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The map label for this gene is alaXL [H]

Identifier: 20807415

GI number: 20807415

Start: 938461

End: 939717

Strand: Direct

Name: alaXL [H]

Synonym: TTE0934

Alternate gene names: 20807415

Gene position: 938461-939717 (Clockwise)

Preceding gene: 20807414

Following gene: 20807416

Centisome position: 34.89

GC content: 36.99

Gene sequence:

>1257_bases
ATGAATAAGATTGCGCATCCTGTGCAATCTTATTCATACAGCGAAAGGGTGAAGAGGATGGATAAGCTTTTTTATGAGGA
TAGCTATGTTAAAGAATTTGAAGCTGTCATTTTAAAGAGAGAAGAAAAAGATGGAGAAAAAACTGTAGTTTTAGATAGAA
CTTATTTCTATCCAGAGGGCGGCGGACAGCCTTCTGACACTGGGAAGATAGGAAGTAAAAGTGTAATAAAGGCTTATGAG
AAAAAAGGAGAGGTTTACCACGTAGTAGAGGATTTCCCAGAAGGTGAAAGAGTCCACTGTGTGATAGATTGGGACAGGCG
ATTTGACCATATGCAGCAGCACACAGGACAGCACCTTCTGTCTTCTGTTTTGCTGAAAAAATATGGCGCAGATACAGAGA
GTTTTTCTATAGGTGAAAATTCATCTCATATTACAGTTACAAAAGAGTTCTTTACTGAAGAAGAAATTCACGAGGTAGAA
AAAGAAGTTAATGGACTCATATATAAAAATCTTGCAGTGAAAAGCTACTTTGCAGATGAAAAGCTTATTTCTAAATTGCC
TTTAAGGAAAAAGCCTCAAGTGAAGGAAAATATAAGGATAGTGGAGATAGAAGGAATTGATTATTCTCCTTGCGGAGGTA
CTCATGTAAAAAATACGGGAGAGATTGGAATTCTAAAAATAAAAAAGTGGGAAAGGACAAATAAAGGGGCAAGGCTTGAA
TTTGTTTGCGGTTTTAGAGCATTAGAAGATTATCAGAAAAAGAATAGTTTGGTAAATTTCTTGATACAGGTGCTTTCTGA
GAAGGAAGAAGGTATTGCAGAACATTTGCAGAAATTGCTTGAAGATTACAGGGAAGCAAAAAGAGAGGTAAATTTCTTAA
AAGAAAAGATATTAAAGGAAGAAGCAGAAAATCTGGTTAAAAAATCTGAACTGTACGGTGGAATTCACATTGTTTACAGC
TTATACGAGGGAAGAGACCCGGAAGATTTAAAAATGCTGGGCAAATTTGTAACTGCTGAAAGCAAGTCTGTAGCCATTTT
AGGCAATAAAAGAGGGGATGTCGCAGATATCGTCGTAGCAAGGTCTTATGACGTAGATATAGTAGTAAATGGTTTTTTTA
AGGAAGTATTGAGGACCTTTGATGGGAAAGGTGGAGGAAGTGCGCAGTTTTGTCAAGGTGGAGGTTCCCCATCTCGATTG
GAATTGGCAATGGACAAGGCACTGAAGTTTGTAATTGAGGAAATAAATAAAATGTAA

Upstream 100 bases:

>100_bases
GTGGACCACAGAGTTATAGGAGACGGAAAACCAGGACCTATAACCAAGAAAATAATAGAGGAATTTAGAAAGCTTACAAA
AGTTTTGGGCACAAAGGTGT

Downstream 100 bases:

>100_bases
AAAATTCTAAAAAGTAGAAGGAAAATTATAACATATATAGAATATATTATATAACGCAAAACGCTAATTCTAAAAAGAGA
TGGGGAGATAAGGATGGATA

Product: metal-dependent hydrolase

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-L; Alanyl-tRNA deacylase AlaX-L [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MNKIAHPVQSYSYSERVKRMDKLFYEDSYVKEFEAVILKREEKDGEKTVVLDRTYFYPEGGGQPSDTGKIGSKSVIKAYE
KKGEVYHVVEDFPEGERVHCVIDWDRRFDHMQQHTGQHLLSSVLLKKYGADTESFSIGENSSHITVTKEFFTEEEIHEVE
KEVNGLIYKNLAVKSYFADEKLISKLPLRKKPQVKENIRIVEIEGIDYSPCGGTHVKNTGEIGILKIKKWERTNKGARLE
FVCGFRALEDYQKKNSLVNFLIQVLSEKEEGIAEHLQKLLEDYREAKREVNFLKEKILKEEAENLVKKSELYGGIHIVYS
LYEGRDPEDLKMLGKFVTAESKSVAILGNKRGDVADIVVARSYDVDIVVNGFFKEVLRTFDGKGGGSAQFCQGGGSPSRL
ELAMDKALKFVIEEINKM

Sequences:

>Translated_418_residues
MNKIAHPVQSYSYSERVKRMDKLFYEDSYVKEFEAVILKREEKDGEKTVVLDRTYFYPEGGGQPSDTGKIGSKSVIKAYE
KKGEVYHVVEDFPEGERVHCVIDWDRRFDHMQQHTGQHLLSSVLLKKYGADTESFSIGENSSHITVTKEFFTEEEIHEVE
KEVNGLIYKNLAVKSYFADEKLISKLPLRKKPQVKENIRIVEIEGIDYSPCGGTHVKNTGEIGILKIKKWERTNKGARLE
FVCGFRALEDYQKKNSLVNFLIQVLSEKEEGIAEHLQKLLEDYREAKREVNFLKEKILKEEAENLVKKSELYGGIHIVYS
LYEGRDPEDLKMLGKFVTAESKSVAILGNKRGDVADIVVARSYDVDIVVNGFFKEVLRTFDGKGGGSAQFCQGGGSPSRL
ELAMDKALKFVIEEINKM
>Mature_418_residues
MNKIAHPVQSYSYSERVKRMDKLFYEDSYVKEFEAVILKREEKDGEKTVVLDRTYFYPEGGGQPSDTGKIGSKSVIKAYE
KKGEVYHVVEDFPEGERVHCVIDWDRRFDHMQQHTGQHLLSSVLLKKYGADTESFSIGENSSHITVTKEFFTEEEIHEVE
KEVNGLIYKNLAVKSYFADEKLISKLPLRKKPQVKENIRIVEIEGIDYSPCGGTHVKNTGEIGILKIKKWERTNKGARLE
FVCGFRALEDYQKKNSLVNFLIQVLSEKEEGIAEHLQKLLEDYREAKREVNFLKEKILKEEAENLVKKSELYGGIHIVYS
LYEGRDPEDLKMLGKFVTAESKSVAILGNKRGDVADIVVARSYDVDIVVNGFFKEVLRTFDGKGGGSAQFCQGGGSPSRL
ELAMDKALKFVIEEINKM

Specific function: Functions in trans to edit the amino acid moiety from mischarged charged tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-L subfamily [H]

Homologues:

Organism=Homo sapiens, GI217416402, Length=387, Percent_Identity=28.6821705426357, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI13376886, Length=387, Percent_Identity=28.6821705426357, Blast_Score=132, Evalue=6e-31,
Organism=Escherichia coli, GI1789048, Length=369, Percent_Identity=26.0162601626016, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324288, Length=296, Percent_Identity=31.7567567567568, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI18543305, Length=301, Percent_Identity=30.8970099667774, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24582809, Length=288, Percent_Identity=27.4305555555556, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45552267, Length=288, Percent_Identity=27.4305555555556, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 47720; Mature: 47720

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIAHPVQSYSYSERVKRMDKLFYEDSYVKEFEAVILKREEKDGEKTVVLDRTYFYPEG
CCCCCCHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCC
GGQPSDTGKIGSKSVIKAYEKKGEVYHVVEDFPEGERVHCVIDWDRRFDHMQQHTGQHLL
CCCCCCCCCCCCHHHHHHHHHCCCEEEEHHCCCCCCEEEEEEECHHHHHHHHHHHHHHHH
SSVLLKKYGADTESFSIGENSSHITVTKEFFTEEEIHEVEKEVNGLIYKNLAVKSYFADE
HHHHHHHHCCCCCCEECCCCCCEEEEEHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
KLISKLPLRKKPQVKENIRIVEIEGIDYSPCGGTHVKNTGEIGILKIKKWERTNKGARLE
HHHHHCCCCCCCCHHHCEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEE
FVCGFRALEDYQKKNSLVNFLIQVLSEKEEGIAEHLQKLLEDYREAKREVNFLKEKILKE
EEECHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAENLVKKSELYGGIHIVYSLYEGRDPEDLKMLGKFVTAESKSVAILGNKRGDVADIVVA
HHHHHHHHHHHCCCHHEEEEHHCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEE
RSYDVDIVVNGFFKEVLRTFDGKGGGSAQFCQGGGSPSRLELAMDKALKFVIEEINKM
ECCCCEEEEHHHHHHHHHHHCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKIAHPVQSYSYSERVKRMDKLFYEDSYVKEFEAVILKREEKDGEKTVVLDRTYFYPEG
CCCCCCHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCC
GGQPSDTGKIGSKSVIKAYEKKGEVYHVVEDFPEGERVHCVIDWDRRFDHMQQHTGQHLL
CCCCCCCCCCCCHHHHHHHHHCCCEEEEHHCCCCCCEEEEEEECHHHHHHHHHHHHHHHH
SSVLLKKYGADTESFSIGENSSHITVTKEFFTEEEIHEVEKEVNGLIYKNLAVKSYFADE
HHHHHHHHCCCCCCEECCCCCCEEEEEHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
KLISKLPLRKKPQVKENIRIVEIEGIDYSPCGGTHVKNTGEIGILKIKKWERTNKGARLE
HHHHHCCCCCCCCHHHCEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEE
FVCGFRALEDYQKKNSLVNFLIQVLSEKEEGIAEHLQKLLEDYREAKREVNFLKEKILKE
EEECHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAENLVKKSELYGGIHIVYSLYEGRDPEDLKMLGKFVTAESKSVAILGNKRGDVADIVVA
HHHHHHHHHHHCCCHHEEEEHHCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEE
RSYDVDIVVNGFFKEVLRTFDGKGGGSAQFCQGGGSPSRLELAMDKALKFVIEEINKM
ECCCCEEEEHHHHHHHHHHHCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]