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The map label for this gene is PurB [H]

Identifier: 20807814

GI number: 20807814

Start: 1349588

End: 1351006

Strand: Reverse

Name: PurB [H]

Synonym: TTE1367

Alternate gene names: 20807814

Gene position: 1351006-1349588 (Counterclockwise)

Preceding gene: 20807815

Following gene: 20807813

Centisome position: 50.23

GC content: 37.28

Gene sequence:

>1419_bases
ATGAGAGAGGTTTATGAAACACCTTTGGTCACAAGGTATGCGAGTAAAGAAATGGCACAAATTTTTTCTGATGATGAGAA
GTTTAAAACCTGGAGAAAATTGTGGGTGGCTTTAGCCAAAAGTGAAAAGGAATTAGGTCTTAATATAACTAATGAACAAA
TAGAAGAAATGGAAAAAAATATAGAAAATATAAATTATGAAGATGCGGAAAGGTTCGAAAAAGAATTCAGGCACGATGTA
ATGGCTCATATTCACGCCTACGGGCTTTTATGCCCAAAAGCAAAGCCTATAATTCACTTAGGCGCAACTTCTGCGTTTGT
AGATGACAATACGGATATTATTTTAATGGATAGAGGGCTTCAGCTAATTGAGAAAAAGCTTTTAAAAGCGATAAAAGTCC
TCTCTGAATTTGCACTAAAATATAAAGATTTACCAACATTGGGTTATACTCACTTTCAGCCTGCTCAGCTGACAACTGTA
GGTAAAAGAGCCTGTCTCTGGATACAGGACCTGATCCTTGACCTTCAGGATTTAAGATACAGGCGTCAAAACATGATGCT
GAGAGGAGTAAAAGGAACGACAGGTACTCAAGCTAGTTTTATGGAACTTTTTGATGGAGATGAGGAAAAGGTAAAAGAGC
TTGACAGGTTGGTTGTAGAAAAGATGGGGTATAAAAAATCCTTTCCTGTAACAGGTCAGACTTACACTCGAAAGTACGAT
TTTATGATTTTATCTGTGTTAAGTGGGATAGCTCAAAGTGCCCACAAGTTTAGCAACGACATAAGGCTTTTGCAACATTT
AAGGGAAATCGAAGAACCTTTTGAAGAAAAGCAAATTGGTTCTTCTGCTATGGCTTACAAAAGAAACCCTATGAGAAGTG
AAAGAATGGCAGCTTTATCTAGGTATGTAATTGTGACATTATTAAACTCTTCTATCACGGCCTCCAACCAGTGGTTTGAG
AGGACCTTGGATGATTCAGCTAACAGGAGAATTGTAATTCCCGAGATGTTTTTGGCCACGGATGCTATTTTAAACCTTTA
TATAAACATAGCTTCCGGGTTGAAAGTGAACGAAAAAGTGATTGAAAAAAACGTAAAAAGAGAACTTCCATTTATGGCAA
CAGAAAAAATTTTAATGGCGGCTGTGAAAAAGGGCGGAGATAGACAGGAATTGCACGAAGCCATAAGAGTATATTCCTTA
AAGGCTGCTGAAGAAGTAAAAAATGGCGAAGACAATAGACTGCTTGAGTATATCAAGCAGGATAAAAGATTTGGCCTTTC
TCATGAGGAAATTCTTGACCTTACTGATCCAAGAAAGTTTACAGGCAGATCTGCAAATCAGGTAAAGGAATTTATTGAAG
ACCATGTAAGACCTCTTCTTGATGGGATAGAAGACATAGAAGTCAAAGTAGAAGTTTAA

Upstream 100 bases:

>100_bases
GTAAAAGCTAAATAAAGCGAATAAAAACGTAAACAAAAGCCCCTGTTTTTACATCTTACAGGGGCTTTAATAAATATTTT
AAAAGCAGGAGGAAGGAAAT

Downstream 100 bases:

>100_bases
AAACTTTCACTTTCCACATCCCAATATCATAAAATAGGATTGAAAATGATATTGGGATGTGGTGTGATATGGGAATATGG
CCCGTATTTTTTGAAGGGTA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MREVYETPLVTRYASKEMAQIFSDDEKFKTWRKLWVALAKSEKELGLNITNEQIEEMEKNIENINYEDAERFEKEFRHDV
MAHIHAYGLLCPKAKPIIHLGATSAFVDDNTDIILMDRGLQLIEKKLLKAIKVLSEFALKYKDLPTLGYTHFQPAQLTTV
GKRACLWIQDLILDLQDLRYRRQNMMLRGVKGTTGTQASFMELFDGDEEKVKELDRLVVEKMGYKKSFPVTGQTYTRKYD
FMILSVLSGIAQSAHKFSNDIRLLQHLREIEEPFEEKQIGSSAMAYKRNPMRSERMAALSRYVIVTLLNSSITASNQWFE
RTLDDSANRRIVIPEMFLATDAILNLYINIASGLKVNEKVIEKNVKRELPFMATEKILMAAVKKGGDRQELHEAIRVYSL
KAAEEVKNGEDNRLLEYIKQDKRFGLSHEEILDLTDPRKFTGRSANQVKEFIEDHVRPLLDGIEDIEVKVEV

Sequences:

>Translated_472_residues
MREVYETPLVTRYASKEMAQIFSDDEKFKTWRKLWVALAKSEKELGLNITNEQIEEMEKNIENINYEDAERFEKEFRHDV
MAHIHAYGLLCPKAKPIIHLGATSAFVDDNTDIILMDRGLQLIEKKLLKAIKVLSEFALKYKDLPTLGYTHFQPAQLTTV
GKRACLWIQDLILDLQDLRYRRQNMMLRGVKGTTGTQASFMELFDGDEEKVKELDRLVVEKMGYKKSFPVTGQTYTRKYD
FMILSVLSGIAQSAHKFSNDIRLLQHLREIEEPFEEKQIGSSAMAYKRNPMRSERMAALSRYVIVTLLNSSITASNQWFE
RTLDDSANRRIVIPEMFLATDAILNLYINIASGLKVNEKVIEKNVKRELPFMATEKILMAAVKKGGDRQELHEAIRVYSL
KAAEEVKNGEDNRLLEYIKQDKRFGLSHEEILDLTDPRKFTGRSANQVKEFIEDHVRPLLDGIEDIEVKVEV
>Mature_472_residues
MREVYETPLVTRYASKEMAQIFSDDEKFKTWRKLWVALAKSEKELGLNITNEQIEEMEKNIENINYEDAERFEKEFRHDV
MAHIHAYGLLCPKAKPIIHLGATSAFVDDNTDIILMDRGLQLIEKKLLKAIKVLSEFALKYKDLPTLGYTHFQPAQLTTV
GKRACLWIQDLILDLQDLRYRRQNMMLRGVKGTTGTQASFMELFDGDEEKVKELDRLVVEKMGYKKSFPVTGQTYTRKYD
FMILSVLSGIAQSAHKFSNDIRLLQHLREIEEPFEEKQIGSSAMAYKRNPMRSERMAALSRYVIVTLLNSSITASNQWFE
RTLDDSANRRIVIPEMFLATDAILNLYINIASGLKVNEKVIEKNVKRELPFMATEKILMAAVKKGGDRQELHEAIRVYSL
KAAEEVKNGEDNRLLEYIKQDKRFGLSHEEILDLTDPRKFTGRSANQVKEFIEDHVRPLLDGIEDIEVKVEV

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=470, Percent_Identity=57.0212765957447, Blast_Score=562, Evalue=1e-160,
Organism=Homo sapiens, GI183227688, Length=385, Percent_Identity=59.7402597402597, Blast_Score=499, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17508577, Length=466, Percent_Identity=41.8454935622318, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32564234, Length=343, Percent_Identity=46.9387755102041, Blast_Score=317, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6323391, Length=476, Percent_Identity=53.5714285714286, Blast_Score=500, Evalue=1e-142,
Organism=Drosophila melanogaster, GI24647570, Length=459, Percent_Identity=54.9019607843137, Blast_Score=514, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 54595; Mature: 54595

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREVYETPLVTRYASKEMAQIFSDDEKFKTWRKLWVALAKSEKELGLNITNEQIEEMEKN
CCCCHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHH
IENINYEDAERFEKEFRHDVMAHIHAYGLLCPKAKPIIHLGATSAFVDDNTDIILMDRGL
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEECCCCCEEEECCCH
QLIEKKLLKAIKVLSEFALKYKDLPTLGYTHFQPAQLTTVGKRACLWIQDLILDLQDLRY
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RRQNMMLRGVKGTTGTQASFMELFDGDEEKVKELDRLVVEKMGYKKSFPVTGQTYTRKYD
HHHHHHEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
FMILSVLSGIAQSAHKFSNDIRLLQHLREIEEPFEEKQIGSSAMAYKRNPMRSERMAALS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHH
RYVIVTLLNSSITASNQWFERTLDDSANRRIVIPEMFLATDAILNLYINIASGLKVNEKV
HHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHCCCCHHHHH
IEKNVKRELPFMATEKILMAAVKKGGDRQELHEAIRVYSLKAAEEVKNGEDNRLLEYIKQ
HHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
DKRFGLSHEEILDLTDPRKFTGRSANQVKEFIEDHVRPLLDGIEDIEVKVEV
HHHCCCCHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEC
>Mature Secondary Structure
MREVYETPLVTRYASKEMAQIFSDDEKFKTWRKLWVALAKSEKELGLNITNEQIEEMEKN
CCCCHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHH
IENINYEDAERFEKEFRHDVMAHIHAYGLLCPKAKPIIHLGATSAFVDDNTDIILMDRGL
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEECCCCCEEEECCCH
QLIEKKLLKAIKVLSEFALKYKDLPTLGYTHFQPAQLTTVGKRACLWIQDLILDLQDLRY
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RRQNMMLRGVKGTTGTQASFMELFDGDEEKVKELDRLVVEKMGYKKSFPVTGQTYTRKYD
HHHHHHEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
FMILSVLSGIAQSAHKFSNDIRLLQHLREIEEPFEEKQIGSSAMAYKRNPMRSERMAALS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHH
RYVIVTLLNSSITASNQWFERTLDDSANRRIVIPEMFLATDAILNLYINIASGLKVNEKV
HHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHCCCCHHHHH
IEKNVKRELPFMATEKILMAAVKKGGDRQELHEAIRVYSLKAAEEVKNGEDNRLLEYIKQ
HHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
DKRFGLSHEEILDLTDPRKFTGRSANQVKEFIEDHVRPLLDGIEDIEVKVEV
HHHCCCCHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]