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The map label for this gene is CspR [C]

Identifier: 20806774

GI number: 20806774

Start: 257043

End: 257513

Strand: Direct

Name: CspR [C]

Synonym: TTE0253

Alternate gene names: 20806774

Gene position: 257043-257513 (Clockwise)

Preceding gene: 20806773

Following gene: 20806775

Centisome position: 9.56

GC content: 39.28

Gene sequence:

>471_bases
ATGCCACTTAATGTAGTACTTGTTGAACCTGAGATACCCCAGAATACGGGTAATATTGCAAGAACCTGTGTACTTACAGG
AAGCAGACTTCACCTTGTAAAACCTTTGGGTTTTAGTATAGATGAGAAAGATGTAAGAAGGGCAGGGCTTGACTATTGGC
CCTTATTGGACCTCACGGTATATGAAAACCTTCAAGAGTTTCTGGAAAAGCATAAAGGGAAGAAGTTTTACCTTGCTACT
ACAAGAGCAAACAAATACTACCATGAAGTCAAATATGAAGACGAAGCTTTTTTGATTTTTGGAAAAGAAACGACAGGATT
GCCTCAGTGGCTCATAGACGAATATTACGAGGACTGTATACGCATACCGATGCACGAGAAAATCCTCTGGAGATCTCTTA
ATTTGTCTAATTCCGTTGCCATAATAGTATATGAAGCCTTAAAGCAGTTGGGTTTTCCCAATATGAGATGA

Upstream 100 bases:

>100_bases
AAATATTTGACAACTAGGGAAATAGTGAATTTTTTAATTATAAAGTTTGTTGTGATATAATAGATTTATAAAGTTAGTTG
CACATACAGGGGGTTCAAAA

Downstream 100 bases:

>100_bases
AATGAGGGAAATTTATGAAAAGAAAGATTATCCATGTTGATATGGACGCTTTCTTTGCGTCCATTGAACAACAGGATAAT
CCTGAATACAGAGGGAAGCC

Product: rRNA methylase (SpoU class)

Products: NA

Alternate protein names: ORF3 [H]

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MPLNVVLVEPEIPQNTGNIARTCVLTGSRLHLVKPLGFSIDEKDVRRAGLDYWPLLDLTVYENLQEFLEKHKGKKFYLAT
TRANKYYHEVKYEDEAFLIFGKETTGLPQWLIDEYYEDCIRIPMHEKILWRSLNLSNSVAIIVYEALKQLGFPNMR

Sequences:

>Translated_156_residues
MPLNVVLVEPEIPQNTGNIARTCVLTGSRLHLVKPLGFSIDEKDVRRAGLDYWPLLDLTVYENLQEFLEKHKGKKFYLAT
TRANKYYHEVKYEDEAFLIFGKETTGLPQWLIDEYYEDCIRIPMHEKILWRSLNLSNSVAIIVYEALKQLGFPNMR
>Mature_155_residues
PLNVVLVEPEIPQNTGNIARTCVLTGSRLHLVKPLGFSIDEKDVRRAGLDYWPLLDLTVYENLQEFLEKHKGKKFYLATT
RANKYYHEVKYEDEAFLIFGKETTGLPQWLIDEYYEDCIRIPMHEKILWRSLNLSNSVAIIVYEALKQLGFPNMR

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=153, Percent_Identity=47.0588235294118, Blast_Score=145, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 18249; Mature: 18118

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLNVVLVEPEIPQNTGNIARTCVLTGSRLHLVKPLGFSIDEKDVRRAGLDYWPLLDLTV
CCCEEEEECCCCCCCCCCEEEEEEEECCEEEEEECCCCCCCHHHHHHCCCCCCCHHHHHH
YENLQEFLEKHKGKKFYLATTRANKYYHEVKYEDEAFLIFGKETTGLPQWLIDEYYEDCI
HHHHHHHHHHHCCCEEEEEEECCCCEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHH
RIPMHEKILWRSLNLSNSVAIIVYEALKQLGFPNMR
CCCCCHHHHHHHCCCCCCEEEHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PLNVVLVEPEIPQNTGNIARTCVLTGSRLHLVKPLGFSIDEKDVRRAGLDYWPLLDLTV
CCEEEEECCCCCCCCCCEEEEEEEECCEEEEEECCCCCCCHHHHHHCCCCCCCHHHHHH
YENLQEFLEKHKGKKFYLATTRANKYYHEVKYEDEAFLIFGKETTGLPQWLIDEYYEDCI
HHHHHHHHHHHCCCEEEEEEECCCCEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHH
RIPMHEKILWRSLNLSNSVAIIVYEALKQLGFPNMR
CCCCCHHHHHHHCCCCCCEEEHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1339360 [H]