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The map label for this gene is LysC [H]

Identifier: 20807829

GI number: 20807829

Start: 1365669

End: 1366865

Strand: Reverse

Name: LysC [H]

Synonym: TTE1382

Alternate gene names: 20807829

Gene position: 1366865-1365669 (Counterclockwise)

Preceding gene: 20807830

Following gene: 20807828

Centisome position: 50.82

GC content: 38.68

Gene sequence:

>1197_bases
GTGAAAATATTGGTGCAAAAGTTTGGAGGAACATCTGTGTCTTCAGAAGAAAAAAGAGAGAAAGCTGTTTCAAAAGTAAT
AGAAGCAGTAAAAAATGGCTATAACGTGGTAGTAGTAGTGTCTGCTATGGGGAGAAACGGCGACCCTTATGCCACAGATA
CTTTAATAAACATGGCAAAATCAATTTACAGTGATATTCCAAAAAGAGAACTTGACTTAATAATGAACTGCGGTGAAATA
ATATCTAGTGTGCTTTTCGCTGCAACTCTGTCCAATAAAGGATATAAAGCAAAAGCTTTGACAGGAGGACAAGCTGGAAT
CATAACGGACGATAATTTTGGAAATGCAGAGGTCATAAATGTAGATCCTTCTAGAATTTTAGAAGTTTTAAGAGAAGGAA
TAATCCCTGTAGTAGCAGGCTTTCAAGGAATCACGCAAAAAGGAGAGCTCACTACATTGGGGCGCGGTGGAAGTGACACT
ACAGCAGCGCTTTTAGGAGTGGCTTTAAAAGCAGAAGCTGTAGAGATATACACTGATGTAGATGGGATAATGACAGCAGA
CCCAAGAATTGTATCAACTGCTCATATCATAGATAAAATAAGCTACAATGAAGTTTTTCAATTCGCTGAACAAGGGGCAA
AAGTAGTCCACCCGCGAGCTGTGGAAATAGCCATGAGGGGAAATATCCCTTTAATTATAAAAAATACTATGTCAGACAGC
GGCGGGACTATCATAACTAATTACAGTGATGCCTACGACAGCATATATGAAGTAGACAAATTGGTAACGGGCATTGCCAA
TATGAACAACAGGGTCCAGATTACAATTGAAAGTACAGAAAATGTCCAGGATATATTTGAGAAGATAGGAGAAGAAAAAA
TTAGCATCGACTTGATAAGCATATTCCCTGACAAACAGGTGTTTACTATATCTGAAGCTGATTTTGAAAAACTTAGAAGG
CTTTTTGAAAAATACGGGGTGAAATACTCCTACAGGACAAATTGCGCAAAAGTTTCTATCATAGGCAACAGAATAAGAGG
AGTGCCAGGTGTCATGGCTAGAGTCATCAAAGCATTATCTAAGCGCAATATTGAAATATATCAGACAGCAGACTCACACA
ATACCATCTCTTGTCTGGTAAGTCAGGATAAGGTAGAAGAGGCCATAAGAGTTCTCCACGACGAATTTAGCCTGTAA

Upstream 100 bases:

>100_bases
GAGTTCGAAGGGAAGCCAAGAAAATTTTATTAAAGTACTTTTCTTTGGTGCCGTAGTAAAGTATAATAGTAACTGATACT
AAAAAAGAAAGGATGAGACT

Downstream 100 bases:

>100_bases
ATATGCATACTCTCCTTCTTTTTTTTTTATACTAATATCTAGGAGAGTGATGCAGATGAAATTTGTAAACGAGGAAGGAA
CAGAACAACCACAAGCCCTT

Product: aspartate kinase I

Products: NA

Alternate protein names: Aspartate kinase 1; Aspartokinase I [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MKILVQKFGGTSVSSEEKREKAVSKVIEAVKNGYNVVVVVSAMGRNGDPYATDTLINMAKSIYSDIPKRELDLIMNCGEI
ISSVLFAATLSNKGYKAKALTGGQAGIITDDNFGNAEVINVDPSRILEVLREGIIPVVAGFQGITQKGELTTLGRGGSDT
TAALLGVALKAEAVEIYTDVDGIMTADPRIVSTAHIIDKISYNEVFQFAEQGAKVVHPRAVEIAMRGNIPLIIKNTMSDS
GGTIITNYSDAYDSIYEVDKLVTGIANMNNRVQITIESTENVQDIFEKIGEEKISIDLISIFPDKQVFTISEADFEKLRR
LFEKYGVKYSYRTNCAKVSIIGNRIRGVPGVMARVIKALSKRNIEIYQTADSHNTISCLVSQDKVEEAIRVLHDEFSL

Sequences:

>Translated_398_residues
MKILVQKFGGTSVSSEEKREKAVSKVIEAVKNGYNVVVVVSAMGRNGDPYATDTLINMAKSIYSDIPKRELDLIMNCGEI
ISSVLFAATLSNKGYKAKALTGGQAGIITDDNFGNAEVINVDPSRILEVLREGIIPVVAGFQGITQKGELTTLGRGGSDT
TAALLGVALKAEAVEIYTDVDGIMTADPRIVSTAHIIDKISYNEVFQFAEQGAKVVHPRAVEIAMRGNIPLIIKNTMSDS
GGTIITNYSDAYDSIYEVDKLVTGIANMNNRVQITIESTENVQDIFEKIGEEKISIDLISIFPDKQVFTISEADFEKLRR
LFEKYGVKYSYRTNCAKVSIIGNRIRGVPGVMARVIKALSKRNIEIYQTADSHNTISCLVSQDKVEEAIRVLHDEFSL
>Mature_398_residues
MKILVQKFGGTSVSSEEKREKAVSKVIEAVKNGYNVVVVVSAMGRNGDPYATDTLINMAKSIYSDIPKRELDLIMNCGEI
ISSVLFAATLSNKGYKAKALTGGQAGIITDDNFGNAEVINVDPSRILEVLREGIIPVVAGFQGITQKGELTTLGRGGSDT
TAALLGVALKAEAVEIYTDVDGIMTADPRIVSTAHIIDKISYNEVFQFAEQGAKVVHPRAVEIAMRGNIPLIIKNTMSDS
GGTIITNYSDAYDSIYEVDKLVTGIANMNNRVQITIESTENVQDIFEKIGEEKISIDLISIFPDKQVFTISEADFEKLRR
LFEKYGVKYSYRTNCAKVSIIGNRIRGVPGVMARVIKALSKRNIEIYQTADSHNTISCLVSQDKVEEAIRVLHDEFSL

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartokinase family [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=277, Percent_Identity=31.7689530685921, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1790455, Length=343, Percent_Identity=31.4868804664723, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1790376, Length=176, Percent_Identity=28.4090909090909, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320893, Length=376, Percent_Identity=28.1914893617021, Blast_Score=131, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 43501; Mature: 43501

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVQKFGGTSVSSEEKREKAVSKVIEAVKNGYNVVVVVSAMGRNGDPYATDTLINMAK
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHH
SIYSDIPKRELDLIMNCGEIISSVLFAATLSNKGYKAKALTGGQAGIITDDNFGNAEVIN
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEECCCCCCEEEEE
VDPSRILEVLREGIIPVVAGFQGITQKGELTTLGRGGSDTTAALLGVALKAEAVEIYTDV
CCHHHHHHHHHHCCHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCEEEEEECC
DGIMTADPRIVSTAHIIDKISYNEVFQFAEQGAKVVHPRAVEIAMRGNIPLIIKNTMSDS
CCCEECCCCHHHHHHHHHHCCHHHHHHHHHCCCEEECCCEEEEEEECCCCEEEEECCCCC
GGTIITNYSDAYDSIYEVDKLVTGIANMNNRVQITIESTENVQDIFEKIGEEKISIDLIS
CCEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHCCCEEEEEEEE
IFPDKQVFTISEADFEKLRRLFEKYGVKYSYRTNCAKVSIIGNRIRGVPGVMARVIKALS
ECCCCEEEEECHHHHHHHHHHHHHHCCEEEECCCCEEEEEECCHHCCCHHHHHHHHHHHH
KRNIEIYQTADSHNTISCLVSQDKVEEAIRVLHDEFSL
HCCEEEEEECCCCCEEEEEECHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKILVQKFGGTSVSSEEKREKAVSKVIEAVKNGYNVVVVVSAMGRNGDPYATDTLINMAK
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHH
SIYSDIPKRELDLIMNCGEIISSVLFAATLSNKGYKAKALTGGQAGIITDDNFGNAEVIN
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEECCCCCCEEEEE
VDPSRILEVLREGIIPVVAGFQGITQKGELTTLGRGGSDTTAALLGVALKAEAVEIYTDV
CCHHHHHHHHHHCCHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCEEEEEECC
DGIMTADPRIVSTAHIIDKISYNEVFQFAEQGAKVVHPRAVEIAMRGNIPLIIKNTMSDS
CCCEECCCCHHHHHHHHHHCCHHHHHHHHHCCCEEECCCEEEEEEECCCCEEEEECCCCC
GGTIITNYSDAYDSIYEVDKLVTGIANMNNRVQITIESTENVQDIFEKIGEEKISIDLIS
CCEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHCCCEEEEEEEE
IFPDKQVFTISEADFEKLRRLFEKYGVKYSYRTNCAKVSIIGNRIRGVPGVMARVIKALS
ECCCCEEEEECHHHHHHHHHHHHHHCCEEEECCCCEEEEEECCHHCCCHHHHHHHHHHHH
KRNIEIYQTADSHNTISCLVSQDKVEEAIRVLHDEFSL
HCCEEEEEECCCCCEEEEEECHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8098035; 9384377 [H]