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The map label for this gene is NapF

Identifier: 20807184

GI number: 20807184

Start: 702933

End: 704678

Strand: Direct

Name: NapF

Synonym: TTE0697

Alternate gene names: NA

Gene position: 702933-704678 (Clockwise)

Preceding gene: 20807183

Following gene: 20807185

Centisome position: 26.14

GC content: 46.68

Gene sequence:

>1746_bases
ATGGGAGGAGGATTGATTTTGGATGTAATCAGTGTATCTAAGGCAAACTGCAGAAATTGCTACCGGTGCGTTAGGCATTG
TCCTGTCAAAGCTATAAAAGTAGTCGGCGGTCAGGCGGAAGTTGTGGATAGCTTATGTATATACTGTGGAAGGTGTGTCA
TCGAATGCCCCCAGAATGCCAAGAAAGTACGGAACGATCTACATGCAGTAAAAGAATTCATATATGCCGGCGAAAAGGTT
ATAGCCTCCATAGCTCCTTCTTACCCTGCTGTCTTTGACGTAACTTCTCCTCAGGAAATGTTTTCCATACTGAAGGCCCT
AGGGTTTTATGGAGCTGAGGAAACAGCGGTGGGAGCTGAAATGGTATCTATGGAGTACCAGCATTTGATCAAAAAGGGGC
ATAAGGGTCCCATCATAACCACGGCTTGCCCGGTTGTAAAAAATCTGGTAGAGAAATATTATCCAGGACTTATAAGGAAT
CTAGCTCCGGTGGTTTCCCCGATGGTTGCTCATGCAAGGTCATTGAAGCAAAGATATGGGTTGGACATTAAAGTTGTTTT
TATAGGTCCCTGCATTGCCAAAAAAGCTGAAGCCCTAGATAGAAGCGTTTTAGGGGATGTAGATGCAGTACTAACTTTTC
AAGAATTGGCCGATTGGATAGATGAGAAAGCCTCCGCGGTAGCTGGAGTAGACTTTGGAGAGCGAGAAAATGTATTAGAA
CCTTTTATTGCCAGGAGTTATCCCTTGCCAGGGGGGCTTTTAAAGACTTCTTCAATGGAGGGTGACCTTTTGTCTCGAGA
GGTCGTAGTCGTGGACGGTATAGAAAACTGTATGGAGCTTTTAGATGCCCTGGAGATGGGAAACGTAAGTGGTCGCATAT
TTGAGATGATGGCCTGTAGGGGTGGCTGTATCAATGGGCCCTCGATGCCGTGTGACAGTTCCCTTTATGAAAGAAGGGAA
AGGTTGCTTTCATTTATAGAAAATAATCAACAGCAGCAGGGATATGAGAATATAAAGGCTTCTGCGGCGGGTATTGACCT
CAGGAGGTCTTTCGAGCCGAAGGCTTTTAAAGCGCCTAGGCCAACCGAATCAGAGATAAGAGAAATACTGGCTTCAATAG
GCAAAACGACTCCAGAAAAGGAACTGAACTGCGGGGCATGCGGATATCCTTCCTGCCGCAAAAAAGCCGAGGCTGTCTAC
CATGGAATGGCGGAGCCGGACATGTGCATCCCCTACATGAGGTCCAGGGCCGAATCTTTGGCCAACCTGATCATAGACCA
CACCCCGAACGGCATCATTTTGGTAGACAGTAATTTGAATATAAAGGAAATAAATAGAGCGGCGGAAAAAATGTTCGGTG
TTGAAAAGGAACAGGTCCAGGGGAAGCCCCTTTTCACTGTCATCGACGACAGGGATTTTGCCTGGGTACTGGCCAATAAA
ACTAACCTGCAGGATAAAAAGGTGAACTATCCCCGGAATTCTTTAACTACCCTCCAGACTATGTGCTATATCGAGGAAGA
GGACATGGTACTGGCGATTATTCAGGATATTACCGAACAGGAAAAGCAGAGGATGGAACTGGAAAGGGTTAGGGAGGAGA
CCCTGGAGAAGGCCCAGGAAGTAATAAACAGGCAGATGCGCGTCGCCCAAGAAATAGCGGGACTGCTGGGAGAGACGACG
GCGGAGAGCAAGATGCTGCTCCTTAAGCTTATAGAACTGGTTAAAGGCAGGGAAGAAGAAAAGTGA

Upstream 100 bases:

>100_bases
AGGGTGTGTCAAAGGAGTAATGATTAAGGTTGCCGATAAATATTTCACTTCTGTGACGCGCAGGAGGTGGCGAGGATTTA
GAAGCTATAAAGAATAGTAA

Downstream 100 bases:

>100_bases
AGATCTTTGCTGAAGTGTTCACCAAAAGCCTTACCAAAAAAGGAGAAGAGCTGTGCGGGGACAGCGTTGAGGTTGTGCAA
TCCGAAGACGGCATAATTGC

Product: ferredoxin 2

Products: NA

Alternate protein names: CpI; Fe-only hydrogenase; [Fe] hydrogenase [H]

Number of amino acids: Translated: 581; Mature: 580

Protein sequence:

>581_residues
MGGGLILDVISVSKANCRNCYRCVRHCPVKAIKVVGGQAEVVDSLCIYCGRCVIECPQNAKKVRNDLHAVKEFIYAGEKV
IASIAPSYPAVFDVTSPQEMFSILKALGFYGAEETAVGAEMVSMEYQHLIKKGHKGPIITTACPVVKNLVEKYYPGLIRN
LAPVVSPMVAHARSLKQRYGLDIKVVFIGPCIAKKAEALDRSVLGDVDAVLTFQELADWIDEKASAVAGVDFGERENVLE
PFIARSYPLPGGLLKTSSMEGDLLSREVVVVDGIENCMELLDALEMGNVSGRIFEMMACRGGCINGPSMPCDSSLYERRE
RLLSFIENNQQQQGYENIKASAAGIDLRRSFEPKAFKAPRPTESEIREILASIGKTTPEKELNCGACGYPSCRKKAEAVY
HGMAEPDMCIPYMRSRAESLANLIIDHTPNGIILVDSNLNIKEINRAAEKMFGVEKEQVQGKPLFTVIDDRDFAWVLANK
TNLQDKKVNYPRNSLTTLQTMCYIEEEDMVLAIIQDITEQEKQRMELERVREETLEKAQEVINRQMRVAQEIAGLLGETT
AESKMLLLKLIELVKGREEEK

Sequences:

>Translated_581_residues
MGGGLILDVISVSKANCRNCYRCVRHCPVKAIKVVGGQAEVVDSLCIYCGRCVIECPQNAKKVRNDLHAVKEFIYAGEKV
IASIAPSYPAVFDVTSPQEMFSILKALGFYGAEETAVGAEMVSMEYQHLIKKGHKGPIITTACPVVKNLVEKYYPGLIRN
LAPVVSPMVAHARSLKQRYGLDIKVVFIGPCIAKKAEALDRSVLGDVDAVLTFQELADWIDEKASAVAGVDFGERENVLE
PFIARSYPLPGGLLKTSSMEGDLLSREVVVVDGIENCMELLDALEMGNVSGRIFEMMACRGGCINGPSMPCDSSLYERRE
RLLSFIENNQQQQGYENIKASAAGIDLRRSFEPKAFKAPRPTESEIREILASIGKTTPEKELNCGACGYPSCRKKAEAVY
HGMAEPDMCIPYMRSRAESLANLIIDHTPNGIILVDSNLNIKEINRAAEKMFGVEKEQVQGKPLFTVIDDRDFAWVLANK
TNLQDKKVNYPRNSLTTLQTMCYIEEEDMVLAIIQDITEQEKQRMELERVREETLEKAQEVINRQMRVAQEIAGLLGETT
AESKMLLLKLIELVKGREEEK
>Mature_580_residues
GGGLILDVISVSKANCRNCYRCVRHCPVKAIKVVGGQAEVVDSLCIYCGRCVIECPQNAKKVRNDLHAVKEFIYAGEKVI
ASIAPSYPAVFDVTSPQEMFSILKALGFYGAEETAVGAEMVSMEYQHLIKKGHKGPIITTACPVVKNLVEKYYPGLIRNL
APVVSPMVAHARSLKQRYGLDIKVVFIGPCIAKKAEALDRSVLGDVDAVLTFQELADWIDEKASAVAGVDFGERENVLEP
FIARSYPLPGGLLKTSSMEGDLLSREVVVVDGIENCMELLDALEMGNVSGRIFEMMACRGGCINGPSMPCDSSLYERRER
LLSFIENNQQQQGYENIKASAAGIDLRRSFEPKAFKAPRPTESEIREILASIGKTTPEKELNCGACGYPSCRKKAEAVYH
GMAEPDMCIPYMRSRAESLANLIIDHTPNGIILVDSNLNIKEINRAAEKMFGVEKEQVQGKPLFTVIDDRDFAWVLANKT
NLQDKKVNYPRNSLTTLQTMCYIEEEDMVLAIIQDITEQEKQRMELERVREETLEKAQEVINRQMRVAQEIAGLLGETTA
ESKMLLLKLIELVKGREEEK

Specific function: Unknown

COG id: COG4624

COG function: function code R; Iron only hydrogenase large subunit, C-terminal domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012675
- InterPro:   IPR009016
- InterPro:   IPR004108
- InterPro:   IPR003149
- InterPro:   IPR013352
- InterPro:   IPR001041 [H]

Pfam domain/function: PF02906 Fe_hyd_lg_C; PF02256 Fe_hyd_SSU [H]

EC number: =1.12.7.2 [H]

Molecular weight: Translated: 64531; Mature: 64399

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS50112 PAS ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGGLILDVISVSKANCRNCYRCVRHCPVKAIKVVGGQAEVVDSLCIYCGRCVIECPQNA
CCCCEEEHHHHCCCHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHH
KKVRNDLHAVKEFIYAGEKVIASIAPSYPAVFDVTSPQEMFSILKALGFYGAEETAVGAE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCCCCHHHHHHH
MVSMEYQHLIKKGHKGPIITTACPVVKNLVEKYYPGLIRNLAPVVSPMVAHARSLKQRYG
HHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LDIKVVFIGPCIAKKAEALDRSVLGDVDAVLTFQELADWIDEKASAVAGVDFGERENVLE
CCEEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
PFIARSYPLPGGLLKTSSMEGDLLSREVVVVDGIENCMELLDALEMGNVSGRIFEMMACR
HHHHHCCCCCCCCEECCCCCCCHHCCCEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHC
GGCINGPSMPCDSSLYERRERLLSFIENNQQQQGYENIKASAAGIDLRRSFEPKAFKAPR
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCC
PTESEIREILASIGKTTPEKELNCGACGYPSCRKKAEAVYHGMAEPDMCIPYMRSRAESL
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ANLIIDHTPNGIILVDSNLNIKEINRAAEKMFGVEKEQVQGKPLFTVIDDRDFAWVLANK
HHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCHHHHCCCCEEEEECCCCEEEEEECC
TNLQDKKVNYPRNSLTTLQTMCYIEEEDMVLAIIQDITEQEKQRMELERVREETLEKAQE
CCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VINRQMRVAQEIAGLLGETTAESKMLLLKLIELVKGREEEK
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GGGLILDVISVSKANCRNCYRCVRHCPVKAIKVVGGQAEVVDSLCIYCGRCVIECPQNA
CCCEEEHHHHCCCHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHH
KKVRNDLHAVKEFIYAGEKVIASIAPSYPAVFDVTSPQEMFSILKALGFYGAEETAVGAE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCCCCHHHHHHH
MVSMEYQHLIKKGHKGPIITTACPVVKNLVEKYYPGLIRNLAPVVSPMVAHARSLKQRYG
HHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LDIKVVFIGPCIAKKAEALDRSVLGDVDAVLTFQELADWIDEKASAVAGVDFGERENVLE
CCEEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
PFIARSYPLPGGLLKTSSMEGDLLSREVVVVDGIENCMELLDALEMGNVSGRIFEMMACR
HHHHHCCCCCCCCEECCCCCCCHHCCCEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHC
GGCINGPSMPCDSSLYERRERLLSFIENNQQQQGYENIKASAAGIDLRRSFEPKAFKAPR
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCC
PTESEIREILASIGKTTPEKELNCGACGYPSCRKKAEAVYHGMAEPDMCIPYMRSRAESL
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ANLIIDHTPNGIILVDSNLNIKEINRAAEKMFGVEKEQVQGKPLFTVIDDRDFAWVLANK
HHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCHHHHCCCCEEEEECCCCEEEEEECC
TNLQDKKVNYPRNSLTTLQTMCYIEEEDMVLAIIQDITEQEKQRMELERVREETLEKAQE
CCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VINRQMRVAQEIAGLLGETTAESKMLLLKLIELVKGREEEK
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1911757; 9836629; 10529166 [H]