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The map label for this gene is Dut

Identifier: 20807831

GI number: 20807831

Start: 1367199

End: 1367645

Strand: Reverse

Name: Dut

Synonym: TTE1384

Alternate gene names: 20807831

Gene position: 1367645-1367199 (Counterclockwise)

Preceding gene: 20807832

Following gene: 20807830

Centisome position: 50.85

GC content: 40.04

Gene sequence:

>447_bases
ATGTCAATAGTGCTTAAGATAAAGAGAACAGAAGATGCAAAAGATTTACCTTTACCCGCTTATATGAGCGAAGGAGCTGC
GGGAATGGACTTATACGCCAATGTAAAGGGTGAGGTAACTATCAATCCAGGTGAAGTAGAACTCATACCTACAGGAATAC
AGATTGAACTTCCTCCGAATTATGAAGCGCAGATAAGGCCTAGAAGCGGTCTGGCCTTAAATTACGGTATAACCTTATTA
AACACTCCGGGAACTGTGGATTCTGATTACAGAGGAGAAATTAAACTGATTGTCATAAACCTCGGTAAACAGCCTGTCAC
AATTAAAAGAGGTCAAAGAATTGCTCAAATGGTGATAAACCAAGTAGTAAGGCCTAAAATAATAGAAGTAGAAGAACTTT
CAGAGACAGAGAGGATGGACAGAGGATTTGGCCATACAGGGGTATAA

Upstream 100 bases:

>100_bases
GAGGAAGTAAACCAGCTGGCAAAAGAGATAATAAGGCCAGAAGAAATGACTGTATCTGTCGTGGGTAAACTTAATAAAAA
AGATAAAAGGTGGTTGGAAA

Downstream 100 bases:

>100_bases
GGGGGGGAATGTCTGTGAGACTGAGTGAGTTTGGTAGTAAAGAGATTGTAAATATTGTAGACGGCAAGCGCTGGGGCTTG
GTGGAAGATTCCGATTTGAT

Product: dUTPase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MSIVLKIKRTEDAKDLPLPAYMSEGAAGMDLYANVKGEVTINPGEVELIPTGIQIELPPNYEAQIRPRSGLALNYGITLL
NTPGTVDSDYRGEIKLIVINLGKQPVTIKRGQRIAQMVINQVVRPKIIEVEELSETERMDRGFGHTGV

Sequences:

>Translated_148_residues
MSIVLKIKRTEDAKDLPLPAYMSEGAAGMDLYANVKGEVTINPGEVELIPTGIQIELPPNYEAQIRPRSGLALNYGITLL
NTPGTVDSDYRGEIKLIVINLGKQPVTIKRGQRIAQMVINQVVRPKIIEVEELSETERMDRGFGHTGV
>Mature_147_residues
SIVLKIKRTEDAKDLPLPAYMSEGAAGMDLYANVKGEVTINPGEVELIPTGIQIELPPNYEAQIRPRSGLALNYGITLLN
TPGTVDSDYRGEIKLIVINLGKQPVTIKRGQRIAQMVINQVVRPKIIEVEELSETERMDRGFGHTGV

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=129, Percent_Identity=41.0852713178295, Blast_Score=100, Evalue=8e-22,
Organism=Homo sapiens, GI4503423, Length=129, Percent_Identity=41.0852713178295, Blast_Score=99, Evalue=1e-21,
Organism=Homo sapiens, GI70906441, Length=129, Percent_Identity=41.0852713178295, Blast_Score=98, Evalue=3e-21,
Organism=Escherichia coli, GI1790071, Length=146, Percent_Identity=41.7808219178082, Blast_Score=118, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71988561, Length=146, Percent_Identity=39.7260273972603, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319729, Length=146, Percent_Identity=36.986301369863, Blast_Score=84, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24583610, Length=148, Percent_Identity=33.7837837837838, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19921126, Length=148, Percent_Identity=33.7837837837838, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_THETN (Q8RA46)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623002.1
- ProteinModelPortal:   Q8RA46
- SMR:   Q8RA46
- GeneID:   997857
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1384
- NMPDR:   fig|273068.3.peg.1349
- HOGENOM:   HBG436079
- OMA:   HGIALVN
- ProtClustDB:   CLSK901205
- BioCyc:   TTEN273068:TTE1384-MONOMER
- BRENDA:   3.6.1.23
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16297; Mature: 16166

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: BINDING 81-81 BINDING 95-95

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIVLKIKRTEDAKDLPLPAYMSEGAAGMDLYANVKGEVTINPGEVELIPTGIQIELPPN
CEEEEEEECCCCCCCCCCCHHHCCCCCCCEEEECCCCEEEECCCCEEEEECCEEEEECCC
YEAQIRPRSGLALNYGITLLNTPGTVDSDYRGEIKLIVINLGKQPVTIKRGQRIAQMVIN
CCEEEECCCCEEEECCEEEEECCCCCCCCCCCEEEEEEEECCCCCEEEHHHHHHHHHHHH
QVVRPKIIEVEELSETERMDRGFGHTGV
HHCCCEEEEHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SIVLKIKRTEDAKDLPLPAYMSEGAAGMDLYANVKGEVTINPGEVELIPTGIQIELPPN
EEEEEEECCCCCCCCCCCHHHCCCCCCCEEEECCCCEEEECCCCEEEEECCEEEEECCC
YEAQIRPRSGLALNYGITLLNTPGTVDSDYRGEIKLIVINLGKQPVTIKRGQRIAQMVIN
CCEEEECCCCEEEECCEEEEECCCCCCCCCCCEEEEEEEECCCCCEEEHHHHHHHHHHHH
QVVRPKIIEVEELSETERMDRGFGHTGV
HHCCCEEEEHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336