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The map label for this gene is dosC [H]

Identifier: 20807527

GI number: 20807527

Start: 1054674

End: 1055399

Strand: Direct

Name: dosC [H]

Synonym: TTE1052

Alternate gene names: 20807527

Gene position: 1054674-1055399 (Clockwise)

Preceding gene: 20807526

Following gene: 20807528

Centisome position: 39.22

GC content: 37.74

Gene sequence:

>726_bases
ATGACAAATCTCAAAGGTTTTATGATGGAATATAAAAATTACCTTTCAGAATACATTTTAAAAGGCGGACAGGATGAGAT
ACTGTTTGAGGCATACCAGCACCTTATAAAATTATTGGACGATTCTTCAGCTCAAGCCGCAAATATTTTAGATATTCATA
ATCAAGTTCTGAAAGACGTTCTTAATATAAAGCAGGATAACGATATGGTACAGTGGATTTACATTGAAAGAGCCACAGAA
TTTCTGGCCCAGATACTTATAGCAACAGACGCTTTACTCCTTTCTCTGAAAGAGAGTGTAGAAAGAGATCCATTGACAGG
GCTCTACAATAGATTGGCTATTGATAGAATTCTTTCGAAAGTTTGGCTGAATACAAATATGACCAAAACACCTCTTACAG
TAGCCATGCTTGATTTGGACGATTTTAAACATGTTAATGATAAATACGGACATCTGATCGGCGACGAGTTGTTGAAAGAA
GTGAGCACTGTTATAAAAGCTTGCCTCAGGGATAAAGATGTGGTTATGAGGTATGGAGGCGAGGAATTTCTCATATTGCT
GCCAGAAACAGAAATTGAGGGTGCCAAAAAAGCTCTTGAAAGGGTAAGGAAAAAAATAGAGGAAGGGATTTTTACAGAAG
CCAAGATTAAAGCTACTGCCAGCATAGGTGCGGCAGTATATCCGGATGATCGTCCTTTAAGTATGGAGCATTCCTGTAAT
GAATAA

Upstream 100 bases:

>100_bases
GAACATTAAGTCCTAGAGACGTTGCTTTAAAAATAATAAAGGAATTCAGCATAAAAGAAGATGATGCTACCGTACTAGTA
GCAAGGGGGGAAATGGGGTA

Downstream 100 bases:

>100_bases
AAAATAAGGAGGGTGCATAGTGTAAAATTATCGTAGGATTTTTTGAGTGAAAAAAACAAGAGACAACCCCTGTGATAAAA
TAGAACTTGGAAACAAAACA

Product: diguanylate cyclase/phosphodiesterase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MTNLKGFMMEYKNYLSEYILKGGQDEILFEAYQHLIKLLDDSSAQAANILDIHNQVLKDVLNIKQDNDMVQWIYIERATE
FLAQILIATDALLLSLKESVERDPLTGLYNRLAIDRILSKVWLNTNMTKTPLTVAMLDLDDFKHVNDKYGHLIGDELLKE
VSTVIKACLRDKDVVMRYGGEEFLILLPETEIEGAKKALERVRKKIEEGIFTEAKIKATASIGAAVYPDDRPLSMEHSCN
E

Sequences:

>Translated_241_residues
MTNLKGFMMEYKNYLSEYILKGGQDEILFEAYQHLIKLLDDSSAQAANILDIHNQVLKDVLNIKQDNDMVQWIYIERATE
FLAQILIATDALLLSLKESVERDPLTGLYNRLAIDRILSKVWLNTNMTKTPLTVAMLDLDDFKHVNDKYGHLIGDELLKE
VSTVIKACLRDKDVVMRYGGEEFLILLPETEIEGAKKALERVRKKIEEGIFTEAKIKATASIGAAVYPDDRPLSMEHSCN
E
>Mature_240_residues
TNLKGFMMEYKNYLSEYILKGGQDEILFEAYQHLIKLLDDSSAQAANILDIHNQVLKDVLNIKQDNDMVQWIYIERATEF
LAQILIATDALLLSLKESVERDPLTGLYNRLAIDRILSKVWLNTNMTKTPLTVAMLDLDDFKHVNDKYGHLIGDELLKEV
STVIKACLRDKDVVMRYGGEEFLILLPETEIEGAKKALERVRKKIEEGIFTEAKIKATASIGAAVYPDDRPLSMEHSCNE

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=207, Percent_Identity=33.8164251207729, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=35.3658536585366, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI87082007, Length=137, Percent_Identity=37.2262773722628, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI87081881, Length=130, Percent_Identity=33.8461538461538, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1787541, Length=131, Percent_Identity=37.4045801526718, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788381, Length=141, Percent_Identity=34.0425531914894, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1787816, Length=145, Percent_Identity=34.4827586206897, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1786584, Length=105, Percent_Identity=34.2857142857143, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787802, Length=141, Percent_Identity=34.0425531914894, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87081977, Length=104, Percent_Identity=33.6538461538462, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788085, Length=147, Percent_Identity=29.9319727891156, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87081974, Length=146, Percent_Identity=28.7671232876712, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 27466; Mature: 27335

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLKGFMMEYKNYLSEYILKGGQDEILFEAYQHLIKLLDDSSAQAANILDIHNQVLKDV
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LNIKQDNDMVQWIYIERATEFLAQILIATDALLLSLKESVERDPLTGLYNRLAIDRILSK
HCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VWLNTNMTKTPLTVAMLDLDDFKHVNDKYGHLIGDELLKEVSTVIKACLRDKDVVMRYGG
HHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCC
EEFLILLPETEIEGAKKALERVRKKIEEGIFTEAKIKATASIGAAVYPDDRPLSMEHSCN
CEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEECCCCCCCCCCCCCC
E
C
>Mature Secondary Structure 
TNLKGFMMEYKNYLSEYILKGGQDEILFEAYQHLIKLLDDSSAQAANILDIHNQVLKDV
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LNIKQDNDMVQWIYIERATEFLAQILIATDALLLSLKESVERDPLTGLYNRLAIDRILSK
HCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VWLNTNMTKTPLTVAMLDLDDFKHVNDKYGHLIGDELLKEVSTVIKACLRDKDVVMRYGG
HHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCC
EEFLILLPETEIEGAKKALERVRKKIEEGIFTEAKIKATASIGAAVYPDDRPLSMEHSCN
CEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEECCCCCCCCCCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]