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The map label for this gene is TopA [H]

Identifier: 20807896

GI number: 20807896

Start: 1423005

End: 1425089

Strand: Reverse

Name: TopA [H]

Synonym: TTE1449

Alternate gene names: 20807896

Gene position: 1425089-1423005 (Counterclockwise)

Preceding gene: 20807897

Following gene: 20807895

Centisome position: 52.99

GC content: 39.81

Gene sequence:

>2085_bases
ATGGCAAAGTCTCTAGTTATTGTGGAATCTCCTGCGAAGGCTAAAACTATTTCAAAATTTCTTGGAAAAAATTTTAAAGT
TGCTGCATCTCAAGGGCATGTCAGGGATTTGCCTAAAAGCCAGCTGGGCGTTGACATTGAAAATAACTTTGCTCCCAAGT
ACATAACAATAAGGGGCAAAGGCGAGATAATTGAAAAATTAAAAAAAGAGGCAAAAGATGCTGAGAAAATTTTCCTCGCT
ACAGACCCTGATAGAGAAGGAGAGGCAATTTCATGGCACATTGCCCAGCTTTTAGATTTGGATATTGACAAACCCTGTCG
CATAGAATTTCATGAAATTACAAAAAACGCAGTCCAAGCTGCTATAAAAAATCCGAGGAAAATAGACCTCAATTTAGTAA
ATGCTCAACAAGCGAGGAGAATCTTAGATAGGCTGGTAGGATATAAAATTAGCCCTATTCTATGGAAAAAGATAAAAAGG
GGGTTAAGTGCTGGAAGAGTTCAGTCAGTTGCTGCAAGACTGGTCTGTGACAGAGAAAGGGAGATAGAGGCCTTTGTGCC
TGAAGAATACTGGAATATAACTGCAGTTCTTTTTGACGAAAAAACTTCTAGCAAATTTGAGGCGAAGTTCTACGGAAGAG
AAGAAGAAAAGATTGAGCTAAAAAATAGAGAAGAAGTAGATAAGATAATAAATTCTCTATCTGAAGAATATGTCGTATAT
AAAGTAAAGACAGGGACAAAAAAGAGAAATCCGCCGCCTCCTTTCATCACTAGCACCTTACAACAAGAGGCATCGCGCAA
ACTGGGCTTTACTGCAAAGAAAACCATGTTAATTGCTCAGCAGCTTTACGAAGGAATTGAAATAAAAGGGGAAGGCAGTG
TAGGACTTGTCACTTATATAAGGACAGATTCTACAAGGGTAGCGAAAGAGGCAAAGGAAGAGGCATACAGGTATATCACA
GAGAAGTTTGGAAAAGAGTATGCAAATCCTGATGCAGAGTATTCTTCAAAAAAGGCCAATGTGCAGGATGCCCATGAGGC
TATAAGGCCTACTTCTGTGTTCAGAGAACCAGAGATTATAAAGGACTCTTTGACACCTGACCAGTACAAACTGTACAAAT
TGATATGGGATAGATTTATTGCAAGTCAAATGATGCCAGCAATTTATGACACAGTGGCTATGGACATTAAAAATAACGGC
TATATCTTCAAAGCTTCAGGGTCAAGCGTTAAATTCTTGGGGTTTATGACTGTCTATGTGGAAGGCAGTGACTTTCAGGA
AGAAGAGGAAGAAAAGACACTACCTGTATTAAAAGAAGGCACAAAAGTCAGATTGGAAGAGCTGAAGCCGACACAGCACT
TTACACAGCCGCCTCCCAGATACACTGAAGCCTCTCTCATTAAAGAACTAGAAGAAAAAGGAATAGGAAGACCTAGCACT
TATGCTCCAACTATCGCTACTCTTTTAGAGAGAGGATATGTAGTGAAGGACAAAAAAGTTTTAAAACCTACAGAGCTGGG
GTTTATCGTCACAGATGCCTTAAAAGAATTCTTCTCAGAAATTGTAGATGTGGGATTTACTGCTGAGATGGAGGAACAGC
TGGACAAGATAGAAGAAGGAAAAGTAGAATGGTATGAGGTGGTAGGAGACTTCTACAGAGATTTTTATAAGACTTTGAAG
GTTGCCGAAGAACAGATGGAGAAAGTGGAACTAGAAGAGGAAGTGGAAGTAACTGATATAAAGTGTGAAGTTTGCGGAAG
GCCGATGGTAGTCAGAAAGGGTAGATACGGCAAATTCTTGGCCTGTTCAGGGTTTCCAGAGTGCAAAAACACAAAACCCC
TTCTTGAAAAAGTGGGTGTAAAATGCCCTAAATGTGGAGGGGAAATTGTAAAGAGAAGGAGCAAAAAAGGAAGGGTTTAT
TATTCATGTGAAAATGCGCCTCAGTGCGATTTTGTGCTTTGGGATAAGCCGGTCAATGAAAAGTGTCCGGTTTGTGGCAG
CCTAATGGTAGAAAAAAATACGAAAAACGGCATTGTCATAAAGTGTTCTAATCCAGAGTGCGGATATCAGAAAGAAACTA
ATTAA

Upstream 100 bases:

>100_bases
GTTTGATTGACAGGCTCCCTGGCAACAAATATACTAAAAAAATTTTTTGATAATTACAAAAAACTTTGATATAATTATTA
GTTGTTTTGGAGGTGTACTC

Downstream 100 bases:

>100_bases
ATGAATTTATCAAAAATTTTGAATTAATCTTGAAGCTTTTCACAAAATGTGTTATAAATGTTTAAAGGGGTGTAATTATG
TTTAGAGGCACTACTATAGT

Product: topoisomerase IA

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 694; Mature: 693

Protein sequence:

>694_residues
MAKSLVIVESPAKAKTISKFLGKNFKVAASQGHVRDLPKSQLGVDIENNFAPKYITIRGKGEIIEKLKKEAKDAEKIFLA
TDPDREGEAISWHIAQLLDLDIDKPCRIEFHEITKNAVQAAIKNPRKIDLNLVNAQQARRILDRLVGYKISPILWKKIKR
GLSAGRVQSVAARLVCDREREIEAFVPEEYWNITAVLFDEKTSSKFEAKFYGREEEKIELKNREEVDKIINSLSEEYVVY
KVKTGTKKRNPPPPFITSTLQQEASRKLGFTAKKTMLIAQQLYEGIEIKGEGSVGLVTYIRTDSTRVAKEAKEEAYRYIT
EKFGKEYANPDAEYSSKKANVQDAHEAIRPTSVFREPEIIKDSLTPDQYKLYKLIWDRFIASQMMPAIYDTVAMDIKNNG
YIFKASGSSVKFLGFMTVYVEGSDFQEEEEEKTLPVLKEGTKVRLEELKPTQHFTQPPPRYTEASLIKELEEKGIGRPST
YAPTIATLLERGYVVKDKKVLKPTELGFIVTDALKEFFSEIVDVGFTAEMEEQLDKIEEGKVEWYEVVGDFYRDFYKTLK
VAEEQMEKVELEEEVEVTDIKCEVCGRPMVVRKGRYGKFLACSGFPECKNTKPLLEKVGVKCPKCGGEIVKRRSKKGRVY
YSCENAPQCDFVLWDKPVNEKCPVCGSLMVEKNTKNGIVIKCSNPECGYQKETN

Sequences:

>Translated_694_residues
MAKSLVIVESPAKAKTISKFLGKNFKVAASQGHVRDLPKSQLGVDIENNFAPKYITIRGKGEIIEKLKKEAKDAEKIFLA
TDPDREGEAISWHIAQLLDLDIDKPCRIEFHEITKNAVQAAIKNPRKIDLNLVNAQQARRILDRLVGYKISPILWKKIKR
GLSAGRVQSVAARLVCDREREIEAFVPEEYWNITAVLFDEKTSSKFEAKFYGREEEKIELKNREEVDKIINSLSEEYVVY
KVKTGTKKRNPPPPFITSTLQQEASRKLGFTAKKTMLIAQQLYEGIEIKGEGSVGLVTYIRTDSTRVAKEAKEEAYRYIT
EKFGKEYANPDAEYSSKKANVQDAHEAIRPTSVFREPEIIKDSLTPDQYKLYKLIWDRFIASQMMPAIYDTVAMDIKNNG
YIFKASGSSVKFLGFMTVYVEGSDFQEEEEEKTLPVLKEGTKVRLEELKPTQHFTQPPPRYTEASLIKELEEKGIGRPST
YAPTIATLLERGYVVKDKKVLKPTELGFIVTDALKEFFSEIVDVGFTAEMEEQLDKIEEGKVEWYEVVGDFYRDFYKTLK
VAEEQMEKVELEEEVEVTDIKCEVCGRPMVVRKGRYGKFLACSGFPECKNTKPLLEKVGVKCPKCGGEIVKRRSKKGRVY
YSCENAPQCDFVLWDKPVNEKCPVCGSLMVEKNTKNGIVIKCSNPECGYQKETN
>Mature_693_residues
AKSLVIVESPAKAKTISKFLGKNFKVAASQGHVRDLPKSQLGVDIENNFAPKYITIRGKGEIIEKLKKEAKDAEKIFLAT
DPDREGEAISWHIAQLLDLDIDKPCRIEFHEITKNAVQAAIKNPRKIDLNLVNAQQARRILDRLVGYKISPILWKKIKRG
LSAGRVQSVAARLVCDREREIEAFVPEEYWNITAVLFDEKTSSKFEAKFYGREEEKIELKNREEVDKIINSLSEEYVVYK
VKTGTKKRNPPPPFITSTLQQEASRKLGFTAKKTMLIAQQLYEGIEIKGEGSVGLVTYIRTDSTRVAKEAKEEAYRYITE
KFGKEYANPDAEYSSKKANVQDAHEAIRPTSVFREPEIIKDSLTPDQYKLYKLIWDRFIASQMMPAIYDTVAMDIKNNGY
IFKASGSSVKFLGFMTVYVEGSDFQEEEEEKTLPVLKEGTKVRLEELKPTQHFTQPPPRYTEASLIKELEEKGIGRPSTY
APTIATLLERGYVVKDKKVLKPTELGFIVTDALKEFFSEIVDVGFTAEMEEQLDKIEEGKVEWYEVVGDFYRDFYKTLKV
AEEQMEKVELEEEVEVTDIKCEVCGRPMVVRKGRYGKFLACSGFPECKNTKPLLEKVGVKCPKCGGEIVKRRSKKGRVYY
SCENAPQCDFVLWDKPVNEKCPVCGSLMVEKNTKNGIVIKCSNPECGYQKETN

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=628, Percent_Identity=25.3184713375796, Blast_Score=137, Evalue=4e-32,
Organism=Homo sapiens, GI4507635, Length=665, Percent_Identity=23.7593984962406, Blast_Score=128, Evalue=2e-29,
Organism=Escherichia coli, GI1787529, Length=754, Percent_Identity=40.053050397878, Blast_Score=526, Evalue=1e-150,
Organism=Escherichia coli, GI1788061, Length=554, Percent_Identity=24.7292418772563, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI48994931, Length=113, Percent_Identity=38.0530973451327, Blast_Score=99, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI32563869, Length=722, Percent_Identity=24.3767313019391, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17555378, Length=560, Percent_Identity=26.0714285714286, Blast_Score=125, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6323263, Length=576, Percent_Identity=24.3055555555556, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24585251, Length=647, Percent_Identity=24.7295208655332, Blast_Score=133, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24640096, Length=502, Percent_Identity=21.9123505976096, Blast_Score=102, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 79056; Mature: 78925

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSLVIVESPAKAKTISKFLGKNFKVAASQGHVRDLPKSQLGVDIENNFAPKYITIRGK
CCCCEEEEECCCHHHHHHHHHCCCEEEEECCCCCCCCCHHHCCCEECCCCCCCEEEEECC
GEIIEKLKKEAKDAEKIFLATDPDREGEAISWHIAQLLDLDIDKPCRIEFHEITKNAVQA
HHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
AIKNPRKIDLNLVNAQQARRILDRLVGYKISPILWKKIKRGLSAGRVQSVAARLVCDRER
HHCCCCEEEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
EIEAFVPEEYWNITAVLFDEKTSSKFEAKFYGREEEKIELKNREEVDKIINSLSEEYVVY
CCHHCCCHHHCCEEEEEEECCCCCCEEEEECCCCCHHCCCCCHHHHHHHHHHCCCCEEEE
KVKTGTKKRNPPPPFITSTLQQEASRKLGFTAKKTMLIAQQLYEGIEIKGEGSVGLVTYI
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEECCCCEEEEEEE
RTDSTRVAKEAKEEAYRYITEKFGKEYANPDAEYSSKKANVQDAHEAIRPTSVFREPEII
ECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHCCCHHH
KDSLTPDQYKLYKLIWDRFIASQMMPAIYDTVAMDIKNNGYIFKASGSSVKFLGFMTVYV
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCCEEEEEEEEEEE
EGSDFQEEEEEKTLPVLKEGTKVRLEELKPTQHFTQPPPRYTEASLIKELEEKGIGRPST
ECCCCCCHHHHCCCCHHHCCCEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
YAPTIATLLERGYVVKDKKVLKPTELGFIVTDALKEFFSEIVDVGFTAEMEEQLDKIEEG
CHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
KVEWYEVVGDFYRDFYKTLKVAEEQMEKVELEEEVEVTDIKCEVCGRPMVVRKGRYGKFL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCEEEEEEEECCCCEEEECCCCCCEE
ACSGFPECKNTKPLLEKVGVKCPKCGGEIVKRRSKKGRVYYSCENAPQCDFVLWDKPVNE
EECCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCCC
KCPVCGSLMVEKNTKNGIVIKCSNPECGYQKETN
CCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
AKSLVIVESPAKAKTISKFLGKNFKVAASQGHVRDLPKSQLGVDIENNFAPKYITIRGK
CCCEEEEECCCHHHHHHHHHCCCEEEEECCCCCCCCCHHHCCCEECCCCCCCEEEEECC
GEIIEKLKKEAKDAEKIFLATDPDREGEAISWHIAQLLDLDIDKPCRIEFHEITKNAVQA
HHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
AIKNPRKIDLNLVNAQQARRILDRLVGYKISPILWKKIKRGLSAGRVQSVAARLVCDRER
HHCCCCEEEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
EIEAFVPEEYWNITAVLFDEKTSSKFEAKFYGREEEKIELKNREEVDKIINSLSEEYVVY
CCHHCCCHHHCCEEEEEEECCCCCCEEEEECCCCCHHCCCCCHHHHHHHHHHCCCCEEEE
KVKTGTKKRNPPPPFITSTLQQEASRKLGFTAKKTMLIAQQLYEGIEIKGEGSVGLVTYI
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEECCCCEEEEEEE
RTDSTRVAKEAKEEAYRYITEKFGKEYANPDAEYSSKKANVQDAHEAIRPTSVFREPEII
ECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHCCCHHH
KDSLTPDQYKLYKLIWDRFIASQMMPAIYDTVAMDIKNNGYIFKASGSSVKFLGFMTVYV
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCCEEEEEEEEEEE
EGSDFQEEEEEKTLPVLKEGTKVRLEELKPTQHFTQPPPRYTEASLIKELEEKGIGRPST
ECCCCCCHHHHCCCCHHHCCCEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
YAPTIATLLERGYVVKDKKVLKPTELGFIVTDALKEFFSEIVDVGFTAEMEEQLDKIEEG
CHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
KVEWYEVVGDFYRDFYKTLKVAEEQMEKVELEEEVEVTDIKCEVCGRPMVVRKGRYGKFL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCEEEEEEEECCCCEEEECCCCCCEE
ACSGFPECKNTKPLLEKVGVKCPKCGGEIVKRRSKKGRVYYSCENAPQCDFVLWDKPVNE
EECCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCCC
KCPVCGSLMVEKNTKNGIVIKCSNPECGYQKETN
CCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]