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The map label for this gene is tig

Identifier: 20807118

GI number: 20807118

Start: 629038

End: 630336

Strand: Direct

Name: tig

Synonym: TTE0624

Alternate gene names: 20807118

Gene position: 629038-630336 (Clockwise)

Preceding gene: 20807117

Following gene: 20807119

Centisome position: 23.39

GC content: 36.49

Gene sequence:

>1299_bases
ATGGGTGCGAGTCTTAAGAAAATAGAAAAGAGCGTGGCTACTCTTGAACTTACAATACCAAAAGAAAAATTTGAAGAAGG
CTTAGATTATGCTTTTAAGAAAAATGCTTCAAAATTCAATGTTCCAGGTTTCAGAAAAGGGAAAGCTCCAAGATTTTTAG
TTGAGAGATATTATGGAGAAGGCGTACTTTATGAAGATGCCATAGAGTATGTGTTCCACGAAGCGTACCAGGAAGCTTTA
AAAACGTTTAATTTAGAGCCTGTAGATTATCCAGACATCAACATCTTGCAAATAGGCAAAGGAAAAGATTTGGTTTTAGA
AGCAACTGTTGCAGTTATGCCCGAAGTTGAGCTTGGAGAATACAAGGGCATAGAAATTGAAAAAATAGAATATGATGTGT
ATGATGGAGATGTAGAGTACGAATTAGAGAAGTTAAGGCAGCAAAATGCAAGAATTATCCCAGTTGAGGGAAGACCCGCA
GAGCAGGGGGATATTGCTGTAATTGACTTTGAAGGCTATATAGACGATAAGCCTTTTGAAGGAGGAAAGGGAGAGAATTA
CGAACTTGAATTAGGAAGCAATACTTTTGTGCCTGGCTTTGAAGATCAAATAATAAGCCACAATGTAGGAGAGACTTTCG
ATGTGACTGTTACTTTCCCTGAGGATTACAGAGTGGAAGAATTAAAAGGAAAGACTGCCGTATTCAAAGTTACGCTGAAG
GCATTAAATAAAAAAGAATTGCCAGAACTGGATGATGAGTTTGCGAAAGATGTAAGTGAATTTGAGACTTTGGAAGAGTT
AAAACAAGATATAAGGAAGAAATTAGAAGAGAAAAACAAAAGAGAAGCAGAGAATGAGATGAAAGAAAAAGCTGTCATGA
AAGTAGTAGAAAACGCCAAAGTGGATATTCCTGACGTAATGGTAGAAAGGCAGATAGACCTTTCTTTGAGGGATTTGGAC
TATAATTTGAGATTACAAGGTTTAGATTTAAACACCTATCTATCTATAACCGGCAAGACCATACAGGACTTAAGAAAGGA
AATGTGGGAAGGCGCTTTAAATAGGGTAAAAACCCAGCTTGTGATAGATAAAATAGCGAAAGTTGAAAACATTGAAGCCA
CAGAAGAAGAGCTGGAGAACAAATTAAAAGAGTTGGCAGAGAGTTACAGAGTAAATCTGGAAGAATTCAAGAAAAGTTTA
ACAGAAAGCCAGATAAACGGCATAAAAGAGGATATAGCGTATTATAAGACGATTGACTTTATTTTCAACCAGTGTAAAAT
AGTAAGTAAAGAGGAGTGA

Upstream 100 bases:

>100_bases
TATTTTTTTTGTATTTATTTTGCTTCACAAATTTTTTATGTTATAATATTATAAGTTCAGAAGAATCCACACGAAAGAAA
GGAACAGGAGGATCTACTTA

Downstream 100 bases:

>100_bases
TTTTTATGAGCTTAGTTCCAATTGTAGTGGAGCAGACAAATAGAGGCGAAAGAGCTTATGATATTTTTTCCCGCCTCTTA
AAAGACCGCATAGTGTTTTT

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MGASLKKIEKSVATLELTIPKEKFEEGLDYAFKKNASKFNVPGFRKGKAPRFLVERYYGEGVLYEDAIEYVFHEAYQEAL
KTFNLEPVDYPDINILQIGKGKDLVLEATVAVMPEVELGEYKGIEIEKIEYDVYDGDVEYELEKLRQQNARIIPVEGRPA
EQGDIAVIDFEGYIDDKPFEGGKGENYELELGSNTFVPGFEDQIISHNVGETFDVTVTFPEDYRVEELKGKTAVFKVTLK
ALNKKELPELDDEFAKDVSEFETLEELKQDIRKKLEEKNKREAENEMKEKAVMKVVENAKVDIPDVMVERQIDLSLRDLD
YNLRLQGLDLNTYLSITGKTIQDLRKEMWEGALNRVKTQLVIDKIAKVENIEATEEELENKLKELAESYRVNLEEFKKSL
TESQINGIKEDIAYYKTIDFIFNQCKIVSKEE

Sequences:

>Translated_432_residues
MGASLKKIEKSVATLELTIPKEKFEEGLDYAFKKNASKFNVPGFRKGKAPRFLVERYYGEGVLYEDAIEYVFHEAYQEAL
KTFNLEPVDYPDINILQIGKGKDLVLEATVAVMPEVELGEYKGIEIEKIEYDVYDGDVEYELEKLRQQNARIIPVEGRPA
EQGDIAVIDFEGYIDDKPFEGGKGENYELELGSNTFVPGFEDQIISHNVGETFDVTVTFPEDYRVEELKGKTAVFKVTLK
ALNKKELPELDDEFAKDVSEFETLEELKQDIRKKLEEKNKREAENEMKEKAVMKVVENAKVDIPDVMVERQIDLSLRDLD
YNLRLQGLDLNTYLSITGKTIQDLRKEMWEGALNRVKTQLVIDKIAKVENIEATEEELENKLKELAESYRVNLEEFKKSL
TESQINGIKEDIAYYKTIDFIFNQCKIVSKEE
>Mature_431_residues
GASLKKIEKSVATLELTIPKEKFEEGLDYAFKKNASKFNVPGFRKGKAPRFLVERYYGEGVLYEDAIEYVFHEAYQEALK
TFNLEPVDYPDINILQIGKGKDLVLEATVAVMPEVELGEYKGIEIEKIEYDVYDGDVEYELEKLRQQNARIIPVEGRPAE
QGDIAVIDFEGYIDDKPFEGGKGENYELELGSNTFVPGFEDQIISHNVGETFDVTVTFPEDYRVEELKGKTAVFKVTLKA
LNKKELPELDDEFAKDVSEFETLEELKQDIRKKLEEKNKREAENEMKEKAVMKVVENAKVDIPDVMVERQIDLSLRDLDY
NLRLQGLDLNTYLSITGKTIQDLRKEMWEGALNRVKTQLVIDKIAKVENIEATEEELENKLKELAESYRVNLEEFKKSLT
ESQINGIKEDIAYYKTIDFIFNQCKIVSKEE

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=375, Percent_Identity=32.2666666666667, Blast_Score=176, Evalue=4e-45,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_THETN (Q8RC26)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_622289.1
- ProteinModelPortal:   Q8RC26
- GeneID:   996554
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE0624
- NMPDR:   fig|273068.3.peg.207
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- ProtClustDB:   PRK01490
- BioCyc:   TTEN273068:TTE0624-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 49763; Mature: 49632

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGASLKKIEKSVATLELTIPKEKFEEGLDYAFKKNASKFNVPGFRKGKAPRFLVERYYGE
CCCHHHHHHHHHHEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCC
GVLYEDAIEYVFHEAYQEALKTFNLEPVDYPDINILQIGKGKDLVLEATVAVMPEVELGE
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCEEEEEHHHHCCCCCCCC
YKGIEIEKIEYDVYDGDVEYELEKLRQQNARIIPVEGRPAEQGDIAVIDFEGYIDDKPFE
CCCCEEEEEEEEEECCCHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCC
GGKGENYELELGSNTFVPGFEDQIISHNVGETFDVTVTFPEDYRVEELKGKTAVFKVTLK
CCCCCCEEEEECCCCCCCCCHHHHHCCCCCCEEEEEEECCCCCCHHHHCCCHHHEEHHHH
ALNKKELPELDDEFAKDVSEFETLEELKQDIRKKLEEKNKREAENEMKEKAVMKVVENAK
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VDIPDVMVERQIDLSLRDLDYNLRLQGLDLNTYLSITGKTIQDLRKEMWEGALNRVKTQL
CCCCHHHHHHHCCCEEEECCCEEEEEEECEEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
VIDKIAKVENIEATEEELENKLKELAESYRVNLEEFKKSLTESQINGIKEDIAYYKTIDF
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFNQCKIVSKEE
HHHHCEECCCCC
>Mature Secondary Structure 
GASLKKIEKSVATLELTIPKEKFEEGLDYAFKKNASKFNVPGFRKGKAPRFLVERYYGE
CCHHHHHHHHHHEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCC
GVLYEDAIEYVFHEAYQEALKTFNLEPVDYPDINILQIGKGKDLVLEATVAVMPEVELGE
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCEEEEEHHHHCCCCCCCC
YKGIEIEKIEYDVYDGDVEYELEKLRQQNARIIPVEGRPAEQGDIAVIDFEGYIDDKPFE
CCCCEEEEEEEEEECCCHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCC
GGKGENYELELGSNTFVPGFEDQIISHNVGETFDVTVTFPEDYRVEELKGKTAVFKVTLK
CCCCCCEEEEECCCCCCCCCHHHHHCCCCCCEEEEEEECCCCCCHHHHCCCHHHEEHHHH
ALNKKELPELDDEFAKDVSEFETLEELKQDIRKKLEEKNKREAENEMKEKAVMKVVENAK
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VDIPDVMVERQIDLSLRDLDYNLRLQGLDLNTYLSITGKTIQDLRKEMWEGALNRVKTQL
CCCCHHHHHHHCCCEEEECCCEEEEEEECEEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
VIDKIAKVENIEATEEELENKLKELAESYRVNLEEFKKSLTESQINGIKEDIAYYKTIDF
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFNQCKIVSKEE
HHHHCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336