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The map label for this gene is proA

Identifier: 20807727

GI number: 20807727

Start: 1274003

End: 1275247

Strand: Direct

Name: proA

Synonym: TTE1278

Alternate gene names: 20807727

Gene position: 1274003-1275247 (Clockwise)

Preceding gene: 20807726

Following gene: 20807728

Centisome position: 47.37

GC content: 41.04

Gene sequence:

>1245_bases
ATGGAGGTTGAAGTAAAGGCTAAAAAGGCGAAAGAAGCGTCAAGAAAAATGGCTGTACTTGACACAGAAACTAAAAATAG
AGCTCTTATAAATATGGCAGAAGCTCTTTTGGAAAATGCAGATAAAATCTTAAAGGCCAATGAAAAAGACGTTTTAGAGG
CAGAAAGGAGAAATTTAAAAGCCTCTCTTGTGGACAGGCTAAAATTGGATGAAAAAAGGATAAAAGCTATGGCAGAAGGC
TTAAAAGAAGTGGCTTCTTTGAAAGACCCTGTCGGAGATATAGAGGAGATGTGGATAAGGCCCAATGGACTTCAAATAGG
AAAGATGCGCGTCCCCATAGGCGTCATAGGCATGATATACGAATCCCGCCCCAATGTCACTGCTGATGCTGCAGGCCTTT
GCCTTAAAGCAGGAAATGCCGTGATTTTAAGAGGAGGAAGTGATGCTATAAATTCTAACATCGCTATTGCTTCTATTCTG
GCAGAAGCTGCTTACAAAAGTGGTATTCCGGAAGGAGCGATACAGCTTATTGAAAACACTGACAGGGAAGAAGTAAATAG
GATGATGAAGTTAAACGGCTTGATTGACCTTATCATCCCAAGAGGTGGGACAAGCCTTATCAAAAACGTCATTGAAAATT
CTACTGTACCTGTTATAGAGACGGGAGTCGGGAATTGCCACATTTTTGTGGATGAGAGTGCAAATTTTGAAATGGCAAAG
GACATAATTGTAAATGCGAAGGTTCAAAGGCCTGGGGTGTGCAATGCAGTAGAGACTGTTTTGGTGCATAAGGGTATAGC
AGAAAGATTCCTGCCTGTTATGGTAAAAGAACTGTCATCTCATGGGGTAGAAATAAGAGGCTGTGAGCTTACAAAAAGGA
TATGCCCTGATGTAAAAGAAGCCACAGAAGAGGACTGGGCGACAGAGTACCTTGACCTCATATTAGCGGTAAAAGTTGTA
GAGAATATAGATGAGGCATTAGAGCACATTTCTAAGTATTCTACAGGTCACTCTGAGAGCATTGTAACTGAAAATTACAC
TAATGCCATGAGATTTTTAAAATCTGTAGATTCTGCTGCAGTATATGTGAACGCTTCTACTAGATTCACTGATGGAGGAG
AATTTGGATTTGGGGCGGAAATAGGCATAAGCACTCAAAAAATGCACGCAAGAGGGCCTATGGGGCTTAAAGAGCTTACG
ACCTACAAATATGTGATTTTGGGCAGTGGACAAATAAGGAAGTAA

Upstream 100 bases:

>100_bases
GGCTGTAAGACCCATGACATAGAGTCAATATTGGGGTATAAGTACTACGACGAGGTTATTCACAGGGACAATCTCGTAGT
TCTTGAGAGAGGTGAGAAGT

Downstream 100 bases:

>100_bases
GGTGTATTATAGTAACGTGAGGTGATATTATGAAAAGGGTGCTTGTAATTCACGGCCCCAATGTGAATCTTACTGGAAAG
AGGGAAAAAGAAGTCTATGG

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MEVEVKAKKAKEASRKMAVLDTETKNRALINMAEALLENADKILKANEKDVLEAERRNLKASLVDRLKLDEKRIKAMAEG
LKEVASLKDPVGDIEEMWIRPNGLQIGKMRVPIGVIGMIYESRPNVTADAAGLCLKAGNAVILRGGSDAINSNIAIASIL
AEAAYKSGIPEGAIQLIENTDREEVNRMMKLNGLIDLIIPRGGTSLIKNVIENSTVPVIETGVGNCHIFVDESANFEMAK
DIIVNAKVQRPGVCNAVETVLVHKGIAERFLPVMVKELSSHGVEIRGCELTKRICPDVKEATEEDWATEYLDLILAVKVV
ENIDEALEHISKYSTGHSESIVTENYTNAMRFLKSVDSAAVYVNASTRFTDGGEFGFGAEIGISTQKMHARGPMGLKELT
TYKYVILGSGQIRK

Sequences:

>Translated_414_residues
MEVEVKAKKAKEASRKMAVLDTETKNRALINMAEALLENADKILKANEKDVLEAERRNLKASLVDRLKLDEKRIKAMAEG
LKEVASLKDPVGDIEEMWIRPNGLQIGKMRVPIGVIGMIYESRPNVTADAAGLCLKAGNAVILRGGSDAINSNIAIASIL
AEAAYKSGIPEGAIQLIENTDREEVNRMMKLNGLIDLIIPRGGTSLIKNVIENSTVPVIETGVGNCHIFVDESANFEMAK
DIIVNAKVQRPGVCNAVETVLVHKGIAERFLPVMVKELSSHGVEIRGCELTKRICPDVKEATEEDWATEYLDLILAVKVV
ENIDEALEHISKYSTGHSESIVTENYTNAMRFLKSVDSAAVYVNASTRFTDGGEFGFGAEIGISTQKMHARGPMGLKELT
TYKYVILGSGQIRK
>Mature_414_residues
MEVEVKAKKAKEASRKMAVLDTETKNRALINMAEALLENADKILKANEKDVLEAERRNLKASLVDRLKLDEKRIKAMAEG
LKEVASLKDPVGDIEEMWIRPNGLQIGKMRVPIGVIGMIYESRPNVTADAAGLCLKAGNAVILRGGSDAINSNIAIASIL
AEAAYKSGIPEGAIQLIENTDREEVNRMMKLNGLIDLIIPRGGTSLIKNVIENSTVPVIETGVGNCHIFVDESANFEMAK
DIIVNAKVQRPGVCNAVETVLVHKGIAERFLPVMVKELSSHGVEIRGCELTKRICPDVKEATEEDWATEYLDLILAVKVV
ENIDEALEHISKYSTGHSESIVTENYTNAMRFLKSVDSAAVYVNASTRFTDGGEFGFGAEIGISTQKMHARGPMGLKELT
TYKYVILGSGQIRK

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI62912457, Length=414, Percent_Identity=37.43961352657, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI21361368, Length=414, Percent_Identity=37.43961352657, Blast_Score=270, Evalue=2e-72,
Organism=Escherichia coli, GI1786438, Length=408, Percent_Identity=47.3039215686275, Blast_Score=391, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17569967, Length=415, Percent_Identity=35.9036144578313, Blast_Score=261, Evalue=6e-70,
Organism=Caenorhabditis elegans, GI17569969, Length=415, Percent_Identity=35.9036144578313, Blast_Score=261, Evalue=7e-70,
Organism=Saccharomyces cerevisiae, GI6324899, Length=427, Percent_Identity=37.7049180327869, Blast_Score=288, Evalue=2e-78,
Organism=Drosophila melanogaster, GI21357643, Length=413, Percent_Identity=37.046004842615, Blast_Score=258, Evalue=6e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_THETN (Q8RAE5)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_622898.1
- ProteinModelPortal:   Q8RAE5
- SMR:   Q8RAE5
- GeneID:   997604
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1278
- NMPDR:   fig|273068.3.peg.1116
- HOGENOM:   HBG318080
- OMA:   QYPAACN
- ProtClustDB:   PRK00197
- BioCyc:   TTEN273068:TTE1278-MONOMER
- BRENDA:   1.2.1.41
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PANTHER:   PTHR11063:SF1
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 45249; Mature: 45249

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVEVKAKKAKEASRKMAVLDTETKNRALINMAEALLENADKILKANEKDVLEAERRNLK
CCCEEHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
ASLVDRLKLDEKRIKAMAEGLKEVASLKDPVGDIEEMWIRPNGLQIGKMRVPIGVIGMIY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEEECCHHHEEHEE
ESRPNVTADAAGLCLKAGNAVILRGGSDAINSNIAIASILAEAAYKSGIPEGAIQLIENT
ECCCCCCHHHHHHEEECCCEEEEECCCHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCC
DREEVNRMMKLNGLIDLIIPRGGTSLIKNVIENSTVPVIETGVGNCHIFVDESANFEMAK
CHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCCEEEECCCEEEEEEECCCCHHHHH
DIIVNAKVQRPGVCNAVETVLVHKGIAERFLPVMVKELSSHGVEIRGCELTKRICPDVKE
HHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHCCCHHH
ATEEDWATEYLDLILAVKVVENIDEALEHISKYSTGHSESIVTENYTNAMRFLKSVDSAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEE
VYVNASTRFTDGGEFGFGAEIGISTQKMHARGPMGLKELTTYKYVILGSGQIRK
EEEECCCEECCCCCCCCCCEECCCHHHHHCCCCCCHHHHHHEEEEEECCCCCCC
>Mature Secondary Structure
MEVEVKAKKAKEASRKMAVLDTETKNRALINMAEALLENADKILKANEKDVLEAERRNLK
CCCEEHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
ASLVDRLKLDEKRIKAMAEGLKEVASLKDPVGDIEEMWIRPNGLQIGKMRVPIGVIGMIY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEEECCHHHEEHEE
ESRPNVTADAAGLCLKAGNAVILRGGSDAINSNIAIASILAEAAYKSGIPEGAIQLIENT
ECCCCCCHHHHHHEEECCCEEEEECCCHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCC
DREEVNRMMKLNGLIDLIIPRGGTSLIKNVIENSTVPVIETGVGNCHIFVDESANFEMAK
CHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCCEEEECCCEEEEEEECCCCHHHHH
DIIVNAKVQRPGVCNAVETVLVHKGIAERFLPVMVKELSSHGVEIRGCELTKRICPDVKE
HHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHCCCHHH
ATEEDWATEYLDLILAVKVVENIDEALEHISKYSTGHSESIVTENYTNAMRFLKSVDSAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEE
VYVNASTRFTDGGEFGFGAEIGISTQKMHARGPMGLKELTTYKYVILGSGQIRK
EEEECCCEECCCCCCCCCCEECCCHHHHHCCCCCCHHHHHHEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336