Click here to switch to the map view.

The map label for this gene is PppA [H]

Identifier: 20807715

GI number: 20807715

Start: 1266112

End: 1266870

Strand: Direct

Name: PppA [H]

Synonym: TTE1266

Alternate gene names: 20807715

Gene position: 1266112-1266870 (Clockwise)

Preceding gene: 20807714

Following gene: 20807716

Centisome position: 47.08

GC content: 33.86

Gene sequence:

>759_bases
ATGAAAATGCTAATTTACATACTCATATTTCTATTCGGCACAATTATAGGAAGTTTTTTAAATGTAGTCATATACAGAGT
TCCCAGAAAAGAATCCATAATATACCCTTCCTCCCATTGTCCCAGATGTGGTCATGAATTAAATGGTATTGATTTAATAC
CAATATTGAGTTATATTTTACTTAAAGGAAGATGCAGGTACTGTGGAGAAAAAATTTCAATAAGATACCCTGTTGTGGAG
CTGCTTACAGGAATTGTATTTGTAATAATTTACTCTAAATTTGGGTTGGACGTGAAGGCTTTTTCTTACATGTTTCTTTC
TTCTGTGCTGATTTCAGCAAGTTTTATAGATTTGGAACACAAAATTATACCCAATAGCATAATAATAGCTGGATTTATAG
GTGCTTTAATTTTTAGACTACTCATGTATAGTTATGGCTTTTTAGACTACATTCTGGGATTTTTATTGGGTGGGGGAATA
CTCCTTCTCATCTCTCTTCTCTCAGGAGGGGAAATGGGGGGAGGAGATGTAAAATTGATGGCTCTTATAGGCTTTTTTAT
AGGATGGAAGCTTGTGCTTTTAAATTTGTTGCTGGGTGTTGTGTTGGGAGCTTTGGCAGGTGTTTTACTTGTACTTTTTA
AGATAAAGAGCAGAAAGGACTACATACCTTTTGCACCTTATCTATCTTTAGGATGGCTTATTTCAATCTTATACGGATAT
GAGATTTTAAATTACTATTTAAAACTCATAAGGGGCTAA

Upstream 100 bases:

>100_bases
GATTTTAAGTACGATCCTTCTACTGGCAAAGTTACTTTACCATAACTTCAGGGCACGTTTTTGTGCCCTCCTTACTATTT
TTTAGGAGGCAATTTTAATC

Downstream 100 bases:

>100_bases
GCTTATGAAGGGGGAAGAGGGGCTAACTTTAATAGAGCTCATATCAACTTTAGCTATTTTGTCTTTAATTTTTCTACTAG
TTATCCCTTCTACCAGTTTT

Product: Signal peptidase, cleaves prepilin-like proteins

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKMLIYILIFLFGTIIGSFLNVVIYRVPRKESIIYPSSHCPRCGHELNGIDLIPILSYILLKGRCRYCGEKISIRYPVVE
LLTGIVFVIIYSKFGLDVKAFSYMFLSSVLISASFIDLEHKIIPNSIIIAGFIGALIFRLLMYSYGFLDYILGFLLGGGI
LLLISLLSGGEMGGGDVKLMALIGFFIGWKLVLLNLLLGVVLGALAGVLLVLFKIKSRKDYIPFAPYLSLGWLISILYGY
EILNYYLKLIRG

Sequences:

>Translated_252_residues
MKMLIYILIFLFGTIIGSFLNVVIYRVPRKESIIYPSSHCPRCGHELNGIDLIPILSYILLKGRCRYCGEKISIRYPVVE
LLTGIVFVIIYSKFGLDVKAFSYMFLSSVLISASFIDLEHKIIPNSIIIAGFIGALIFRLLMYSYGFLDYILGFLLGGGI
LLLISLLSGGEMGGGDVKLMALIGFFIGWKLVLLNLLLGVVLGALAGVLLVLFKIKSRKDYIPFAPYLSLGWLISILYGY
EILNYYLKLIRG
>Mature_252_residues
MKMLIYILIFLFGTIIGSFLNVVIYRVPRKESIIYPSSHCPRCGHELNGIDLIPILSYILLKGRCRYCGEKISIRYPVVE
LLTGIVFVIIYSKFGLDVKAFSYMFLSSVLISASFIDLEHKIIPNSIIIAGFIGALIFRLLMYSYGFLDYILGFLLGGGI
LLLISLLSGGEMGGGDVKLMALIGFFIGWKLVLLNLLLGVVLGALAGVLLVLFKIKSRKDYIPFAPYLSLGWLISILYGY
EILNYYLKLIRG

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=252, Percent_Identity=34.1269841269841, Blast_Score=107, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 28160; Mature: 28160

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMLIYILIFLFGTIIGSFLNVVIYRVPRKESIIYPSSHCPRCGHELNGIDLIPILSYIL
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCEEHHHHHHHHH
LKGRCRYCGEKISIRYPVVELLTGIVFVIIYSKFGLDVKAFSYMFLSSVLISASFIDLEH
HHCHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
KIIPNSIIIAGFIGALIFRLLMYSYGFLDYILGFLLGGGILLLISLLSGGEMGGGDVKLM
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
ALIGFFIGWKLVLLNLLLGVVLGALAGVLLVLFKIKSRKDYIPFAPYLSLGWLISILYGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
EILNYYLKLIRG
HHHHHHHHHHCC
>Mature Secondary Structure
MKMLIYILIFLFGTIIGSFLNVVIYRVPRKESIIYPSSHCPRCGHELNGIDLIPILSYIL
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCEEHHHHHHHHH
LKGRCRYCGEKISIRYPVVELLTGIVFVIIYSKFGLDVKAFSYMFLSSVLISASFIDLEH
HHCHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
KIIPNSIIIAGFIGALIFRLLMYSYGFLDYILGFLLGGGILLLISLLSGGEMGGGDVKLM
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
ALIGFFIGWKLVLLNLLLGVVLGALAGVLLVLFKIKSRKDYIPFAPYLSLGWLISILYGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
EILNYYLKLIRG
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]