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The map label for this gene is Tra8 [C]

Identifier: 20807224

GI number: 20807224

Start: 739538

End: 740581

Strand: Direct

Name: Tra8 [C]

Synonym: TTE0737

Alternate gene names: 20807224

Gene position: 739538-740581 (Clockwise)

Preceding gene: 20807222

Following gene: 20807226

Centisome position: 27.5

GC content: 31.42

Gene sequence:

>1044_bases
ATGGTTCATAATAATGATACCACAAAAAAGCGTTCTTTTAAACACTTAAGTAGCTACGAACGAGGAGAGATCTATGCATT
ACTCAAAGAAGGAAGAAGTATTCGGTATATTGCTAAAAAACTTAATCGATCTCCAAGCACTATAAGCCGTGAAATTAAAC
GTGGAACTACTACACAACTTAGAAGTGATTTATCTTCTTATACAAGCTATTTTCCTGAAACCGGTCAAGCTATCTACGAA
AAAAATCGCTCAAATTGCGGAGCTAAATTTAAAGTAGCTAAAGCAGAAGATTTCTTGAAATATGCTGAAAATAAAATATT
AAATGAAAAATGGTCACCAGATGCAGTTGTAGGTTATTGTAAAAAGGACCCAAGCTGGAATAATAAAACTATTGTTTGTA
CTAAAACACTGTACAACTATATAGATAGAGGATTATTAAAAGTTAAAAACATTGATTTACCTTTAAAACTACGCTTAAAA
CCAAGGAAGAAACAAAACCGTAAAAATAAACGTATTATGGGTAAAAGTATTGATTTTAGGCCTAAAGAAGTTGAAAGCCG
TGAAGTTTTTGGGCATTGGGAAATAGATACGTTAATTGGCAAGAAATCTAATGACAAGGTCCTTTTAACATTAATAGAGC
GTAAGACTCGCCATGAAATAATATTCTTATTAGATGCAAAAGACAATAAATCTGTTAAAGATGCATTATCAAAATTAAAA
GATATGTTTGGTGACAATTTAAGCAAGGTCTTTAAAACCATAACATCTGATAATGGTACAGAGTTTAGTGATTTAGAAAG
TGCTCTTTTAGAATATGGCGTAGAAGTATATTATACACATCCATATTCATCTTGGGAAAGAGCTACAAATGAACGACATA
ACGGTCTTATACGACGTTTCATCCCTAAAGGTAAAAGTATTAAAGATTTATCTATAGATACGATAAAGAGAGTAGAAAAC
TGGCTTAATAACCTTCCACGAAAATTGTTAAATTACAAAACGCCTAAGGAATACTTTTATGAAGAGCTGGCAAAAATCTG
TTAA

Upstream 100 bases:

>100_bases
GTTGTATAATTAAATGCAACAGATAAAAGAATAGAAACCATAGTGAAAATCGTGTATGATATAGGTGTCAACTAAACATC
ATACAAGGAGGATTTTCACT

Downstream 100 bases:

>100_bases
GCCCATCCCTTATAAAGTTTAGAGCTCGTTCGTGATTTGTCAAGGGGCAGGCTTCGACTGACTTTAACCCTTGACAAATC
ACGACCTTCGCTCAATTTGT

Product: IS30 family transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MVHNNDTTKKRSFKHLSSYERGEIYALLKEGRSIRYIAKKLNRSPSTISREIKRGTTTQLRSDLSSYTSYFPETGQAIYE
KNRSNCGAKFKVAKAEDFLKYAENKILNEKWSPDAVVGYCKKDPSWNNKTIVCTKTLYNYIDRGLLKVKNIDLPLKLRLK
PRKKQNRKNKRIMGKSIDFRPKEVESREVFGHWEIDTLIGKKSNDKVLLTLIERKTRHEIIFLLDAKDNKSVKDALSKLK
DMFGDNLSKVFKTITSDNGTEFSDLESALLEYGVEVYYTHPYSSWERATNERHNGLIRRFIPKGKSIKDLSIDTIKRVEN
WLNNLPRKLLNYKTPKEYFYEELAKIC

Sequences:

>Translated_347_residues
MVHNNDTTKKRSFKHLSSYERGEIYALLKEGRSIRYIAKKLNRSPSTISREIKRGTTTQLRSDLSSYTSYFPETGQAIYE
KNRSNCGAKFKVAKAEDFLKYAENKILNEKWSPDAVVGYCKKDPSWNNKTIVCTKTLYNYIDRGLLKVKNIDLPLKLRLK
PRKKQNRKNKRIMGKSIDFRPKEVESREVFGHWEIDTLIGKKSNDKVLLTLIERKTRHEIIFLLDAKDNKSVKDALSKLK
DMFGDNLSKVFKTITSDNGTEFSDLESALLEYGVEVYYTHPYSSWERATNERHNGLIRRFIPKGKSIKDLSIDTIKRVEN
WLNNLPRKLLNYKTPKEYFYEELAKIC
>Mature_347_residues
MVHNNDTTKKRSFKHLSSYERGEIYALLKEGRSIRYIAKKLNRSPSTISREIKRGTTTQLRSDLSSYTSYFPETGQAIYE
KNRSNCGAKFKVAKAEDFLKYAENKILNEKWSPDAVVGYCKKDPSWNNKTIVCTKTLYNYIDRGLLKVKNIDLPLKLRLK
PRKKQNRKNKRIMGKSIDFRPKEVESREVFGHWEIDTLIGKKSNDKVLLTLIERKTRHEIIFLLDAKDNKSVKDALSKLK
DMFGDNLSKVFKTITSDNGTEFSDLESALLEYGVEVYYTHPYSSWERATNERHNGLIRRFIPKGKSIKDLSIDTIKRVEN
WLNNLPRKLLNYKTPKEYFYEELAKIC

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG2826

COG function: function code L; Transposase and inactivated derivatives, IS30 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1790736, Length=348, Percent_Identity=33.9080459770115, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1787671, Length=348, Percent_Identity=33.9080459770115, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1786450, Length=348, Percent_Identity=33.9080459770115, Blast_Score=155, Evalue=5e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 40588; Mature: 40588

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVHNNDTTKKRSFKHLSSYERGEIYALLKEGRSIRYIAKKLNRSPSTISREIKRGTTTQL
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHCCHHHHH
RSDLSSYTSYFPETGQAIYEKNRSNCGAKFKVAKAEDFLKYAENKILNEKWSPDAVVGYC
HHHHHHHHHHCCCCCHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCEEEEE
KKDPSWNNKTIVCTKTLYNYIDRGLLKVKNIDLPLKLRLKPRKKQNRKNKRIMGKSIDFR
CCCCCCCCCEEEEHHHHHHHHHHHHEEEECCCCCEEEEECCCHHHCHHHHHHCCCCCCCC
PKEVESREVFGHWEIDTLIGKKSNDKVLLTLIERKTRHEIIFLLDAKDNKSVKDALSKLK
CCCCCCCHHCCCEEHHHHHCCCCCCCEEEHHHHHCCCCEEEEEEECCCCCHHHHHHHHHH
DMFGDNLSKVFKTITSDNGTEFSDLESALLEYGVEVYYTHPYSSWERATNERHNGLIRRF
HHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHH
IPKGKSIKDLSIDTIKRVENWLNNLPRKLLNYKTPKEYFYEELAKIC
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHC
>Mature Secondary Structure
MVHNNDTTKKRSFKHLSSYERGEIYALLKEGRSIRYIAKKLNRSPSTISREIKRGTTTQL
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHCCHHHHH
RSDLSSYTSYFPETGQAIYEKNRSNCGAKFKVAKAEDFLKYAENKILNEKWSPDAVVGYC
HHHHHHHHHHCCCCCHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCEEEEE
KKDPSWNNKTIVCTKTLYNYIDRGLLKVKNIDLPLKLRLKPRKKQNRKNKRIMGKSIDFR
CCCCCCCCCEEEEHHHHHHHHHHHHEEEECCCCCEEEEECCCHHHCHHHHHHCCCCCCCC
PKEVESREVFGHWEIDTLIGKKSNDKVLLTLIERKTRHEIIFLLDAKDNKSVKDALSKLK
CCCCCCCHHCCCEEHHHHHCCCCCCCEEEHHHHHCCCCEEEEEEECCCCCHHHHHHHHHH
DMFGDNLSKVFKTITSDNGTEFSDLESALLEYGVEVYYTHPYSSWERATNERHNGLIRRF
HHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHH
IPKGKSIKDLSIDTIKRVENWLNNLPRKLLNYKTPKEYFYEELAKIC
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8393068 [H]