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The map label for this gene is PycA [H]

Identifier: 20807663

GI number: 20807663

Start: 1208420

End: 1209817

Strand: Direct

Name: PycA [H]

Synonym: TTE1208

Alternate gene names: 20807663

Gene position: 1208420-1209817 (Clockwise)

Preceding gene: 20807662

Following gene: 20807664

Centisome position: 44.93

GC content: 41.06

Gene sequence:

>1398_bases
ATGGAAAGGAGAGTTAAAATAACAGAAACAGTTCTAAGAGATGCCCATCAGTCTTTGATGGCTACTCGCATGACTACAGA
GGAAATGCTGCCTATTGCAGAAAAGCTTGATAAGGTGGGATATCATTCTCTTGAGGTTTGGGGAGGAGCTACCTTTGATG
CTTGTCTCAGGTTTTTAAATGAAGACCCGTGGGAGAGGCTGCGGGAATTAAAAAAGAGGATAAAAAATACACCCCTGCAA
ATGCTTTTAAGAGGGCAAAATCTGGTAGGATACAGACATTATCCGGATGACATTGTAGAAAAATTTATAGAAAAGGCTGT
AGCTAATGGAATAGACATAATAAGGATCTTTGATGCTTTGAATGATGTGAGAAATTTGGAAGTTTCCATAAAGGCGACAA
AAAGGGTAGGAGCCCATGCGCAAGGGACTCTTGTCTATACTATAAGCCCTGTCCACACTATAGACCACTACATAAGAGTG
GCAAAAGAACTGGTAGAATTAGGTGTAGATTCTATCTGCATAAAGGACATGTCGGGCATATTGAGCCCTTATGTAGCCTA
TGAACTGGTGAAGAGGCTCAAAGAGACAGTTAATGTTCCCATACAGCTGCACGGCCATTACACGAGTGGAATGGCTTCCA
TGACTTATTTAAAAGCCATAGAAGCAGGGGTGGATGCGATAGATACAGCAATTTCTCCTTTAGCTTTGGGCACTTCTCAG
CCTGCTACAGAGACCATGGTGGCAGCTTTGAAGGGAACTCAATATGATACAGGGCTTGACCTTGAACTTCTCTCAGAAAT
TGCTTCATATTTTAAGGAAGTAAAGCGAAATCACTATAAAGAAACAGATTTCTCAATGGTAATGGGTATAGATACTGATG
TGCTTGTCTACCAGGTTCCGGGAGGAATGCTTTCAAATCTCATTGCACAGCTAAAAGAGCAAAAAGCGCTTGACAAGTAT
AAAGAAGTTCTAGAAGAGATACCGAGGGTGAGAGAGGACCTAGGATATCCGCCTCTAGTTACTCCAATGAGTCAAATGGT
AGGTACACAGGCGGTTTTAAATGTGATAACTGGTGAGAGGTATAAAATGGTTCCAAAAGAGATAAAGGATTATGTCAAGG
GACTGTACGGCAGGCCTCCTGCACCAATTTCTGATGAGATAAAGAGGAAAATAATAGGTGATGAAGAGGTAATTGAGATA
AGGCCGGCGGACCTCTTAAAACCACAGTTTGAAGAGGTAAAAGAAGAAATAAAAGAGTATTACGAACAGGAAGAGGATGT
ACTGACATATGCTCTTTTTCCGCAAGTGGCTAAAAAGTTTTTTGAGTACAGAAGGGCGAAAAAGTATTTTATAGATTCTA
GTATGGTTGGTGAGGGAAACAGAGTGTATCCTGTCTAA

Upstream 100 bases:

>100_bases
TTTTTCTAGAATTAGCAGAAGGGTGCCTAGAGTGTATTTTTATGATGGAATAAAGATTGGAGAAGTAAATTACTTAAAGT
GAGGTGAAGGACTGTAAACC

Downstream 100 bases:

>100_bases
TTTAAATTCCAGAGGTGAAGGAAAATGTCGAAGGTGTTAATGCAGGGAAATGAAGCAGTGGTAGAAGGAGCAATAAGAGC
CGGGATGAGGTTTTACGCAG

Product: oxaloacetate decarboxylase

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MERRVKITETVLRDAHQSLMATRMTTEEMLPIAEKLDKVGYHSLEVWGGATFDACLRFLNEDPWERLRELKKRIKNTPLQ
MLLRGQNLVGYRHYPDDIVEKFIEKAVANGIDIIRIFDALNDVRNLEVSIKATKRVGAHAQGTLVYTISPVHTIDHYIRV
AKELVELGVDSICIKDMSGILSPYVAYELVKRLKETVNVPIQLHGHYTSGMASMTYLKAIEAGVDAIDTAISPLALGTSQ
PATETMVAALKGTQYDTGLDLELLSEIASYFKEVKRNHYKETDFSMVMGIDTDVLVYQVPGGMLSNLIAQLKEQKALDKY
KEVLEEIPRVREDLGYPPLVTPMSQMVGTQAVLNVITGERYKMVPKEIKDYVKGLYGRPPAPISDEIKRKIIGDEEVIEI
RPADLLKPQFEEVKEEIKEYYEQEEDVLTYALFPQVAKKFFEYRRAKKYFIDSSMVGEGNRVYPV

Sequences:

>Translated_465_residues
MERRVKITETVLRDAHQSLMATRMTTEEMLPIAEKLDKVGYHSLEVWGGATFDACLRFLNEDPWERLRELKKRIKNTPLQ
MLLRGQNLVGYRHYPDDIVEKFIEKAVANGIDIIRIFDALNDVRNLEVSIKATKRVGAHAQGTLVYTISPVHTIDHYIRV
AKELVELGVDSICIKDMSGILSPYVAYELVKRLKETVNVPIQLHGHYTSGMASMTYLKAIEAGVDAIDTAISPLALGTSQ
PATETMVAALKGTQYDTGLDLELLSEIASYFKEVKRNHYKETDFSMVMGIDTDVLVYQVPGGMLSNLIAQLKEQKALDKY
KEVLEEIPRVREDLGYPPLVTPMSQMVGTQAVLNVITGERYKMVPKEIKDYVKGLYGRPPAPISDEIKRKIIGDEEVIEI
RPADLLKPQFEEVKEEIKEYYEQEEDVLTYALFPQVAKKFFEYRRAKKYFIDSSMVGEGNRVYPV
>Mature_465_residues
MERRVKITETVLRDAHQSLMATRMTTEEMLPIAEKLDKVGYHSLEVWGGATFDACLRFLNEDPWERLRELKKRIKNTPLQ
MLLRGQNLVGYRHYPDDIVEKFIEKAVANGIDIIRIFDALNDVRNLEVSIKATKRVGAHAQGTLVYTISPVHTIDHYIRV
AKELVELGVDSICIKDMSGILSPYVAYELVKRLKETVNVPIQLHGHYTSGMASMTYLKAIEAGVDAIDTAISPLALGTSQ
PATETMVAALKGTQYDTGLDLELLSEIASYFKEVKRNHYKETDFSMVMGIDTDVLVYQVPGGMLSNLIAQLKEQKALDKY
KEVLEEIPRVREDLGYPPLVTPMSQMVGTQAVLNVITGERYKMVPKEIKDYVKGLYGRPPAPISDEIKRKIIGDEEVIEI
RPADLLKPQFEEVKEEIKEYYEQEEDVLTYALFPQVAKKFFEYRRAKKYFIDSSMVGEGNRVYPV

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=462, Percent_Identity=34.6320346320346, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI106049295, Length=462, Percent_Identity=34.6320346320346, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI106049292, Length=462, Percent_Identity=34.6320346320346, Blast_Score=263, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17562816, Length=462, Percent_Identity=36.3636363636364, Blast_Score=283, Evalue=1e-76,
Organism=Saccharomyces cerevisiae, GI6319695, Length=486, Percent_Identity=33.1275720164609, Blast_Score=271, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6321376, Length=465, Percent_Identity=33.9784946236559, Blast_Score=270, Evalue=3e-73,
Organism=Drosophila melanogaster, GI281363050, Length=462, Percent_Identity=37.6623376623377, Blast_Score=287, Evalue=8e-78,
Organism=Drosophila melanogaster, GI24652224, Length=462, Percent_Identity=37.6623376623377, Blast_Score=287, Evalue=8e-78,
Organism=Drosophila melanogaster, GI24652222, Length=462, Percent_Identity=37.6623376623377, Blast_Score=287, Evalue=8e-78,
Organism=Drosophila melanogaster, GI24652220, Length=462, Percent_Identity=37.6623376623377, Blast_Score=287, Evalue=8e-78,
Organism=Drosophila melanogaster, GI24652218, Length=462, Percent_Identity=37.6623376623377, Blast_Score=287, Evalue=8e-78,
Organism=Drosophila melanogaster, GI24652212, Length=462, Percent_Identity=37.6623376623377, Blast_Score=287, Evalue=9e-78,
Organism=Drosophila melanogaster, GI24652210, Length=462, Percent_Identity=37.6623376623377, Blast_Score=287, Evalue=9e-78,
Organism=Drosophila melanogaster, GI24652214, Length=462, Percent_Identity=37.6623376623377, Blast_Score=287, Evalue=9e-78,
Organism=Drosophila melanogaster, GI19921944, Length=462, Percent_Identity=37.6623376623377, Blast_Score=287, Evalue=9e-78,
Organism=Drosophila melanogaster, GI24652216, Length=462, Percent_Identity=37.6623376623377, Blast_Score=287, Evalue=9e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 52876; Mature: 52876

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERRVKITETVLRDAHQSLMATRMTTEEMLPIAEKLDKVGYHSLEVWGGATFDACLRFLN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHC
EDPWERLRELKKRIKNTPLQMLLRGQNLVGYRHYPDDIVEKFIEKAVANGIDIIRIFDAL
CCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
NDVRNLEVSIKATKRVGAHAQGTLVYTISPVHTIDHYIRVAKELVELGVDSICIKDMSGI
HHHHCCEEEEEEHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LSPYVAYELVKRLKETVNVPIQLHGHYTSGMASMTYLKAIEAGVDAIDTAISPLALGTSQ
HHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PATETMVAALKGTQYDTGLDLELLSEIASYFKEVKRNHYKETDFSMVMGIDTDVLVYQVP
CHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEECC
GGMLSNLIAQLKEQKALDKYKEVLEEIPRVREDLGYPPLVTPMSQMVGTQAVLNVITGER
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCH
YKMVPKEIKDYVKGLYGRPPAPISDEIKRKIIGDEEVIEIRPADLLKPQFEEVKEEIKEY
HHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCEEEECCHHHCCCHHHHHHHHHHHH
YEQEEDVLTYALFPQVAKKFFEYRRAKKYFIDSSMVGEGNRVYPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MERRVKITETVLRDAHQSLMATRMTTEEMLPIAEKLDKVGYHSLEVWGGATFDACLRFLN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHC
EDPWERLRELKKRIKNTPLQMLLRGQNLVGYRHYPDDIVEKFIEKAVANGIDIIRIFDAL
CCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
NDVRNLEVSIKATKRVGAHAQGTLVYTISPVHTIDHYIRVAKELVELGVDSICIKDMSGI
HHHHCCEEEEEEHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LSPYVAYELVKRLKETVNVPIQLHGHYTSGMASMTYLKAIEAGVDAIDTAISPLALGTSQ
HHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PATETMVAALKGTQYDTGLDLELLSEIASYFKEVKRNHYKETDFSMVMGIDTDVLVYQVP
CHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEECC
GGMLSNLIAQLKEQKALDKYKEVLEEIPRVREDLGYPPLVTPMSQMVGTQAVLNVITGER
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCH
YKMVPKEIKDYVKGLYGRPPAPISDEIKRKIIGDEEVIEIRPADLLKPQFEEVKEEIKEY
HHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCEEEECCHHHCCCHHHHHHHHHHHH
YEQEEDVLTYALFPQVAKKFFEYRRAKKYFIDSSMVGEGNRVYPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]