Click here to switch to the map view.

The map label for this gene is Thi80 [H]

Identifier: 20807940

GI number: 20807940

Start: 1462967

End: 1463602

Strand: Reverse

Name: Thi80 [H]

Synonym: TTE1497

Alternate gene names: 20807940

Gene position: 1463602-1462967 (Counterclockwise)

Preceding gene: 20807941

Following gene: 20807939

Centisome position: 54.42

GC content: 36.16

Gene sequence:

>636_bases
ATGAAAGTGCTGATCATTTCTAATGGAGAAATAAAAGATTATGGATTTTACAAAAACATTGTAGAAGAAGTAGATATGGT
CATATGCGCTGACGGAGGAGCTAATCACGCTTATAAGATGAAGATAAGGCCTTTTCTTATAATCGGGGATTTTGATTCTG
TAGACAGAGAAGTCCTTGAATTTTATCAAAAGGAAGGAGTTAAAGTAGAGAAGTTTCCTACAATGAAGGATGAGACAGAT
ACACAGCTTGCCTTGAAAAAAGCTATAGAGTTAGGAGCCAAAGACATAACTTTTATAGGAGTTATTGGCGACAGATTTGA
CCACTCCTATGCTAATCTTTCTCTTCTCCTCTATTCTTTAAAAAGAGGGGTAAAGAGCAGAATTATAAATGAAAAAAATG
AAATACACTTAATAGATGATTACATAGAGATTGAAGGGAAAAAAGGGGAATTGCTGTCTTTGCTTCCTTACTCGGGAGAA
GTGAAAGGAATATACACAAAAGGGCTTTTTTATCCTCTTTCCGGGCAGAGCATGAGCATGGAAAACCCTTACGGCATAAG
CAATGTTTTTACGGAGGAAAAAGCCAGCATAAAAATAGAAGAAGGGTTTTTGCTGGTCATAAAGCCTAAAGATTAG

Upstream 100 bases:

>100_bases
TCGTGGAAGCAGGCGCAGATATAATTGTTGCAGGGTCCGCCATTTTTGAGTCACCAGACCCTTCTTCTACTATAAAAGAG
TTCAGAGAGGCTGTAGGAAC

Downstream 100 bases:

>100_bases
TGTCTTTATCCCCTTCCTGTGAATAGTATGTATTAACTTCGAAAAGGAAGGGGCGTTTTTATGGGAAAACTCTTTCAAAA
ATTGAAAAAGTTAAACCGAC

Product: thiamine pyrophosphokinase

Products: NA

Alternate protein names: TPK; Thiamine diphosphokinase [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MKVLIISNGEIKDYGFYKNIVEEVDMVICADGGANHAYKMKIRPFLIIGDFDSVDREVLEFYQKEGVKVEKFPTMKDETD
TQLALKKAIELGAKDITFIGVIGDRFDHSYANLSLLLYSLKRGVKSRIINEKNEIHLIDDYIEIEGKKGELLSLLPYSGE
VKGIYTKGLFYPLSGQSMSMENPYGISNVFTEEKASIKIEEGFLLVIKPKD

Sequences:

>Translated_211_residues
MKVLIISNGEIKDYGFYKNIVEEVDMVICADGGANHAYKMKIRPFLIIGDFDSVDREVLEFYQKEGVKVEKFPTMKDETD
TQLALKKAIELGAKDITFIGVIGDRFDHSYANLSLLLYSLKRGVKSRIINEKNEIHLIDDYIEIEGKKGELLSLLPYSGE
VKGIYTKGLFYPLSGQSMSMENPYGISNVFTEEKASIKIEEGFLLVIKPKD
>Mature_211_residues
MKVLIISNGEIKDYGFYKNIVEEVDMVICADGGANHAYKMKIRPFLIIGDFDSVDREVLEFYQKEGVKVEKFPTMKDETD
TQLALKKAIELGAKDITFIGVIGDRFDHSYANLSLLLYSLKRGVKSRIINEKNEIHLIDDYIEIEGKKGELLSLLPYSGE
VKGIYTKGLFYPLSGQSMSMENPYGISNVFTEEKASIKIEEGFLLVIKPKD

Specific function: Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate [H]

COG id: COG1564

COG function: function code H; Thiamine pyrophosphokinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine pyrophosphokinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006282
- InterPro:   IPR007373
- InterPro:   IPR007371 [H]

Pfam domain/function: PF04263 TPK_catalytic [H]

EC number: =2.7.6.2 [H]

Molecular weight: Translated: 23878; Mature: 23878

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLIISNGEIKDYGFYKNIVEEVDMVICADGGANHAYKMKIRPFLIIGDFDSVDREVLE
CEEEEEECCCCCCCHHHHHHHHHCCEEEECCCCCCCEEEEEEEEEEEEECCCHHHHHHHH
FYQKEGVKVEKFPTMKDETDTQLALKKAIELGAKDITFIGVIGDRFDHSYANLSLLLYSL
HHHHCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHH
KRGVKSRIINEKNEIHLIDDYIEIEGKKGELLSLLPYSGEVKGIYTKGLFYPLSGQSMSM
HHHHHHHHCCCCCCEEEEEEEEEEECCCCCEEEECCCCCCEEEEEECCEEEECCCCCCCC
ENPYGISNVFTEEKASIKIEEGFLLVIKPKD
CCCCCCCHHCCCCCCEEEEECCEEEEEECCC
>Mature Secondary Structure
MKVLIISNGEIKDYGFYKNIVEEVDMVICADGGANHAYKMKIRPFLIIGDFDSVDREVLE
CEEEEEECCCCCCCHHHHHHHHHCCEEEECCCCCCCEEEEEEEEEEEEECCCHHHHHHHH
FYQKEGVKVEKFPTMKDETDTQLALKKAIELGAKDITFIGVIGDRFDHSYANLSLLLYSL
HHHHCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHH
KRGVKSRIINEKNEIHLIDDYIEIEGKKGELLSLLPYSGEVKGIYTKGLFYPLSGQSMSM
HHHHHHHHCCCCCCEEEEEEEEEEECCCCCEEEECCCCCCEEEEEECCEEEECCCCCCCC
ENPYGISNVFTEEKASIKIEEGFLLVIKPKD
CCCCCCCHHCCCCCCEEEEECCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377 [H]