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The map label for this gene is VacB [H]

Identifier: 20807463

GI number: 20807463

Start: 985795

End: 987921

Strand: Direct

Name: VacB [H]

Synonym: TTE0983

Alternate gene names: 20807463

Gene position: 985795-987921 (Clockwise)

Preceding gene: 20807462

Following gene: 20807464

Centisome position: 36.65

GC content: 38.79

Gene sequence:

>2127_bases
ATGAAGATAAAGGAACGGTTATTGCAACTTTTAAGAGAAGAAGATTACAAACCTTCCAAAATTGAGGAAATAATGGACAT
GCTGCACATAGACTACAACCAGAGAAAAATATTAGAAGCTACTTTGAAAGAAATGGAGAAAGAAGGACTGGTATTTAAAA
CTAAAAGAGGTAAATATGCCCTTCCAGAAAGGCTTGGCCTTGTAAGAGGGAGAATAGATGGACATCCTAGAGGGTATGGG
TTTTTAATTCCTGAAGAGCAGGGAATTAAAGACATATTTATACCTATTAGCGGGATGAACGGAGCAATGGATGGAGATCT
AGTTCTTGTAAGGGTCATAGAAGGTGCTGAAGGGAAAAGCCAAGAAGGCGAAGTAGTAAAAATTTTAAAAAGAGCTAATA
CGACAATTGTCGGAACTTACGAAAAAAATAAAAATTTTGGCTTTGTGATACCGGATAATAAAAAGATACATCAAGATGTA
TTCATTCCAAAAGGGGAAGATAAAGGCGCTAAAACTGGAATGAAAGTAGTAGTAAGAATTACTAAATGGCCTGAGGGAAG
AAGAAGTCCTGAAGGAGAAATAATAGAAGTTTTAGGGTACAAAGGAGATCCGGGGATAGACGTAAAGTCAATTTTGAGGT
CTTATGACATTCCGGAGACATTTCCTAAAGAGGTTCTAAAAGAAGCGGAAGAACTTCCAGAGGAGATACCGGAAAAAGAA
AAAAAGAGGCGGGTTGACCTCACAAAATTAAAATTTGTCACTATTGATGGAGAAGATGCGAAAGACTTGGACGACGCTGT
TTATGTGGAAAGACTTCCAAACGGCAATTATCTCTTGTATGTCAGTATAGCAGATGTCAGCCATTACGTTAAAGAAGGTA
CAAATCTTGACAAAGAAGCTCTTAGAAGGGGTTGTAGCGTTTACTTTCTCGACAGAGTGATACCTATGCTTCCTCCAAAG
CTTTCAAATGGCATATGCAGTTTGAATCCGGGAGAAGAAAGGTTGAGCCTCACGGTTAAAATGGAAATAAATACAAGGGG
AGAGATAGTAGACCACGATATTTTTGAGAGCATAATAGAGAGCAAAGAGAGAATGACTTACACCAGCGTTTATAAAATTC
TTGAAGAAAATGACGAAGAGCTGATAAAAAGGTATTCCCATCTTGTAGAAGACTTTAAGCTAATGAAAGAACTGGCACTT
GTGCTTTTAGAAAAAAGAAAGAGAAGGGGGAGTGTGGACTTTGACTTCCCGGAAGCTAAAGTGATTGTGGATGAAAAGGG
AAGACCTGTAGATATTGTAAAGGTAGAGAGAAATATTGCCCACAAAATAATTGAAGAATTCATGCTGGCAGCCAATGAGA
CGGTAGCTGAACACATGCATTGGTTAAACGTTCCTTTTGTCTACAGGATTCACGAGCATCCTGATATAGAGAAACTACTA
GCTTTTAATAAATTCATCCACAACCTGGGATATCATATAAAGGGAGTAGAAGGCGGAGAAATTCATCCCAAAGCTTTGCA
GGACCTCATAAGGCAGGTCAGAGGGAAAAGTGAACAAAAAGTAGTAGAGACTCTTCTCTTGCGCTCTTTGAAAAGAGCAA
GGTACAGTCCTGAAGACATAGGGCATTACGCTTTGGCAGCAAAGTATTACACTCATTTTACCTCTCCTATAAGGAGATAT
CCAGACCTTGTGATTCACAGGATAATAAAAGAATACATAAATGGAAAACTTACAGAAAAAAGGCAGAGACATTACAATAG
GATCCTTGAGGATATTGCAGCAAGAGCCTCAGAAAGGGAAAGAGCTGCGGAAGCCGCAGAAAGAGAGATAGAGGAGCTCA
AAAAAGTGGAATACATGGCAGATAAAGTAGGAAATGTGTACAAAGGCATAATTTCCAATGTCACCAGTTACGGCTTTTTT
GTAGAACTCGATAACACCGTTGAGGGATTGGTGGATGTAGCGTCATTGGAAGATGATTACTATGTATTTGACCCTGAAAG
ATATGTTCTGGTAGGGGAAAGGACAAAAAAGGTGTACTCTATAGGGAAAGAAGTTTACGTTAAAGTAGCCCATGTAGATG
TGGATAATAGGGAGATAGATTTTGTACTGGTAGAAGAAGATAGTTGA

Upstream 100 bases:

>100_bases
TGCGAAGAATTGGGGATTTCTTTAGATGAATTCTTGGAGATAGGTCTAAATTCCATGAAAGAAATTGCAGAACAAATAGG
ACTGTGAGGTATGAGGTGTA

Downstream 100 bases:

>100_bases
CAAATTTTTTGAAATAAATATAAAATAATATATGGAGAGATAATATATGGAAATGGGGGAGGTGGCAACGGTGCAAGTGA
TACTCTTGCGAGATGTTGCT

Product: exoribonuclease

Products: NA

Alternate protein names: RNase R; VacB protein homolog [H]

Number of amino acids: Translated: 708; Mature: 708

Protein sequence:

>708_residues
MKIKERLLQLLREEDYKPSKIEEIMDMLHIDYNQRKILEATLKEMEKEGLVFKTKRGKYALPERLGLVRGRIDGHPRGYG
FLIPEEQGIKDIFIPISGMNGAMDGDLVLVRVIEGAEGKSQEGEVVKILKRANTTIVGTYEKNKNFGFVIPDNKKIHQDV
FIPKGEDKGAKTGMKVVVRITKWPEGRRSPEGEIIEVLGYKGDPGIDVKSILRSYDIPETFPKEVLKEAEELPEEIPEKE
KKRRVDLTKLKFVTIDGEDAKDLDDAVYVERLPNGNYLLYVSIADVSHYVKEGTNLDKEALRRGCSVYFLDRVIPMLPPK
LSNGICSLNPGEERLSLTVKMEINTRGEIVDHDIFESIIESKERMTYTSVYKILEENDEELIKRYSHLVEDFKLMKELAL
VLLEKRKRRGSVDFDFPEAKVIVDEKGRPVDIVKVERNIAHKIIEEFMLAANETVAEHMHWLNVPFVYRIHEHPDIEKLL
AFNKFIHNLGYHIKGVEGGEIHPKALQDLIRQVRGKSEQKVVETLLLRSLKRARYSPEDIGHYALAAKYYTHFTSPIRRY
PDLVIHRIIKEYINGKLTEKRQRHYNRILEDIAARASERERAAEAAEREIEELKKVEYMADKVGNVYKGIISNVTSYGFF
VELDNTVEGLVDVASLEDDYYVFDPERYVLVGERTKKVYSIGKEVYVKVAHVDVDNREIDFVLVEEDS

Sequences:

>Translated_708_residues
MKIKERLLQLLREEDYKPSKIEEIMDMLHIDYNQRKILEATLKEMEKEGLVFKTKRGKYALPERLGLVRGRIDGHPRGYG
FLIPEEQGIKDIFIPISGMNGAMDGDLVLVRVIEGAEGKSQEGEVVKILKRANTTIVGTYEKNKNFGFVIPDNKKIHQDV
FIPKGEDKGAKTGMKVVVRITKWPEGRRSPEGEIIEVLGYKGDPGIDVKSILRSYDIPETFPKEVLKEAEELPEEIPEKE
KKRRVDLTKLKFVTIDGEDAKDLDDAVYVERLPNGNYLLYVSIADVSHYVKEGTNLDKEALRRGCSVYFLDRVIPMLPPK
LSNGICSLNPGEERLSLTVKMEINTRGEIVDHDIFESIIESKERMTYTSVYKILEENDEELIKRYSHLVEDFKLMKELAL
VLLEKRKRRGSVDFDFPEAKVIVDEKGRPVDIVKVERNIAHKIIEEFMLAANETVAEHMHWLNVPFVYRIHEHPDIEKLL
AFNKFIHNLGYHIKGVEGGEIHPKALQDLIRQVRGKSEQKVVETLLLRSLKRARYSPEDIGHYALAAKYYTHFTSPIRRY
PDLVIHRIIKEYINGKLTEKRQRHYNRILEDIAARASERERAAEAAEREIEELKKVEYMADKVGNVYKGIISNVTSYGFF
VELDNTVEGLVDVASLEDDYYVFDPERYVLVGERTKKVYSIGKEVYVKVAHVDVDNREIDFVLVEEDS
>Mature_708_residues
MKIKERLLQLLREEDYKPSKIEEIMDMLHIDYNQRKILEATLKEMEKEGLVFKTKRGKYALPERLGLVRGRIDGHPRGYG
FLIPEEQGIKDIFIPISGMNGAMDGDLVLVRVIEGAEGKSQEGEVVKILKRANTTIVGTYEKNKNFGFVIPDNKKIHQDV
FIPKGEDKGAKTGMKVVVRITKWPEGRRSPEGEIIEVLGYKGDPGIDVKSILRSYDIPETFPKEVLKEAEELPEEIPEKE
KKRRVDLTKLKFVTIDGEDAKDLDDAVYVERLPNGNYLLYVSIADVSHYVKEGTNLDKEALRRGCSVYFLDRVIPMLPPK
LSNGICSLNPGEERLSLTVKMEINTRGEIVDHDIFESIIESKERMTYTSVYKILEENDEELIKRYSHLVEDFKLMKELAL
VLLEKRKRRGSVDFDFPEAKVIVDEKGRPVDIVKVERNIAHKIIEEFMLAANETVAEHMHWLNVPFVYRIHEHPDIEKLL
AFNKFIHNLGYHIKGVEGGEIHPKALQDLIRQVRGKSEQKVVETLLLRSLKRARYSPEDIGHYALAAKYYTHFTSPIRRY
PDLVIHRIIKEYINGKLTEKRQRHYNRILEDIAARASERERAAEAAEREIEELKKVEYMADKVGNVYKGIISNVTSYGFF
VELDNTVEGLVDVASLEDDYYVFDPERYVLVGERTKKVYSIGKEVYVKVAHVDVDNREIDFVLVEEDS

Specific function: 3'-5'exoribonuclease that participates in an essential cell function. Acts nonspecifically on poly(A), poly(U) and ribosomal RNAs [H]

COG id: COG0557

COG function: function code K; Exoribonuclease R

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI190014625, Length=511, Percent_Identity=29.9412915851272, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI190014623, Length=511, Percent_Identity=29.9412915851272, Blast_Score=207, Evalue=3e-53,
Organism=Homo sapiens, GI134288890, Length=414, Percent_Identity=30.9178743961353, Blast_Score=185, Evalue=1e-46,
Organism=Homo sapiens, GI19115966, Length=622, Percent_Identity=26.0450160771704, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI219521928, Length=638, Percent_Identity=25.8620689655172, Blast_Score=163, Evalue=6e-40,
Organism=Homo sapiens, GI156105695, Length=324, Percent_Identity=25.6172839506173, Blast_Score=70, Evalue=7e-12,
Organism=Homo sapiens, GI156105693, Length=214, Percent_Identity=29.4392523364486, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI87082383, Length=700, Percent_Identity=40.2857142857143, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI1787542, Length=639, Percent_Identity=27.5430359937402, Blast_Score=190, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI212645896, Length=546, Percent_Identity=28.5714285714286, Blast_Score=175, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17553506, Length=536, Percent_Identity=27.9850746268657, Blast_Score=174, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6324552, Length=563, Percent_Identity=29.1296625222025, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6320499, Length=419, Percent_Identity=26.2529832935561, Blast_Score=118, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6323943, Length=429, Percent_Identity=24.4755244755245, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24649634, Length=488, Percent_Identity=29.5081967213115, Blast_Score=215, Evalue=7e-56,
Organism=Drosophila melanogaster, GI24654592, Length=469, Percent_Identity=27.9317697228145, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI19922976, Length=469, Percent_Identity=27.9317697228145, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24654597, Length=469, Percent_Identity=27.9317697228145, Blast_Score=167, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013223
- InterPro:   IPR001900
- InterPro:   IPR022966
- InterPro:   IPR004476
- InterPro:   IPR011805 [H]

Pfam domain/function: PF08206 OB_RNB; PF00773 RNB; PF00575 S1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 81675; Mature: 81675

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50126 S1 ; PS01175 RIBONUCLEASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKERLLQLLREEDYKPSKIEEIMDMLHIDYNQRKILEATLKEMEKEGLVFKTKRGKYA
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEECCCCCC
LPERLGLVRGRIDGHPRGYGFLIPEEQGIKDIFIPISGMNGAMDGDLVLVRVIEGAEGKS
CHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCC
QEGEVVKILKRANTTIVGTYEKNKNFGFVIPDNKKIHQDVFIPKGEDKGAKTGMKVVVRI
CCCHHHHHHHHCCCEEEEEEECCCCEEEEECCCCCHHHCCCCCCCCCCCCCCCEEEEEEE
TKWPEGRRSPEGEIIEVLGYKGDPGIDVKSILRSYDIPETFPKEVLKEAEELPEEIPEKE
ECCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHH
KKRRVDLTKLKFVTIDGEDAKDLDDAVYVERLPNGNYLLYVSIADVSHYVKEGTNLDKEA
HHHCCCEEEEEEEEECCCCCCCCHHHHHHHCCCCCCEEEEEEEHHHHHHHHCCCCCCHHH
LRRGCSVYFLDRVIPMLPPKLSNGICSLNPGEERLSLTVKMEINTRGEIVDHDIFESIIE
HHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHH
SKERMTYTSVYKILEENDEELIKRYSHLVEDFKLMKELALVLLEKRKRRGSVDFDFPEAK
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
VIVDEKGRPVDIVKVERNIAHKIIEEFMLAANETVAEHMHWLNVPFVYRIHEHPDIEKLL
EEECCCCCCEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCCHHHHH
AFNKFIHNLGYHIKGVEGGEIHPKALQDLIRQVRGKSEQKVVETLLLRSLKRARYSPEDI
HHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHH
GHYALAAKYYTHFTSPIRRYPDLVIHRIIKEYINGKLTEKRQRHYNRILEDIAARASERE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
RAAEAAEREIEELKKVEYMADKVGNVYKGIISNVTSYGFFVELDNTVEGLVDVASLEDDY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHCCCCE
YVFDPERYVLVGERTKKVYSIGKEVYVKVAHVDVDNREIDFVLVEEDS
EEECCCEEEEECCHHHHHHHCCHHHEEEEEEEECCCCEEEEEEEECCC
>Mature Secondary Structure
MKIKERLLQLLREEDYKPSKIEEIMDMLHIDYNQRKILEATLKEMEKEGLVFKTKRGKYA
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEECCCCCC
LPERLGLVRGRIDGHPRGYGFLIPEEQGIKDIFIPISGMNGAMDGDLVLVRVIEGAEGKS
CHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCC
QEGEVVKILKRANTTIVGTYEKNKNFGFVIPDNKKIHQDVFIPKGEDKGAKTGMKVVVRI
CCCHHHHHHHHCCCEEEEEEECCCCEEEEECCCCCHHHCCCCCCCCCCCCCCCEEEEEEE
TKWPEGRRSPEGEIIEVLGYKGDPGIDVKSILRSYDIPETFPKEVLKEAEELPEEIPEKE
ECCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHH
KKRRVDLTKLKFVTIDGEDAKDLDDAVYVERLPNGNYLLYVSIADVSHYVKEGTNLDKEA
HHHCCCEEEEEEEEECCCCCCCCHHHHHHHCCCCCCEEEEEEEHHHHHHHHCCCCCCHHH
LRRGCSVYFLDRVIPMLPPKLSNGICSLNPGEERLSLTVKMEINTRGEIVDHDIFESIIE
HHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHH
SKERMTYTSVYKILEENDEELIKRYSHLVEDFKLMKELALVLLEKRKRRGSVDFDFPEAK
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
VIVDEKGRPVDIVKVERNIAHKIIEEFMLAANETVAEHMHWLNVPFVYRIHEHPDIEKLL
EEECCCCCCEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCCHHHHH
AFNKFIHNLGYHIKGVEGGEIHPKALQDLIRQVRGKSEQKVVETLLLRSLKRARYSPEDI
HHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHH
GHYALAAKYYTHFTSPIRRYPDLVIHRIIKEYINGKLTEKRQRHYNRILEDIAARASERE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
RAAEAAEREIEELKKVEYMADKVGNVYKGIISNVTSYGFFVELDNTVEGLVDVASLEDDY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHCCCCE
YVFDPERYVLVGERTKKVYSIGKEVYVKVAHVDVDNREIDFVLVEEDS
EEECCCEEEEECCHHHHHHHCCHHHEEEEEEEECCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]