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The map label for this gene is FliA2 [H]

Identifier: 20807766

GI number: 20807766

Start: 1306705

End: 1307427

Strand: Direct

Name: FliA2 [H]

Synonym: TTE1317

Alternate gene names: 20807766

Gene position: 1306705-1307427 (Clockwise)

Preceding gene: 20807765

Following gene: 20807767

Centisome position: 48.59

GC content: 36.65

Gene sequence:

>723_bases
ATGAAGGAGGAGGAAAATCTTGAGCTTATAAAGCTTGCAAAAAATAATGACAAAGCTAGTCTTGAAAAATTGATTTTGGA
AAACAGCGGCCTTATCTGGAGCATTGTAAAAAAATTTGCTAACAGAGGCTATGAATTAGAAGATTTATATCAAATAGGGT
GTATAGGATTTGTAAAGGCTGTGCAGAAGTTTGACGAATCTTACAATGTGAAGTTTTCTACATATGCTGTTCCCATAATA
ATGGGGGAGATAAAGAGGTTTTTAAGAGATGATGGATTGATAAAAGTCAGTAGGTCTTTGAAAGAACTTTCTTCTAAAGC
CTTTTACATGAAAGAAGTGTTGAGCAATGAGCTGAATAGAGAGCCTACTATTAACGAAATTGCAGAAAGGCTCAATGTAA
CCCCGGAAGAAATTGCTATGGCTTTTGAATCGGCTGCCACAGCGGAATCTCTTTATGACAGCATAAGCCATGAAGAAGAG
GACAGGCAGCTTATTGAAATGGTGGGAGATAAAGAAGATAGTGTAGATTTAGAAGAAAAGCTTACACTTCACATGATCAT
AGACAAGCTAAAACCTAGGGAGAAGCAAGTGATATTTTTGAGGTATTTTAAAGACATGACTCAGACGGAGGTAGCGAACA
TCCTGGGAATTTCTCAGGTGCAGGTTTCAAGGATAGAGAAAAAAGTTTTGGAAAAGATAAGGAAAGAATTAGAAAAAGTG
TAG

Upstream 100 bases:

>100_bases
GACCTTTATGGATGAGCTAGAAGTAGAGTCTACTCCAGGCAAAGGAACTTTAGTGAGAATGACTAAATACATAGGGCGAA
ATAGATGAGGTGACCATCCC

Downstream 100 bases:

>100_bases
CATTTGGCATGAATGCTACACTTTTTTTGAATTAATTCAACTTGTTCTTCACATACTAATACTGGCTGGTGAAATGAGAT
GAAAAAAATTTTAATTGCAA

Product: DNA-directed RNA polymerase specialized sigma subunit

Products: NA

Alternate protein names: Sporulation sigma factor; Stage II sporulation protein AC [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MKEEENLELIKLAKNNDKASLEKLILENSGLIWSIVKKFANRGYELEDLYQIGCIGFVKAVQKFDESYNVKFSTYAVPII
MGEIKRFLRDDGLIKVSRSLKELSSKAFYMKEVLSNELNREPTINEIAERLNVTPEEIAMAFESAATAESLYDSISHEEE
DRQLIEMVGDKEDSVDLEEKLTLHMIIDKLKPREKQVIFLRYFKDMTQTEVANILGISQVQVSRIEKKVLEKIRKELEKV

Sequences:

>Translated_240_residues
MKEEENLELIKLAKNNDKASLEKLILENSGLIWSIVKKFANRGYELEDLYQIGCIGFVKAVQKFDESYNVKFSTYAVPII
MGEIKRFLRDDGLIKVSRSLKELSSKAFYMKEVLSNELNREPTINEIAERLNVTPEEIAMAFESAATAESLYDSISHEEE
DRQLIEMVGDKEDSVDLEEKLTLHMIIDKLKPREKQVIFLRYFKDMTQTEVANILGISQVQVSRIEKKVLEKIRKELEKV
>Mature_240_residues
MKEEENLELIKLAKNNDKASLEKLILENSGLIWSIVKKFANRGYELEDLYQIGCIGFVKAVQKFDESYNVKFSTYAVPII
MGEIKRFLRDDGLIKVSRSLKELSSKAFYMKEVLSNELNREPTINEIAERLNVTPEEIAMAFESAATAESLYDSISHEEE
DRQLIEMVGDKEDSVDLEEKLTLHMIIDKLKPREKQVIFLRYFKDMTQTEVANILGISQVQVSRIEKKVLEKIRKELEKV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes. It is responsible for directing gene

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=218, Percent_Identity=31.651376146789, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1789098, Length=255, Percent_Identity=25.4901960784314, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788231, Length=207, Percent_Identity=29.951690821256, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014236
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27786; Mature: 27786

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEEENLELIKLAKNNDKASLEKLILENSGLIWSIVKKFANRGYELEDLYQIGCIGFVKA
CCCCCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VQKFDESYNVKFSTYAVPIIMGEIKRFLRDDGLIKVSRSLKELSSKAFYMKEVLSNELNR
HHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EPTINEIAERLNVTPEEIAMAFESAATAESLYDSISHEEEDRQLIEMVGDKEDSVDLEEK
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHH
LTLHMIIDKLKPREKQVIFLRYFKDMTQTEVANILGISQVQVSRIEKKVLEKIRKELEKV
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKEEENLELIKLAKNNDKASLEKLILENSGLIWSIVKKFANRGYELEDLYQIGCIGFVKA
CCCCCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VQKFDESYNVKFSTYAVPIIMGEIKRFLRDDGLIKVSRSLKELSSKAFYMKEVLSNELNR
HHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EPTINEIAERLNVTPEEIAMAFESAATAESLYDSISHEEEDRQLIEMVGDKEDSVDLEEK
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHH
LTLHMIIDKLKPREKQVIFLRYFKDMTQTEVANILGISQVQVSRIEKKVLEKIRKELEKV
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1391049 [H]