCCHHHHHHHHHCCCCEEEEEECCHHCHHHHHCCCCCCEEEEHHHHCCHHCCCCC
INWAIINGEKETGITTMLMDKGLDTGDMLLKRSIAIEEDDDAQTLHDKLANLGAEVLSET
EEEEEECCCCCCCCEEEHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
LKKLKEGKLIPEKQKDEEATYAPIITKEMGHIDWKSPACRIRNLIRGLKPWPGCYTFYDG
HHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEECC
KMLKIWKAEVVEHFGNEPPGSVLKSKDELIVKCGENALRILEIQQEGSRKMGIREYLIGH
CEEEHHHHHHHHHHCCCCCHHHHCCCCCEEEECCCCCEEEEEEHHCCCCCCCHHHHHHCC
NIPEGTILK
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336