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The map label for this gene is GltB2 [C]

Identifier: 20807301

GI number: 20807301

Start: 823459

End: 824964

Strand: Reverse

Name: GltB2 [C]

Synonym: TTE0819

Alternate gene names: 20807301

Gene position: 824964-823459 (Counterclockwise)

Preceding gene: 20807302

Following gene: 20807300

Centisome position: 30.67

GC content: 38.78

Gene sequence:

>1506_bases
ATGAGTTTAAGCTACCTTACTCCAGAATTTAAAGTACTTAGAGATTACGATAAATGTATTAACTGTAAGGTTTGTATGAG
AGAGTGTTCCTTTGAAGTTCACAGTTATGATGAACATCTAGATAAGATGGTTTCAAATGATATGAATTGTGTAAATTGTC
AAAGATGTGTCGTATTGTGTCCAACACAAGCTCTTACAATTATTAAACATCCTCAAACATTTAAAGAACATGCTAATTGG
ACTTACGATGCTATAAGAGATATATACAGACAGGCAGAAACGGGTGGAGTTCTTCTGTCATCCATGGGAAATGATAGGCC
TTATCCTGTATATTGGGATAAAATGGTTATAAATGCAAGTCAGGTTACAAACCCGTCTATAGACCCGTTAAGAGAACCTA
TAGAGTTAAGGGTATATTTAGGTAGAAAGCCTGATAGGATAGAAATAGACGAAAACCTCAATGTTAAAACAAAAATGCCT
CCTCAACTCAAACTTGAGACACCAATAATGTTTTCAGCAATGTCTTATGGTTCAATAAGTTATAATGCTCACGCAGCTTT
AGCAAGAGCTGCTGAAGAGCTTGGGATATTTTATAATAGTGGTGAAGGGGGGCTTCATAAGGACTTCCGCAAATATGGCA
AAAATACAATTGTACAGGTAGCATCAGGTCGTTTTGGTGTTGATAGAGAATACCTCAATATTGCGGCGGCTATTGAAATA
AAAATTGGTCAAGGTGCAAAGCCTGGGATAGGTGGTCATCTACCCGGTGAAAAGGTGAGTGAAGACATCTCGGCTACAAG
GATGATACCACCTGGAAGTGATGCTATATCTCCTGCACCGCATCATGATATCTATTCAATTGAAGACCTTGCACAATTAA
TATATGCATTGAAGGAAGCTACCGATTATCAAAAACCTGTTGGTGTTAAAATTGCAGCAGTCAATAATGTAGCGGCAATA
GCTTCGGGGATAGCAAGAGCTGGAGCAGATTATATAGCAATAGACGGATTTAGAGGCGGTACAGGGGCTGCACCAAAGAG
AATAAGGGATAACGTAGGAATTCCAATTGAATTTGCAATAGCTGCTGTAGATGCAAGACTTCGCTCCGAAGGTATAAGAC
ATACAATATCTTTAGTTGCAGCAGGTAGTATTAGAAACAGTGCAGACATAGTCAAAGCAATAGCTCTTGGTGCAGATGCT
GTTTACATTGGAACAGCCGCTTTGATTGCTCTGGGATGTCACATGTGCCAAAAATGCTATACTGGAAAATGCAACTGGGG
AATTGCTACACAAGATCCTAACCTTGCAAAAAGATTAAACCCAGAAATCGGATATAAACGTCTTGTTAACCTTGTACAAG
CATGGAGTCATGAAATAAAAGAAATGCTTGGCGGAATGGGTATAAATGATATTGAAAGTCTTAAAGGCAATAGGTTAATG
CTTCGTGGGATAGGAATGACACAAAAAGAACTTGATATACTTGGCATTCTTGCTGCCGGCGAATAG

Upstream 100 bases:

>100_bases
ATCCTGAAAGCGTATGGATGCCTAGAGGTGGAGAACCTGTTATAGCTGAGCTCATACGTGAGAATGAAAAAGAAATGTAT
TCTACAAAGGAGGTTACTGC

Downstream 100 bases:

>100_bases
GAGGTGGTATAGTGAAAACCATAAATGCGTCAGGAATTCACTATAGAGCCCTTAATGAAATAATTAAAGAACTAGTTCGT
TCAGGTGAAGACATAATTAA

Product: glutamate synthase domain-containing 2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MSLSYLTPEFKVLRDYDKCINCKVCMRECSFEVHSYDEHLDKMVSNDMNCVNCQRCVVLCPTQALTIIKHPQTFKEHANW
TYDAIRDIYRQAETGGVLLSSMGNDRPYPVYWDKMVINASQVTNPSIDPLREPIELRVYLGRKPDRIEIDENLNVKTKMP
PQLKLETPIMFSAMSYGSISYNAHAALARAAEELGIFYNSGEGGLHKDFRKYGKNTIVQVASGRFGVDREYLNIAAAIEI
KIGQGAKPGIGGHLPGEKVSEDISATRMIPPGSDAISPAPHHDIYSIEDLAQLIYALKEATDYQKPVGVKIAAVNNVAAI
ASGIARAGADYIAIDGFRGGTGAAPKRIRDNVGIPIEFAIAAVDARLRSEGIRHTISLVAAGSIRNSADIVKAIALGADA
VYIGTAALIALGCHMCQKCYTGKCNWGIATQDPNLAKRLNPEIGYKRLVNLVQAWSHEIKEMLGGMGINDIESLKGNRLM
LRGIGMTQKELDILGILAAGE

Sequences:

>Translated_501_residues
MSLSYLTPEFKVLRDYDKCINCKVCMRECSFEVHSYDEHLDKMVSNDMNCVNCQRCVVLCPTQALTIIKHPQTFKEHANW
TYDAIRDIYRQAETGGVLLSSMGNDRPYPVYWDKMVINASQVTNPSIDPLREPIELRVYLGRKPDRIEIDENLNVKTKMP
PQLKLETPIMFSAMSYGSISYNAHAALARAAEELGIFYNSGEGGLHKDFRKYGKNTIVQVASGRFGVDREYLNIAAAIEI
KIGQGAKPGIGGHLPGEKVSEDISATRMIPPGSDAISPAPHHDIYSIEDLAQLIYALKEATDYQKPVGVKIAAVNNVAAI
ASGIARAGADYIAIDGFRGGTGAAPKRIRDNVGIPIEFAIAAVDARLRSEGIRHTISLVAAGSIRNSADIVKAIALGADA
VYIGTAALIALGCHMCQKCYTGKCNWGIATQDPNLAKRLNPEIGYKRLVNLVQAWSHEIKEMLGGMGINDIESLKGNRLM
LRGIGMTQKELDILGILAAGE
>Mature_500_residues
SLSYLTPEFKVLRDYDKCINCKVCMRECSFEVHSYDEHLDKMVSNDMNCVNCQRCVVLCPTQALTIIKHPQTFKEHANWT
YDAIRDIYRQAETGGVLLSSMGNDRPYPVYWDKMVINASQVTNPSIDPLREPIELRVYLGRKPDRIEIDENLNVKTKMPP
QLKLETPIMFSAMSYGSISYNAHAALARAAEELGIFYNSGEGGLHKDFRKYGKNTIVQVASGRFGVDREYLNIAAAIEIK
IGQGAKPGIGGHLPGEKVSEDISATRMIPPGSDAISPAPHHDIYSIEDLAQLIYALKEATDYQKPVGVKIAAVNNVAAIA
SGIARAGADYIAIDGFRGGTGAAPKRIRDNVGIPIEFAIAAVDARLRSEGIRHTISLVAAGSIRNSADIVKAIALGADAV
YIGTAALIALGCHMCQKCYTGKCNWGIATQDPNLAKRLNPEIGYKRLVNLVQAWSHEIKEMLGGMGINDIESLKGNRLML
RGIGMTQKELDILGILAAGE

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0069

COG function: function code E; Glutamate synthase domain 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI308199519, Length=336, Percent_Identity=40.4761904761905, Blast_Score=216, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI17570289, Length=334, Percent_Identity=40.4191616766467, Blast_Score=225, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6320030, Length=333, Percent_Identity=39.9399399399399, Blast_Score=221, Evalue=2e-58,
Organism=Drosophila melanogaster, GI28574881, Length=355, Percent_Identity=40.2816901408451, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24665539, Length=355, Percent_Identity=40.2816901408451, Blast_Score=220, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013785
- InterPro:   IPR002932 [H]

Pfam domain/function: PF01645 Glu_synthase [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 54753; Mature: 54622

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSYLTPEFKVLRDYDKCINCKVCMRECSFEVHSYDEHLDKMVSNDMNCVNCQRCVVLC
CCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCEECCCEEEEEC
PTQALTIIKHPQTFKEHANWTYDAIRDIYRQAETGGVLLSSMGNDRPYPVYWDKMVINAS
CCHHEEEEECCHHHHHHCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCEEECCEEEEHH
QVTNPSIDPLREPIELRVYLGRKPDRIEIDENLNVKTKMPPQLKLETPIMFSAMSYGSIS
HCCCCCHHHHCCCEEEEEEECCCCCEEEECCCCCEEECCCCCEEECCCEEEEEECCCCCC
YNAHAALARAAEELGIFYNSGEGGLHKDFRKYGKNTIVQVASGRFGVDREYLNIAAAIEI
CCHHHHHHHHHHHHCEEEECCCCCHHHHHHHHCCCEEEEEECCCCCCCHHHEEEEEEEEE
KIGQGAKPGIGGHLPGEKVSEDISATRMIPPGSDAISPAPHHDIYSIEDLAQLIYALKEA
EECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
TDYQKPVGVKIAAVNNVAAIASGIARAGADYIAIDGFRGGTGAAPKRIRDNVGIPIEFAI
HCCCCCCCEEEEEECCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHCCCCCCHHHHH
AAVDARLRSEGIRHTISLVAAGSIRNSADIVKAIALGADAVYIGTAALIALGCHMCQKCY
HHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
TGKCNWGIATQDPNLAKRLNPEIGYKRLVNLVQAWSHEIKEMLGGMGINDIESLKGNRLM
CCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEE
LRGIGMTQKELDILGILAAGE
EEECCCCHHHCCEEEEEECCC
>Mature Secondary Structure 
SLSYLTPEFKVLRDYDKCINCKVCMRECSFEVHSYDEHLDKMVSNDMNCVNCQRCVVLC
CCCCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCEECCCEEEEEC
PTQALTIIKHPQTFKEHANWTYDAIRDIYRQAETGGVLLSSMGNDRPYPVYWDKMVINAS
CCHHEEEEECCHHHHHHCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCEEECCEEEEHH
QVTNPSIDPLREPIELRVYLGRKPDRIEIDENLNVKTKMPPQLKLETPIMFSAMSYGSIS
HCCCCCHHHHCCCEEEEEEECCCCCEEEECCCCCEEECCCCCEEECCCEEEEEECCCCCC
YNAHAALARAAEELGIFYNSGEGGLHKDFRKYGKNTIVQVASGRFGVDREYLNIAAAIEI
CCHHHHHHHHHHHHCEEEECCCCCHHHHHHHHCCCEEEEEECCCCCCCHHHEEEEEEEEE
KIGQGAKPGIGGHLPGEKVSEDISATRMIPPGSDAISPAPHHDIYSIEDLAQLIYALKEA
EECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
TDYQKPVGVKIAAVNNVAAIASGIARAGADYIAIDGFRGGTGAAPKRIRDNVGIPIEFAI
HCCCCCCCEEEEEECCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHCCCCCCHHHHH
AAVDARLRSEGIRHTISLVAAGSIRNSADIVKAIALGADAVYIGTAALIALGCHMCQKCY
HHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
TGKCNWGIATQDPNLAKRLNPEIGYKRLVNLVQAWSHEIKEMLGGMGINDIESLKGNRLM
CCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEE
LRGIGMTQKELDILGILAAGE
EEECCCCHHHCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]