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The map label for this gene is RfaE [H]

Identifier: 20807252

GI number: 20807252

Start: 768312

End: 769361

Strand: Direct

Name: RfaE [H]

Synonym: TTE0768

Alternate gene names: 20807252

Gene position: 768312-769361 (Clockwise)

Preceding gene: 20807251

Following gene: 20807253

Centisome position: 28.57

GC content: 34.1

Gene sequence:

>1050_bases
ATGTATGAAGTTGTAAAAGATATCGGAGAGGAGAGAGTAACGGAACTTCTTCATCGATTAAGAAAAGGTAAAATAGCAGT
AATAGGAGATGTAGCATTAGATATATATTGGAGAGCAGATATGAGAAAAAGTGAGCTTTCTAGAGAAACTCCCCATTTTC
CTCTCCCTATAGTAGAAGAGTGGATGTCACCAGGTGCTGCAGGTAATGTTGCTACTAATATAGCGGCTTTAAAACCAGAA
AAACTTTTCTTGCTGAGTATTATTGGTAAAGATTGGAGAGGAGATTTATTTATAAAGGAAGTAGAGAAAAGAGGTATAAT
TACTGATTTTCTAATCAGAAGTGATGAAAGGGTTACAAATGCATATTGTAAACCATTAAAACGAGGATTTTCGGATGTAG
AGTATGAAGATCCTAGAATAGATTTTGAAAATTTTAGACTTATTACTCAGAAGGAAGAGGATGAATTAATAAAATTATTA
GAAAAAGTAGCTAAAGAAATAGATGTGCTTTGCGTAGTCGATCAGATGCGTTTTGGGTGTATCACTCCTAGAGTTAGAGA
AAAAATAGAAAATTTTTCAAGAGAAGGGTTAACAGTAATAGTGGACAGTAGGGAAAGAATAGGTTATTTTACTGATGTAA
TTTTAAAGCCAAATGATATAGAAATGTTTAAGGCTATGGGTATAGAAAAAGATCCCCAAAAATTATCTATCGAAGAATTG
TTGGAATTTGCAGAAAAATTTTCTATACAAAAAAGAAGTGATGTATGTGTTACATTAGGAGCTCGTGGGGCTATTTACGT
AGAAAAAGACACTAGTAAAAAATATTACGTTCCTACTGTTAACTTGACACCCCCCTTGGATGTTTGTGGTGCAGGGGACA
CTTTTTTGTCCATATTTTCACTTGTTTTAGGTACGGGTGGCAAGAAAGTTGAAGCAGTTTTCATGGGAAACTTAGCAGCT
AGTGTTGTTGTTAAAAAGATTGGTACTACTGGAGTTGCAACAGTTGAAGAAATTATGGACAATTATAAAAAACATTGGGC
AACCAGTTAG

Upstream 100 bases:

>100_bases
AAAGGATGGCACTTTTGCGGGGATCACAAAATTACTATACCAAATATATATAAGAAATTAATCGAAAAGATGCCTTATGT
AGAAAAGGGGATTGAGGACA

Downstream 100 bases:

>100_bases
CCCTTTATTTTTCATTTTGTTTCTTTTCATTCTTGTGTTAAAATAATTATACGCAGCATATGGGTATACCATAGGGGGGT
GTTAGTATAGAGTATATAAA

Product: ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MYEVVKDIGEERVTELLHRLRKGKIAVIGDVALDIYWRADMRKSELSRETPHFPLPIVEEWMSPGAAGNVATNIAALKPE
KLFLLSIIGKDWRGDLFIKEVEKRGIITDFLIRSDERVTNAYCKPLKRGFSDVEYEDPRIDFENFRLITQKEEDELIKLL
EKVAKEIDVLCVVDQMRFGCITPRVREKIENFSREGLTVIVDSRERIGYFTDVILKPNDIEMFKAMGIEKDPQKLSIEEL
LEFAEKFSIQKRSDVCVTLGARGAIYVEKDTSKKYYVPTVNLTPPLDVCGAGDTFLSIFSLVLGTGGKKVEAVFMGNLAA
SVVVKKIGTTGVATVEEIMDNYKKHWATS

Sequences:

>Translated_349_residues
MYEVVKDIGEERVTELLHRLRKGKIAVIGDVALDIYWRADMRKSELSRETPHFPLPIVEEWMSPGAAGNVATNIAALKPE
KLFLLSIIGKDWRGDLFIKEVEKRGIITDFLIRSDERVTNAYCKPLKRGFSDVEYEDPRIDFENFRLITQKEEDELIKLL
EKVAKEIDVLCVVDQMRFGCITPRVREKIENFSREGLTVIVDSRERIGYFTDVILKPNDIEMFKAMGIEKDPQKLSIEEL
LEFAEKFSIQKRSDVCVTLGARGAIYVEKDTSKKYYVPTVNLTPPLDVCGAGDTFLSIFSLVLGTGGKKVEAVFMGNLAA
SVVVKKIGTTGVATVEEIMDNYKKHWATS
>Mature_349_residues
MYEVVKDIGEERVTELLHRLRKGKIAVIGDVALDIYWRADMRKSELSRETPHFPLPIVEEWMSPGAAGNVATNIAALKPE
KLFLLSIIGKDWRGDLFIKEVEKRGIITDFLIRSDERVTNAYCKPLKRGFSDVEYEDPRIDFENFRLITQKEEDELIKLL
EKVAKEIDVLCVVDQMRFGCITPRVREKIENFSREGLTVIVDSRERIGYFTDVILKPNDIEMFKAMGIEKDPQKLSIEEL
LEFAEKFSIQKRSDVCVTLGARGAIYVEKDTSKKYYVPTVNLTPPLDVCGAGDTFLSIFSLVLGTGGKKVEAVFMGNLAA
SVVVKKIGTTGVATVEEIMDNYKKHWATS

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose [H]

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789432, Length=330, Percent_Identity=27.2727272727273, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB [H]

EC number: 2.7.-.-

Molecular weight: Translated: 39552; Mature: 39552

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEVVKDIGEERVTELLHRLRKGKIAVIGDVALDIYWRADMRKSELSRETPHFPLPIVEE
CCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEECCCCCHHHHHCCCCCCCCHHHHH
WMSPGAAGNVATNIAALKPEKLFLLSIIGKDWRGDLFIKEVEKRGIITDFLIRSDERVTN
HCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHCCCCHHHH
AYCKPLKRGFSDVEYEDPRIDFENFRLITQKEEDELIKLLEKVAKEIDVLCVVDQMRFGC
HHHHHHHCCCCCCCCCCCCCCHHHEEEEECCCHHHHHHHHHHHHHHHCEEEEEHHHHHCC
ITPRVREKIENFSREGLTVIVDSRERIGYFTDVILKPNDIEMFKAMGIEKDPQKLSIEEL
CCHHHHHHHHHHCCCCCEEEECCCHHCCHHHHEEECCCHHHHHHHCCCCCCCCCCCHHHH
LEFAEKFSIQKRSDVCVTLGARGAIYVEKDTSKKYYVPTVNLTPPLDVCGAGDTFLSIFS
HHHHHHHCCCCCCCEEEEECCCCEEEEEECCCCEEEEEEECCCCCHHCCCCCHHHHHHHH
LVLGTGGKKVEAVFMGNLAASVVVKKIGTTGVATVEEIMDNYKKHWATS
HHHCCCCCEEEEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MYEVVKDIGEERVTELLHRLRKGKIAVIGDVALDIYWRADMRKSELSRETPHFPLPIVEE
CCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEECCCCCHHHHHCCCCCCCCHHHHH
WMSPGAAGNVATNIAALKPEKLFLLSIIGKDWRGDLFIKEVEKRGIITDFLIRSDERVTN
HCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHCCCCHHHH
AYCKPLKRGFSDVEYEDPRIDFENFRLITQKEEDELIKLLEKVAKEIDVLCVVDQMRFGC
HHHHHHHCCCCCCCCCCCCCCHHHEEEEECCCHHHHHHHHHHHHHHHCEEEEEHHHHHCC
ITPRVREKIENFSREGLTVIVDSRERIGYFTDVILKPNDIEMFKAMGIEKDPQKLSIEEL
CCHHHHHHHHHHCCCCCEEEECCCHHCCHHHHEEECCCHHHHHHHCCCCCCCCCCCHHHH
LEFAEKFSIQKRSDVCVTLGARGAIYVEKDTSKKYYVPTVNLTPPLDVCGAGDTFLSIFS
HHHHHHHCCCCCCCEEEEECCCCEEEEEECCCCEEEEEEECCCCCHHCCCCCHHHHHHHH
LVLGTGGKKVEAVFMGNLAASVVVKKIGTTGVATVEEIMDNYKKHWATS
HHHCCCCCEEEEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA