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The map label for this gene is rph

Identifier: 20807113

GI number: 20807113

Start: 624593

End: 625378

Strand: Direct

Name: rph

Synonym: TTE0618

Alternate gene names: 20807113

Gene position: 624593-625378 (Clockwise)

Preceding gene: 20807111

Following gene: 20807114

Centisome position: 23.22

GC content: 41.22

Gene sequence:

>786_bases
ATGAACAGAATAGATGGAAGGGAATTTAATGAATTAAGACCAATAAAGATTACCAGAAATTTTAATAAATTTGCTGAAGG
GTCTGTATTAATTGAAATGGGTGAGACAAAAGTAATATGCACTGCTTCTATTGAGGACAAAGTTCCGCCTTTCCAAAAAG
GTACTGGAAAGGGGTGGATTACAAGCGAATACGGCATGTTGCCTAGGGCAACAGAAGTGAGAAATCCCAGAGAGGTTACA
AAAGGAAGGCCTTCTGGAAGGACAATGGAGATACAGCGCCTAATTGGGCGATCCCTCCGAGCTGTTGTAGATTTAGAAGC
TTTGGGGGAGAGGACAATTTGGATTGACTGCGATGTAATACAAGCAGACGGAGGAACCCGAACAGCTTCTATAACTGGGT
CTTTTATAGCTTTAGCTGATGCTCTCAATAAATTGGTAGAAAAAGGGGAATTACAGAAGATACCTTTGAAAAGTTTTGTT
GCAGCAGTAAGCGTGGGCATTGTAGAAGGAAATAAACTTTTAGACCTTTCTTTCCAGGAAGATGCCAATGCTCTTGTGGA
CATGAATGTGGTTATGACAGACAAAGGAGAGATTGTAGAGATACAGGGCACAGGAGAAGGAGGACCTTTTTCAAGGCAAA
ATTTTGAGGAGCTTTTGGATTTAGCTGCTCATGGCATTGAACAGATTATCAAGATACAGAAAGAAGTGCTCTCAGACATT
GCTGACAAAATAGGGGTGGAGAATGTTGAGGTTGATAGTGGCAACTCACAATCCCAACAAGGCTAA

Upstream 100 bases:

>100_bases
ATTTTTTGCAGAGCATAAATAAAATAATCTGTTCTTGTAAAATAGTAATAAAGGTTTTAAAATATAAATGTAGATAAATA
GAAGGGAAAGGGTTGAAGAA

Downstream 100 bases:

>100_bases
AGAGATAAAAGACTTTTTTAAAGGTTACCCTGTGGAAGTAGTCTCAATGAAAGAATTGGGAATAGAAGAAGATATTGAAG
AATATGGCAATACAATCGAA

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MNRIDGREFNELRPIKITRNFNKFAEGSVLIEMGETKVICTASIEDKVPPFQKGTGKGWITSEYGMLPRATEVRNPREVT
KGRPSGRTMEIQRLIGRSLRAVVDLEALGERTIWIDCDVIQADGGTRTASITGSFIALADALNKLVEKGELQKIPLKSFV
AAVSVGIVEGNKLLDLSFQEDANALVDMNVVMTDKGEIVEIQGTGEGGPFSRQNFEELLDLAAHGIEQIIKIQKEVLSDI
ADKIGVENVEVDSGNSQSQQG

Sequences:

>Translated_261_residues
MNRIDGREFNELRPIKITRNFNKFAEGSVLIEMGETKVICTASIEDKVPPFQKGTGKGWITSEYGMLPRATEVRNPREVT
KGRPSGRTMEIQRLIGRSLRAVVDLEALGERTIWIDCDVIQADGGTRTASITGSFIALADALNKLVEKGELQKIPLKSFV
AAVSVGIVEGNKLLDLSFQEDANALVDMNVVMTDKGEIVEIQGTGEGGPFSRQNFEELLDLAAHGIEQIIKIQKEVLSDI
ADKIGVENVEVDSGNSQSQQG
>Mature_261_residues
MNRIDGREFNELRPIKITRNFNKFAEGSVLIEMGETKVICTASIEDKVPPFQKGTGKGWITSEYGMLPRATEVRNPREVT
KGRPSGRTMEIQRLIGRSLRAVVDLEALGERTIWIDCDVIQADGGTRTASITGSFIALADALNKLVEKGELQKIPLKSFV
AAVSVGIVEGNKLLDLSFQEDANALVDMNVVMTDKGEIVEIQGTGEGGPFSRQNFEELLDLAAHGIEQIIKIQKEVLSDI
ADKIGVENVEVDSGNSQSQQG

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=210, Percent_Identity=64.7619047619048, Blast_Score=285, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI71981632, Length=183, Percent_Identity=30.0546448087432, Blast_Score=84, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_THETN (Q8RC30)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_622284.1
- ProteinModelPortal:   Q8RC30
- SMR:   Q8RC30
- GeneID:   996540
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE0618
- NMPDR:   fig|273068.3.peg.202
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- ProtClustDB:   PRK00173
- BioCyc:   TTEN273068:TTE0618-MONOMER
- BRENDA:   2.7.7.56
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 28515; Mature: 28515

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIDGREFNELRPIKITRNFNKFAEGSVLIEMGETKVICTASIEDKVPPFQKGTGKGWI
CCCCCCCCCCCCCCEEEECCCCHHCCCEEEEEECCEEEEEEECCCCCCCCCCCCCCCCEE
TSEYGMLPRATEVRNPREVTKGRPSGRTMEIQRLIGRSLRAVVDLEALGERTIWIDCDVI
ECCCCCCCCHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEE
QADGGTRTASITGSFIALADALNKLVEKGELQKIPLKSFVAAVSVGIVEGNKLLDLSFQE
ECCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHEEECCCEEEEEECCC
DANALVDMNVVMTDKGEIVEIQGTGEGGPFSRQNFEELLDLAAHGIEQIIKIQKEVLSDI
CCCEEEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADKIGVENVEVDSGNSQSQQG
HHHHCCCEEEECCCCCCCCCC
>Mature Secondary Structure
MNRIDGREFNELRPIKITRNFNKFAEGSVLIEMGETKVICTASIEDKVPPFQKGTGKGWI
CCCCCCCCCCCCCCEEEECCCCHHCCCEEEEEECCEEEEEEECCCCCCCCCCCCCCCCEE
TSEYGMLPRATEVRNPREVTKGRPSGRTMEIQRLIGRSLRAVVDLEALGERTIWIDCDVI
ECCCCCCCCHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEE
QADGGTRTASITGSFIALADALNKLVEKGELQKIPLKSFVAAVSVGIVEGNKLLDLSFQE
ECCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHEEECCCEEEEEECCC
DANALVDMNVVMTDKGEIVEIQGTGEGGPFSRQNFEELLDLAAHGIEQIIKIQKEVLSDI
CCCEEEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADKIGVENVEVDSGNSQSQQG
HHHHCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336