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The map label for this gene is LepA [H]

Identifier: 20807432

GI number: 20807432

Start: 954197

End: 956008

Strand: Direct

Name: LepA [H]

Synonym: TTE0951

Alternate gene names: 20807432

Gene position: 954197-956008 (Clockwise)

Preceding gene: 20807431

Following gene: 20807433

Centisome position: 35.48

GC content: 42.33

Gene sequence:

>1812_bases
ATGTCTGAAAGGATGAAGTACATTCGCAATTTCTGCATCATTGCCCATATTGACCATGGGAAGTCAACTTTAGCGGACAG
GCTCATTGAAAAGACAGGGTTTTTGTCTGAAAGGGAAATGGACAAGCAAATTTTGGACAACTTAGAGCTTGAAAGAGAAA
GGGGCATTACAATAAAATTAAAGCCTGTGAGGATGATTTACAAGGCCAAAGACGGCAACGAATACGAGCTAAATTTGATA
GATACGCCGGGACACGTAGATTTTACCTATGAGGTTTCTCGAAGCATTGCCGCTTGTGAAGGAGCGCTGCTTGTAGTGGA
TGCAAGCCAGGGCATTGAAGCCCAGACTTTGGCTAACGTTTACCTGGCTCTTGAGCACGACCTTGAGATAATACCTGTTA
TAAATAAAATTGACCTTCCATCTGCCGACCCCGAAAGGGTGAAAAGAGAGATTGAAGATATCATAGGAATAGATGCGAGT
GAAGCGCTTTTAACTTCTGCTAAAGAGGGAATTGGGATAGAGGAAGTTTTAGAAGCGATTGTGAAAAGAATACCGCCTCC
CCAGGGAGATGAGGACAAGCCTTTAAAAGCTTTAATTTTTGATTCTTTCTACGACAACTACAAGGGAGCCATAAGCTTTG
TCAGAATAGTTGACGGTAAAGTTAAACGGGGCATGAGGATAAAGATGTTTTCTACAGGAAAAGTGTTTGAAGTGACAGAA
GTTGGGAGTTTCAGACCCGACCTTTATCCTGTGGAGGAATTAAAAGCAGGAGAAGTAGGATACATTGCGGCCAATATAAA
AAATGTGAAGGATACCCGCGTTGGAGACACAATAACTGACGCTGATAATCCGGCGGATTCGCCTCTTCCGGGTTACAAAG
AGGTCGTCCCAATGGTGTTTTGCGGAATTTATCCAGCTGACGGGGAGGACTATGAAAATCTAAAAGAGGCTTTAGAGAAA
CTTCAATTAAATGATGCTTCACTGGTGTTTGAGCCAGATACTTCTGCTGCTTTGGGTTTTGGATTCAGATGCGGTTTTTT
AGGACTTCTTCACATGGAAATCGTTCAGGAGAGGTTAGAGAGGGAGTACAATTTAAACCTTGTGACGACTGCTCCCAGCG
TAATTTACAAGGTCTACAAGACTAATGGAGAAGTGCTGGAGCTTGACAACCCTACGAAAATGCCTCCTCCAACCCAGATT
GACCACATTGAAGAGCCTATTGTAGAGGCCACAATAATGGTGCCAACAGACTACGTAGGACCAGTCATGGAACTTTGTCA
GGAAAGAAGGGGAGTCTATCTCGGGATGGAGTACATTGAAAAAACGAGAGTGCTTTTAAAGTATGAAATGCCTCTCAATG
AGATAATCTATGATTTTTTTGATGCTTTAAAGTCAAGGACTAGAGGATACGCTTCTTTGGATTATGAATTTAAAGGATAT
AAGAAGTCGGACCTTGTGAAGCTGGACATCTTGGTAAATGGCGAGGTTGTAGATGCTCTTTCCATGATAGTTCACAAGGA
CAAGGCGTACGAAAAAGGAAGGAAAATAGTTGAAAAGTTAAAGGAGAACATTCCGAGACATTTATTCGAAATTCCAATAC
AGGCTGCAATTGGGTCTAGAATCATAGCAAGAGAGACGGTAAAGGCTTTGAGGAAGAATGTGCTGGCGAAGTGCTATGGC
GGTGACGTCACGCGAAAGAAAAAGCTTTTGGAGAAGCAGAAGGAAGGTAAAAAGAGGATGAGACAAATAGGGACTGTGGA
AATACCGCAGGAAGCTTTTATGTCAATTTTAAAGTTGGATGACCAAAAATGA

Upstream 100 bases:

>100_bases
GAATTTTTAAAATAGAGCTTTTTGACGAAAAAATATTCCTTAAAGTCTTGGGAAAGGAGTTTATAATCAGGAAATAGCGC
TATTTTAGAGGGGGAATAAA

Downstream 100 bases:

>100_bases
GGGAAATAGGTATATACATTCACATTCCTTTTTGCAAGAAAAAATGCTATTACTGTGATTTTAATTCTTACGCCGGCTAT
GAACACTTGATGGAGGACTA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 603; Mature: 602

Protein sequence:

>603_residues
MSERMKYIRNFCIIAHIDHGKSTLADRLIEKTGFLSEREMDKQILDNLELERERGITIKLKPVRMIYKAKDGNEYELNLI
DTPGHVDFTYEVSRSIAACEGALLVVDASQGIEAQTLANVYLALEHDLEIIPVINKIDLPSADPERVKREIEDIIGIDAS
EALLTSAKEGIGIEEVLEAIVKRIPPPQGDEDKPLKALIFDSFYDNYKGAISFVRIVDGKVKRGMRIKMFSTGKVFEVTE
VGSFRPDLYPVEELKAGEVGYIAANIKNVKDTRVGDTITDADNPADSPLPGYKEVVPMVFCGIYPADGEDYENLKEALEK
LQLNDASLVFEPDTSAALGFGFRCGFLGLLHMEIVQERLEREYNLNLVTTAPSVIYKVYKTNGEVLELDNPTKMPPPTQI
DHIEEPIVEATIMVPTDYVGPVMELCQERRGVYLGMEYIEKTRVLLKYEMPLNEIIYDFFDALKSRTRGYASLDYEFKGY
KKSDLVKLDILVNGEVVDALSMIVHKDKAYEKGRKIVEKLKENIPRHLFEIPIQAAIGSRIIARETVKALRKNVLAKCYG
GDVTRKKKLLEKQKEGKKRMRQIGTVEIPQEAFMSILKLDDQK

Sequences:

>Translated_603_residues
MSERMKYIRNFCIIAHIDHGKSTLADRLIEKTGFLSEREMDKQILDNLELERERGITIKLKPVRMIYKAKDGNEYELNLI
DTPGHVDFTYEVSRSIAACEGALLVVDASQGIEAQTLANVYLALEHDLEIIPVINKIDLPSADPERVKREIEDIIGIDAS
EALLTSAKEGIGIEEVLEAIVKRIPPPQGDEDKPLKALIFDSFYDNYKGAISFVRIVDGKVKRGMRIKMFSTGKVFEVTE
VGSFRPDLYPVEELKAGEVGYIAANIKNVKDTRVGDTITDADNPADSPLPGYKEVVPMVFCGIYPADGEDYENLKEALEK
LQLNDASLVFEPDTSAALGFGFRCGFLGLLHMEIVQERLEREYNLNLVTTAPSVIYKVYKTNGEVLELDNPTKMPPPTQI
DHIEEPIVEATIMVPTDYVGPVMELCQERRGVYLGMEYIEKTRVLLKYEMPLNEIIYDFFDALKSRTRGYASLDYEFKGY
KKSDLVKLDILVNGEVVDALSMIVHKDKAYEKGRKIVEKLKENIPRHLFEIPIQAAIGSRIIARETVKALRKNVLAKCYG
GDVTRKKKLLEKQKEGKKRMRQIGTVEIPQEAFMSILKLDDQK
>Mature_602_residues
SERMKYIRNFCIIAHIDHGKSTLADRLIEKTGFLSEREMDKQILDNLELERERGITIKLKPVRMIYKAKDGNEYELNLID
TPGHVDFTYEVSRSIAACEGALLVVDASQGIEAQTLANVYLALEHDLEIIPVINKIDLPSADPERVKREIEDIIGIDASE
ALLTSAKEGIGIEEVLEAIVKRIPPPQGDEDKPLKALIFDSFYDNYKGAISFVRIVDGKVKRGMRIKMFSTGKVFEVTEV
GSFRPDLYPVEELKAGEVGYIAANIKNVKDTRVGDTITDADNPADSPLPGYKEVVPMVFCGIYPADGEDYENLKEALEKL
QLNDASLVFEPDTSAALGFGFRCGFLGLLHMEIVQERLEREYNLNLVTTAPSVIYKVYKTNGEVLELDNPTKMPPPTQID
HIEEPIVEATIMVPTDYVGPVMELCQERRGVYLGMEYIEKTRVLLKYEMPLNEIIYDFFDALKSRTRGYASLDYEFKGYK
KSDLVKLDILVNGEVVDALSMIVHKDKAYEKGRKIVEKLKENIPRHLFEIPIQAAIGSRIIARETVKALRKNVLAKCYGG
DVTRKKKLLEKQKEGKKRMRQIGTVEIPQEAFMSILKLDDQK

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=604, Percent_Identity=52.9801324503311, Blast_Score=671, Evalue=0.0,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=46.969696969697, Blast_Score=120, Evalue=5e-27,
Organism=Homo sapiens, GI4503483, Length=143, Percent_Identity=39.1608391608392, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI18390331, Length=169, Percent_Identity=34.3195266272189, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI25306287, Length=134, Percent_Identity=44.0298507462687, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI19923640, Length=134, Percent_Identity=44.0298507462687, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI25306283, Length=134, Percent_Identity=44.0298507462687, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI310132016, Length=109, Percent_Identity=44.954128440367, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI310110807, Length=109, Percent_Identity=44.954128440367, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI310123363, Length=109, Percent_Identity=44.954128440367, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI217272894, Length=155, Percent_Identity=34.8387096774194, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI217272892, Length=155, Percent_Identity=34.8387096774194, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI4503471, Length=339, Percent_Identity=25.9587020648968, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI53729339, Length=219, Percent_Identity=26.027397260274, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI53729337, Length=219, Percent_Identity=26.027397260274, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI94966752, Length=83, Percent_Identity=42.1686746987952, Blast_Score=70, Evalue=7e-12,
Organism=Escherichia coli, GI1788922, Length=595, Percent_Identity=60, Blast_Score=731, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=499, Percent_Identity=30.6613226452906, Blast_Score=175, Evalue=8e-45,
Organism=Escherichia coli, GI1789738, Length=178, Percent_Identity=34.2696629213483, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1790835, Length=159, Percent_Identity=30.188679245283, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1789559, Length=228, Percent_Identity=28.9473684210526, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1789108, Length=365, Percent_Identity=24.3835616438356, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1789737, Length=287, Percent_Identity=29.2682926829268, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790412, Length=287, Percent_Identity=29.2682926829268, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17557151, Length=610, Percent_Identity=42.1311475409836, Blast_Score=508, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17556745, Length=461, Percent_Identity=26.6811279826464, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=38.8059701492537, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=38.8059701492537, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17552882, Length=145, Percent_Identity=35.8620689655172, Blast_Score=96, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=162, Percent_Identity=33.3333333333333, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=143, Percent_Identity=35.6643356643357, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32566303, Length=319, Percent_Identity=26.9592476489028, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323320, Length=606, Percent_Identity=47.6897689768977, Blast_Score=550, Evalue=1e-157,
Organism=Saccharomyces cerevisiae, GI6324707, Length=150, Percent_Identity=38, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=150, Percent_Identity=38, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323098, Length=187, Percent_Identity=34.2245989304813, Blast_Score=103, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=40, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=141, Percent_Identity=38.2978723404255, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6325337, Length=332, Percent_Identity=25.9036144578313, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319594, Length=332, Percent_Identity=25.9036144578313, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=274, Percent_Identity=29.1970802919708, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI78706572, Length=599, Percent_Identity=47.7462437395659, Blast_Score=585, Evalue=1e-167,
Organism=Drosophila melanogaster, GI28574573, Length=143, Percent_Identity=44.0559440559441, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24582462, Length=180, Percent_Identity=36.6666666666667, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI221458488, Length=149, Percent_Identity=40.9395973154362, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24585709, Length=154, Percent_Identity=34.4155844155844, Blast_Score=96, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24585711, Length=152, Percent_Identity=34.8684210526316, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585713, Length=152, Percent_Identity=34.8684210526316, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21357743, Length=160, Percent_Identity=33.75, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281363316, Length=297, Percent_Identity=28.2828282828283, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17864358, Length=297, Percent_Identity=28.2828282828283, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19921738, Length=288, Percent_Identity=26.3888888888889, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 68167; Mature: 68035

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERMKYIRNFCIIAHIDHGKSTLADRLIEKTGFLSEREMDKQILDNLELERERGITIKL
CCHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHCCCEEEE
KPVRMIYKAKDGNEYELNLIDTPGHVDFTYEVSRSIAACEGALLVVDASQGIEAQTLANV
EEEEEEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHH
YLALEHDLEIIPVINKIDLPSADPERVKREIEDIIGIDASEALLTSAKEGIGIEEVLEAI
HEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHH
VKRIPPPQGDEDKPLKALIFDSFYDNYKGAISFVRIVDGKVKRGMRIKMFSTGKVFEVTE
HHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCHHHCCCCEEEEECCCCEEEEEE
VGSFRPDLYPVEELKAGEVGYIAANIKNVKDTRVGDTITDADNPADSPLPGYKEVVPMVF
CCCCCCCCCCHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
CGIYPADGEDYENLKEALEKLQLNDASLVFEPDTSAALGFGFRCGFLGLLHMEIVQERLE
HCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHCCCHHHHHHHHHHHHHHHHHHH
REYNLNLVTTAPSVIYKVYKTNGEVLELDNPTKMPPPTQIDHIEEPIVEATIMVPTDYVG
HHCCCEEEECCHHHHHHHEECCCCEEECCCCCCCCCCCCHHHHHHCHHEEEEECCCHHHH
PVMELCQERRGVYLGMEYIEKTRVLLKYEMPLNEIIYDFFDALKSRTRGYASLDYEFKGY
HHHHHHHHHCCEEEEHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHCCCCEEECCEECCC
KKSDLVKLDILVNGEVVDALSMIVHKDKAYEKGRKIVEKLKENIPRHLFEIPIQAAIGSR
CCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCH
IIARETVKALRKNVLAKCYGGDVTRKKKLLEKQKEGKKRMRQIGTVEIPQEAFMSILKLD
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
DQK
CCC
>Mature Secondary Structure 
SERMKYIRNFCIIAHIDHGKSTLADRLIEKTGFLSEREMDKQILDNLELERERGITIKL
CHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHCCCEEEE
KPVRMIYKAKDGNEYELNLIDTPGHVDFTYEVSRSIAACEGALLVVDASQGIEAQTLANV
EEEEEEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHH
YLALEHDLEIIPVINKIDLPSADPERVKREIEDIIGIDASEALLTSAKEGIGIEEVLEAI
HEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHH
VKRIPPPQGDEDKPLKALIFDSFYDNYKGAISFVRIVDGKVKRGMRIKMFSTGKVFEVTE
HHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCHHHCCCCEEEEECCCCEEEEEE
VGSFRPDLYPVEELKAGEVGYIAANIKNVKDTRVGDTITDADNPADSPLPGYKEVVPMVF
CCCCCCCCCCHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
CGIYPADGEDYENLKEALEKLQLNDASLVFEPDTSAALGFGFRCGFLGLLHMEIVQERLE
HCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHCCCHHHHHHHHHHHHHHHHHHH
REYNLNLVTTAPSVIYKVYKTNGEVLELDNPTKMPPPTQIDHIEEPIVEATIMVPTDYVG
HHCCCEEEECCHHHHHHHEECCCCEEECCCCCCCCCCCCHHHHHHCHHEEEEECCCHHHH
PVMELCQERRGVYLGMEYIEKTRVLLKYEMPLNEIIYDFFDALKSRTRGYASLDYEFKGY
HHHHHHHHHCCEEEEHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHCCCCEEECCEECCC
KKSDLVKLDILVNGEVVDALSMIVHKDKAYEKGRKIVEKLKENIPRHLFEIPIQAAIGSR
CCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCH
IIARETVKALRKNVLAKCYGGDVTRKKKLLEKQKEGKKRMRQIGTVEIPQEAFMSILKLD
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
DQK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA