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The map label for this gene is rnhB

Identifier: 20807898

GI number: 20807898

Start: 1426331

End: 1427056

Strand: Direct

Name: rnhB

Synonym: TTE1451

Alternate gene names: 20807898

Gene position: 1426331-1427056 (Clockwise)

Preceding gene: 20807812

Following gene: 20807899

Centisome position: 53.03

GC content: 37.33

Gene sequence:

>726_bases
GTGAAAAAGCTTAATTTGCGCCAGTTAAAAGAACACCTTTATCATGGTTCTCTTCAAGATTTGGACTTTTCAAATCTCAC
TTCTTCAGCAAAAGCATGGCTTGAAAGGGAAATAAAGAGAATAGAAGAAATGAAATTTTATGAAAAGAAGCTTTACGAAG
AAGGGCTAAATTTTATAGCAGGAGTTGATGAAGCAGGAAGAGGACCACTAGTGGGTCCTGTAGTAGCTGCTTGCGTAATA
CTCCCCAAAGAGATATTTATACCGGAGATAAACGACTCAAAAAAACTTCCTGAGGAAAAAAGAGAAAAATTGGCAGAAGT
GATAAAAAAAGAAGCGATTTCCTATGGAATAGGTATAGTAGACTGCAGAGAAATTGATGAGATAAACATACTAAATGCAA
CATTGAAAGCGATGAAGAAGGCTATCTTTGAAGTAAAAGAAAAAATAGAATACCTTCTGGTGGACGCCATCTCAATCCCC
GACATGCCTATAAAGCAGCTTCCCATAGTAAAAGGCGATTCAAAAAGCATATCAATTGCCGCTGCTTCAATTCTTGCAAA
AGTAGAAAGGGACAGGATTATGAGGGAATACCACAAACTTTACCCTCAATACAACTTTGCAAAAAACAAAGGATATGGCA
CAAAGGAACACATCGAAGCTTTAAAAAAGTACGGGCCATGTCCTATTCACAGGCGGACTTATGTAGAAAAAATTCTGAAA
GGATGA

Upstream 100 bases:

>100_bases
GTATCACCTTTTGTAAATTCTTTCTTCAATTTACCTTTATTATAATACAAAAACTGTGATAAAATAAAGGAGAACAAAAT
ATTAAAAAGGTGAGGAAGCC

Downstream 100 bases:

>100_bases
AAAAAGTGAACAAAAAAACAGTAGGCAGCGTAGGTGAAAAAATAGCTGCCCAGTATCTCTCTAAAAAGGGGTATAAAATT
TTAGAAAAAAACTTCAAGTG

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MKKLNLRQLKEHLYHGSLQDLDFSNLTSSAKAWLEREIKRIEEMKFYEKKLYEEGLNFIAGVDEAGRGPLVGPVVAACVI
LPKEIFIPEINDSKKLPEEKREKLAEVIKKEAISYGIGIVDCREIDEINILNATLKAMKKAIFEVKEKIEYLLVDAISIP
DMPIKQLPIVKGDSKSISIAAASILAKVERDRIMREYHKLYPQYNFAKNKGYGTKEHIEALKKYGPCPIHRRTYVEKILK
G

Sequences:

>Translated_241_residues
MKKLNLRQLKEHLYHGSLQDLDFSNLTSSAKAWLEREIKRIEEMKFYEKKLYEEGLNFIAGVDEAGRGPLVGPVVAACVI
LPKEIFIPEINDSKKLPEEKREKLAEVIKKEAISYGIGIVDCREIDEINILNATLKAMKKAIFEVKEKIEYLLVDAISIP
DMPIKQLPIVKGDSKSISIAAASILAKVERDRIMREYHKLYPQYNFAKNKGYGTKEHIEALKKYGPCPIHRRTYVEKILK
G
>Mature_241_residues
MKKLNLRQLKEHLYHGSLQDLDFSNLTSSAKAWLEREIKRIEEMKFYEKKLYEEGLNFIAGVDEAGRGPLVGPVVAACVI
LPKEIFIPEINDSKKLPEEKREKLAEVIKKEAISYGIGIVDCREIDEINILNATLKAMKKAIFEVKEKIEYLLVDAISIP
DMPIKQLPIVKGDSKSISIAAASILAKVERDRIMREYHKLYPQYNFAKNKGYGTKEHIEALKKYGPCPIHRRTYVEKILK
G

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=178, Percent_Identity=47.752808988764, Blast_Score=163, Evalue=8e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_THETN (Q8R9X9)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623069.1
- ProteinModelPortal:   Q8R9X9
- SMR:   Q8R9X9
- GeneID:   997892
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1451
- NMPDR:   fig|273068.3.peg.1416
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- BioCyc:   TTEN273068:TTE1451-MONOMER
- BRENDA:   3.1.26.4
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 27586; Mature: 27586

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLNLRQLKEHLYHGSLQDLDFSNLTSSAKAWLEREIKRIEEMKFYEKKLYEEGLNFIA
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVDEAGRGPLVGPVVAACVILPKEIFIPEINDSKKLPEEKREKLAEVIKKEAISYGIGIV
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
DCREIDEINILNATLKAMKKAIFEVKEKIEYLLVDAISIPDMPIKQLPIVKGDSKSISIA
CHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCHHHH
AASILAKVERDRIMREYHKLYPQYNFAKNKGYGTKEHIEALKKYGPCPIHRRTYVEKILK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHC
G
C
>Mature Secondary Structure
MKKLNLRQLKEHLYHGSLQDLDFSNLTSSAKAWLEREIKRIEEMKFYEKKLYEEGLNFIA
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVDEAGRGPLVGPVVAACVILPKEIFIPEINDSKKLPEEKREKLAEVIKKEAISYGIGIV
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
DCREIDEINILNATLKAMKKAIFEVKEKIEYLLVDAISIPDMPIKQLPIVKGDSKSISIA
CHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCHHHH
AASILAKVERDRIMREYHKLYPQYNFAKNKGYGTKEHIEALKKYGPCPIHRRTYVEKILK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336