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The map label for this gene is CafA [H]

Identifier: 20807392

GI number: 20807392

Start: 919149

End: 920567

Strand: Direct

Name: CafA [H]

Synonym: TTE0911

Alternate gene names: 20807392

Gene position: 919149-920567 (Clockwise)

Preceding gene: 20807391

Following gene: 20807393

Centisome position: 34.18

GC content: 36.15

Gene sequence:

>1419_bases
GTGAAAATTTTGAAAAGGCTTATTATTGATATAGATGAAAAACTGTGTCAGATTGCTTTTTTAGAAGATGGGAAACTAAA
AGAGTACAGGCCAGAGAGAAAAAACGGCAAAAATATCTTGGGGAATATTTACAAGGGGAAAGTAGAAAACGTCTTAAAGG
GGATGCAGGCAGCCTTTGTAGATATAGGTCTTAATAAAAATGCTTTTTTATTTTTAGAAGATGTTGTGGGGAGGGAAGAC
AAGTCCATAACACAAGTTTTAAAACCGGGTCAGGAAGTGATGGTGCAGGTTACAAAAGAGGCAATTGGCCTTAAAAGTCC
TCGTGTTACAACTAATATTTCAATACCGGGCGAATATGTGGTTTTGATGCCTTTTATGAACTATGTGAACGTCTCTCACA
GGATTGAAAATCCGGCAGATAGGGCAAAGTTGGCCGAGATTGCGAGAAGACTAAAGCCTGAAGGCATGGGACTCATAATG
AGGACATCTTCTAAAAATGCCAAAGAGGAAGAGATAAAAGAGGATATAGAGAAATTACTTTCTGTTTACGAGAAAATAAA
AGAAAATTTTAAGTTGCTTCCTTCTCCTTCCCTCATTTATAGCGAGGAAAGCATTGCTGTGAAGTATTTGAGGGACTTTC
TTTCTTATGATGTGGACGAAATTGTGATAAACGATGAAGAAGAATATGAGAAAGTGAATCAGTACTTAAAAAAGATTGGA
AAAGATATTGAAATAGTTCTTGAAAGAGGGGATGTCTCAGGAATATATGGAGTGGACTTTCAAGTAGAAAAGCTTCTAAA
CAAAAGGGTTTGGCTCAAAAGCGGAGGTTTTTTGGTTATAGACCAGACAGAGGCTTTAACTGTTATTGATGTCAATACGG
GGAAATATGTTGGTAAGAGTTCGCTTGAAGAGACCATATTTAAGACGAATTTGGAAGCGGCGGAAGAGATTGCTTTGCAG
CTTAAATTAAGAGATATTGGAGGGATTATACTCATAGACTTTATAGATATGAAAAATGAAAATTTTAAAAAGGAATTGCT
GGAATTTTTTAAAGAGAAATTGAAACAGGATAGGGCAAAGTGCACGGTTTTAGGATTTACACAGCTTGGGCTTGTAGAGA
TGACGCGGAAAAAAGTCAGAGAAGAGATTTCCAGCTTTTTAAAAGTTAAATGTCCGGTGTGTGGTGGTACTGGTACAGTG
TATTCTGAGGAGTACATATACAAAAGGCTTAAAAACCAGATTGAGAGAATATTAAGACATGTTTTGGTGAAAAAAGTGAT
AGTAAAAGGGGATAAAAAAGTGGGAGAAATTCTTAGCAGAAAAAAGCTTATAGAGGAATACGAAAAAAGTCGGGGAATTA
AACTCATCTTTGAAGAGGTTTTTAGCGAATACGGTAAATTTGAAGTGGAATTTGAATGA

Upstream 100 bases:

>100_bases
TCACCTTTTAAAAGCTTTTAATGAATATATAGGGGATGCTTTTGATTTGGAGTATTATAAAATTCACAGAAAGAACATGT
TTTTAGAGTGAGGAACTGAG

Downstream 100 bases:

>100_bases
ATTGTGTTGACAACTTAAAAGGATAAACTTATTATAAAGATAAGAAATTGTGTTAATGATTTGAAAAAGAGGTGGATTTT
TATGAAAGGGCAGTATTACA

Product: ribonuclease G and E

Products: NA

Alternate protein names: RNase G; Cytoplasmic axial filament protein [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MKILKRLIIDIDEKLCQIAFLEDGKLKEYRPERKNGKNILGNIYKGKVENVLKGMQAAFVDIGLNKNAFLFLEDVVGRED
KSITQVLKPGQEVMVQVTKEAIGLKSPRVTTNISIPGEYVVLMPFMNYVNVSHRIENPADRAKLAEIARRLKPEGMGLIM
RTSSKNAKEEEIKEDIEKLLSVYEKIKENFKLLPSPSLIYSEESIAVKYLRDFLSYDVDEIVINDEEEYEKVNQYLKKIG
KDIEIVLERGDVSGIYGVDFQVEKLLNKRVWLKSGGFLVIDQTEALTVIDVNTGKYVGKSSLEETIFKTNLEAAEEIALQ
LKLRDIGGIILIDFIDMKNENFKKELLEFFKEKLKQDRAKCTVLGFTQLGLVEMTRKKVREEISSFLKVKCPVCGGTGTV
YSEEYIYKRLKNQIERILRHVLVKKVIVKGDKKVGEILSRKKLIEEYEKSRGIKLIFEEVFSEYGKFEVEFE

Sequences:

>Translated_472_residues
MKILKRLIIDIDEKLCQIAFLEDGKLKEYRPERKNGKNILGNIYKGKVENVLKGMQAAFVDIGLNKNAFLFLEDVVGRED
KSITQVLKPGQEVMVQVTKEAIGLKSPRVTTNISIPGEYVVLMPFMNYVNVSHRIENPADRAKLAEIARRLKPEGMGLIM
RTSSKNAKEEEIKEDIEKLLSVYEKIKENFKLLPSPSLIYSEESIAVKYLRDFLSYDVDEIVINDEEEYEKVNQYLKKIG
KDIEIVLERGDVSGIYGVDFQVEKLLNKRVWLKSGGFLVIDQTEALTVIDVNTGKYVGKSSLEETIFKTNLEAAEEIALQ
LKLRDIGGIILIDFIDMKNENFKKELLEFFKEKLKQDRAKCTVLGFTQLGLVEMTRKKVREEISSFLKVKCPVCGGTGTV
YSEEYIYKRLKNQIERILRHVLVKKVIVKGDKKVGEILSRKKLIEEYEKSRGIKLIFEEVFSEYGKFEVEFE
>Mature_472_residues
MKILKRLIIDIDEKLCQIAFLEDGKLKEYRPERKNGKNILGNIYKGKVENVLKGMQAAFVDIGLNKNAFLFLEDVVGRED
KSITQVLKPGQEVMVQVTKEAIGLKSPRVTTNISIPGEYVVLMPFMNYVNVSHRIENPADRAKLAEIARRLKPEGMGLIM
RTSSKNAKEEEIKEDIEKLLSVYEKIKENFKLLPSPSLIYSEESIAVKYLRDFLSYDVDEIVINDEEEYEKVNQYLKKIG
KDIEIVLERGDVSGIYGVDFQVEKLLNKRVWLKSGGFLVIDQTEALTVIDVNTGKYVGKSSLEETIFKTNLEAAEEIALQ
LKLRDIGGIILIDFIDMKNENFKKELLEFFKEKLKQDRAKCTVLGFTQLGLVEMTRKKVREEISSFLKVKCPVCGGTGTV
YSEEYIYKRLKNQIERILRHVLVKKVIVKGDKKVGEILSRKKLIEEYEKSRGIKLIFEEVFSEYGKFEVEFE

Specific function: Involved in the processing of the 5'-end of 16S rRNA. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles or merely regulate the formation of this structure [H]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm, cytoskeleton [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082235, Length=448, Percent_Identity=38.8392857142857, Blast_Score=307, Evalue=1e-84,
Organism=Escherichia coli, GI1787325, Length=422, Percent_Identity=36.4928909952607, Blast_Score=259, Evalue=4e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: 3.1.4.- [C]

Molecular weight: Translated: 54268; Mature: 54268

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILKRLIIDIDEKLCQIAFLEDGKLKEYRPERKNGKNILGNIYKGKVENVLKGMQAAFV
CHHHHHHHHHHHHHHHEEHCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DIGLNKNAFLFLEDVVGREDKSITQVLKPGQEVMVQVTKEAIGLKSPRVTTNISIPGEYV
EECCCCCEEEEEHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEE
VLMPFMNYVNVSHRIENPADRAKLAEIARRLKPEGMGLIMRTSSKNAKEEEIKEDIEKLL
EEEHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHH
SVYEKIKENFKLLPSPSLIYSEESIAVKYLRDFLSYDVDEIVINDEEEYEKVNQYLKKIG
HHHHHHHHCCEECCCCCEEECCHHHHHHHHHHHHCCCCHHEEECCHHHHHHHHHHHHHHC
KDIEIVLERGDVSGIYGVDFQVEKLLNKRVWLKSGGFLVIDQTEALTVIDVNTGKYVGKS
CCEEEEEECCCCCCEECCCHHHHHHHHHHEEEECCCEEEEECCCEEEEEECCCCCCCCHH
SLEETIFKTNLEAAEEIALQLKLRDIGGIILIDFIDMKNENFKKELLEFFKEKLKQDRAK
HHHHHHHHHCHHHHHHHHHHEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHC
CTVLGFTQLGLVEMTRKKVREEISSFLKVKCPVCGGTGTVYSEEYIYKRLKNQIERILRH
EEEEEHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VLVKKVIVKGDKKVGEILSRKKLIEEYEKSRGIKLIFEEVFSEYGKFEVEFE
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MKILKRLIIDIDEKLCQIAFLEDGKLKEYRPERKNGKNILGNIYKGKVENVLKGMQAAFV
CHHHHHHHHHHHHHHHEEHCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DIGLNKNAFLFLEDVVGREDKSITQVLKPGQEVMVQVTKEAIGLKSPRVTTNISIPGEYV
EECCCCCEEEEEHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEE
VLMPFMNYVNVSHRIENPADRAKLAEIARRLKPEGMGLIMRTSSKNAKEEEIKEDIEKLL
EEEHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHH
SVYEKIKENFKLLPSPSLIYSEESIAVKYLRDFLSYDVDEIVINDEEEYEKVNQYLKKIG
HHHHHHHHCCEECCCCCEEECCHHHHHHHHHHHHCCCCHHEEECCHHHHHHHHHHHHHHC
KDIEIVLERGDVSGIYGVDFQVEKLLNKRVWLKSGGFLVIDQTEALTVIDVNTGKYVGKS
CCEEEEEECCCCCCEECCCHHHHHHHHHHEEEECCCEEEEECCCEEEEEECCCCCCCCHH
SLEETIFKTNLEAAEEIALQLKLRDIGGIILIDFIDMKNENFKKELLEFFKEKLKQDRAK
HHHHHHHHHCHHHHHHHHHHEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHC
CTVLGFTQLGLVEMTRKKVREEISSFLKVKCPVCGGTGTVYSEEYIYKRLKNQIERILRH
EEEEEHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VLVKKVIVKGDKKVGEILSRKKLIEEYEKSRGIKLIFEEVFSEYGKFEVEFE
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]