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The map label for this gene is PurD

Identifier: 20807088

GI number: 20807088

Start: 594679

End: 595932

Strand: Direct

Name: PurD

Synonym: TTE0593

Alternate gene names: 20807088

Gene position: 594679-595932 (Clockwise)

Preceding gene: 20807087

Following gene: 20807089

Centisome position: 22.11

GC content: 38.6

Gene sequence:

>1254_bases
TTGAAAGTATTAGTAGTTGGGGGAGGAGGAAGAGAACACGCAATTGTGCACAAGCTTTCCCAAAGCGATCGGGTAGAAAA
AATTTACTGTGCACCTGGAAATGCAGGGATTGGGCAGCTAGCAGAGTGCGTAAATATAAGTGTAGAAGAAATAGAGAAGC
TAAAAGAGTTTGCTTTACAAAATAAAATAGATATAACAATAGTGGGACCGGAGCTTCCATTAGTAGAGGGAATTGTGGAT
GAGTTTGAAAGAACTGGACTTAAGATTTTTGGTCCGAGCAAAAAAGCAGCCATGATAGAAGGGAGCAAGTACTTTGCAAA
ACAGATCATGGCAAAGTATGAAATACCTACAGGAAGATTTAAAGCTTTTGACAGGTATCAAGAAGCCCTGAAATTCTTAA
AGGAAACATGGTATCCTGTTGTGATAAAAGCTGATGGATTAGCGCAAGGAAAAGGGGTTTTCATAGTAAGAGACTTTATT
GAGGCGAAAGAGGTTCTTGACCTCATGATGAAGAAAAGAGTTTTCGGGCCATCAGGGGATATAGTAATTGTGGAGGAGAT
GCTTTACGGCAAAGAAGCTTCTATATTTGCCTTTGTGGATGGGGAAAATGTTTTGACCATGATGACTGCTATGGATTATA
AAAAAGTTTATGAAAAAGATGAGGGACCAAATACGGGAGGTATGGGAAGCATTGCCCCAAATCCTCACATAGATAAAAAG
ACTTTAAATGAAATTGAAGAGAAAATTTTAAAACCTGTTGTGTATGCGCTTAAGAAAGAGGGAATTGTGTATAAAGGCGT
TTTGTATGCAGGATTAATGCTGACAAAAGAAGGACCCAAAGTTCTAGAATTTAATGCGAGATTCGGGGACCCTGAGACAC
AAGCAATACTTCCACTTCTTAAAACAGATTTTTTGGAAATAATAGAAGCTACGCTGGAAGGAAAGCTTAAAAATTTGAAG
CTGGAGTGGGAAGATAAAAAGGCTGTATGTGTTATTGCAGCTTCAAAAGGATATCCTGGAGAATATAAAAAGGGCTTTGA
AATACGGGGATTGGAAGAAGTGAAGGAAGCCTTTGTCTACCACGCAGGTACTTCTTTTAAAGATGGAAAGATTGTCACTT
CAGGAGGAAGAGTCTTTGGAATTGTGGCTTTAGGGGATTCCTATAAAGAGGCAAGGGAAATAGCATATAGAGAAATTGAA
AAAATATCTTTTGAAGGAATTTATTACAGAAAGGATATCGCGGCCGGGTACTAA

Upstream 100 bases:

>100_bases
TCCCAGAATGACCAGCTTTCAATTGAGGCAGCTGACAGAGCAGGTATTGCGATGATTTTTACTGGAATAAGGCATTTTAA
GCATTGAGGAGGGGTTGACT

Downstream 100 bases:

>100_bases
CCCGGCAATTTTTATGGTATAATACAAATAAAATGGGTTTCAAAAGGCGGTGGACATAATGCCTAAAGACCATGTCAAAC
TCTTTGATACCATCTCTCCC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MKVLVVGGGGREHAIVHKLSQSDRVEKIYCAPGNAGIGQLAECVNISVEEIEKLKEFALQNKIDITIVGPELPLVEGIVD
EFERTGLKIFGPSKKAAMIEGSKYFAKQIMAKYEIPTGRFKAFDRYQEALKFLKETWYPVVIKADGLAQGKGVFIVRDFI
EAKEVLDLMMKKRVFGPSGDIVIVEEMLYGKEASIFAFVDGENVLTMMTAMDYKKVYEKDEGPNTGGMGSIAPNPHIDKK
TLNEIEEKILKPVVYALKKEGIVYKGVLYAGLMLTKEGPKVLEFNARFGDPETQAILPLLKTDFLEIIEATLEGKLKNLK
LEWEDKKAVCVIAASKGYPGEYKKGFEIRGLEEVKEAFVYHAGTSFKDGKIVTSGGRVFGIVALGDSYKEAREIAYREIE
KISFEGIYYRKDIAAGY

Sequences:

>Translated_417_residues
MKVLVVGGGGREHAIVHKLSQSDRVEKIYCAPGNAGIGQLAECVNISVEEIEKLKEFALQNKIDITIVGPELPLVEGIVD
EFERTGLKIFGPSKKAAMIEGSKYFAKQIMAKYEIPTGRFKAFDRYQEALKFLKETWYPVVIKADGLAQGKGVFIVRDFI
EAKEVLDLMMKKRVFGPSGDIVIVEEMLYGKEASIFAFVDGENVLTMMTAMDYKKVYEKDEGPNTGGMGSIAPNPHIDKK
TLNEIEEKILKPVVYALKKEGIVYKGVLYAGLMLTKEGPKVLEFNARFGDPETQAILPLLKTDFLEIIEATLEGKLKNLK
LEWEDKKAVCVIAASKGYPGEYKKGFEIRGLEEVKEAFVYHAGTSFKDGKIVTSGGRVFGIVALGDSYKEAREIAYREIE
KISFEGIYYRKDIAAGY
>Mature_417_residues
MKVLVVGGGGREHAIVHKLSQSDRVEKIYCAPGNAGIGQLAECVNISVEEIEKLKEFALQNKIDITIVGPELPLVEGIVD
EFERTGLKIFGPSKKAAMIEGSKYFAKQIMAKYEIPTGRFKAFDRYQEALKFLKETWYPVVIKADGLAQGKGVFIVRDFI
EAKEVLDLMMKKRVFGPSGDIVIVEEMLYGKEASIFAFVDGENVLTMMTAMDYKKVYEKDEGPNTGGMGSIAPNPHIDKK
TLNEIEEKILKPVVYALKKEGIVYKGVLYAGLMLTKEGPKVLEFNARFGDPETQAILPLLKTDFLEIIEATLEGKLKNLK
LEWEDKKAVCVIAASKGYPGEYKKGFEIRGLEEVKEAFVYHAGTSFKDGKIVTSGGRVFGIVALGDSYKEAREIAYREIE
KISFEGIYYRKDIAAGY

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI28416899, Length=418, Percent_Identity=44.9760765550239, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI4503915, Length=418, Percent_Identity=44.9760765550239, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI209869995, Length=418, Percent_Identity=44.9760765550239, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI209869993, Length=418, Percent_Identity=44.9760765550239, Blast_Score=377, Evalue=1e-104,
Organism=Escherichia coli, GI1790438, Length=419, Percent_Identity=47.0167064439141, Blast_Score=379, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17567511, Length=426, Percent_Identity=39.906103286385, Blast_Score=286, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6321203, Length=436, Percent_Identity=38.0733944954128, Blast_Score=305, Evalue=8e-84,
Organism=Drosophila melanogaster, GI62471619, Length=425, Percent_Identity=43.5294117647059, Blast_Score=327, Evalue=9e-90,
Organism=Drosophila melanogaster, GI24582400, Length=425, Percent_Identity=43.5294117647059, Blast_Score=320, Evalue=2e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR2_THETN (Q8RC54)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_622259.1
- ProteinModelPortal:   Q8RC54
- SMR:   Q8RC54
- GeneID:   996383
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE0593
- NMPDR:   fig|273068.3.peg.70
- HOGENOM:   HBG404060
- OMA:   GVPIVIK
- ProtClustDB:   PRK00885
- BioCyc:   TTEN273068:TTE0593-MONOMER
- BRENDA:   6.3.4.13
- HAMAP:   MF_00138
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.90.600.10
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR00877

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.13

Molecular weight: Translated: 46437; Mature: 46437

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS50975 ATP_GRASP; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVVGGGGREHAIVHKLSQSDRVEKIYCAPGNAGIGQLAECVNISVEEIEKLKEFALQ
CEEEEECCCCCCHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHC
NKIDITIVGPELPLVEGIVDEFERTGLKIFGPSKKAAMIEGSKYFAKQIMAKYEIPTGRF
CCCEEEEECCCCHHHHHHHHHHHHCCEEEECCCCCCEEECCHHHHHHHHHHHCCCCCCHH
KAFDRYQEALKFLKETWYPVVIKADGLAQGKGVFIVRDFIEAKEVLDLMMKKRVFGPSGD
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCC
IVIVEEMLYGKEASIFAFVDGENVLTMMTAMDYKKVYEKDEGPNTGGMGSIAPNPHIDKK
EEEEEHHHCCCCCCEEEEECCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
TLNEIEEKILKPVVYALKKEGIVYKGVLYAGLMLTKEGPKVLEFNARFGDPETQAILPLL
HHHHHHHHHHHHHHHHHHHCCEEEEHHHHHCEEEECCCCCEEEEECCCCCCHHHHHHHHH
KTDFLEIIEATLEGKLKNLKLEWEDKKAVCVIAASKGYPGEYKKGFEIRGLEEVKEAFVY
HHHHHHHHHHHHCCHHHCCEEEECCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHE
HAGTSFKDGKIVTSGGRVFGIVALGDSYKEAREIAYREIEKISFEGIYYRKDIAAGY
ECCCCCCCCEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCC
>Mature Secondary Structure
MKVLVVGGGGREHAIVHKLSQSDRVEKIYCAPGNAGIGQLAECVNISVEEIEKLKEFALQ
CEEEEECCCCCCHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHC
NKIDITIVGPELPLVEGIVDEFERTGLKIFGPSKKAAMIEGSKYFAKQIMAKYEIPTGRF
CCCEEEEECCCCHHHHHHHHHHHHCCEEEECCCCCCEEECCHHHHHHHHHHHCCCCCCHH
KAFDRYQEALKFLKETWYPVVIKADGLAQGKGVFIVRDFIEAKEVLDLMMKKRVFGPSGD
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCC
IVIVEEMLYGKEASIFAFVDGENVLTMMTAMDYKKVYEKDEGPNTGGMGSIAPNPHIDKK
EEEEEHHHCCCCCCEEEEECCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
TLNEIEEKILKPVVYALKKEGIVYKGVLYAGLMLTKEGPKVLEFNARFGDPETQAILPLL
HHHHHHHHHHHHHHHHHHHCCEEEEHHHHHCEEEECCCCCEEEEECCCCCCHHHHHHHHH
KTDFLEIIEATLEGKLKNLKLEWEDKKAVCVIAASKGYPGEYKKGFEIRGLEEVKEAFVY
HHHHHHHHHHHHCCHHHCCEEEECCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHE
HAGTSFKDGKIVTSGGRVFGIVALGDSYKEAREIAYREIEKISFEGIYYRKDIAAGY
ECCCCCCCCEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336