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The map label for this gene is PqqL [H]

Identifier: 20807832

GI number: 20807832

Start: 1367629

End: 1368891

Strand: Reverse

Name: PqqL [H]

Synonym: TTE1385

Alternate gene names: 20807832

Gene position: 1368891-1367629 (Counterclockwise)

Preceding gene: 20807833

Following gene: 20807831

Centisome position: 50.9

GC content: 36.1

Gene sequence:

>1263_bases
ATGAAGTTGTATCAACAGAAGATGATAGCTGGAGTGAAAGTTGCAAGTTGCAAAATACCTTATGCTCATTCAGTTTACGT
AGGTATTTGGATAAAAGCAGGCTCAATGTACGAAACCAAAAACATAAATGGTATATCTCACTTTATAGAACACCTTGTGT
TTAAAGGGTCTAACTTGCGAAGCGCAAGGCAGATAGCTGAAGAGATGGACAGCATAGGTGGACAGCTAAACGGCTTTACT
GAAAAAGAAGACACTTGCTTTTATATAAAAGTTTTAAATAGCCATATAAAAAAAGGCATTGACATTCTCTTTGACATGGT
TTTTAATCCTGCGTTTTGCGAAGAAGACATTTATAAAGAAAAACAGGTGGTCTTTGAAGAAATATTGACAGAACTTGATT
CACCTGAAGACGTAGCTTACAACCTGCTTGCCAAAACAGCCTGGAGAGGACATTCTCTATCCCTGCCCGTGCTGGGAACA
TTTACTACAATTAAGAATTTGAGCAAAAACCATATACTAGAATACTATGAGAGGCACTACACTAAAGACAACATAGTAGT
ATCTATAGCGGGAAATTTTGACGATGAAATATTTGAAGTGTTGGAAGGCTATCTTTCAAAAATTAAGCCAACTACTTCTA
ATTTTTCTCTTATCCCTCCCTTATGGCACAAAGATGTCTCTCTTTACGAAAAAGATTTTGAGCAGGTTAACCTCTGCATC
GGCCTCCCTGGAATACCCTATGATTTAAAGAAGGTATATGCTCTTGCAATTGCAAACAATGCTTTTGGTGGGGGCATGAG
TTCAAGACTTTTTCAAAAAATAAGAGAAGACAAGGGCTTAGTCTATTCCATCTATTCATACCCTGCAACTTATCCGACCG
GAGGAATGTTTACAATTTTTGCAAGTATGACTCCGAGCAATTTTAGAAAAGTCTATGACTTGATAATAAAAGAAATTGAA
GAAATTTCAAAAAAAGGCCTTACTAAAGAGGAATTTGACAAGTTTAAAGAACAACTCAAAATAAATATCTTGATGGACCA
AGACAGCATAAGCACCAGAATGAGCAGTATAGGAAAATCCCTTCTACTTTTTGATAAAGTGCATCTTATTGAGGATGTTT
TGAAGATAGTGGAGGAAATATCTTTTGAGGAAGTAAACCAGCTGGCAAAAGAGATAATAAGGCCAGAAGAAATGACTGTA
TCTGTCGTGGGTAAACTTAATAAAAAAGATAAAAGGTGGTTGGAAAATGTCAATAGTGCTTAA

Upstream 100 bases:

>100_bases
GTTGAAAGAAAAGGGTTTTAAAATAACTAAAGTTTCAGAGGTGATTGTAGACAAAAAATAAATTTGCTATAATAATAGTA
AATTTTTTAAGACAGAGGGG

Downstream 100 bases:

>100_bases
GATAAAGAGAACAGAAGATGCAAAAGATTTACCTTTACCCGCTTATATGAGCGAAGGAGCTGCGGGAATGGACTTATACG
CCAATGTAAAGGGTGAGGTA

Product: Zn-dependent peptidase

Products: NA

Alternate protein names: ORFP [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MKLYQQKMIAGVKVASCKIPYAHSVYVGIWIKAGSMYETKNINGISHFIEHLVFKGSNLRSARQIAEEMDSIGGQLNGFT
EKEDTCFYIKVLNSHIKKGIDILFDMVFNPAFCEEDIYKEKQVVFEEILTELDSPEDVAYNLLAKTAWRGHSLSLPVLGT
FTTIKNLSKNHILEYYERHYTKDNIVVSIAGNFDDEIFEVLEGYLSKIKPTTSNFSLIPPLWHKDVSLYEKDFEQVNLCI
GLPGIPYDLKKVYALAIANNAFGGGMSSRLFQKIREDKGLVYSIYSYPATYPTGGMFTIFASMTPSNFRKVYDLIIKEIE
EISKKGLTKEEFDKFKEQLKINILMDQDSISTRMSSIGKSLLLFDKVHLIEDVLKIVEEISFEEVNQLAKEIIRPEEMTV
SVVGKLNKKDKRWLENVNSA

Sequences:

>Translated_420_residues
MKLYQQKMIAGVKVASCKIPYAHSVYVGIWIKAGSMYETKNINGISHFIEHLVFKGSNLRSARQIAEEMDSIGGQLNGFT
EKEDTCFYIKVLNSHIKKGIDILFDMVFNPAFCEEDIYKEKQVVFEEILTELDSPEDVAYNLLAKTAWRGHSLSLPVLGT
FTTIKNLSKNHILEYYERHYTKDNIVVSIAGNFDDEIFEVLEGYLSKIKPTTSNFSLIPPLWHKDVSLYEKDFEQVNLCI
GLPGIPYDLKKVYALAIANNAFGGGMSSRLFQKIREDKGLVYSIYSYPATYPTGGMFTIFASMTPSNFRKVYDLIIKEIE
EISKKGLTKEEFDKFKEQLKINILMDQDSISTRMSSIGKSLLLFDKVHLIEDVLKIVEEISFEEVNQLAKEIIRPEEMTV
SVVGKLNKKDKRWLENVNSA
>Mature_420_residues
MKLYQQKMIAGVKVASCKIPYAHSVYVGIWIKAGSMYETKNINGISHFIEHLVFKGSNLRSARQIAEEMDSIGGQLNGFT
EKEDTCFYIKVLNSHIKKGIDILFDMVFNPAFCEEDIYKEKQVVFEEILTELDSPEDVAYNLLAKTAWRGHSLSLPVLGT
FTTIKNLSKNHILEYYERHYTKDNIVVSIAGNFDDEIFEVLEGYLSKIKPTTSNFSLIPPLWHKDVSLYEKDFEQVNLCI
GLPGIPYDLKKVYALAIANNAFGGGMSSRLFQKIREDKGLVYSIYSYPATYPTGGMFTIFASMTPSNFRKVYDLIIKEIE
EISKKGLTKEEFDKFKEQLKINILMDQDSISTRMSSIGKSLLLFDKVHLIEDVLKIVEEISFEEVNQLAKEIIRPEEMTV
SVVGKLNKKDKRWLENVNSA

Specific function: Unknown

COG id: COG0612

COG function: function code R; Predicted Zn-dependent peptidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M16 family [H]

Homologues:

Organism=Homo sapiens, GI94538354, Length=412, Percent_Identity=24.5145631067961, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI46593007, Length=420, Percent_Identity=25.7142857142857, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI24308013, Length=436, Percent_Identity=22.9357798165138, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1787770, Length=204, Percent_Identity=23.5294117647059, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71999683, Length=393, Percent_Identity=24.1730279898219, Blast_Score=157, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI17553678, Length=402, Percent_Identity=24.3781094527363, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17510601, Length=376, Percent_Identity=19.9468085106383, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323192, Length=399, Percent_Identity=26.0651629072682, Blast_Score=146, Evalue=8e-36,
Organism=Saccharomyces cerevisiae, GI6321813, Length=407, Percent_Identity=25.3071253071253, Blast_Score=140, Evalue=3e-34,
Organism=Drosophila melanogaster, GI21357875, Length=409, Percent_Identity=25.6723716381418, Blast_Score=154, Evalue=8e-38,
Organism=Drosophila melanogaster, GI24646943, Length=409, Percent_Identity=25.6723716381418, Blast_Score=154, Evalue=8e-38,
Organism=Drosophila melanogaster, GI19921772, Length=417, Percent_Identity=22.3021582733813, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24667786, Length=188, Percent_Identity=27.1276595744681, Blast_Score=75, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011249
- InterPro:   IPR011237
- InterPro:   IPR011765
- InterPro:   IPR001431
- InterPro:   IPR007863 [H]

Pfam domain/function: PF00675 Peptidase_M16; PF05193 Peptidase_M16_C [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 47956; Mature: 47956

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00143 INSULINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYQQKMIAGVKVASCKIPYAHSVYVGIWIKAGSMYETKNINGISHFIEHLVFKGSNLR
CCHHHHHHHHCCEEEEEECCCCCEEEEEEEEECCCEECCCCCCHHHHHHHHHHHCCCCHH
SARQIAEEMDSIGGQLNGFTEKEDTCFYIKVLNSHIKKGIDILFDMVFNPAFCEEDIYKE
HHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
KQVVFEEILTELDSPEDVAYNLLAKTAWRGHSLSLPVLGTFTTIKNLSKNHILEYYERHY
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHC
TKDNIVVSIAGNFDDEIFEVLEGYLSKIKPTTSNFSLIPPLWHKDVSLYEKDFEQVNLCI
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHEEEEE
GLPGIPYDLKKVYALAIANNAFGGGMSSRLFQKIREDKGLVYSIYSYPATYPTGGMFTIF
ECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEE
ASMTPSNFRKVYDLIIKEIEEISKKGLTKEEFDKFKEQLKINILMDQDSISTRMSSIGKS
ECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEEEECCHHHHHHHHHHCHH
LLLFDKVHLIEDVLKIVEEISFEEVNQLAKEIIRPEEMTVSVVGKLNKKDKRWLENVNSA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKLYQQKMIAGVKVASCKIPYAHSVYVGIWIKAGSMYETKNINGISHFIEHLVFKGSNLR
CCHHHHHHHHCCEEEEEECCCCCEEEEEEEEECCCEECCCCCCHHHHHHHHHHHCCCCHH
SARQIAEEMDSIGGQLNGFTEKEDTCFYIKVLNSHIKKGIDILFDMVFNPAFCEEDIYKE
HHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
KQVVFEEILTELDSPEDVAYNLLAKTAWRGHSLSLPVLGTFTTIKNLSKNHILEYYERHY
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHC
TKDNIVVSIAGNFDDEIFEVLEGYLSKIKPTTSNFSLIPPLWHKDVSLYEKDFEQVNLCI
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHEEEEE
GLPGIPYDLKKVYALAIANNAFGGGMSSRLFQKIREDKGLVYSIYSYPATYPTGGMFTIF
ECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEE
ASMTPSNFRKVYDLIIKEIEEISKKGLTKEEFDKFKEQLKINILMDQDSISTRMSSIGKS
ECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEEEECCHHHHHHHHHHCHH
LLLFDKVHLIEDVLKIVEEISFEEVNQLAKEIIRPEEMTVSVVGKLNKKDKRWLENVNSA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 8098035 [H]