Click here to switch to the map view.

The map label for this gene is RpoE2 [H]

Identifier: 20807353

GI number: 20807353

Start: 881244

End: 881819

Strand: Direct

Name: RpoE2 [H]

Synonym: TTE0872

Alternate gene names: 20807353

Gene position: 881244-881819 (Clockwise)

Preceding gene: 20807348

Following gene: 20807354

Centisome position: 32.77

GC content: 36.98

Gene sequence:

>576_bases
GTGGGACAGGAGGAACTTTTGGAAAGAGCAAGAAATGGAGATGATGAAGCATTTAGCTTGCTCATAGAAAACTATGAGAA
GTATGTGTATAATGTCATATTGAGAATTGTGGAAGAAAAAGAAGAAGCAAAAGATATTGCCCAGGAAACTTTTATTAAAG
CTTACATGAGCATAAAAACTTTTAGAAAAGACAGCAGTTTCAGGACCTGGATTTACAGGATTGCTGTAAATACTGCTATG
GATTACCTTAGAAAAAAGGTCAGGTCGGAAGTCAACCTTGTGACAGCCGCTGAAGATGAATTAGAAATAAAAAGTTTTCA
AACGCCTGAAGAGGTAATTGAGCAAAAGCTCACTGTAGAAATGGTGAGAAGAGAGATAAGTAAACTCCCTGTAGATTACA
AAATAGCTCTTATTTTAAGGGATATAGAAGGGATGAATTATGAAGAAATTGCTAGTATATTAAAAGTCAATCTCGGGACA
GTGAAGTCCAGGATATGGAGAGCGAGAAATCTTTTAAGAGAGAGGATAAAATCGCTTCCAGAGTTTTCAGGTTTTCAAGA
GAGGAGGCGAGAGTGA

Upstream 100 bases:

>100_bases
ACATCTATTGTACAAAAATTTTTACTCTACTGCAATGGATTATTTTAAGGAACAATTTTTAAATAATTTCGTCTAAAAAG
TAGAAGGGGAGGTCTAGAAG

Downstream 100 bases:

>100_bases
TGGATTGCAAAAGAGCCCTTGAACTTATGCAGGCATATTTTGATAATGAATTAGGGGAGAAGGAAAAAGAAAAATTGGAA
GAACATTTGAGAACTTGTGA

Product: DNA-directed RNA polymerase specialized sigma subunits, sigma24 homologs

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 191; Mature: 190

Protein sequence:

>191_residues
MGQEELLERARNGDDEAFSLLIENYEKYVYNVILRIVEEKEEAKDIAQETFIKAYMSIKTFRKDSSFRTWIYRIAVNTAM
DYLRKKVRSEVNLVTAAEDELEIKSFQTPEEVIEQKLTVEMVRREISKLPVDYKIALILRDIEGMNYEEIASILKVNLGT
VKSRIWRARNLLRERIKSLPEFSGFQERRRE

Sequences:

>Translated_191_residues
MGQEELLERARNGDDEAFSLLIENYEKYVYNVILRIVEEKEEAKDIAQETFIKAYMSIKTFRKDSSFRTWIYRIAVNTAM
DYLRKKVRSEVNLVTAAEDELEIKSFQTPEEVIEQKLTVEMVRREISKLPVDYKIALILRDIEGMNYEEIASILKVNLGT
VKSRIWRARNLLRERIKSLPEFSGFQERRRE
>Mature_190_residues
GQEELLERARNGDDEAFSLLIENYEKYVYNVILRIVEEKEEAKDIAQETFIKAYMSIKTFRKDSSFRTWIYRIAVNTAMD
YLRKKVRSEVNLVTAAEDELEIKSFQTPEEVIEQKLTVEMVRREISKLPVDYKIALILRDIEGMNYEEIASILKVNLGTV
KSRIWRARNLLRERIKSLPEFSGFQERRRE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=180, Percent_Identity=40, Blast_Score=135, Evalue=2e-33,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22678; Mature: 22547

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQEELLERARNGDDEAFSLLIENYEKYVYNVILRIVEEKEEAKDIAQETFIKAYMSIKT
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRKDSSFRTWIYRIAVNTAMDYLRKKVRSEVNLVTAAEDELEIKSFQTPEEVIEQKLTVE
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHH
MVRREISKLPVDYKIALILRDIEGMNYEEIASILKVNLGTVKSRIWRARNLLRERIKSLP
HHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EFSGFQERRRE
CCCCHHHHHCC
>Mature Secondary Structure 
GQEELLERARNGDDEAFSLLIENYEKYVYNVILRIVEEKEEAKDIAQETFIKAYMSIKT
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRKDSSFRTWIYRIAVNTAMDYLRKKVRSEVNLVTAAEDELEIKSFQTPEEVIEQKLTVE
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHH
MVRREISKLPVDYKIALILRDIEGMNYEEIASILKVNLGTVKSRIWRARNLLRERIKSLP
HHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EFSGFQERRRE
CCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]