Click here to switch to the map view.

The map label for this gene is IlvE [H]

Identifier: 20807414

GI number: 20807414

Start: 937593

End: 938468

Strand: Direct

Name: IlvE [H]

Synonym: TTE0933

Alternate gene names: 20807414

Gene position: 937593-938468 (Clockwise)

Preceding gene: 20807413

Following gene: 20807415

Centisome position: 34.86

GC content: 38.13

Gene sequence:

>876_bases
ATGGGATTAGTATATGTAAATGGAGAGTTTGTGGACAGCGAAAAAGCTTGTGTATCAGTTTTTGACCATGGCTACCTTTA
TGGGGACGGAATTTTTGAGGGGATAAGGGTTTACGACGGAGTCATATTTAAATTGGATGAGCATTTAAAGAGGCTTTACA
GTATGGCAAAAGTGCTTCTTTTGGACATTCCTCTTTCTATGGAAGAGATGAAAGAAAAAGTCGTGGAAACAGTGAGAATT
AATAATTTAAGAGATGCTTACATAAGGTTGGTTGTCTCAAGAGGAAAAGGGGATTTAGGACTTGACCCTTATAAATGTCC
TAAACCTACAGTCGTGATAATTGCAGATAAAATCGCCTTGTATCCAGAATCTATGTATGAGACGGGTCTTAAAATTATAA
CATCTTCCTTTAGAAGAAATTCAATACAGTCATTGGACCCGCAAATAAAGTCTTTGAACTATTTAAACAACATCTTAGCT
AAGATTGAAGCAGTAAAAGCTGGATACCCTGAGGCTTTGATTTTAAATCAAGAGGGTTATGTGGCAGAGTGCACAGGCGA
CAATATTTTTATAATCTCCAACGGGGTTTTGTATTCTCCACCTTCAGCCGTTGGGGCATTAGGAGGAATTACGAGAGCTA
CAGTGATTGACATAGCCAGAGAGCTTAACATTCCTTTCGAAGAAAAATACTTTTCTCTGTTTAATGTGTACACAGCTGAT
GAATGCTTCCTTACAGGTACAGCAGCAGAAGCAATTCCTGTGGTAGAAGTGGACCACAGAGTTATAGGAGACGGAAAACC
AGGACCTATAACCAAGAAAATAATAGAGGAATTTAGAAAGCTTACAAAAGTTTTGGGCACAAAGGTGTATGAATAA

Upstream 100 bases:

>100_bases
CCCCCAAATGGGGGGATTTTTTATTTTACATTGACTAATGCCTTTTAAAGTGATAAAATTTTGCTAAATTAATTTAAAAG
AAAAAGGAGGAGTCGGAGGT

Downstream 100 bases:

>100_bases
GATTGCGCATCCTGTGCAATCTTATTCATACAGCGAAAGGGTGAAGAGGATGGATAAGCTTTTTTATGAGGATAGCTATG
TTAAAGAATTTGAAGCTGTC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MGLVYVNGEFVDSEKACVSVFDHGYLYGDGIFEGIRVYDGVIFKLDEHLKRLYSMAKVLLLDIPLSMEEMKEKVVETVRI
NNLRDAYIRLVVSRGKGDLGLDPYKCPKPTVVIIADKIALYPESMYETGLKIITSSFRRNSIQSLDPQIKSLNYLNNILA
KIEAVKAGYPEALILNQEGYVAECTGDNIFIISNGVLYSPPSAVGALGGITRATVIDIARELNIPFEEKYFSLFNVYTAD
ECFLTGTAAEAIPVVEVDHRVIGDGKPGPITKKIIEEFRKLTKVLGTKVYE

Sequences:

>Translated_291_residues
MGLVYVNGEFVDSEKACVSVFDHGYLYGDGIFEGIRVYDGVIFKLDEHLKRLYSMAKVLLLDIPLSMEEMKEKVVETVRI
NNLRDAYIRLVVSRGKGDLGLDPYKCPKPTVVIIADKIALYPESMYETGLKIITSSFRRNSIQSLDPQIKSLNYLNNILA
KIEAVKAGYPEALILNQEGYVAECTGDNIFIISNGVLYSPPSAVGALGGITRATVIDIARELNIPFEEKYFSLFNVYTAD
ECFLTGTAAEAIPVVEVDHRVIGDGKPGPITKKIIEEFRKLTKVLGTKVYE
>Mature_290_residues
GLVYVNGEFVDSEKACVSVFDHGYLYGDGIFEGIRVYDGVIFKLDEHLKRLYSMAKVLLLDIPLSMEEMKEKVVETVRIN
NLRDAYIRLVVSRGKGDLGLDPYKCPKPTVVIIADKIALYPESMYETGLKIITSSFRRNSIQSLDPQIKSLNYLNNILAK
IEAVKAGYPEALILNQEGYVAECTGDNIFIISNGVLYSPPSAVGALGGITRATVIDIARELNIPFEEKYFSLFNVYTADE
CFLTGTAAEAIPVVEVDHRVIGDGKPGPITKKIIEEFRKLTKVLGTKVYE

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=282, Percent_Identity=37.9432624113475, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1787338, Length=256, Percent_Identity=25.78125, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 32347; Mature: 32216

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLVYVNGEFVDSEKACVSVFDHGYLYGDGIFEGIRVYDGVIFKLDEHLKRLYSMAKVLL
CEEEEECCEEECCHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDIPLSMEEMKEKVVETVRINNLRDAYIRLVVSRGKGDLGLDPYKCPKPTVVIIADKIAL
HCCCCCHHHHHHHHHHHHHHCCHHHHHHEEEEECCCCCCCCCCCCCCCCEEEEEECCHHH
YPESMYETGLKIITSSFRRNSIQSLDPQIKSLNYLNNILAKIEAVKAGYPEALILNQEGY
CCHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
VAECTGDNIFIISNGVLYSPPSAVGALGGITRATVIDIARELNIPFEEKYFSLFNVYTAD
EEEECCCEEEEEECCEEECCCHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHEECC
ECFLTGTAAEAIPVVEVDHRVIGDGKPGPITKKIIEEFRKLTKVLGTKVYE
CEEEECCHHHCEEEEEECCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GLVYVNGEFVDSEKACVSVFDHGYLYGDGIFEGIRVYDGVIFKLDEHLKRLYSMAKVLL
EEEEECCEEECCHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDIPLSMEEMKEKVVETVRINNLRDAYIRLVVSRGKGDLGLDPYKCPKPTVVIIADKIAL
HCCCCCHHHHHHHHHHHHHHCCHHHHHHEEEEECCCCCCCCCCCCCCCCEEEEEECCHHH
YPESMYETGLKIITSSFRRNSIQSLDPQIKSLNYLNNILAKIEAVKAGYPEALILNQEGY
CCHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
VAECTGDNIFIISNGVLYSPPSAVGALGGITRATVIDIARELNIPFEEKYFSLFNVYTAD
EEEECCCEEEEEECCEEECCCHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHEECC
ECFLTGTAAEAIPVVEVDHRVIGDGKPGPITKKIIEEFRKLTKVLGTKVYE
CEEEECCHHHCEEEEEECCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]