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The map label for this gene is AsnB [H]

Identifier: 20807299

GI number: 20807299

Start: 820533

End: 822374

Strand: Reverse

Name: AsnB [H]

Synonym: TTE0817

Alternate gene names: 20807299

Gene position: 822374-820533 (Counterclockwise)

Preceding gene: 20807300

Following gene: 20807277

Centisome position: 30.58

GC content: 35.34

Gene sequence:

>1842_bases
ATGTGTGGAATAGCGGGCTGGGTTAACTTTAATATGGACTTATCACTCCAAAATGAAGTAATCGAAAAAATGACAGACAC
TCTTAAAAAAAGAGGACCTGACGGTTCTGGGGTATGGCTATCAAAGCATTGTGTTTTGGGTCACCGCCGTTTAATTGTTA
TAGATCCACAAGGCGGAATTCAACCAATGATAAAAAAATACGGTGAAAAAAATTTTGTCATCATCCACAATGGAGAACTC
TACAATATGGATGAATTAAAAAACGAACTCATATCCTTGGGATATACTTTTAATACCCGTTCAGATACTGAAATTATCTT
GACATCTTATATAGAATGGGGTCCTTTATGTGTTAAAAAATTTAATGGAATATTTGCTTTTGCAATATGGGATGAAGTAC
AAAATAGGCTTTTTATTGCCAGGGACCATCTCGGAATAAAGCCACTATTTTATACCATTAAAAACGGATCTTTGATATTT
GGTTCAGAGATCAAAGCAATATTAGCACATCCTCATGTAAAACCAGAGATAGACTCACAAGGTCTTGCAGAAATATTTGT
AATGGGACCTTCAAGAACTCCCGGATGTGGTGTCTTTAAAGACATTAAAGAATTAAAAGCCGGACATTACCTCATATTTA
CCAAAAATGGAGTAAACATAAGCAAATACTGGTCATTACAAAGTCTTCCCCATGAAGATAGCCTTGAGAAAACGGCGGAA
AAAATAAGGTATCTCCTTAAAGACTCATCAGTAAGACAACTTGTTTCAGACGTACCTTTATGTTCATTGCTCTCAGGTGG
ACTTGATTCAACTGCTGTATCTTTCTTCGCAAATGAAGAATTAAAACAAAAAGGTCATAAACTTATAACATATTCTGTAG
ATTATGTAGGTAATGATAAATATTTTACGCCAAACGAATTTCAACCAAACTCTGACGCAGACTGGGTAAAATTTGTTGCA
AATAAACTTGACACAGACCATAACTATGTATTTATAGACAATGAAGAACTCGCACATGCTCTAAAGCCAGCCTTATACGC
TCGAGACCTTCCTGGTATGGCAGATATAGACTCATCTTTATATCTATTTTTAAAAGAAGTAAAAAAAGGAGCAACAGTCG
CCCTATCAGGAGAAGGTGCTGATGAAGTTTTTGGAGGTTATCCCTGGTTTAGAAATAAGGAATCAATCAGAAGTCACACA
TTCCCATGGACAAGAATGGTTAATGAACGCATGAAATTGCTTCTGCCAGAAGTTGTTAATTACATAAGGCCTTTAGAATA
TCTAAATGATAGATATAACGAAGCTTTAAATGAAGTGCCAAAGCTTAATGGAGAAGACCCCTATAATGCTCGAATGAGGG
AAATGTTTTACCTTAACATAACAAGATTTATGCCTATGCTTCTTGACAGAATGGATAGAATGAGTATGGCAGTAGGCTTA
GAAGCAAGAGTACCTTTTTTAGATTACAGACTTGTAGAATATGTATGGAACATACCGTGGGAAATGAAAAATTTAAACAA
CAGAGAAAAAGGAATACTTAGGTATGCTCTAAAAGGTTACATCCCAGACGAAGTAATTGAAAGAAAAAAGAGTCCCTACC
CTAAAACACACAATCCTGTATTTAAAAACATCGTTAAAAAATGGCTAAAACAAATAATTAACGACAGTACATCCCCACTT
TTGCAACTTGTAGATAAAAAAGCAATTGCAGAACTTATAGAAACAGATGCAAAAGCCTATGACCCTGCCTGGTTTTCACA
GCTTATGGGTTCCCCACAGCTTTTCGCTTACCTCGTACAAATAAATATGTGGTTGAAAGATTATAAAATTTCTATAGTGT
AA

Upstream 100 bases:

>100_bases
TTGTTTAAGCATGGGTGGTTCGTTAGAAAATTATAACATGTATCAATGGCAAAAAAGGAGAATATATTTAGTTATAGATT
CAAATTTTAGGAGGATTTTT

Downstream 100 bases:

>100_bases
ATAATATACTAAACAGAAGGATTTTTCTACCGTTTAAACTGATAACAACCCCACTTTTATATATAGAAAACGTGGGGGTT
GACCTTATATTTGGATGTAT

Product: asparagine synthase (glutamine-hydrolyzing)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 613; Mature: 613

Protein sequence:

>613_residues
MCGIAGWVNFNMDLSLQNEVIEKMTDTLKKRGPDGSGVWLSKHCVLGHRRLIVIDPQGGIQPMIKKYGEKNFVIIHNGEL
YNMDELKNELISLGYTFNTRSDTEIILTSYIEWGPLCVKKFNGIFAFAIWDEVQNRLFIARDHLGIKPLFYTIKNGSLIF
GSEIKAILAHPHVKPEIDSQGLAEIFVMGPSRTPGCGVFKDIKELKAGHYLIFTKNGVNISKYWSLQSLPHEDSLEKTAE
KIRYLLKDSSVRQLVSDVPLCSLLSGGLDSTAVSFFANEELKQKGHKLITYSVDYVGNDKYFTPNEFQPNSDADWVKFVA
NKLDTDHNYVFIDNEELAHALKPALYARDLPGMADIDSSLYLFLKEVKKGATVALSGEGADEVFGGYPWFRNKESIRSHT
FPWTRMVNERMKLLLPEVVNYIRPLEYLNDRYNEALNEVPKLNGEDPYNARMREMFYLNITRFMPMLLDRMDRMSMAVGL
EARVPFLDYRLVEYVWNIPWEMKNLNNREKGILRYALKGYIPDEVIERKKSPYPKTHNPVFKNIVKKWLKQIINDSTSPL
LQLVDKKAIAELIETDAKAYDPAWFSQLMGSPQLFAYLVQINMWLKDYKISIV

Sequences:

>Translated_613_residues
MCGIAGWVNFNMDLSLQNEVIEKMTDTLKKRGPDGSGVWLSKHCVLGHRRLIVIDPQGGIQPMIKKYGEKNFVIIHNGEL
YNMDELKNELISLGYTFNTRSDTEIILTSYIEWGPLCVKKFNGIFAFAIWDEVQNRLFIARDHLGIKPLFYTIKNGSLIF
GSEIKAILAHPHVKPEIDSQGLAEIFVMGPSRTPGCGVFKDIKELKAGHYLIFTKNGVNISKYWSLQSLPHEDSLEKTAE
KIRYLLKDSSVRQLVSDVPLCSLLSGGLDSTAVSFFANEELKQKGHKLITYSVDYVGNDKYFTPNEFQPNSDADWVKFVA
NKLDTDHNYVFIDNEELAHALKPALYARDLPGMADIDSSLYLFLKEVKKGATVALSGEGADEVFGGYPWFRNKESIRSHT
FPWTRMVNERMKLLLPEVVNYIRPLEYLNDRYNEALNEVPKLNGEDPYNARMREMFYLNITRFMPMLLDRMDRMSMAVGL
EARVPFLDYRLVEYVWNIPWEMKNLNNREKGILRYALKGYIPDEVIERKKSPYPKTHNPVFKNIVKKWLKQIINDSTSPL
LQLVDKKAIAELIETDAKAYDPAWFSQLMGSPQLFAYLVQINMWLKDYKISIV
>Mature_613_residues
MCGIAGWVNFNMDLSLQNEVIEKMTDTLKKRGPDGSGVWLSKHCVLGHRRLIVIDPQGGIQPMIKKYGEKNFVIIHNGEL
YNMDELKNELISLGYTFNTRSDTEIILTSYIEWGPLCVKKFNGIFAFAIWDEVQNRLFIARDHLGIKPLFYTIKNGSLIF
GSEIKAILAHPHVKPEIDSQGLAEIFVMGPSRTPGCGVFKDIKELKAGHYLIFTKNGVNISKYWSLQSLPHEDSLEKTAE
KIRYLLKDSSVRQLVSDVPLCSLLSGGLDSTAVSFFANEELKQKGHKLITYSVDYVGNDKYFTPNEFQPNSDADWVKFVA
NKLDTDHNYVFIDNEELAHALKPALYARDLPGMADIDSSLYLFLKEVKKGATVALSGEGADEVFGGYPWFRNKESIRSHT
FPWTRMVNERMKLLLPEVVNYIRPLEYLNDRYNEALNEVPKLNGEDPYNARMREMFYLNITRFMPMLLDRMDRMSMAVGL
EARVPFLDYRLVEYVWNIPWEMKNLNNREKGILRYALKGYIPDEVIERKKSPYPKTHNPVFKNIVKKWLKQIINDSTSPL
LQLVDKKAIAELIETDAKAYDPAWFSQLMGSPQLFAYLVQINMWLKDYKISIV

Specific function: Asparagine synthetase involved in sporulation [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=414, Percent_Identity=27.536231884058, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI168229252, Length=414, Percent_Identity=27.536231884058, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI168229250, Length=414, Percent_Identity=27.536231884058, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI296010848, Length=387, Percent_Identity=27.1317829457364, Blast_Score=120, Evalue=5e-27,
Organism=Homo sapiens, GI296010852, Length=329, Percent_Identity=26.4437689969605, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI296010850, Length=329, Percent_Identity=26.4437689969605, Blast_Score=91, Evalue=4e-18,
Organism=Escherichia coli, GI1786889, Length=574, Percent_Identity=27.7003484320557, Blast_Score=181, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI71993933, Length=554, Percent_Identity=26.7148014440433, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI25147557, Length=554, Percent_Identity=26.7148014440433, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17560178, Length=551, Percent_Identity=26.497277676951, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI25147560, Length=278, Percent_Identity=29.136690647482, Blast_Score=92, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6325403, Length=593, Percent_Identity=24.283305227656, Blast_Score=131, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6321563, Length=574, Percent_Identity=24.390243902439, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI45553209, Length=537, Percent_Identity=25.6983240223464, Blast_Score=149, Evalue=7e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 70495; Mature: 70495

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGWVNFNMDLSLQNEVIEKMTDTLKKRGPDGSGVWLSKHCVLGHRRLIVIDPQGGI
CCCCCEEEEEEEEECHHHHHHHHHHHHHHHCCCCCCCEEEECCEEECCCEEEEECCCCCH
QPMIKKYGEKNFVIIHNGELYNMDELKNELISLGYTFNTRSDTEIILTSYIEWGPLCVKK
HHHHHHCCCCCEEEEECCCEECHHHHHHHHHHHCEEECCCCCCEEEEEHHHCCCHHHHHH
FNGIFAFAIWDEVQNRLFIARDHLGIKPLFYTIKNGSLIFGSEIKAILAHPHVKPEIDSQ
CCCEEEEEEHHHHHCCEEEEECCCCCCEEEEEEECCCEEECCHHHHHHHCCCCCCCCCCC
GLAEIFVMGPSRTPGCGVFKDIKELKAGHYLIFTKNGVNISKYWSLQSLPHEDSLEKTAE
CCEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCCHHHEECCCCCCCCCHHHHHHH
KIRYLLKDSSVRQLVSDVPLCSLLSGGLDSTAVSFFANEELKQKGHKLITYSVDYVGNDK
HHHHHHCCHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEECCCC
YFTPNEFQPNSDADWVKFVANKLDTDHNYVFIDNEELAHALKPALYARDLPGMADIDSSL
CCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCHHH
YLFLKEVKKGATVALSGEGADEVFGGYPWFRNKESIRSHTFPWTRMVNERMKLLLPEVVN
HHHHHHHCCCCEEEECCCCCHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHH
YIRPLEYLNDRYNEALNEVPKLNGEDPYNARMREMFYLNITRFMPMLLDRMDRMSMAVGL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EARVPFLDYRLVEYVWNIPWEMKNLNNREKGILRYALKGYIPDEVIERKKSPYPKTHNPV
CCCCCHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCHH
FKNIVKKWLKQIINDSTSPLLQLVDKKAIAELIETDAKAYDPAWFSQLMGSPQLFAYLVQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHH
INMWLKDYKISIV
HHHHHCCEEEEEC
>Mature Secondary Structure
MCGIAGWVNFNMDLSLQNEVIEKMTDTLKKRGPDGSGVWLSKHCVLGHRRLIVIDPQGGI
CCCCCEEEEEEEEECHHHHHHHHHHHHHHHCCCCCCCEEEECCEEECCCEEEEECCCCCH
QPMIKKYGEKNFVIIHNGELYNMDELKNELISLGYTFNTRSDTEIILTSYIEWGPLCVKK
HHHHHHCCCCCEEEEECCCEECHHHHHHHHHHHCEEECCCCCCEEEEEHHHCCCHHHHHH
FNGIFAFAIWDEVQNRLFIARDHLGIKPLFYTIKNGSLIFGSEIKAILAHPHVKPEIDSQ
CCCEEEEEEHHHHHCCEEEEECCCCCCEEEEEEECCCEEECCHHHHHHHCCCCCCCCCCC
GLAEIFVMGPSRTPGCGVFKDIKELKAGHYLIFTKNGVNISKYWSLQSLPHEDSLEKTAE
CCEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCCHHHEECCCCCCCCCHHHHHHH
KIRYLLKDSSVRQLVSDVPLCSLLSGGLDSTAVSFFANEELKQKGHKLITYSVDYVGNDK
HHHHHHCCHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEECCCC
YFTPNEFQPNSDADWVKFVANKLDTDHNYVFIDNEELAHALKPALYARDLPGMADIDSSL
CCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCHHH
YLFLKEVKKGATVALSGEGADEVFGGYPWFRNKESIRSHTFPWTRMVNERMKLLLPEVVN
HHHHHHHCCCCEEEECCCCCHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHH
YIRPLEYLNDRYNEALNEVPKLNGEDPYNARMREMFYLNITRFMPMLLDRMDRMSMAVGL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EARVPFLDYRLVEYVWNIPWEMKNLNNREKGILRYALKGYIPDEVIERKKSPYPKTHNPV
CCCCCHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCHH
FKNIVKKWLKQIINDSTSPLLQLVDKKAIAELIETDAKAYDPAWFSQLMGSPQLFAYLVQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHH
INMWLKDYKISIV
HHHHHCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10498721 [H]