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The map label for this gene is AprE [H]

Identifier: 20807072

GI number: 20807072

Start: 576766

End: 578004

Strand: Reverse

Name: AprE [H]

Synonym: TTE0576

Alternate gene names: 20807072

Gene position: 578004-576766 (Counterclockwise)

Preceding gene: 20807077

Following gene: 20807041

Centisome position: 21.49

GC content: 39.39

Gene sequence:

>1239_bases
GTGAATATAATCCCTTTATTTGCCTTCTCAAAAATCGCAAAGGAAATCTATACTTTGCAAAAAATTGATAAAAGGCTTTT
AAGCAAAGCTTCTTACTTGAGGAGTGAATGTGTACCTGCAATTTTATATTCAAATCTTCCTTATGAAACCTTAAAGTCTA
AAATAGAAAAATTTGGCGGGAGCATTAAGTTTGAGCTTCCCATAATAAAAGGATGGTCAGTAAATTTGCCCTGCGATAAG
CTAAAGCATTTTGCTTCCATTAAAGGAATCCACTTTATAGCAGAAGACTCTCTCGTAAAACTACAGCTTCACATAGCTAC
TCAGGAAATCGCATCCAGAAAAGCAAATGACTTAGGATATACCGGAAAGGGAATAACTATAGCTTTTTTAGACACAGGGA
TATATCCACACCCTGATTTTACAAAGCCTAAAAACCGCATAATCGCTTTTTATGACGTAGTAAATGGGAAAAAGCAGCCC
TATGACGACAACGGTCACGGAACTCACGTGGCAGGAGATGCAGCAGGAAACGGATATGCTTCTAATGGAAAATACAAAGG
AGTTGCCCCTGAAGCAAATATAGTGGCAGTAAAAGTGCTGGACTCCTACGGAAGAAGCAGTTCCTCAGACATTTTGGCAG
GAATGCAATGGGTTTTGGACAACAAGGAGAAATACAACATACGAATCGTCTCCCTTTCAATAGGAGAAACTCCTGCTCTT
CCAACTTTCTTGGACCCTCTGGTGAGAGGGGTAGATACTTTGTGGAAAAATGGAATAGTTGTAGTAGTTGCCGCAGGAAA
TTCAGGTCCTAATTACAATTCCATAACTTCTCCGGGAACAAGTAGAAATGCTATAACGGTAGGAGCAGTGGACGATAAAA
GGACTCCAGATATAGAAGACGACGAAGTAGCCAAATTCTCAGGAAGAGGAGGACCTTATCTTTACAAACCAGATGTAGTA
GCCCCAGGAGTGAAAATAGTCTCTACAGCTTCTGGAAATGTGCCTTTTGGAGCAGATGAGATAATGATAAACAAACCTTA
CAGGAGCGCCACAGGTACTTCAATGGCAACTCCAATGGTAGCAGGAGCAGTAGCATTGCTTTTAGAGAAAAATCCACGTC
TGACAAATGTAGAAATAAAAAATATATTAAAGAACACAGCCACAAAAATTAACGAAGCAGGTCTTTGGACACAAGGAAGT
GGAATGATAAATATAGAAGAGGCTTTAAAAAAAGTTTAA

Upstream 100 bases:

>100_bases
AATATGAAGCAACTTTTTAAGGTGACACTTTTTAAAAAATTCCACTAGCAAATTTTTGTGTCTCTCATATTGTGGTAGTA
GCGAGAAAGGAGGTATAGAT

Downstream 100 bases:

>100_bases
GGAGGGTGTCCCCCTCCTTATCTTATGAGATTGAGTATTAGCTGATAAAAAATGGGTATAAAAATGGAAGGCAGTAAATT
TCCAACTTTTATACTTTTAA

Product: subtilisin-like serine protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MNIIPLFAFSKIAKEIYTLQKIDKRLLSKASYLRSECVPAILYSNLPYETLKSKIEKFGGSIKFELPIIKGWSVNLPCDK
LKHFASIKGIHFIAEDSLVKLQLHIATQEIASRKANDLGYTGKGITIAFLDTGIYPHPDFTKPKNRIIAFYDVVNGKKQP
YDDNGHGTHVAGDAAGNGYASNGKYKGVAPEANIVAVKVLDSYGRSSSSDILAGMQWVLDNKEKYNIRIVSLSIGETPAL
PTFLDPLVRGVDTLWKNGIVVVVAAGNSGPNYNSITSPGTSRNAITVGAVDDKRTPDIEDDEVAKFSGRGGPYLYKPDVV
APGVKIVSTASGNVPFGADEIMINKPYRSATGTSMATPMVAGAVALLLEKNPRLTNVEIKNILKNTATKINEAGLWTQGS
GMINIEEALKKV

Sequences:

>Translated_412_residues
MNIIPLFAFSKIAKEIYTLQKIDKRLLSKASYLRSECVPAILYSNLPYETLKSKIEKFGGSIKFELPIIKGWSVNLPCDK
LKHFASIKGIHFIAEDSLVKLQLHIATQEIASRKANDLGYTGKGITIAFLDTGIYPHPDFTKPKNRIIAFYDVVNGKKQP
YDDNGHGTHVAGDAAGNGYASNGKYKGVAPEANIVAVKVLDSYGRSSSSDILAGMQWVLDNKEKYNIRIVSLSIGETPAL
PTFLDPLVRGVDTLWKNGIVVVVAAGNSGPNYNSITSPGTSRNAITVGAVDDKRTPDIEDDEVAKFSGRGGPYLYKPDVV
APGVKIVSTASGNVPFGADEIMINKPYRSATGTSMATPMVAGAVALLLEKNPRLTNVEIKNILKNTATKINEAGLWTQGS
GMINIEEALKKV
>Mature_412_residues
MNIIPLFAFSKIAKEIYTLQKIDKRLLSKASYLRSECVPAILYSNLPYETLKSKIEKFGGSIKFELPIIKGWSVNLPCDK
LKHFASIKGIHFIAEDSLVKLQLHIATQEIASRKANDLGYTGKGITIAFLDTGIYPHPDFTKPKNRIIAFYDVVNGKKQP
YDDNGHGTHVAGDAAGNGYASNGKYKGVAPEANIVAVKVLDSYGRSSSSDILAGMQWVLDNKEKYNIRIVSLSIGETPAL
PTFLDPLVRGVDTLWKNGIVVVVAAGNSGPNYNSITSPGTSRNAITVGAVDDKRTPDIEDDEVAKFSGRGGPYLYKPDVV
APGVKIVSTASGNVPFGADEIMINKPYRSATGTSMATPMVAGAVALLLEKNPRLTNVEIKNILKNTATKINEAGLWTQGS
GMINIEEALKKV

Specific function: Displays serine protease activity. Seems to have a broad substrate specificity [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Cytoplasm (Probable). Note=Is leaked to the culture medium during the late stationary phase owing to cell lysis [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Homo sapiens, GI4506775, Length=337, Percent_Identity=29.3768545994065, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI186972143, Length=254, Percent_Identity=29.9212598425197, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17533391, Length=260, Percent_Identity=28.0769230769231, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71983555, Length=302, Percent_Identity=29.4701986754967, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25141268, Length=306, Percent_Identity=28.1045751633987, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319893, Length=270, Percent_Identity=33.7037037037037, Blast_Score=106, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6320775, Length=267, Percent_Identity=34.0823970037453, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324576, Length=308, Percent_Identity=30.1948051948052, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45550681, Length=300, Percent_Identity=32.3333333333333, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24653270, Length=264, Percent_Identity=29.1666666666667, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17137360, Length=264, Percent_Identity=29.1666666666667, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500 [H]

Pfam domain/function: PF00082 Peptidase_S8 [H]

EC number: NA

Molecular weight: Translated: 44530; Mature: 44530

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIIPLFAFSKIAKEIYTLQKIDKRLLSKASYLRSECVPAILYSNLPYETLKSKIEKFGG
CCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHCC
SIKFELPIIKGWSVNLPCDKLKHFASIKGIHFIAEDSLVKLQLHIATQEIASRKANDLGY
CEEEEEEEEECCCCCCCHHHHHHHHHHCCEEEEECCCEEEEEEEEHHHHHHHCCCCCCCC
TGKGITIAFLDTGIYPHPDFTKPKNRIIAFYDVVNGKKQPYDDNGHGTHVAGDAAGNGYA
CCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCC
SNGKYKGVAPEANIVAVKVLDSYGRSSSSDILAGMQWVLDNKEKYNIRIVSLSIGETPAL
CCCEECCCCCCCCEEEEEEEHHCCCCCCCHHHHHHHHEECCCCEEEEEEEEEECCCCCCC
PTFLDPLVRGVDTLWKNGIVVVVAAGNSGPNYNSITSPGTSRNAITVGAVDDKRTPDIED
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCC
DEVAKFSGRGGPYLYKPDVVAPGVKIVSTASGNVPFGADEIMINKPYRSATGTSMATPMV
CCHHEECCCCCCEEECCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCCCCHHHHHH
AGAVALLLEKNPRLTNVEIKNILKNTATKINEAGLWTQGSGMINIEEALKKV
HHHHHHEECCCCCEECHHHHHHHHHHHHHHHCCCCEECCCCCEEHHHHHHCC
>Mature Secondary Structure
MNIIPLFAFSKIAKEIYTLQKIDKRLLSKASYLRSECVPAILYSNLPYETLKSKIEKFGG
CCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHCC
SIKFELPIIKGWSVNLPCDKLKHFASIKGIHFIAEDSLVKLQLHIATQEIASRKANDLGY
CEEEEEEEEECCCCCCCHHHHHHHHHHCCEEEEECCCEEEEEEEEHHHHHHHCCCCCCCC
TGKGITIAFLDTGIYPHPDFTKPKNRIIAFYDVVNGKKQPYDDNGHGTHVAGDAAGNGYA
CCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCC
SNGKYKGVAPEANIVAVKVLDSYGRSSSSDILAGMQWVLDNKEKYNIRIVSLSIGETPAL
CCCEECCCCCCCCEEEEEEEHHCCCCCCCHHHHHHHHEECCCCEEEEEEEEEECCCCCCC
PTFLDPLVRGVDTLWKNGIVVVVAAGNSGPNYNSITSPGTSRNAITVGAVDDKRTPDIED
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCC
DEVAKFSGRGGPYLYKPDVVAPGVKIVSTASGNVPFGADEIMINKPYRSATGTSMATPMV
CCHHEECCCCCCEEECCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCCCCHHHHHH
AGAVALLLEKNPRLTNVEIKNILKNTATKINEAGLWTQGSGMINIEEALKKV
HHHHHHEECCCCCEECHHHHHHHHHHHHHHHCCCCEECCCCCEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]